################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanII BbsI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MfeI MluI MroI MslI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 244 GACGTC GACGT^C AccI 661 GTAGAC GT^[AC][GT]AC AlwNI 1136 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1369 CCTGAGG CC^T[ACGT]AGG Asp700I 7 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 252 GAGCAC G[AT]GC[AT]^C BalI 91 TGGCCA TGG^CCA BbvCI 306 CCTCAGC CCTCAGC(-5/-2) BglI 386 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 26 CTGGAG CTGGAG(16/14) BsaBI 554 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 1053 CTGCAC GTGCAG(16/14) BsiHKAI 252 GAGCAC G[AT]GC[AT]^C BsmFI 400 GGGAC GGGAC(10/14) BsmI 1470 GAATGC GAATGC(1/-1) Bsp1407I 992 TGTACA T^GTACA BspLU11I 995 ACATGT A^CATGT BspMI 546 GCAGGT ACCTGC(4/8) BsrGI 992 TGTACA T^GTACA BstAPI 212 GCACAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 553 GGATCT [GA]^GATC[CT] Bsu36I 1369 CCTGAGG CC^T[ACGT]AGG DsaI 1112 CCGTGG C^C[GA][CT]GG Eco81I 1369 CCTGAGG CC^T[ACGT]AGG EcoRI 1525 GAATTC G^AATTC HgaI 975 GACGC GACGC(5/10) MamI 554 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 553 GGATCT [GA]^GATC[CT] MluNI 91 TGGCCA TGG^CCA MscI 91 TGGCCA TGG^CCA MspA1I 388 CAGCTG C[AC]G^C[GT]G PflMI 486 CCATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1178 CTGCAG CTGCA^G PvuII 388 CAGCTG CAG^CTG SmlI 1234 CTCAAG C^T[CT][GA]AG SspBI 992 TGTACA T^GTACA Van91I 486 CCATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 387 CCAGCTGGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 553 GGATCT [GA]^GATC[CT] XmnI 7 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AvaII 322 GGTCC G^G[AT]CC 1593 GGACC BanI 844 GGCGCC G^G[CT][GA]CC 1100 GGCGCC BbeI 844 GGCGCC GGCGC^C 1100 GGCGCC BbrPI 988 CACGTG CAC^GTG 1095 CACGTG BcgI 1062 CGACGTGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1464 CGAGGAGAATGC BfaI 1195 CTAG C^TAG 1390 CTAG BsaAI 988 CACGTG [CT]AC^GT[GA] 1095 CACGTG BsmAI 29 GAGAC GTCTC(1/5) 270 GAGAC BsrDI 164 GCAATG GCAATG(2/0) 395 GCAATG BstF5I 768 CATCC GGATG(2/0) 1281 GGATG CfoI 845 GCGC GCG^C 1101 GCGC DraI 282 TTTAAA TTT^AAA 1645 TTTAAA EaeI 91 TGGCCA [CT]^GGCC[GA] 968 CGGCCA EarI 587 GAAGAG CTCTTC(1/4) 1152 GAAGAG FauI 1032 CCCGC CCCGC(4/6) 1087 CCCGC FokI 768 CATCC GGATG(9/13) 1281 GGATG HaeII 844 GGCGCC [GA]GCGC^[CT] 1100 GGCGCC HhaI 845 GCGC GCG^C 1101 GCGC HinP1I 845 GCGC G^CGC 1101 GCGC KasI 844 GGCGCC G^GCGCC 1100 GGCGCC Ksp632I 587 GAAGAG CTCTTC(1/4) 1152 GAAGAG MaeI 1195 CTAG C^TAG 1390 CTAG NarI 844 GGCGCC GG^CGCC 1100 GGCGCC NspI 631 GCATGT [GA]CATG^[CT] 995 ACATGT PmaCI 988 CACGTG CAC^GTG 1095 CACGTG PmlI 988 CACGTG CAC^GTG 1095 CACGTG SfoI 844 GGCGCC GGC^GCC 1100 GGCGCC TfiI 277 GATTC G^A[AT]TC 1239 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI ApoI BbvI BcnI BmyI BsaHI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HapII Hin1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I