################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsgI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MflI MluI MluNI MscI MseI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I TspRI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccIII 428 TCCGGA T^CCGGA AvaI 130 CCCGAG C^[CT]CG[GA]G BanI 315 GGCACC G^G[CT][GA]CC BanII 152 GAGCCC G[GA]GC[CT]^C BcnI 393 CCCGG CC^[GC]GG BmyI 152 GAGCCC G[AGT]GC[ACT]^C BsaWI 428 TCCGGA [AT]^CCGG[AT] BseAI 428 TCCGGA T^CCGGA BseRI 415 CTCCTC GAGGAG(10/8) BsiEI 395 CGGCCG CG[GA][CT]^CG BsoBI 130 CCCGAG C^[CT]CG[GA]G Bsp1286I 152 GAGCCC G[AGT]GC[ACT]^C BspEI 428 TCCGGA T^CCGGA BspHI 222 TCATGA T^CATGA BspMI 72 GCAGGT ACCTGC(4/8) BsrBI 112 CCGCTC CCGCTC(-3/-3) BsrI 21 ACTGG ACTGG(1/-1) DdeI 307 CTCAG C^T[ACGT]AG DpnII 78 GATC ^GATC DraII 91 AGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 442 CCGTGG C^C[GA][CT]GG EaeI 395 CGGCCG [CT]^GGCC[GA] EagI 395 CGGCCG C^GGCCG EclXI 395 CGGCCG C^GGCCG Eco52I 395 CGGCCG C^GGCCG EcoO109I 91 AGGGCCT [GA]G^G[ACGT]CC[CT] FauI 180 CCCGC CCCGC(4/6) HaeII 357 GGCGCT [GA]GCGC^[CT] HinfI 44 GAGTC G^A[ACGT]TC MaeIII 31 GTCAC ^GT[ACGT]AC MboI 78 GATC ^GATC MboII 215 TCTTC GAAGA(8/7) MroI 428 TCCGGA T^CCGGA NaeI 208 GCCGGC GCC^GGC NciI 393 CCCGG CC^[GC]GG NdeII 78 GATC ^GATC NgoMIV 208 GCCGGC G^CCGGC NlaIII 223 CATG CATG^ NlaIV 315 GGCACC GG[ACGT]^[ACGT]CC PflMI 194 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 44 GAGTC GAGTC(4/5) RcaI 222 TCATGA T^CATGA Sau3AI 78 GATC ^GATC SbfI 69 CCTGCAGG CCTGCA^GG ScaI 173 AGTACT AGT^ACT SfaNI 212 GCATC GCATC(5/9) Sse8387I 69 CCTGCAGG CCTGCA^GG TaqI 137 TCGA T^CGA Tsp45I 31 GTCAC ^GT[GC]AC TthHB8I 137 TCGA T^CGA Van91I 194 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AccI 145 GTCTAC GT^[AC][GT]AC 253 GTCTAC AfaI 174 GTAC GT^AC 324 GTAC BsiYI 150 CCGAGCCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 194 CCACGCTCTGG BslI 150 CCGAGCCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 194 CCACGCTCTGG BsrFI 121 ACCGGC [GA]^CCGG[CT] 208 GCCGGC Cfr10I 121 ACCGGC [GA]^CCGG[CT] 208 GCCGGC HgaI 432 GACGC GACGC(5/10) 143 GCGTC MspA1I 181 CCGCTG C[AC]G^C[GT]G 319 CCGCTG PstI 70 CTGCAG CTGCA^G 165 CTGCAG RsaI 174 GTAC GT^AC 324 GTAC SfcI 70 CTGCAG C^T[GA][CT]AG 165 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AccII AciI AluI BbvI BsaJI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MnlI MspI MvaI MvnI MwoI Sau96I ScrFI TseI