(Circular) (Six-base) MAPSORT of: pFN21AE3908.seq Check: 4585 from: 1 to: 6358 pFN21AE3908 6358 bp With 182 enzymes: SgfI * March 28, 2012 11:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2253 4168 278 Size: 53 83 186 936 717 1915 2468 Fragments arranged by size: 2468 1915 936 717 186 83 53 AccI GT'mk_AC Cuts at: 1937 2667 3701 1937 Size: 730 1034 4594 Fragments arranged by size: 4594 1034 730 Acc65I G'GTAC_C Cuts at: 3679 3679 Size: 6358 AclI AA'CG_TT Cuts at: 1563 4485 4858 1563 Size: 2922 373 3063 Fragments arranged by size: 3063 2922 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2139 3176 3212 3523 3632 4421 5805 6073 Size: 507 1037 36 311 109 789 1384 268 Cuts at: 6073 1632 Size: 1917 Fragments arranged by size: 1917 1384 1037 789 507 311 268 109 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6161 AflIII A'CryG_T Cuts at: 1203 2964 2994 3000 3069 5257 6234 1203 Size: 1761 30 6 69 2188 977 1327 Fragments arranged by size: 2188 1761 1327 977 69 30 6 AgeI A'CCGG_T Cuts at: 5189 5189 Size: 6358 AhdI GACnn_n'nnGTC Cuts at: 5087 5087 Size: 6358 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6358 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6358 AlwNI CAG_nnn'CTG Cuts at: 3146 4101 5673 3146 Size: 955 1572 3831 Fragments arranged by size: 3831 1572 955 ApaLI G'TGCA_C Cuts at: 4415 5571 4415 Size: 1156 5202 ApoI r'AATT_y Cuts at: 1493 3164 3308 3530 3667 3893 4045 6154 1493 Size: 1671 144 222 137 226 152 2109 1697 Fragments arranged by size: 2109 1697 1671 226 222 152 144 137 AseI AT'TA_AT Cuts at: 160 1032 4912 160 Size: 872 3880 1606 Fragments arranged by size: 3880 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 AvaI C'yCGr_G Cuts at: 1870 1943 3683 1870 Size: 73 1740 4545 Fragments arranged by size: 4545 1740 73 BamHI G'GATC_C Cuts at: 3688 4055 3688 Size: 367 5991 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2849 3105 3679 5134 618 Size: 325 217 627 1062 256 574 1455 1842 Fragments arranged by size: 1842 1455 1062 627 574 325 256 217 BanII G_rGCy'C Cuts at: 1391 3677 1391 Size: 2286 4072 BbeI G_GCGC'C Cuts at: 2853 3109 2853 Size: 256 6102 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5387 BbvCI CC'TCA_GC Cuts at: 2312 2312 Size: 6358 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3056 3090 3458 3492 4549 4583 1903 Size: 34 1119 34 368 34 1057 34 3678 Fragments arranged by size: 3678 1119 1057 368 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4250 5460 4250 Size: 1210 5148 BclI T'GATC_A Cuts at: 1548 1548 Size: 6358 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2397 4969 136 Size: 107 122 71 631 605 725 2572 1525 Fragments arranged by size: 2572 1525 725 631 605 122 107 71 BglII A'GATC_T Cuts at: 6353 6353 Size: 6358 BlpI GC'TnA_GC Cuts at: 3775 3775 Size: 6358 Bme1580I G_kGCm'C Cuts at: 1790 2430 2876 2994 4419 5575 1790 Size: 640 446 118 1425 1156 2573 Fragments arranged by size: 2573 1425 1156 640 446 118 BmgBI CAC'GTC Cuts at: 1318 2967 3070 1318 Size: 1649 103 4606 Fragments arranged by size: 4606 1649 103 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4089 5047 442 Size: 416 53 83 399 24 2672 958 1753 Fragments arranged by size: 2672 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6358 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2052 5018 1239 Size: 813 2966 2579 Fragments arranged by size: 2966 2579 813 Bpu10I CC'TnA_GC Cuts at: 2312 2312 Size: 6358 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3224 4483 5348 5646 3224 Size: 1259 865 298 3936 Fragments arranged by size: 3936 1259 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5021 882 Size: 617 3522 2219 Fragments arranged by size: 3522 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2995 3102 4149 493 Size: 711 1791 107 1047 2702 Fragments arranged by size: 2702 1791 1047 711 107 BsaBI GATnn'nnATC Cuts at: 2563 6352 2563 Size: 3789 2569 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2250 2850 3106 4165 Size: 53 83 186 936 717 600 256 1059 Cuts at: 4165 4547 5182 275 Size: 382 635 1451 Fragments arranged by size: 1451 1059 936 717 635 600 382 256 186 83 53 BsaWI w'CCGG_w Cuts at: 4791 5189 5463 5610 6021 4791 Size: 398 274 147 411 5128 Fragments arranged by size: 5128 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6133 Fragments arranged by size: 6133 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3101 3392 3485 1186 Size: 1915 291 93 4059 Fragments arranged by size: 4059 1915 291 93 BseYI C'CCAG_C Cuts at: 2395 5561 6057 2395 Size: 3166 496 2696 Fragments arranged by size: 3166 2696 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3043 1163 Size: 573 1307 4478 Fragments arranged by size: 4478 1307 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3808 4569 5597 1540 Size: 275 186 1807 761 1028 2301 Fragments arranged by size: 2301 1807 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2261 4419 4504 5575 2261 Size: 2158 85 1071 3044 Fragments arranged by size: 3044 2158 1071 85 BsmI GAATG_Cn' Cuts at: 3481 3869 3962 3481 Size: 388 93 5877 Fragments arranged by size: 5877 388 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2261 2430 2876 2994 3677 4419 4504 Size: 399 471 169 446 118 683 742 85 Cuts at: 4504 5575 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 683 471 446 399 169 118 85 BspEI T'CCGG_A Cuts at: 6021 6021 Size: 6358 BspHI T'CATG_A Cuts at: 4247 5977 4247 Size: 1730 4628 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2542 3713 844 Size: 1698 1171 3489 Fragments arranged by size: 3489 1698 1171 BsrBI CCG'CTC Cuts at: 1394 1823 4245 1394 Size: 429 2422 3507 Fragments arranged by size: 3507 2422 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2176 2407 4853 5027 65 Size: 1183 928 231 2446 174 1396 Fragments arranged by size: 2446 1396 1183 928 231 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5002 5189 1700 Size: 110 3192 187 2869 Fragments arranged by size: 3192 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2997 96 Size: 2901 3457 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6358 BssSI C'ACGA_G Cuts at: 4418 5430 4418 Size: 1012 5346 BstAPI GCAn_nnn'nTGC Cuts at: 794 2223 794 Size: 1429 4929 BstBI TT'CG_AA Cuts at: 5162 5162 Size: 6358 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6358 BstYI r'GATC_y Cuts at: 1972 2558 3688 4055 4440 4457 5909 5995 6007 Size: 586 1130 367 385 17 1452 86 12 Cuts at: 6007 6353 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1130 586 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3375 3375 Size: 6358 BtgI C'CryG_G Cuts at: 513 1065 2004 3117 513 Size: 552 939 1113 3754 Fragments arranged by size: 3754 1113 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3466 487 Size: 683 56 2240 3379 Fragments arranged by size: 3379 2240 683 56 BtsI GCAGTG_nn' Cuts at: 2167 3180 3874 4668 4688 2167 Size: 1013 694 794 20 3837 Fragments arranged by size: 3837 1013 794 694 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6358 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6323 DraI TTT'AAA Cuts at: 2289 3652 3662 2289 Size: 1363 10 4985 Fragments arranged by size: 4985 1363 10 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5365 809 Size: 507 4049 1802 Fragments arranged by size: 4049 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2096 2973 3805 Size: 54 1425 50 275 18 266 877 832 Cuts at: 3805 4694 6323 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 877 832 275 266 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3805 1537 Size: 275 1993 4090 Fragments arranged by size: 4090 1993 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2587 3152 4288 1353 Size: 1234 565 1136 3423 Fragments arranged by size: 3423 1234 1136 565 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4945 5319 5465 387 Size: 1064 138 3356 374 146 1280 Fragments arranged by size: 3356 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2052 2139 3176 3212 3523 3632 4421 Size: 393 420 87 1037 36 311 109 789 Cuts at: 4421 5018 5805 6073 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1037 789 787 597 420 393 311 268 109 87 36 EcoRI G'AATT_C Cuts at: 3530 3667 4045 3530 Size: 137 378 5843 Fragments arranged by size: 5843 378 137 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2033 2033 Size: 6358 FspI TGC'GCA Cuts at: 4864 4864 Size: 6358 HaeII r_GCGC'y Cuts at: 1678 2853 3109 5505 1678 Size: 1175 256 2396 2531 Fragments arranged by size: 2531 2396 1175 256 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2398 2430 4999 5031 5073 Size: 32 135 32 826 32 2569 32 42 Cuts at: 5073 5105 1373 Size: 32 2626 Fragments arranged by size: 2626 2569 826 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3702 3948 