Restriction map for
pFN21AE2543
(Circular) (Six-base) MAPSORT of: pFN21AE2543.seq Check: 8512 from: 1 to: 5500
pFN21AE2543 5500 bp
With 182 enzymes: SgfI *
September 30, 2014 11:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3310 278
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2843 1937
Size: 906 4594
Acc65I G'GTAC_C
Cuts at: 2821 2821
Size: 5500
AclI AA'CG_TT
Cuts at: 1563 3627 4000 1563
Size: 2064 373 3063
Fragments arranged by size:
3063 2064 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 2576 3563 4947 5215 1632
Size: 422 522 987 1384 268 1917
Fragments arranged by size:
1917 1384 987 522 422 268
AfeI AGC'GCT
Cuts at: 2322 2322
Size: 5500
AflII C'TTAA_G
Cuts at: 820 1017 2116 820
Size: 197 1099 4204
Fragments arranged by size:
4204 1099 197
AflIII A'CryG_T
Cuts at: 1203 4399 5376 1203
Size: 3196 977 1327
Fragments arranged by size:
3196 1327 977
AgeI A'CCGG_T
Cuts at: 4331 4331
Size: 5500
AhdI GACnn_n'nnGTC
Cuts at: 4229 4229
Size: 5500
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5500
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5500
AlwNI CAG_nnn'CTG
Cuts at: 3243 4815 3243
Size: 1572 3928
ApaLI G'TGCA_C
Cuts at: 3557 4713 3557
Size: 1156 4344
ApoI r'AATT_y
Cuts at: 1493 2809 3035 3187 5296 1493
Size: 1316 226 152 2109 1697
Fragments arranged by size:
2109 1697 1316 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2510 4054 160
Size: 872 1478 1544 1606
Fragments arranged by size:
1606 1544 1478 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
AvaI C'yCGr_G
Cuts at: 1870 1943 2097 2825 1870
Size: 73 154 728 4545
Fragments arranged by size:
4545 728 154 73
BamHI G'GATC_C
Cuts at: 2830 3197 2830
Size: 367 5133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2821 4276 618
Size: 325 217 627 1034 1455 1842
Fragments arranged by size:
1842 1455 1034 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2819 1391
Size: 1428 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3691 3725 1903
Size: 34 1754 34 3678
Fragments arranged by size:
3678 1754 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3392 4602 3392
Size: 1210 4290
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5500
BfrBI ATG'CAT
Cuts at: 2565 2565
Size: 5500
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4111 136
Size: 107 122 71 631 605 337 2102 1525
Fragments arranged by size:
2102 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5495 5495
Size: 5500
BlpI GC'TnA_GC
Cuts at: 2917 2917
Size: 5500
Bme1580I G_kGCm'C
Cuts at: 1790 3561 4717 1790
Size: 1771 1156 2573
Fragments arranged by size:
2573 1771 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5500
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2243 3231 4189
Size: 416 53 83 399 24 826 988 958
Cuts at: 4189 442
Size: 1753
Fragments arranged by size:
1753 988 958 826 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5500
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2144 2144
Size: 5500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2329 2339 4160 1239
Size: 1090 10 1821 2579
Fragments arranged by size:
2579 1821 1090 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3625 4490 4788 3625
Size: 865 298 4337
Fragments arranged by size:
4337 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4163 882
Size: 617 2664 2219
Fragments arranged by size:
2664 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3291 493
Size: 711 2087 2702
Fragments arranged by size:
2702 2087 711
BsaBI GATnn'nnATC
Cuts at: 5494 5494
Size: 5500
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3307 3689 4324 275
Size: 53 83 186 936 1774 382 635 1451
Fragments arranged by size:
1774 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3933 4331 4605 4752 5163 3933
Size: 398 274 147 411 4270
Fragments arranged by size:
4270 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5275
