################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII KasI KpnI Ksp632I KspI MamI MfeI MluI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccIII 158 TCCGGA T^CCGGA AlwNI 1031 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 559 CCTGAGG CC^T[ACGT]AGG AspHI 861 GAGCTC G[AT]GC[AT]^C AvaI 115 CTCGGG C^[CT]CG[GA]G BamHI 77 GGATCC G^GATCC BbsI 337 GTCTTC GAAGAC(2/6) BfaI 460 CTAG C^TAG BpmI 961 CTGGAG CTGGAG(16/14) BpuAI 337 GTCTTC GAAGAC(2/6) BseAI 158 TCCGGA T^CCGGA BsiHKAI 861 GAGCTC G[AT]GC[AT]^C BsoBI 115 CTCGGG C^[CT]CG[GA]G BspEI 158 TCCGGA T^CCGGA BstEII 102 GGTCACC G^GT[ACGT]ACC BstPI 102 GGTCACC G^GT[ACGT]ACC Bsu36I 559 CCTGAGG CC^T[ACGT]AGG DraIII 304 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 559 CCTGAGG CC^T[ACGT]AGG EcoNI 972 CCTGTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 102 GGTCACC G^GT[ACGT]ACC HincII 850 GTTAAC GT[CT]^[GA]AC HindII 850 GTTAAC GT[CT]^[GA]AC HpaI 850 GTTAAC GTT^AAC MaeI 460 CTAG C^TAG MaeII 905 ACGT A^CGT MroI 158 TCCGGA T^CCGGA PstI 1028 CTGCAG CTGCA^G PvuII 882 CAGCTG CAG^CTG SacI 861 GAGCTC GAGCT^C ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 527 GTAC GT^AC 828 GTAC ApaI 395 GGGCCC GGGCC^C 968 GGGCCC BanI 553 GGCACC G^G[CT][GA]CC 790 GGCACC BsaWI 158 TCCGGA [AT]^CCGG[AT] 611 TCCGGT BseRI 20 CTCCTC GAGGAG(10/8) 562 GAGGAG BsmFI 515 GGGAC GGGAC(10/14) 713 GTCCC BstXI 251 CCATGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 953 CCACCAGTCTGG BstYI 77 GGATCC [GA]^GATC[CT] 680 AGATCC Cac8I 58 GCTGGC GC[ACGT]^[ACGT]GC 632 GCTTGC DsaI 251 CCATGG C^C[GA][CT]GG 257 CCATGG HapII 159 CCGG C^CGG 612 CCGG HpaII 159 CCGG C^CGG 612 CCGG MflI 77 GGATCC [GA]^GATC[CT] 680 AGATCC MspA1I 366 CCGCTG C[AC]G^C[GT]G 882 CAGCTG MspI 159 CCGG C^CGG 612 CCGG MwoI 733 GCTGTGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1009 GCCAATTCTGC NcoI 251 CCATGG C^CATGG 257 CCATGG PpuMI 119 GGGTCCT [GA]G^G[AT]CC[CT] 722 GGGTCCT RsaI 527 GTAC GT^AC 828 GTAC SfaNI 384 GCATC GCATC(5/9) 541 GCATC SfcI 494 CTACAG C^T[GA][CT]AG 1028 CTGCAG TfiI 155 GATTC G^A[AT]TC 450 GATTC Tsp45I 103 GTCAC ^GT[GC]AC 175 GTCAC Tsp509I 300 AATT ^AATT 1012 AATT XhoII 77 GGATCC [GA]^GATC[CT] 680 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AluI AlwI AvaII BalI BanII BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MvaI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI StyI Tru9I TseI TspRI