669 Size: 988 281 1764 246 3079 Fragments arranged by size: 3079 1764 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6358 HpaI GTT'AAC Cuts at: 3948 3948 Size: 6358 KasI G'GCGC_C Cuts at: 2849 3105 2849 Size: 256 6102 KpnI G_GTAC'C Cuts at: 3683 3683 Size: 6358 MfeI C'AATT_G Cuts at: 3957 3957 Size: 6358 MluI A'CGCG_T Cuts at: 6234 6234 Size: 6358 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3622 5472 5656 1305 Size: 2317 1850 184 2007 Fragments arranged by size: 2317 2007 1850 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2098 10 Size: 54 1425 343 266 4270 Fragments arranged by size: 4270 1425 343 266 54 MslI CAynn'nnrTG Cuts at: 518 1166 4316 4675 4834 518 Size: 648 3150 359 159 2042 Fragments arranged by size: 3150 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2395 3774 4451 5599 5844 1606 Size: 789 1379 677 1148 245 2120 Fragments arranged by size: 2120 1379 1148 789 677 245 NarI GG'CG_CC Cuts at: 2850 3106 2850 Size: 256 6102 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4867 Fragments arranged by size: 4867 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6358 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6358 NotI GC'GGCC_GC Cuts at: 3805 3805 Size: 6358 NspI r_CATG'y Cuts at: 1132 1229 2640 3004 3716 5261 1132 Size: 97 1411 364 712 1545 2229 Fragments arranged by size: 2229 1545 1411 712 364 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6358 PciI A'CATG_T Cuts at: 3000 5257 3000 Size: 2257 4101 PflMI CCAn_nnn'nTGG Cuts at: 1108 2497 1108 Size: 1389 4969 PfoI T'CCnGG_A Cuts at: 3336 3336 Size: 6358 PmeI GTTT'AAAC Cuts at: 3662 3662 Size: 6358 PmlI CAC'GTG Cuts at: 2995 3102 2995 Size: 107 6251 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4427 4459 5940 5972 4427 Size: 32 1481 32 4813 Fragments arranged by size: 4813 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6358 PsiI TTA'TAA Cuts at: 2538 3928 2538 Size: 1390 4968 PstI C_TGCA'G Cuts at: 830 3187 3710 830 Size: 2357 523 3478 Fragments arranged by size: 3478 2357 523 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6358 PvuII CAG'CTG Cuts at: 2395 2395 Size: 6358 SalI G'TCGA_C Cuts at: 1936 3700 1936 Size: 1764 4594 SbfI CC_TGCA'GG Cuts at: 3710 3710 Size: 6358 ScaI AGT'ACT Cuts at: 4606 6318 4606 Size: 1712 4646 SfcI C'TryA_G Cuts at: 826 1045 2129 2187 3183 3564 3706 4841 5522 Size: 219 1084 58 996 381 142 1135 681 Cuts at: 5522 5713 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1084 996 681 381 219 191 142 58 SfoI GGC'GCC Cuts at: 2851 3107 2851 Size: 256 6102 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6358 SmaI CCC'GGG Cuts at: 1872 3685 1872 Size: 1813 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3239 4462 5363 5625 820 Size: 197 926 1296 1223 901 262 1553 Fragments arranged by size: 1553 1296 1223 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4149 493 Size: 3656 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6358 SphI G_CATG'C Cuts at: 1132 3716 1132 Size: 2584 3774 SspI AAT'ATT Cuts at: 5 52 4282 5 Size: 47 4230 2081 Fragments arranged by size: 4230 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6358 StyI C'CwwG_G Cuts at: 513 1065 2004 3797 513 Size: 552 939 1793 3074 Fragments arranged by size: 3074 1793 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3062 4385 4402 4555 4740 5185 325 Size: 1255 1482 1323 17 153 185 445 1498 Fragments arranged by size: 1498 1482 1323 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2050 2997 3213 Size: 274 80 33 51 1443 73 947 216 Cuts at: 3213 4604 6316 96 Size: 1391 1712 138 Fragments arranged by size: 1712 1443 1391 947 274 216 138 80 73 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2322 2354 3216 3248 6318 6350 2322 Size: 32 862 32 3070 32 2330 Fragments arranged by size: 3070 2330 862 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6358 XbaI T'CTAG_A Cuts at: 3694 3694 Size: 6358 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2399 2399 Size: 6358 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6358 XmaI C'CCGG_G Cuts at: 1870 3683 1870 Size: 1813 4545 XmnI GAAnn'nnTTC Cuts at: 2016 4487 2016 Size: 2471 3887 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2251 4166 276 Size: 53 83 186 936 717 1915 2468 Fragments arranged by size: 2468 1915 936 717 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI