(Circular) (Six-base) MAPSORT of: pFN21AB6574.seq Check: 9175 from: 1 to: 5908 pFN21AB6574 5908 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2468 3718 278 Size: 53 83 186 936 932 1250 2468 Fragments arranged by size: 2468 1250 936 932 186 83 53 AccI GT'mk_AC Cuts at: 1937 2676 3251 1937 Size: 739 575 4594 Fragments arranged by size: 4594 739 575 Acc65I G'GTAC_C Cuts at: 2144 3229 2144 Size: 1085 4823 AclI AA'CG_TT Cuts at: 1563 2072 4035 4408 1563 Size: 509 1963 373 3063 Fragments arranged by size: 3063 1963 509 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2608 2786 3002 3971 5355 5623 1632 Size: 976 178 216 969 1384 268 1917 Fragments arranged by size: 1917 1384 976 969 268 216 178 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5711 AflIII A'CryG_T Cuts at: 1203 4807 5784 1203 Size: 3604 977 1327 Fragments arranged by size: 3604 1327 977 AgeI A'CCGG_T Cuts at: 4739 4739 Size: 5908 AhdI GACnn_n'nnGTC Cuts at: 4637 4637 Size: 5908 AleI CACnn'nnGTG Cuts at: 1166 2232 1166 Size: 1066 4842 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2288 1184 Size: 1104 4804 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2739 2771 2739 Size: 32 5876 AlwNI CAG_nnn'CTG Cuts at: 2764 2980 3651 5223 2764 Size: 216 671 1572 3449 Fragments arranged by size: 3449 1572 671 216 ApaLI G'TGCA_C Cuts at: 3965 5121 3965 Size: 1156 4752 ApoI r'AATT_y Cuts at: 1493 3217 3443 3595 5704 1493 Size: 1724 226 152 2109 1697 Fragments arranged by size: 2109 1724 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4462 160 Size: 872 3430 1606 Fragments arranged by size: 3430 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 AvaI C'yCGr_G Cuts at: 1870 1943 3233 1870 Size: 73 1290 4545 Fragments arranged by size: 4545 1290 73 BamHI G'GATC_C Cuts at: 3238 3605 3238 Size: 367 5541 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2144 2690 3229 4684 618 Size: 325 217 627 357 546 539 1455 1842 Fragments arranged by size: 1842 1455 627 546 539 357 325 217 BanII G_rGCy'C Cuts at: 1391 3227 1391 Size: 1836 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2186 2940 3110 928 Size: 971 287 754 170 3726 Fragments arranged by size: 3726 971 754 287 170 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4099 4133 1903 Size: 34 2162 34 3678 Fragments arranged by size: 3678 2162 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3800 5010 3800 Size: 1210 4698 BclI T'GATC_A Cuts at: 1548 1548 Size: 5908 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2659 4519 136 Size: 107 122 71 631 605 987 1860 1525 Fragments arranged by size: 1860 1525 987 631 605 122 107 71 BglII A'GATC_T Cuts at: 5903 5903 Size: 5908 BlpI GC'TnA_GC Cuts at: 3325 3325 Size: 5908 Bme1580I G_kGCm'C Cuts at: 1790 3969 5125 1790 Size: 2179 1156 2573 Fragments arranged by size: 2573 2179 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5908 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2961 3639 4597 Size: 416 53 83 399 24 1544 678 958 Cuts at: 4597 442 Size: 1753 Fragments arranged by size: 1753 1544 958 678 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5908 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2394 2394 Size: 5908 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4568 1239 Size: 3329 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2363 4033 4898 5196 2363 Size: 1670 865 298 3075 Fragments arranged by size: 3075 1670 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2519 4571 882 Size: 617 1020 2052 2219 Fragments arranged by size: 2219 2052 1020 617 BsaAI yAC'GTr Cuts at: 493 1204 3699 493 Size: 711 2495 2702 Fragments arranged by size: 2702 2495 711 BsaBI GATnn'nnATC Cuts at: 5902 5902 Size: 5908 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2465 3715 4097 4732 Size: 53 83 186 936 932 1250 382 635 Cuts at: 4732 275 Size: 1451 Fragments arranged by size: 1451 1250 936 932 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2561 4341 4739 5013 5160 5571 2561 Size: 1780 398 274 147 411 2898 Fragments arranged by size: 2898 1780 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2062 2092 2261 2291 1178 Size: 30 165 30 659 30 169 30 4795 Fragments arranged by size: 4795 659 169 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2627 1186 Size: 1441 4467 BseYI C'CCAG_C Cuts at: 5111 5607 5111 Size: 496 5412 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2867 1163 Size: 573 1131 4204 Fragments arranged by size: 4204 1131 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3358 4119 5147 1540 Size: 275 186 1357 761 1028 2301 Fragments arranged by size: 2301 1357 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3969 4054 5125 3969 Size: 85 1071 4752 Fragments arranged by size: 4752 1071 85 BsmI GAATG_Cn' Cuts at: 2327 2618 3419 3512 2327 Size: 291 801 93 4723 Fragments arranged by size: 4723 801 291 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2456 2456 Size: 5908 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3227 3969 4054 5125 1391 Size: 399 1437 742 85 1071 2174 Fragments arranged by size: 2174 1437 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2561 5571 2561 Size: 3010 2898 BspHI T'CATG_A Cuts at: 3797 5527 3797 Size: 1730 4178 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3263 844 Size: 2419 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3795 1394 Size: 429 1972 3507 Fragments arranged by size: 3507 1972 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4403 4577 65 Size: 1183 3155 174 1396 Fragments arranged by size: 3155 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4552 4739 1700 Size: 110 2742 187 2869 Fragments arranged by size: 2869 2742 187 110 BsrGI T'GTAC_A Cuts at: 96 2165 3135 96 Size: 2069 970 2869 Fragments arranged by size: 2869 2069 970 BssHII G'CGCG_C Cuts at: 1925 2025 1925 Size: 100 5808 BssSI C'ACGA_G Cuts at: 2030 3968 4980 2030 Size: 1938 1012 2958 Fragments arranged by size: 2958 1938 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5908 BstBI TT'CG_AA Cuts at: 4712 4712 Size: 5908 BstEII G'GTnAC_C Cuts at: 1184 3173 1184 Size: 1989 3919 BstYI r'GATC_y Cuts at: 1972 2976 3238 3605 3990 4007 5459 5545 5557 Size: 1004 262 367 385 17 1452 86 12 Cuts at: 5557 5903 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1004 385 367 346 262 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5356 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5169 Fragments arranged by size: 5169 683 56 BtsI GCAGTG_nn' Cuts at: 2509 3424 4218 4238 2509 Size: 915 794 20 4179 Fragments arranged by size: 4179 915 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5908 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5873 DraI TTT'AAA Cuts at: 3212 3212 Size: 5908 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4915 809 Size: 507 3599 1802 Fragments arranged by size: 3599 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3355 4244 5873 Size: 54 1425 50 275 18 1525 889 1629 Cuts at: 5873 8 Size: 43 Fragments arranged by size: 1629 1525 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3355 1537 Size: 275 1543 4090 Fragments arranged by size: 4090 1543 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3838 1353 Size: 2485 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4495 4869 5015 387 Size: 1064 138 2906 374 146 1280 Fragments arranged by size: 2906 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2608 2786 3002 3971 4568 5355 5623 Size: 393 976 178 216 969 597 787 268 Cuts at: 5623 1239 Size: 1524 Fragments arranged by size: 1524 976 969 787 597 393 268 216 178 EcoNI CCTnn'n_nnAGG Cuts at: 2609 2609 Size: 5908 EcoO109I rG'GnC_Cy Cuts at: 3034 3034 Size: 5908 EcoRI G'AATT_C Cuts at: 3217 3595 3217 Size: 378 5530 FspI TGC'GCA Cuts at: 4414 4414 Size: 5908 HaeII r_GCGC'y Cuts at: 1678 2338 5055 1678 Size: 660 2717 2531 Fragments arranged by size: 2717 2531 660 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2453 2485 4549 4581 4623 Size: 32 135 32 881 32 2064 32 42 Cuts at: 4623 4655 1373 Size: 32 2626 Fragments arranged by size: 2626 2064 881 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3252 3498 669 Size: 988 281 1314 246 3079 Fragments arranged by size: 3079 1314 988 281 246 HindIII A'AGCT_T Cuts at: 748 2905 748 Size: 2157 3751 HpaI GTT'AAC Cuts at: 3498 3498 Size: 5908 KpnI G_GTAC'C Cuts at: 2148 3233 2148 Size: 1085 4823 MfeI C'AATT_G Cuts at: 3507 3507 Size: 5908 MluI A'CGCG_T Cuts at: 5784 5784 Size: 5908 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5022 5206 1305 Size: 3717 184 2007 Fragments arranged by size: 3717 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4086 Fragments arranged by size: 4086 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2232 3866 4225 4384 518 Size: 648 1066 1634 359 159 2042 Fragments arranged by size: 2042 1634 1066 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2119 2223 2761 2851 3324 4001 5149 5394 Size: 513 104 538 90 473 677 1148 245 Cuts at: 5394 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 538 513 473 245 104 90 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5356 NdeI CA'TA_TG Cuts at: 387 2555 387 Size: 2168 3740 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5908 NotI GC'GGCC_GC Cuts at: 3355 3355 Size: 5908 NspI r_CATG'y Cuts at: 1132 1229 2867 2961 3266 4811 1132 Size: 97 1638 94 305 1545 2229 Fragments arranged by size: 2229 1638 1545 305 97 94 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5908 PciI A'CATG_T Cuts at: 4807 4807 Size: 5908 PflMI CCAn_nnn'nTGG Cuts at: 1108 2055 1108 Size: 947 4961 PfoI T'CCnGG_A Cuts at: 2380 2380 Size: 5908 PmeI GTTT'AAAC Cuts at: 3212 3212 Size: 5908 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2336 2368 3977 4009 5490 5522 2336 Size: 32 1609 32 1481 32 2722 Fragments arranged by size: 2722 1609 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2133 2241 1613 Size: 520 108 5280 Fragments arranged by size: 5280 520 108 PsiI TTA'TAA Cuts at: 3478 3478 Size: 5908 PstI C_TGCA'G Cuts at: 830 2255 2277 3260 830 Size: 1425 22 983 3478 Fragments arranged by size: 3478 1425 983 22 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5908 PvuII CAG'CTG Cuts at: 2119 2223 2761 2119 Size: 104 538 5266 Fragments arranged by size: 5266 538 104 SalI G'TCGA_C Cuts at: 1936 3250 1936 Size: 1314 4594 SbfI CC_TGCA'GG Cuts at: 3260 3260 Size: 5908 ScaI AGT'ACT Cuts at: 4156 5868 4156 Size: 1712 4196 SexAI A'CCwGG_T Cuts at: 3177 3177 Size: 5908 SfcI C'TryA_G Cuts at: 826 1045 2251 2273 2482 3256 4391 5072 5263 Size: 219 1206 22 209 774 1135 681 191 Cuts at: 5263 826 Size: 1471 Fragments arranged by size: 1471 1206 1135 774 681 219 209 191 22 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5908 SmaI CCC'GGG Cuts at: 1872 3235 1872 Size: 1363 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2342 4012 4913 5175 820 Size: 197 926 399 1670 901 262 1553 Fragments arranged by size: 1670 1553 926 901 399 262 197 SnaBI TAC'GTA Cuts at: 493 3699 493 Size: 3206 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5908 SphI G_CATG'C Cuts at: 1132 2961 3266 1132 Size: 1829 305 3774 Fragments arranged by size: 3774 1829 305 SspI AAT'ATT Cuts at: 5 52 3832 5 Size: 47 3780 2081 Fragments arranged by size: 3780 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5908 StyI C'CwwG_G Cuts at: 513 1065 3347 513 Size: 552 2282 3074 Fragments arranged by size: 3074 2282 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2078 2318 3935 3952 4105 4290 4735 Size: 1255 498 240 1617 17 153 185 445 Cuts at: 4735 325 Size: 1498 Fragments arranged by size: 1617 1498 1255 498 445 240 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2165 3135 4154 Size: 274 80 33 51 1443 188 970 1019 Cuts at: 4154 5866 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1019 970 274 188 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2376 2408 2907 2939 5868 5900 2376 Size: 32 499 32 2929 32 2384 Fragments arranged by size: 2929 2384 499 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5908 XbaI T'CTAG_A Cuts at: 3244 3244 Size: 5908 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5908 XmaI C'CCGG_G Cuts at: 1870 3233 1870 Size: 1363 4545 XmnI GAAnn'nnTTC Cuts at: 2073 4037 2073 Size: 1964 3944 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2466 3716 276 Size: 53 83 186 936 932 1250 2468 Fragments arranged by size: 2468 1250 936 932 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI