(Linear) (Six-base) MAPSORT of: KIEE6723.seq Check: 5772 from: 1 to: 2097 KIEE6723.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. Acc65I G'GTAC_C Cuts at: 0 949 2097 Size: 949 1148 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 119 539 564 1129 1453 2097 Size: 119 420 25 565 324 644 Fragments arranged by size: 644 565 420 324 119 25 AflIII A'CryG_T Cuts at: 0 1133 1529 1619 2097 Size: 1133 396 90 478 Fragments arranged by size: 1133 478 396 90 AhdI GACnn_n'nnGTC Cuts at: 0 1635 2097 Size: 1635 462 AleI CACnn'nnGTG Cuts at: 0 1900 2097 Size: 1900 197 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1111 2097 Size: 1111 986 AlwNI CAG_nnn'CTG Cuts at: 0 97 1300 2097 Size: 97 1203 797 Fragments arranged by size: 1203 797 97 ApaI G_GGCC'C Cuts at: 0 129 2097 Size: 129 1968 BanI G'GyrC_C Cuts at: 0 949 1770 2097 Size: 949 821 327 Fragments arranged by size: 949 821 327 BanII G_rGCy'C Cuts at: 0 129 449 2097 Size: 129 320 1648 Fragments arranged by size: 1648 320 129 BbeI G_GCGC'C Cuts at: 0 1774 2097 Size: 1774 323 BbsI GAAGACnn'nnnn_ Cuts at: 0 443 1656 2097 Size: 443 1213 441 Fragments arranged by size: 1213 443 441 BglII A'GATC_T Cuts at: 0 686 1697 2097 Size: 686 1011 400 Fragments arranged by size: 1011 686 400 BlpI GC'TnA_GC Cuts at: 0 1272 2097 Size: 1272 825 Bme1580I G_kGCm'C Cuts at: 0 129 1546 1955 2097 Size: 129 1417 409 142 Fragments arranged by size: 1417 409 142 129 BmrI ACTGGGnnnn_n' Cuts at: 0 751 2097 Size: 751 1346 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 609 2097 Size: 609 1488 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 1569 2097 Size: 184 1385 528 Fragments arranged by size: 1385 528 184 Bpu10I CC'TnA_GC Cuts at: 0 539 2097 Size: 539 1558 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 733 881 1222 1951 2097 Size: 733 148 341 729 146 Fragments arranged by size: 733 729 341 148 146 BsaAI yAC'GTr Cuts at: 0 1176 1902 2097 Size: 1176 726 195 Fragments arranged by size: 1176 726 195 BsaBI GATnn'nnATC Cuts at: 0 1729 2097 Size: 1729 368 BsaHI Gr'CG_yC Cuts at: 0 1154 1610 1771 2097 Size: 1154 456 161 326 Fragments arranged by size: 1154 456 326 161 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 627 657 2097 Size: 627 30 1440 Fragments arranged by size: 1440 627 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 205 635 1564 2097 Size: 205 430 929 533 Fragments arranged by size: 929 533 430 205 BseYI C'CCAG_C Cuts at: 0 496 1117 1253 2097 Size: 496 621 136 844 Fragments arranged by size: 844 621 496 136 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1462 1924 2041 2097 Size: 1462 462 117 56 Fragments arranged by size: 1462 462 117 56 BsiHKAI G_wGCw'C Cuts at: 0 1573 2097 Size: 1573 524 BsmI GAATG_Cn' Cuts at: 0 634 2097 Size: 634 1463 Bsp1286I G_dGCh'C Cuts at: 0 129 449 1546 1573 1955 2097 Size: 129 320 1097 27 382 142 Fragments arranged by size: 1097 382 320 142 129 27 BspHI T'CATG_A Cuts at: 0 1070 2097 Size: 1070 1027 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 376 610 1299 2097 Size: 376 234 689 798 Fragments arranged by size: 798 689 376 234 BsrBI CCG'CTC Cuts at: 0 861 963 2097 Size: 861 102 1134 Fragments arranged by size: 1134 861 102 BsrDI GCAATG_nn' Cuts at: 0 890 2097 Size: 890 1207 BssSI C'ACGA_G Cuts at: 0 1393 2097 Size: 1393 704 BstAPI GCAn_nnn'nTGC Cuts at: 0 2019 2097 Size: 2019 78 BstYI r'GATC_y Cuts at: 0 686 1035 1697 2097 Size: 686 349 662 400 Fragments arranged by size: 686 662 400 349 Bsu36I CC'TnA_GG Cuts at: 0 1463 2097 Size: 1463 634 BtgI C'CryG_G Cuts at: 0 1451 1751 1947 2097 Size: 1451 300 196 150 Fragments arranged by size: 1451 300 196 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 386 1955 2097 Size: 386 1569 142 Fragments arranged by size: 1569 386 142 BtsI GCAGTG_nn' Cuts at: 0 1004 1052 1496 2097 Size: 1004 48 444 601 Fragments arranged by size: 1004 601 444 48 DraIII CAC_nnn'GTG Cuts at: 0 1485 2097 Size: 1485 612 EaeI y'GGCC_r Cuts at: 0 782 1223 1877 2097 Size: 782 441 654 220 Fragments arranged by size: 782 654 441 220 EarI CTCTTCn'nnn_ Cuts at: 0 70 437 1393 1785 2097 Size: 70 367 956 392 312 Fragments arranged by size: 956 392 367 312 70 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 119 184 539 564 1129 1453 1569 2097 Size: 119 65 355 25 565 324 116 528 Fragments arranged by size: 565 528 355 324 119 116 65 25 EcoO109I rG'GnC_Cy Cuts at: 0 590 2097 Size: 590 1507 FspI TGC'GCA Cuts at: 0 1987 2097 Size: 1987 110 HaeII r_GCGC'y Cuts at: 0 996 1774 2097 Size: 996 778 323 Fragments arranged by size: 996 778 323 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 627 659 807 839 1621 1653 1729 1761 Size: 627 32 148 32 782 32 76 32 Cuts at: 1761 2097 Size: 336 Fragments arranged by size: 782 627 336 148 76 32 32 32 32 HincII GTy'rAC Cuts at: 0 170 870 2097 Size: 170 700 1227 Fragments arranged by size: 1227 700 170 KasI G'GCGC_C Cuts at: 0 1770 2097 Size: 1770 327 KpnI G_GTAC'C Cuts at: 0 953 2097 Size: 953 1144 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1706 2097 Size: 1706 391 MscI TGG'CCA Cuts at: 0 784 1225 2097 Size: 784 441 872 Fragments arranged by size: 872 784 441 MslI CAynn'nnrTG Cuts at: 0 1174 1418 1624 1900 1933 2097 Size: 1174 244 206 276 33 164 Fragments arranged by size: 1174 276 244 206 164 33 MspA1I CmG'CkG Cuts at: 0 162 496 505 1151 1476 2035 2097 Size: 162 334 9 646 325 559 62 Fragments arranged by size: 646 559 334 325 162 62 9 NarI GG'CG_CC Cuts at: 0 1771 2097 Size: 1771 326 NdeI CA'TA_TG Cuts at: 0 882 2097 Size: 882 1215 NspI r_CATG'y Cuts at: 0 1092 1137 1533 1623 2097 Size: 1092 45 396 90 474 Fragments arranged by size: 1092 474 396 90 45 PciI A'CATG_T Cuts at: 0 1133 1529 1619 2097 Size: 1133 396 90 478 Fragments arranged by size: 1133 478 396 90 PflMI CCAn_nnn'nTGG Cuts at: 0 680 1872 2097 Size: 680 1192 225 Fragments arranged by size: 1192 680 225 PfoI T'CCnGG_A Cuts at: 0 617 1639 2097 Size: 617 1022 458 Fragments arranged by size: 1022 617 458 PmlI CAC'GTG Cuts at: 0 1176 1902 2097 Size: 1176 726 195 Fragments arranged by size: 1176 726 195 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1120 1152 2097 Size: 1120 32 945 Fragments arranged by size: 1120 945 32 PspOMI G'GGCC_C Cuts at: 0 125 2097 Size: 125 1972 PstI C_TGCA'G Cuts at: 0 495 1270 1678 2097 Size: 495 775 408 419 Fragments arranged by size: 775 495 419 408 PvuII CAG'CTG Cuts at: 0 162 496 505 1476 2035 2097 Size: 162 334 9 971 559 62 Fragments arranged by size: 971 559 334 162 62 9 SapI GCTCTTCn'nnn_ Cuts at: 0 437 2097 Size: 437 1660 ScaI AGT'ACT Cuts at: 0 910 2097 Size: 910 1187 SexAI A'CCwGG_T Cuts at: 0 941 1190 2069 2097 Size: 941 249 879 28 Fragments arranged by size: 941 879 249 28 SfcI C'TryA_G Cuts at: 0 491 1101 1266 1674 2097 Size: 491 610 165 408 423 Fragments arranged by size: 610 491 423 408 165 SfoI GGC'GCC Cuts at: 0 1772 2097 Size: 1772 325 SmlI C'TyrA_G Cuts at: 0 712 896 1237 1966 2097 Size: 712 184 341 729 131 Fragments arranged by size: 729 712 341 184 131 SphI G_CATG'C Cuts at: 0 1092 2097 Size: 1092 1005 StyI C'CwwG_G Cuts at: 0 449 548 1658 2097 Size: 449 99 1110 439 Fragments arranged by size: 1110 449 439 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 187 328 1744 1912 1992 2097 Size: 187 141 1416 168 80 105 Fragments arranged by size: 1416 187 168 141 105 80 TatI w'GTAC_w Cuts at: 0 908 1028 1130 2097 Size: 908 120 102 967 Fragments arranged by size: 967 908 120 102 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1443 1475 2097 Size: 1443 32 622 Fragments arranged by size: 1443 622 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1489 1932 2097 Size: 1489 443 165 Fragments arranged by size: 1489 443 165 Enzymes that do cut: Acc65I AcuI AflIII AhdI AleI AlfI AlwNI ApaI BanI BanII BbeI BbsI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI Eco57MI EcoO109I FspI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NarI NdeI NspI PciI PflMI PfoI PmlI PpiI PspOMI PstI PvuII SapI ScaI SexAI SfcI SfoI SmlI SphI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI BmgBI BmtI BsaI BsaWI BsiEI BsiWI BsmBI BspEI BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HindIII HpaI MfeI MluI NaeI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI