################################## # NO CUT ENZYMES ( 217 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaI HpaII HphI ItaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI TfiI Tru9I TseI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AspHI 196 GAGCTC G[AT]GC[AT]^C AvaII 21 GGACC G^G[AT]CC BamHI 150 GGATCC G^GATCC BanII 196 GAGCTC G[GA]GC[CT]^C BfaI 94 CTAG C^TAG BmyI 196 GAGCTC G[AGT]GC[ACT]^C BsiHKAI 196 GAGCTC G[AT]GC[AT]^C BsmI 89 GAATGC GAATGC(1/-1) Bsp1286I 196 GAGCTC G[AGT]GC[ACT]^C BstF5I 168 CATCC GGATG(2/0) BstYI 150 GGATCC [GA]^GATC[CT] Cfr13I 21 GGACC G^G[ACGT]CC DraII 20 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO109I 20 AGGACCT [GA]G^G[ACGT]CC[CT] FokI 168 CATCC GGATG(9/13) HincII 55 GTTGAC GT[CT]^[GA]AC HindII 55 GTTGAC GT[CT]^[GA]AC MaeI 94 CTAG C^TAG MaeII 128 ACGT A^CGT MaeIII 76 GTGAC ^GT[ACGT]AC MflI 150 GGATCC [GA]^GATC[CT] NlaIV 150 GGATCC GG[ACGT]^[ACGT]CC PpuMI 20 AGGACCT [GA]G^G[AT]CC[CT] SacI 196 GAGCTC GAGCT^C Sau96I 21 GGACC G^G[ACGT]CC SfaNI 167 GCATC GCATC(5/9) SmlI 64 CTCAAG C^T[CT][GA]AG SspI 13 AATATT AAT^ATT Tsp45I 76 GTGAC ^GT[GC]AC Tsp509I 41 AATT ^AATT XhoII 150 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AluI 62 AGCT AG^CT 197 AGCT AlwI 151 GATCC GGATC(4/5) 150 GGATC DpnII 140 GATC ^GATC 151 GATC MboI 140 GATC ^GATC 151 GATC NdeII 140 GATC ^GATC 151 GATC Sau3AI 140 GATC ^GATC 151 GATC TspRI 80 CACTG CA[GC]TG[ACGT][ACGT]^ 155 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### BsrI MboII MnlI