(Circular) (Six-base) MAPSORT of: pFN21AE4586.seq Check: 3697 from: 1 to: 7375 pFN21AE4586 7375 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2575 2575 Size: 7375 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4398 5185 278 Size: 53 83 186 936 2862 787 2468 Fragments arranged by size: 2862 2468 936 787 186 83 53 AccI GT'mk_AC Cuts at: 1937 3531 3981 4718 1937 Size: 1594 450 737 4594 Fragments arranged by size: 4594 1594 737 450 Acc65I G'GTAC_C Cuts at: 4696 4696 Size: 7375 AclI AA'CG_TT Cuts at: 1563 5502 5875 1563 Size: 3939 373 3063 Fragments arranged by size: 3939 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2882 3467 5438 6822 7090 1632 Size: 1250 585 1971 1384 268 1917 Fragments arranged by size: 1971 1917 1384 1250 585 268 AflII C'TTAA_G Cuts at: 820 1017 4274 820 Size: 197 3257 3921 Fragments arranged by size: 3921 3257 197 AflIII A'CryG_T Cuts at: 1203 6274 7251 1203 Size: 5071 977 1327 Fragments arranged by size: 5071 1327 977 AgeI A'CCGG_T Cuts at: 6206 6206 Size: 7375 AhdI GACnn_n'nnGTC Cuts at: 6104 6104 Size: 7375 AleI CACnn'nnGTG Cuts at: 1166 2381 1166 Size: 1215 6160 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4029 4631 1184 Size: 2845 602 3928 Fragments arranged by size: 3928 2845 602 AlwNI CAG_nnn'CTG Cuts at: 5118 6690 5118 Size: 1572 5803 ApaI G_GGCC'C Cuts at: 2033 3379 2033 Size: 1346 6029 ApaLI G'TGCA_C Cuts at: 5432 6588 5432 Size: 1156 6219 ApoI r'AATT_y Cuts at: 1493 3571 4684 4910 5062 7171 1493 Size: 2078 1113 226 152 2109 1697 Fragments arranged by size: 2109 2078 1697 1113 226 152 AseI AT'TA_AT Cuts at: 160 1032 5929 160 Size: 872 4897 1606 Fragments arranged by size: 4897 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7375 AvaI C'yCGr_G Cuts at: 1870 1943 2127 4700 1870 Size: 73 184 2573 4545 Fragments arranged by size: 4545 2573 184 73 BamHI G'GATC_C Cuts at: 4705 5072 4705 Size: 367 7008 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2231 3581 4622 4696 6151 Size: 325 217 627 444 1350 1041 74 1455 Cuts at: 6151 618 Size: 1842 Fragments arranged by size: 1842 1455 1350 1041 627 444 325 217 74 BanII G_rGCy'C Cuts at: 1391 2033 2840 3162 3216 3363 3379 3633 3786 Size: 642 807 322 54 147 16 254 153 Cuts at: 3786 3896 4266 4530 4694 1391 Size: 110 370 264 164 4072 Fragments arranged by size: 4072 807 642 370 322 264 254 164 153 147 110 54 16 BbeI G_GCGC'C Cuts at: 4626 4626 Size: 7375 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3232 4513 928 Size: 971 1333 1281 3790 Fragments arranged by size: 3790 1333 1281 971 BbvCI CC'TCA_GC Cuts at: 3566 3566 Size: 7375 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2399 2433 4453 4487 5566 5600 1903 Size: 34 462 34 2020 34 1079 34 3678 Fragments arranged by size: 3678 2020 1079 462 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5267 6477 5267 Size: 1210 6165 BclI T'GATC_A Cuts at: 1548 1548 Size: 7375 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2930 3734 4209 Size: 107 122 71 631 605 1258 804 475 Cuts at: 4209 4503 5986 136 Size: 294 1483 1525 Fragments arranged by size: 1525 1483 1258 804 631 605 475 294 122 107 71 BglII A'GATC_T Cuts at: 7370 7370 Size: 7375 BlpI GC'TnA_GC Cuts at: 4448 4792 4448 Size: 344 7031 Bme1580I G_kGCm'C Cuts at: 1790 2033 2664 3379 3584 3603 3825 5436 6592 Size: 243 631 715 205 19 222 1611 1156 Cuts at: 6592 1790 Size: 2573 Fragments arranged by size: 2573 1611 1156 715 631 243 222 205 19 BmgBI CAC'GTC Cuts at: 1318 2472 3774 1318 Size: 1154 1302 4919 Fragments arranged by size: 4919 1302 1154 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5106 6064 442 Size: 416 53 83 399 24 3689 958 1753 Fragments arranged by size: 3689 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7375 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 2540 2627 2714 2911 3230 3671 3800 Size: 1061 240 87 87 197 319 441 129 Cuts at: 3800 4103 4511 6035 1239 Size: 303 408 1524 2579 Fragments arranged by size: 2579 1524 1061 441 408 319 303 240 197 129 87 87 Bpu10I CC'TnA_GC Cuts at: 3338 3344 3566 3338 Size: 6 222 7147 Fragments arranged by size: 7147 222 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3074 4514 5500 6365 6663 3074 Size: 1440 986 865 298 3786 Fragments arranged by size: 3786 1440 986 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2504 4037 6038 882 Size: 617 1005 1533 2001 2219 Fragments arranged by size: 2219 2001 1533 1005 617 BsaAI yAC'GTr Cuts at: 493 1204 4101 4585 5166 493 Size: 711 2897 484 581 2702 Fragments arranged by size: 2897 2702 711 581 484 BsaBI GATnn'nnATC Cuts at: 7369 7369 Size: 7375 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2775 4395 4623 5182 Size: 53 83 186 936 1242 1620 228 559 Cuts at: 5182 5564 6199 275 Size: 382 635 1451 Fragments arranged by size: 1620 1451 1242 936 635 559 382 228 186 83 53 BsaWI w'CCGG_w Cuts at: 5808 6206 6480 6627 7038 5808 Size: 398 274 147 411 6145 Fragments arranged by size: 6145 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2909 2939 1178 Size: 30 165 30 1506 30 5614 Fragments arranged by size: 5614 1506 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2128 2494 4271 4274 4400 1186 Size: 942 366 1777 3 126 4161 Fragments arranged by size: 4161 1777 942 366 126 3 BseYI C'CCAG_C Cuts at: 2026 2199 2783 4560 6578 7074 2026 Size: 173 584 1777 2018 496 2327 Fragments arranged by size: 2327 2018 1777 584 496 173 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2359 2867 3067 1163 Size: 573 623 508 200 5471 Fragments arranged by size: 5471 623 573 508 200 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4111 4254 4318 4674 4825 5586 Size: 275 186 2110 143 64 356 151 761 Cuts at: 5586 6614 1540 Size: 1028 2301 Fragments arranged by size: 2301 2110 1028 761 356 275 186 151 143 64 BsiHKAI G_wGCw'C Cuts at: 2134 2634 3350 3633 3786 3896 4266 4530 4599 Size: 500 716 283 153 110 370 264 69 Cuts at: 4599 5436 5521 6592 2134 Size: 837 85 1071 2917 Fragments arranged by size: 2917 1071 837 716 500 370 283 264 153 110 85 69 BsmI GAATG_Cn' Cuts at: 4886 4979 4886 Size: 93 7282 BsmBI CGTCTCn'nnnn_ Cuts at: 2163 3780 2163 Size: 1617 5758 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2134 2634 2664 2840 3162 3216 Size: 399 243 101 500 30 176 322 54 Cuts at: 3216 3350 3363 3379 3584 3603 3633 3786 3825 Size: 134 13 16 205 19 30 153 39 Cuts at: 3825 3896 4266 4530 4599 4694 5436 5521 6592 Size: 71 370 264 69 95 742 85 1071 Cuts at: 6592 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 500 399 370 322 264 243 205 176 153 134 101 95 85 71 69 54 39 30 30 19 16 13 BspEI T'CCGG_A Cuts at: 7038 7038 Size: 7375 BspHI T'CATG_A Cuts at: 5264 6994 5264 Size: 1730 5645 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2343 2575 2856 3150 3543 4730 844 Size: 1499 232 281 294 393 1187 3489 Fragments arranged by size: 3489 1499 1187 393 294 281 232 BsrBI CCG'CTC Cuts at: 1394 1823 3963 5262 1394 Size: 429 2140 1299 3507 Fragments arranged by size: 3507 2140 1299 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5870 6044 65 Size: 1183 4622 174 1396 Fragments arranged by size: 4622 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2345 2442 3311 3398 3659 3728 4106 Size: 110 535 97 869 87 261 69 378 Cuts at: 4106 6019 6206 1700 Size: 1913 187 2869 Fragments arranged by size: 2869 1913 869 535 378 261 187 110 97 87 69 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7375 BssHII G'CGCG_C Cuts at: 1925 4055 1925 Size: 2130 5245 BssSI C'ACGA_G Cuts at: 5435 6447 5435 Size: 1012 6363 BstAPI GCAn_nnn'nTGC Cuts at: 794 2335 3279 3288 3588 794 Size: 1541 944 9 300 4581 Fragments arranged by size: 4581 1541 944 300 9 BstBI TT'CG_AA Cuts at: 6179 6179 Size: 7375 BstEII G'GTnAC_C Cuts at: 1184 3391 1184 Size: 2207 5168 BstXI CCAn_nnnn'nTGG Cuts at: 3664 3724 4035 3664 Size: 60 311 7004 Fragments arranged by size: 7004 311 60 BstYI r'GATC_y Cuts at: 1972 4065 4705 5072 5457 5474 6926 7012 7024 Size: 2093 640 367 385 17 1452 86 12 Cuts at: 7024 7370 1972 Size: 346 1977 Fragments arranged by size: 2093 1977 1452 640 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2841 2841 Size: 7375 BtgI C'CryG_G Cuts at: 513 1065 2004 2592 3072 3636 3804 3897 4248 Size: 552 939 588 480 564 168 93 351 Cuts at: 4248 4338 513 Size: 90 3550 Fragments arranged by size: 3550 939 588 564 552 480 351 168 93 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2713 3250 487 Size: 683 56 1487 537 4612 Fragments arranged by size: 4612 1487 683 537 56 BtsI GCAGTG_nn' Cuts at: 2016 2852 4891 5685 5705 2016 Size: 836 2039 794 20 3686 Fragments arranged by size: 3686 2039 836 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7375 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7340 DraI TTT'AAA Cuts at: 4679 4679 Size: 7375 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2393 6382 809 Size: 507 1077 3989 1802 Fragments arranged by size: 3989 1802 1077 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2343 2589 2617 Size: 54 1425 50 275 18 513 246 28 Cuts at: 2617 3381 3457 3484 3726 3927 4108 4251 4284 Size: 764 76 27 242 201 181 143 33 Cuts at: 4284 4671 4822 5711 7340 8 Size: 387 151 889 1629 43 Fragments arranged by size: 1629 1425 889 764 513 387 275 246 242 201 181 151 143 76 54 50 43 33 28 27 18 EagI C'GGCC_G Cuts at: 1537 1812 4108 4251 4671 4822 1537 Size: 275 2296 143 420 151 4090 Fragments arranged by size: 4090 2296 420 275 151 143 EarI CTCTTCn'nnn_ Cuts at: 1353 2455 2828 3551 3704 5305 1353 Size: 1102 373 723 153 1601 3423 Fragments arranged by size: 3423 1601 1102 723 373 153 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2468 2612 5962 6336 6482 387 Size: 1064 138 879 144 3350 374 146 1280 Fragments arranged by size: 3350 1280 1064 879 374 146 144 138 EcoICRI GAG'CTC Cuts at: 3631 3784 3894 4264 4528 3631 Size: 153 110 370 264 6478 Fragments arranged by size: 6478 370 264 153 110 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 2540 2627 2714 2882 2911 3230 Size: 393 668 240 87 87 168 29 319 Cuts at: 3230 3467 3671 3800 4103 4511 5438 6035 6822 Size: 237 204 129 303 408 927 597 787 Cuts at: 6822 7090 1239 Size: 268 1524 Fragments arranged by size: 1524 927 787 668 597 408 393 319 303 268 240 237 204 168 129 87 87 29 EcoNI CCTnn'n_nnAGG Cuts at: 4457 4457 Size: 7375 EcoO109I rG'GnC_Cy Cuts at: 2030 2124 2299 2654 2845 3375 3376 3451 2030 Size: 94 175 355 191 530 1 75 5954 Fragments arranged by size: 5954 530 355 191 175 94 75 1 EcoRI G'AATT_C Cuts at: 4684 5062 4684 Size: 378 6997 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3871 4654 3871 Size: 783 6592 FseI GG_CCGG'CC Cuts at: 3732 3732 Size: 7375 FspI TGC'GCA Cuts at: 2534 5881 2534 Size: 3347 4028 HaeII r_GCGC'y Cuts at: 1678 2701 4152 4365 4626 6522 1678 Size: 1023 1451 213 261 1896 2531 Fragments arranged by size: 2531 1896 1451 1023 261 213 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2909 2941 6016 6048 6090 Size: 32 135 32 1337 32 3075 32 42 Cuts at: 6090 6122 1373 Size: 32 2626 Fragments arranged by size: 3075 2626 1337 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2626 3190 4719 4965 669 Size: 988 281 688 564 1529 246 3079 Fragments arranged by size: 3079 1529 988 688 564 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7375 HpaI GTT'AAC Cuts at: 4965 4965 Size: 7375 KasI G'GCGC_C Cuts at: 4622 4622 Size: 7375 KpnI G_GTAC'C Cuts at: 4700 4700 Size: 7375 MfeI C'AATT_G Cuts at: 4974 4974 Size: 7375 MluI A'CGCG_T Cuts at: 7251 7251 Size: 7375 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2037 2082 6489 6673 1305 Size: 732 45 4407 184 2007 Fragments arranged by size: 4407 2007 732 184 45 MscI TGG'CCA Cuts at: 10 64 1489 1832 3383 3459 4286 10 Size: 54 1425 343 1551 76 827 3099 Fragments arranged by size: 3099 1551 1425 827 343 76 54 MslI CAynn'nnrTG Cuts at: 518 1166 2381 3071 3473 3722 3914 4166 5333 Size: 648 1215 690 402 249 192 252 1167 Cuts at: 5333 5692 5851 518 Size: 359 159 2042 Fragments arranged by size: 2042 1215 1167 690 648 402 359 252 249 192 159 MspA1I CmG'CkG Cuts at: 1606 2026 2177 2186 2373 2381 3043 3565 4359 Size: 420 151 9 187 8 662 522 794 Cuts at: 4359 4542 4791 5468 6616 6861 1606 Size: 183 249 677 1148 245 2120 Fragments arranged by size: 2120 1148 794 677 662 522 420 249 245 187 183 151 9 8 NaeI GCC'GGC Cuts at: 2347 3400 3730 2347 Size: 1053 330 5992 Fragments arranged by size: 5992 1053 330 NarI GG'CG_CC Cuts at: 4623 4623 Size: 7375 NcoI C'CATG_G Cuts at: 513 1065 2004 3072 3897 513 Size: 552 939 1068 825 3991 Fragments arranged by size: 3991 1068 939 825 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7375 NgoMIV G'CCGG_C Cuts at: 2345 3398 3728 2345 Size: 1053 330 5992 Fragments arranged by size: 5992 1053 330 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7375 NotI GC'GGCC_GC Cuts at: 4822 4822 Size: 7375 NruI TCG'CGA Cuts at: 2421 2421 Size: 7375 NspI r_CATG'y Cuts at: 1132 1229 2692 2941 4171 4348 4733 6278 1132 Size: 97 1463 249 1230 177 385 1545 2229 Fragments arranged by size: 2229 1545 1463 1230 385 249 177 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7375 PciI A'CATG_T Cuts at: 6274 6274 Size: 7375 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7375 PfoI T'CCnGG_A Cuts at: 2277 3099 4488 2277 Size: 822 1389 5164 Fragments arranged by size: 5164 1389 822 PmeI GTTT'AAAC Cuts at: 4679 4679 Size: 7375 PmlI CAC'GTG Cuts at: 4101 4585 4101 Size: 484 6891 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5444 5476 6957 6989 5444 Size: 32 1481 32 5830 Fragments arranged by size: 5830 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2299 2845 3451 2299 Size: 546 606 6223 Fragments arranged by size: 6223 606 546 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7375 PsiI TTA'TAA Cuts at: 4945 4945 Size: 7375 PspOMI G'GGCC_C Cuts at: 2029 3375 2029 Size: 1346 6029 PstI C_TGCA'G Cuts at: 830 2340 2676 2802 3756 4076 4232 4727 830 Size: 1510 336 126 954 320 156 495 3478 Fragments arranged by size: 3478 1510 954 495 336 320 156 126 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7375 PvuII CAG'CTG Cuts at: 2373 3565 4542 2373 Size: 1192 977 5206 Fragments arranged by size: 5206 1192 977 SacI G_AGCT'C Cuts at: 3633 3786 3896 4266 4530 3633 Size: 153 110 370 264 6478 Fragments arranged by size: 6478 370 264 153 110 SalI G'TCGA_C Cuts at: 1936 4717 1936 Size: 2781 4594 SanDI GG'GwC_CC Cuts at: 2299 2299 Size: 7375 SapI GCTCTTCn'nnn_ Cuts at: 2828 2828 Size: 7375 SbfI CC_TGCA'GG Cuts at: 2802 4727 2802 Size: 1925 5450 ScaI AGT'ACT Cuts at: 5623 7335 5623 Size: 1712 5663 SexAI A'CCwGG_T Cuts at: 3489 3606 3489 Size: 117 7258 SfcI C'TryA_G Cuts at: 826 1045 2336 2672 2798 3752 3814 4072 4228 Size: 219 1291 336 126 954 62 258 156 Cuts at: 4228 4723 5858 6539 6730 826 Size: 495 1135 681 191 1471 Fragments arranged by size: 1471 1291 1135 954 681 495 336 258 219 191 156 126 62 SfiI GGCCn_nnn'nGGCC Cuts at: 3734 3734 Size: 7375 SfoI GGC'GCC Cuts at: 4624 4624 Size: 7375 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7375 SgrAI Cr'CCGG_yG Cuts at: 1700 2442 1700 Size: 742 6633 SmaI CCC'GGG Cuts at: 1872 4702 1872 Size: 2830 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3089 4274 4529 5479 6380 6642 Size: 197 926 1146 1185 255 950 901 262 Cuts at: 6642 820 Size: 1553 Fragments arranged by size: 1553 1185 1146 950 926 901 262 255 197 SnaBI TAC'GTA Cuts at: 493 5166 493 Size: 4673 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7375 SphI G_CATG'C Cuts at: 1132 2692 2941 4348 4733 1132 Size: 1560 249 1407 385 3774 Fragments arranged by size: 3774 1560 1407 385 249 SspI AAT'ATT Cuts at: 5 52 5299 5 Size: 47 5247 2081 Fragments arranged by size: 5247 2081 47 StuI AGG'CCT Cuts at: 1517 3113 4217 1517 Size: 1596 1104 4675 Fragments arranged by size: 4675 1596 1104 StyI C'CwwG_G Cuts at: 513 1065 2004 2289 2304 2769 3072 3454 3846 Size: 552 939 285 15 465 303 382 392 Cuts at: 3846 3897 4401 4814 513 Size: 51 504 413 3074 Fragments arranged by size: 3074 939 552 504 465 413 392 382 303 285 51 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2528 3365 4076 4585 5402 5419 5572 Size: 1255 948 837 711 509 817 17 153 Cuts at: 5572 5757 6202 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 948 837 817 711 509 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2553 5621 7333 Size: 274 80 33 51 1443 576 3068 1712 Cuts at: 7333 96 Size: 138 Fragments arranged by size: 3068 1712 1443 576 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2933 2965 7335 7367 2933 Size: 32 4370 32 2941 Fragments arranged by size: 4370 2941 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4394 1316 Size: 3078 4297 XbaI T'CTAG_A Cuts at: 4711 4711 Size: 7375 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2098 2150 3169 3859 2098 Size: 52 1019 690 5614 Fragments arranged by size: 5614 1019 690 52 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7375 XmaI C'CCGG_G Cuts at: 1870 4700 1870 Size: 2830 4545 XmnI GAAnn'nnTTC Cuts at: 5504 5504 Size: 7375 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4396 5183 276 Size: 53 83 186 936 2862 787 2468 Fragments arranged by size: 2862 2468 936 787 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FspAI NsiI PacI PspXI PsrI RsrII SacII SrfI SwaI