################################## # NO CUT ENZYMES ( 190 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccIII 28 TCCGGA T^CCGGA AciI 124 GCGG CCGC(-3/-1) AfaI 84 GTAC GT^AC AlwI 200 GATCC GGATC(4/5) Aor51HI 150 AGCGCT AGC^GCT Asp700I 250 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 105 CCCGGG C^[CT]CG[GA]G BalI 134 TGGCCA TGG^CCA BbsI 274 GAAGAC GAAGAC(2/6) BbvI 153 GCTGC GCAGC(8/12) BfaI 4 CTAG C^TAG BpuAI 274 GAAGAC GAAGAC(2/6) BsaWI 28 TCCGGA [AT]^CCGG[AT] BseAI 28 TCCGGA T^CCGGA BsmAI 211 GAGAC GTCTC(1/5) BsmFI 48 GTCCC GGGAC(10/14) BsoBI 105 CCCGGG C^[CT]CG[GA]G BspEI 28 TCCGGA T^CCGGA BsrDI 283 GCAATG GCAATG(2/0) BsrI 18 ACTGG ACTGG(1/-1) BssSI 55 CTCGTG CACGAG(-5/-1) Cac8I 72 GCTGGC GC[ACGT]^[ACGT]GC DdeI 147 CTCAG C^T[ACGT]AG DpnII 200 GATC ^GATC Eco47III 150 AGCGCT AGC^GCT Fnu4HI 153 GCTGC GC^[ACGT]GC HaeII 150 AGCGCT [GA]GCGC^[CT] HgaI 115 GACGC GACGC(5/10) HphI 176 TCACC GGTGA(8/7) ItaI 153 GCTGC GC^[ACGT]GC MaeI 4 CTAG C^TAG MaeIII 8 GTAAC ^GT[ACGT]AC MboI 200 GATC ^GATC MboII 274 GAAGA GAAGA(8/7) MluNI 134 TGGCCA TGG^CCA MroI 28 TCCGGA T^CCGGA MscI 134 TGGCCA TGG^CCA MspA1I 122 CAGCGG C[AC]G^C[GT]G MwoI 15 GCGACTGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 200 GATC ^GATC NlaIII 132 CATG CATG^ RsaI 84 GTAC GT^AC Sau3AI 200 GATC ^GATC SmaI 105 CCCGGG CCC^GGG TseI 153 GCTGC G^C[AT]GC TspRI 215 CACTG CA[GC]TG[ACGT][ACGT]^ XmaCI 105 CCCGGG C^CCGGG XmaI 105 CCCGGG C^CCGGG XmnI 250 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BcnI 105 CCCGG CC^[GC]GG 106 CCGGG BsiYI 105 CCCGGGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 260 CCAGAACTCGG BslI 105 CCCGGGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 260 CCAGAACTCGG BstNI 63 CCTGG CC^[AT]GG 179 CCAGG CfoI 70 GCGC GCG^C 151 GCGC EaeI 74 TGGCCG [CT]^GGCC[GA] 134 TGGCCA EcoRII 63 CCTGG ^CC[AT]GG 179 CCAGG HaeIII 75 GGCC GG^CC 135 GGCC HhaI 70 GCGC GCG^C 151 GCGC HinP1I 70 GCGC G^CGC 151 GCGC MvaI 63 CCTGG CC^[AT]GG 179 CCAGG NciI 105 CCCGG CC^[GC]GG 106 CCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### BsaJI BssKI HapII HpaII MnlI MspI ScrFI