Fragments arranged by size:
5275 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 1186
Size: 901 4599
BseYI C'CCAG_C
Cuts at: 4703 5199 4703
Size: 496 5004
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4927
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 2020 2026 2950 3711 4739
Size: 275 186 15 4 6 924 761 1028
Cuts at: 4739 1540
Size: 2301
Fragments arranged by size:
2301 1028 924 761 275 186 15 6
4
BsiHKAI G_wGCw'C
Cuts at: 3561 3646 4717 3561
Size: 85 1071 4344
Fragments arranged by size:
4344 1071 85
BsmI GAATG_Cn'
Cuts at: 2479 3011 3104 2479
Size: 532 93 4875
Fragments arranged by size:
4875 532 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2819 3561 3646 4717 1391
Size: 399 1029 742 85 1071 2174
Fragments arranged by size:
2174 1071 1029 742 399 85
BspEI T'CCGG_A
Cuts at: 5163 5163
Size: 5500
BspHI T'CATG_A
Cuts at: 3389 5119 3389
Size: 1730 3770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2855 844
Size: 2011 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 3387 1394
Size: 429 205 1359 3507
Fragments arranged by size:
3507 1359 429 205
BsrDI GCAATG_nn'
Cuts at: 65 1248 2565 3995 4169 65
Size: 1183 1317 1430 174 1396
Fragments arranged by size:
1430 1396 1317 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4144 4331 1700
Size: 110 2334 187 2869
Fragments arranged by size:
2869 2334 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5500
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5500
BssSI C'ACGA_G
Cuts at: 3560 4572 3560
Size: 1012 4488
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2567 794
Size: 1773 3727
BstBI TT'CG_AA
Cuts at: 4304 4304
Size: 5500
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5500
BstYI r'GATC_y
Cuts at: 1972 2089 2830 3197 3582 3599 5051 5137 5149
Size: 117 741 367 385 17 1452 86 12
Cuts at: 5149 5495 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 741 385 367 346 117 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2020 513
Size: 552 939 16 3993
Fragments arranged by size:
3993 939 552 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4761
Fragments arranged by size:
4761 683 56
BtsI GCAGTG_nn'
Cuts at: 3016 3810 3830 3016
Size: 794 20 4686
Fragments arranged by size:
4686 794 20
ClaI AT'CG_AT
Cuts at: 1554 2652 1554
Size: 1098 4402
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5465
DraI TTT'AAA
Cuts at: 2788 2804 2788
Size: 16 5484
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2552 4507 809
Size: 507 1236 1955 1802
Fragments arranged by size:
1955 1802 1236 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2017 2023 2139
Size: 54 1425 50 275 18 187 6 116
Cuts at: 2139 2215 2947 3836 5465 8
Size: 76 732 889 1629 43
Fragments arranged by size:
1629 1425 889 732 275 187 116 76
54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2017 2023 2947 1537
Size: 275 205 6 924 4090
Fragments arranged by size:
4090 924 275 205 6
EarI CTCTTCn'nnn_
Cuts at: 1353 3430 1353
Size: 2077 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2071 4087 4461 4607 387
Size: 1064 138 482 2016 374 146 1280
Fragments arranged by size:
2016 1280 1064 482 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2329 2339 2576 3563 4160 4947
Size: 393 422 275 10 237 987 597 787
Cuts at: 4947 5215 1239
Size: 268 1524
Fragments arranged by size:
1524 987 787 597 422 393 275 268
237 10
EcoO109I rG'GnC_Cy
Cuts at: 2680 2680
Size: 5500
EcoRI G'AATT_C
Cuts at: 2809 3187 2809
Size: 378 5122
EcoRV GAT'ATC
Cuts at: 2254 2330 2254
Size: 76 5424
FspI TGC'GCA
Cuts at: 4006 4006
Size: 5500
HaeII r_GCGC'y
Cuts at: 1678 2324 4647 1678
Size: 646 2323 2531
Fragments arranged by size:
2531 2323 646
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2319 2351 4141 4173 4215
Size: 32 135 32 747 32 1790 32 42
Cuts at: 4215 4247 1373
Size: 32 2626
Fragments arranged by size:
2626 1790 747 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2844 3090 669
Size: 988 281 906 246 3079
Fragments arranged by size:
3079 988 906 281 246
HindIII A'AGCT_T
Cuts at: 748 2198 748
Size: 1450 4050
HpaI GTT'AAC
Cuts at: 3090 3090
Size: 5500
KpnI G_GTAC'C
Cuts at: 2825 2825
Size: 5500
MfeI C'AATT_G
Cuts at: 3099 3099
Size: 5500
MluI A'CGCG_T
Cuts at: 5376 5376
Size: 5500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2500 4614 4798 1305
Size: 1195 2114 184 2007
Fragments arranged by size:
2114 2007 1195 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2141 2217 10
Size: 54 1425 343 309 76 3293
Fragments arranged by size:
3293 1425 343 309 76 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3458 3817 3976 518
Size: 648 2292 359 159 2042
Fragments arranged by size:
2292 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2067 2086 2916 3593 4741 4986 1606
Size: 416 45 19 830 677 1148 245 2120
Fragments arranged by size:
2120 1148 830 677 416 245 45 19
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4009
Fragments arranged by size:
4009 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5500
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5500
NotI GC'GGCC_GC
Cuts at: 2023 2947 2023
Size: 924 4576
NsiI A_TGCA'T
Cuts at: 2567 2567
Size: 5500
NspI r_CATG'y
Cuts at: 1132 1229 2565 2858 4403 1132
Size: 97 1336 293 1545 2229
Fragments arranged by size:
2229 1545 1336 293 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5500
PciI A'CATG_T
Cuts at: 4399 4399
Size: 5500
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2137 1108
Size: 1029 4471
PmeI GTTT'AAAC
Cuts at: 2804 2804
Size: 5500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 5082 5114 3569
Size: 32 1481 32 3955
Fragments arranged by size:
3955 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5500
PsiI TTA'TAA
Cuts at: 3070 3070
Size: 5500
PstI C_TGCA'G
Cuts at: 830 2852 830
Size: 2022 3478
PvuI CG_AT'CG
Cuts at: 2001 2016 2001
Size: 15 5485
SacII CC_GC'GG
Cuts at: 2023 2023
Size: 5500
SalI G'TCGA_C
Cuts at: 1936 2842 1936
Size: 906 4594
SbfI CC_TGCA'GG
Cuts at: 2852 2852
Size: 5500
ScaI AGT'ACT
Cuts at: 3748 5460 3748
Size: 1712 3788
SfcI C'TryA_G
Cuts at: 826 1045 2848 3983 4664 4855 826
Size: 219 1803 1135 681 191 1471
Fragments arranged by size:
1803 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5500
SmaI CCC'GGG
Cuts at: 1872 2827 1872
Size: 955 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2116 3604 4505 4767 820
Size: 197 926 173 1488 901 262 1553
Fragments arranged by size:
1553 1488 926 901 262 197 173
SnaBI TAC'GTA
Cuts at: 493 3291 493
Size: 2798 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5500
SphI G_CATG'C
Cuts at: 1132 2565 2858 1132
Size: 1433 293 3774
Fragments arranged by size:
3774 1433 293
SspI AAT'ATT
Cuts at: 5 52 2772 2793 3424 5
Size: 47 2720 21 631 2081
Fragments arranged by size:
2720 2081 631 47 21
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5500
StyI C'CwwG_G
Cuts at: 513 1065 2004 2939 513
Size: 552 939 935 3074
Fragments arranged by size:
3074 939 935 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3527 3544 3697 3882 4327 325
Size: 1255 1947 17 153 185 445 1498
Fragments arranged by size:
1947 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3746 5458 96
Size: 274 80 33 51 1443 1769 1712 138
Fragments arranged by size:
1769 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5460 5492 5460
Size: 32 5468
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5500
XbaI T'CTAG_A
Cuts at: 2836 2836
Size: 5500
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5500
XmaI C'CCGG_G
Cuts at: 1870 2825 1870
Size: 955 4545
XmnI GAAnn'nnTTC
Cuts at: 3629 3629
Size: 5500
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3308 276
Size: 53 83 186 936 1774 2468
Fragments arranged by size:
2468 1774 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI