Restriction map for
pFN21AB5673
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
CelII ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 579 GTCTAC GT^[AC][GT]AC
AciI 646 CCGC CCGC(-3/-1)
AocI 58 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1072 AGCGCT AGC^GCT
AvaI 827 CTCGGG C^[CT]CG[GA]G
BanII 1063 GAGCCC G[GA]GC[CT]^C
BbsI 70 GAAGAC GAAGAC(2/6)
BbvCI 459 CCTCAGC CCTCAGC(-5/-2)
BbvI 1339 GCTGC GCAGC(8/12)
BcnI 755 CCCGG CC^[GC]GG
BpmI 344 CTCCAG CTGGAG(16/14)
BpuAI 70 GAAGAC GAAGAC(2/6)
BsaAI 1642 TACGTG [CT]AC^GT[GA]
BsaBI 1328 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 442 CTCCTC GAGGAG(10/8)
BsmFI 96 GTCCC GGGAC(10/14)
BsoBI 827 CTCGGG C^[CT]CG[GA]G
BspHI 680 TCATGA T^CATGA
BspLU11I 428 ACATGT A^CATGT
BsrDI 1529 GCAATG GCAATG(2/0)
BsrFI 32 ACCGGC [GA]^CCGG[CT]
BssSI 1076 CTCGTG CACGAG(-5/-1)
Bsu36I 58 CCTGAGG CC^T[ACGT]AGG
Cac8I 1160 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 32 ACCGGC [GA]^CCGG[CT]
DsaI 666 CCATGG C^C[GA][CT]GG
EaeI 1618 CGGCCA [CT]^GGCC[GA]
Eco47III 1072 AGCGCT AGC^GCT
Eco81I 58 CCTGAGG CC^T[ACGT]AGG
Fnu4HI 1339 GCTGC GC^[ACGT]GC
ItaI 1339 GCTGC GC^[ACGT]GC
MamI 1328 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1100 TTAA T^TAA
MspA1I 646 CCGCTG C[AC]G^C[GT]G
NciI 755 CCCGG CC^[GC]GG
NcoI 666 CCATGG C^CATGG
NdeI 719 CATATG CA^TATG
NlaIV 1062 GGAGCC GG[ACGT]^[ACGT]CC
NspI 428 ACATGT [GA]CATG^[CT]
RcaI 680 TCATGA T^CATGA
SexAI 559 ACCTGGT A^CC[AT]GGT
SfaNI 253 GCATC GCATC(5/9)
Tru9I 1100 TTAA T^TAA
TseI 1339 GCTGC G^C[AT]GC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 538 AAATTT [GA]^AATT[CT]
837 AAATTC
AflIII 428 ACATGT A^C[GA][CT]GT
1643 ACGTGT
AlwI 651 GATCC GGATC(4/5)
566 GGATC
ApoI 538 AAATTT [GA]^AATT[CT]
837 AAATTC
BsmAI 103 GAGAC GTCTC(1/5)
21 GTCTC
BstF5I 643 CATCC GGATG(2/0)
1202 GGATG
BstXI 1598 CCACATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1621 CCACGTTCCTGG
EcoT14I 666 CCATGG C^C[AT][AT]GG
1388 CCTTGG
FokI 643 CATCC GGATG(9/13)
1202 GGATG
HaeII 374 GGCGCT [GA]GCGC^[CT]
1072 AGCGCT
HincII 159 GTTGAC GT[CT]^[GA]AC
880 GTCAAC
HindII 159 GTTGAC GT[CT]^[GA]AC
880 GTCAAC
HphI 7 GGTGA GGTGA(8/7)
1195 GGTGA
MslI 214 CACTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1378 CACGAATATG
PleI 94 GAGTC GAGTC(4/5)
1535 GAGTC
SapI 1422 GCTCTTC GCTCTTC(1/4)
1431 GCTCTTC
StyI 666 CCATGG C^C[AT][AT]GG
1388 CCTTGG
TaqI 120 TCGA T^CGA
773 TCGA
TthHB8I 120 TCGA T^CGA
773 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AfaI AluI AspHI AvaII BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstNI CfoI
Cfr13I DdeI DpnII EarI EcoRII
HaeIII HapII HhaI HinP1I HinfI
HpaII Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIII RsaI Sau3AI
Sau96I ScrFI SfcI SmlI TfiI
Tsp45I Tsp509I TspRI