(Circular) (Six-base) MAPSORT of: pFN21AE1480.seq Check: 5028 from: 1 to: 4996 pFN21AE1480 4996 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2806 278 Size: 53 83 186 936 1270 2468 Fragments arranged by size: 2468 1270 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2339 1937 Size: 402 4594 Acc65I G'GTAC_C Cuts at: 2317 2317 Size: 4996 AclI AA'CG_TT Cuts at: 1563 3123 3496 1563 Size: 1560 373 3063 Fragments arranged by size: 3063 1560 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3059 4443 4711 1632 Size: 1427 1384 268 1917 Fragments arranged by size: 1917 1427 1384 268 AfeI AGC'GCT Cuts at: 2157 2157 Size: 4996 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4799 AflIII A'CryG_T Cuts at: 1203 3895 4872 1203 Size: 2692 977 1327 Fragments arranged by size: 2692 1327 977 AgeI A'CCGG_T Cuts at: 3827 3827 Size: 4996 AhdI GACnn_n'nnGTC Cuts at: 3725 3725 Size: 4996 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4996 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4996 AlwNI CAG_nnn'CTG Cuts at: 2739 4311 2739 Size: 1572 3424 ApaLI G'TGCA_C Cuts at: 3053 4209 3053 Size: 1156 3840 ApoI r'AATT_y Cuts at: 1493 2305 2531 2683 4792 1493 Size: 812 226 152 2109 1697 Fragments arranged by size: 2109 1697 812 226 152 AseI AT'TA_AT Cuts at: 160 1032 3550 160 Size: 872 2518 1606 Fragments arranged by size: 2518 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4996 AvaI C'yCGr_G Cuts at: 1870 1943 2110 2321 1870 Size: 73 167 211 4545 Fragments arranged by size: 4545 211 167 73 BamHI G'GATC_C Cuts at: 2326 2693 2326 Size: 367 4629 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2317 3772 618 Size: 325 217 627 530 1455 1842 Fragments arranged by size: 1842 1455 627 530 325 217 BanII G_rGCy'C Cuts at: 1391 2315 1391 Size: 924 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2286 928 Size: 971 387 3638 Fragments arranged by size: 3638 971 387 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3187 3221 1903 Size: 34 50 34 1166 34 3678 Fragments arranged by size: 3678 1166 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2888 4098 2888 Size: 1210 3786 BclI T'GATC_A Cuts at: 1548 1548 Size: 4996 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3607 136 Size: 107 122 71 631 605 1935 1525 Fragments arranged by size: 1935 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4991 4991 Size: 4996 BlpI GC'TnA_GC Cuts at: 2413 2413 Size: 4996 Bme1580I G_kGCm'C Cuts at: 1790 3057 4213 1790 Size: 1267 1156 2573 Fragments arranged by size: 2573 1267 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4996 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2033 2727 3685 Size: 416 53 83 399 24 616 694 958 Cuts at: 3685 442 Size: 1753 Fragments arranged by size: 1753 958 694 616 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4996 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3656 1239 Size: 2417 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3121 3986 4284 3121 Size: 865 298 3833 Fragments arranged by size: 3833 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3659 882 Size: 617 2160 2219 Fragments arranged by size: 2219 2160 617 BsaAI yAC'GTr Cuts at: 493 1204 2787 493 Size: 711 1583 2702 Fragments arranged by size: 2702 1583 711 BsaBI GATnn'nnATC Cuts at: 4990 4990 Size: 4996 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2803 3185 3820 275 Size: 53 83 186 936 1270 382 635 1451 Fragments arranged by size: 1451 1270 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2033 3429 3827 4101 4248 4659 2033 Size: 1396 398 274 147 411 2370 Fragments arranged by size: 2370 1396 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4771 Fragments arranged by size: 4771 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4996 BseYI C'CCAG_C Cuts at: 4199 4695 4199 Size: 496 4500 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4423 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2446 3207 4235 1540 Size: 275 186 445 761 1028 2301 Fragments arranged by size: 2301 1028 761 445 275 186 BsiHKAI G_wGCw'C Cuts at: 3057 3142 4213 3057 Size: 85 1071 3840 Fragments arranged by size: 3840 1071 85 BsmI GAATG_Cn' Cuts at: 2507 2600 2507 Size: 93 4903 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2315 3057 3142 4213 1391 Size: 399 525 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 525 399 85 BspEI T'CCGG_A Cuts at: 2033 4659 2033 Size: 2626 2370 BspHI T'CATG_A Cuts at: 2885 4615 2885 Size: 1730 3266 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2351 844 Size: 1507 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2883 1394 Size: 429 1060 3507 Fragments arranged by size: 3507 1060 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2295 3491 3665 65 Size: 1183 1047 1196 174 1396 Fragments arranged by size: 1396 1196 1183 1047 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3640 3827 1700 Size: 110 1830 187 2869 Fragments arranged by size: 2869 1830 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4996 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4996 BssSI C'ACGA_G Cuts at: 2060 3056 4068 2060 Size: 996 1012 2988 Fragments arranged by size: 2988 1012 996 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4996 BstBI TT'CG_AA Cuts at: 3800 3800 Size: 4996 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4996 BstYI r'GATC_y Cuts at: 1972 2326 2693 3078 3095 4547 4633 4645 4991 Size: 354 367 385 17 1452 86 12 346 Cuts at: 4991 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 354 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4444 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4257 Fragments arranged by size: 4257 683 56 BtsI GCAGTG_nn' Cuts at: 2512 3306 3326 2512 Size: 794 20 4182 Fragments arranged by size: 4182 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4996 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4961 DraI TTT'AAA Cuts at: 2300 2300 Size: 4996 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4003 809 Size: 507 2687 1802 Fragments arranged by size: 2687 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2139 2443 Size: 54 1425 50 275 18 249 60 304 Cuts at: 2443 3332 4961 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 304 275 249 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2443 1537 Size: 275 631 4090 Fragments arranged by size: 4090 631 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2926 1353 Size: 1573 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3583 3957 4103 387 Size: 1064 138 1994 374 146 1280 Fragments arranged by size: 1994 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3059 3656 4443 4711 1239 Size: 393 1427 597 787 268 1524 Fragments arranged by size: 1524 1427 787 597 393 268 EcoRI G'AATT_C Cuts at: 2305 2683 2305 Size: 378 4618 FspI TGC'GCA Cuts at: 3502 3502 Size: 4996 HaeII r_GCGC'y Cuts at: 1678 2159 4143 1678 Size: 481 1984 2531 Fragments arranged by size: 2531 1984 481 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2028 2060 2154 2186 3637 Size: 32 135 32 456 32 94 32 1451 Cuts at: 3637 3669 3711 3743 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1451 456 135 94 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2340 2586 669 Size: 988 281 402 246 3079 Fragments arranged by size: 3079 988 402 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4996 HpaI GTT'AAC Cuts at: 2586 2586 Size: 4996 KpnI G_GTAC'C Cuts at: 2321 2321 Size: 4996 MfeI C'AATT_G Cuts at: 2595 2595 Size: 4996 MluI A'CGCG_T Cuts at: 4872 4872 Size: 4996 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4110 4294 1305 Size: 2805 184 2007 Fragments arranged by size: 2805 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2141 10 Size: 54 1425 343 309 2865 Fragments arranged by size: 2865 1425 343 309 54 MslI CAynn'nnrTG Cuts at: 518 1166 2954 3313 3472 518 Size: 648 1788 359 159 2042 Fragments arranged by size: 2042 1788 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2129 2412 3089 4237 4482 1606 Size: 523 283 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 523 283 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4444 NdeI CA'TA_TG Cuts at: 387 387 Size: 4996 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4996 NotI GC'GGCC_GC Cuts at: 2443 2443 Size: 4996 NspI r_CATG'y Cuts at: 1132 1229 2354 3899 1132 Size: 97 1125 1545 2229 Fragments arranged by size: 2229 1545 1125 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4996 PciI A'CATG_T Cuts at: 3895 3895 Size: 4996 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4996 PmeI GTTT'AAAC Cuts at: 2300 2300 Size: 4996 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3065 3097 4578 4610 3065 Size: 32 1481 32 3451 Fragments arranged by size: 3451 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4996 PsiI TTA'TAA Cuts at: 2566 2566 Size: 4996 PstI C_TGCA'G Cuts at: 830 2348 830 Size: 1518 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4996 SalI G'TCGA_C Cuts at: 1936 2338 1936 Size: 402 4594 SbfI CC_TGCA'GG Cuts at: 2348 2348 Size: 4996 ScaI AGT'ACT Cuts at: 3244 4956 3244 Size: 1712 3284 SfcI C'TryA_G Cuts at: 826 1045 2344 3479 4160 4351 826 Size: 219 1299 1135 681 191 1471 Fragments arranged by size: 1471 1299 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4996 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4996 SmaI CCC'GGG Cuts at: 1872 2112 2323 1872 Size: 240 211 4545 Fragments arranged by size: 4545 240 211 SmlI C'TyrA_G Cuts at: 820 1017 1943 3100 4001 4263 820 Size: 197 926 1157 901 262 1553 Fragments arranged by size: 1553 1157 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2787 493 Size: 2294 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4996 SphI G_CATG'C Cuts at: 1132 2354 1132 Size: 1222 3774 SspI AAT'ATT Cuts at: 5 52 2920 5 Size: 47 2868 2081 Fragments arranged by size: 2868 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4996 StyI C'CwwG_G Cuts at: 513 1065 2435 513 Size: 552 1370 3074 Fragments arranged by size: 3074 1370 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 3023 3040 3193 3378 3823 325 Size: 1255 480 963 17 153 185 445 1498 Fragments arranged by size: 1498 1255 963 480 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3242 4954 96 Size: 274 80 33 51 1443 1265 1712 138 Fragments arranged by size: 1712 1443 1265 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4956 4988 4956 Size: 32 4964 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4996 XbaI T'CTAG_A Cuts at: 2332 2332 Size: 4996 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4996 XmaI C'CCGG_G Cuts at: 1870 2110 2321 1870 Size: 240 211 4545 Fragments arranged by size: 4545 240 211 XmnI GAAnn'nnTTC Cuts at: 2259 3125 2259 Size: 866 4130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2804 276 Size: 53 83 186 936 1270 2468 Fragments arranged by size: 2468 1270 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI