(Circular) (Six-base) MAPSORT of: pFN21AB5673.seq Check: 3608 from: 1 to: 6355 pFN21AB5673 6355 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4165 278 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2585 3698 1937 Size: 648 1113 4594 Fragments arranged by size: 4594 1113 648 Acc65I G'GTAC_C Cuts at: 3676 3676 Size: 6355 AclI AA'CG_TT Cuts at: 1563 4482 4855 1563 Size: 2919 373 3063 Fragments arranged by size: 3063 2919 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3058 4418 5802 6070 1632 Size: 1426 1360 1384 268 1917 Fragments arranged by size: 1917 1426 1384 1360 268 AfeI AGC'GCT Cuts at: 3079 3079 Size: 6355 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6158 AflIII A'CryG_T Cuts at: 1203 2433 3648 5254 6231 1203 Size: 1230 1215 1606 977 1327 Fragments arranged by size: 1606 1327 1230 1215 977 AgeI A'CCGG_T Cuts at: 5186 5186 Size: 6355 AhdI GACnn_n'nnGTC Cuts at: 5084 5084 Size: 6355 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6355 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6355 AlwNI CAG_nnn'CTG Cuts at: 4098 5670 4098 Size: 1572 4783 ApaLI G'TGCA_C Cuts at: 4412 5568 4412 Size: 1156 5199 ApoI r'AATT_y Cuts at: 1493 2543 2842 3664 3890 4042 6151 1493 Size: 1050 299 822 226 152 2109 1697 Fragments arranged by size: 2109 1697 1050 822 299 226 152 AseI AT'TA_AT Cuts at: 160 1032 4909 160 Size: 872 3877 1606 Fragments arranged by size: 3877 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 AvaI C'yCGr_G Cuts at: 1870 1943 2832 3680 1870 Size: 73 889 848 4545 Fragments arranged by size: 4545 889 848 73 BamHI G'GATC_C Cuts at: 3685 4052 3685 Size: 367 5988 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3676 5131 618 Size: 325 217 627 1889 1455 1842 Fragments arranged by size: 1889 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3072 3674 1391 Size: 1681 602 4072 Fragments arranged by size: 4072 1681 602 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2082 928 Size: 971 183 5201 Fragments arranged by size: 5201 971 183 BbvCI CC'TCA_GC Cuts at: 2465 2465 Size: 6355 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4546 4580 1903 Size: 34 2609 34 3678 Fragments arranged by size: 3678 2609 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4247 5457 4247 Size: 1210 5145 BclI T'GATC_A Cuts at: 1548 1548 Size: 6355 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4966 136 Size: 107 122 71 631 605 3294 1525 Fragments arranged by size: 3294 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6350 6350 Size: 6355 BlpI GC'TnA_GC Cuts at: 3772 3772 Size: 6355 Bme1580I G_kGCm'C Cuts at: 1790 3043 4416 5572 1790 Size: 1253 1373 1156 2573 Fragments arranged by size: 2573 1373 1253 1156 BmgBI CAC'GTC Cuts at: 1318 3237 3578 1318 Size: 1919 341 4095 Fragments arranged by size: 4095 1919 341 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4086 5044 442 Size: 416 53 83 399 24 2669 958 1753 Fragments arranged by size: 2669 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6355 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2364 3563 2364 Size: 1199 5156 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2334 5015 1239 Size: 1095 2681 2579 Fragments arranged by size: 2681 2579 1095 Bpu10I CC'TnA_GC Cuts at: 2465 2850 2465 Size: 385 5970 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2531 3427 3546 4480 5345 5643 2531 Size: 896 119 934 865 298 3243 Fragments arranged by size: 3243 934 896 865 298 119 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5018 882 Size: 617 3519 2219 Fragments arranged by size: 3519 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3649 4146 493 Size: 711 2445 497 2702 Fragments arranged by size: 2702 2445 711 497 BsaBI GATnn'nnATC Cuts at: 3337 6349 3337 Size: 3012 3343 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4162 4544 5179 275 Size: 53 83 186 936 2629 382 635 1451 Fragments arranged by size: 2629 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4788 5186 5460 5607 6018 4788 Size: 398 274 147 411 5125 Fragments arranged by size: 5125 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6130 Fragments arranged by size: 6130 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2438 1186 Size: 1252 5103 BseYI C'CCAG_C Cuts at: 5558 6054 5558 Size: 496 5859 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5782 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3805 4566 5594 1540 Size: 275 186 1804 761 1028 2301 Fragments arranged by size: 2301 1804 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2347 3429 3438 4416 4501 5572 2347 Size: 1082 9 978 85 1071 3130 Fragments arranged by size: 3130 1082 1071 978 85 9 BsmI GAATG_Cn' Cuts at: 3866 3959 3866 Size: 93 6262 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2347 3043 3072 3429 3438 3674 4416 Size: 399 557 696 29 357 9 236 742 Cuts at: 4416 4501 5572 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 696 557 399 357 236 85 29 9 BspEI T'CCGG_A Cuts at: 6018 6018 Size: 6355 BspHI T'CATG_A Cuts at: 2685 4244 5974 2685 Size: 1559 1730 3066 Fragments arranged by size: 3066 1730 1559 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3710 844 Size: 2866 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4242 1394 Size: 429 2419 3507 Fragments arranged by size: 3507 2419 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3541 4850 5024 65 Size: 1183 2293 1309 174 1396 Fragments arranged by size: 2293 1396 1309 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2037 4999 5186 1700 Size: 110 227 2962 187 2869 Fragments arranged by size: 2962 2869 227 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6355 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6355 BssSI C'ACGA_G Cuts at: 3081 4415 5427 3081 Size: 1334 1012 4009 Fragments arranged by size: 4009 1334 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6355 BstBI TT'CG_AA Cuts at: 5159 5159 Size: 6355 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6355 BstXI CCAn_nnnn'nTGG Cuts at: 3610 3633 3610 Size: 23 6332 BstYI r'GATC_y Cuts at: 1972 3685 4052 4437 4454 5906 5992 6004 6350 Size: 1713 367 385 17 1452 86 12 346 Cuts at: 6350 1972 Size: 1977 Fragments arranged by size: 1977 1713 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2064 2064 Size: 6355 BtgI C'CryG_G Cuts at: 513 1065 2004 2671 513 Size: 552 939 667 4197 Fragments arranged by size: 4197 939 667 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5616 Fragments arranged by size: 5616 683 56 BtsI GCAGTG_nn' Cuts at: 2744 3160 3871 4665 4685 2744 Size: 416 711 794 20 4414 Fragments arranged by size: 4414 794 711 416 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6355 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6320 DraI TTT'AAA Cuts at: 3659 3659 Size: 6355 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5362 809 Size: 507 4046 1802 Fragments arranged by size: 4046 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3623 3802 4691 Size: 54 1425 50 275 18 1793 179 889 Cuts at: 4691 6320 8 Size: 1629 43 Fragments arranged by size: 1793 1629 1425 889 275 179 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3802 1537 Size: 275 1990 4090 Fragments arranged by size: 4090 1990 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2010 2672 3140 3434 3443 4285 1353 Size: 657 662 468 294 9 842 3423 Fragments arranged by size: 3423 842 662 657 468 294 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4942 5316 5462 387 Size: 1064 138 3353 374 146 1280 Fragments arranged by size: 3353 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2334 3058 4418 5015 5802 6070 1239 Size: 393 702 724 1360 597 787 268 1524 Fragments arranged by size: 1524 1360 787 724 702 597 393 268 EcoRI G'AATT_C Cuts at: 3664 4042 3664 Size: 378 5977 FspI TGC'GCA Cuts at: 4861 4861 Size: 6355 HaeII r_GCGC'y Cuts at: 1678 2383 3081 5502 1678 Size: 705 698 2421 2531 Fragments arranged by size: 2531 2421 705 698 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 2632 2647 2664 Size: 32 135 32 437 32 591 15 17 Cuts at: 2664 2679 4996 5028 5070 5102 1373 Size: 15 2317 32 42 32 2626 Fragments arranged by size: 2626 2317 591 437 135 42 32 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 2166 2887 3699 3945 669 Size: 988 281 228 721 812 246 3079 Fragments arranged by size: 3079 988 812 721 281 246 228 HindIII A'AGCT_T Cuts at: 748 748 Size: 6355 HpaI GTT'AAC Cuts at: 3945 3945 Size: 6355 KpnI G_GTAC'C Cuts at: 3680 3680 Size: 6355 MfeI C'AATT_G Cuts at: 3954 3954 Size: 6355 MluI A'CGCG_T Cuts at: 6231 6231 Size: 6355 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3270 3464 5469 5653 1305 Size: 1965 194 2005 184 2007 Fragments arranged by size: 2007 2005 1965 194 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4533 Fragments arranged by size: 4533 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2223 3387 4313 4672 4831 518 Size: 648 1057 1164 926 359 159 2042 Fragments arranged by size: 2042 1164 1057 926 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2653 3771 4448 5596 5841 1606 Size: 1047 1118 677 1148 245 2120 Fragments arranged by size: 2120 1148 1118 1047 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2671 513 Size: 552 939 667 4197 Fragments arranged by size: 4197 939 667 552 NdeI CA'TA_TG Cuts at: 387 2725 387 Size: 2338 4017 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6355 NotI GC'GGCC_GC Cuts at: 3802 3802 Size: 6355 NspI r_CATG'y Cuts at: 1132 1229 2437 3713 5258 1132 Size: 97 1208 1276 1545 2229 Fragments arranged by size: 2229 1545 1276 1208 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6355 PciI A'CATG_T Cuts at: 2433 5254 2433 Size: 2821 3534 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6355 PfoI T'CCnGG_A Cuts at: 3632 3632 Size: 6355 PmeI GTTT'AAAC Cuts at: 3659 3659 Size: 6355 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4424 4456 5937 5969 4424 Size: 32 1481 32 4810 Fragments arranged by size: 4810 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6355 PsiI TTA'TAA Cuts at: 3925 3925 Size: 6355 PstI C_TGCA'G Cuts at: 830 3707 830 Size: 2877 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6355 SalI G'TCGA_C Cuts at: 1936 3697 1936 Size: 1761 4594 SapI GCTCTTCn'nnn_ Cuts at: 3434 3443 3434 Size: 9 6346 SbfI CC_TGCA'GG Cuts at: 3707 3707 Size: 6355 ScaI AGT'ACT Cuts at: 4603 6315 4603 Size: 1712 4643 SexAI A'CCwGG_T Cuts at: 2564 2564 Size: 6355 SfcI C'TryA_G Cuts at: 826 1045 2632 2880 3375 3703 4838 5519 5710 Size: 219 1587 248 495 328 1135 681 191 Cuts at: 5710 826 Size: 1471 Fragments arranged by size: 1587 1471 1135 681 495 328 248 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6355 SmaI CCC'GGG Cuts at: 1872 3682 1872 Size: 1810 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2510 3406 3561 4459 5360 5622 Size: 197 926 567 896 155 898 901 262 Cuts at: 5622 820 Size: 1553 Fragments arranged by size: 1553 926 901 898 896 567 262 197 155 SnaBI TAC'GTA Cuts at: 493 4146 493 Size: 3653 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6355 SphI G_CATG'C Cuts at: 1132 3713 1132 Size: 2581 3774 SspI AAT'ATT Cuts at: 5 52 4279 5 Size: 47 4227 2081 Fragments arranged by size: 4227 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6355 StyI C'CwwG_G Cuts at: 513 1065 2004 2671 3393 3794 513 Size: 552 939 667 722 401 3074 Fragments arranged by size: 3074 939 722 667 552 401 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2000 2265 4382 4399 4552 4737 5182 Size: 1255 420 265 2117 17 153 185 445 Cuts at: 5182 325 Size: 1498 Fragments arranged by size: 2117 1498 1255 445 420 265 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2898 3015 3379 Size: 274 80 33 51 1443 921 117 364 Cuts at: 3379 4601 6313 96 Size: 1222 1712 138 Fragments arranged by size: 1712 1443 1222 921 364 274 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2490 2522 6315 6347 2490 Size: 32 3793 32 2498 Fragments arranged by size: 3793 2498 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6355 XbaI T'CTAG_A Cuts at: 3691 3691 Size: 6355 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6355 XmaI C'CCGG_G Cuts at: 1870 3680 1870 Size: 1810 4545 XmnI GAAnn'nnTTC Cuts at: 4484 4484 Size: 6355 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4163 276 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI