(Circular) (Six-base) MAPSORT of: pFN21AB9949.seq Check: 2785 from: 1 to: 6301 pFN21AB9949 6301 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3045 3045 Size: 6301 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2566 4111 278 Size: 53 83 186 936 1030 1545 2468 Fragments arranged by size: 2468 1545 1030 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3481 3644 1937 Size: 1544 163 4594 Fragments arranged by size: 4594 1544 163 Acc65I G'GTAC_C Cuts at: 3385 3622 3385 Size: 237 6064 AclI AA'CG_TT Cuts at: 1563 3436 4428 4801 1563 Size: 1873 992 373 3063 Fragments arranged by size: 3063 1873 992 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2280 2823 4364 5748 6016 1632 Size: 648 543 1541 1384 268 1917 Fragments arranged by size: 1917 1541 1384 648 543 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6104 AflIII A'CryG_T Cuts at: 1203 5200 6177 1203 Size: 3997 977 1327 Fragments arranged by size: 3997 1327 977 AgeI A'CCGG_T Cuts at: 5132 5132 Size: 6301 AhdI GACnn_n'nnGTC Cuts at: 5030 5030 Size: 6301 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6301 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6301 AlwNI CAG_nnn'CTG Cuts at: 2080 4044 5616 2080 Size: 1964 1572 2765 Fragments arranged by size: 2765 1964 1572 ApaI G_GGCC'C Cuts at: 3520 3520 Size: 6301 ApaLI G'TGCA_C Cuts at: 4358 5514 4358 Size: 1156 5145 ApoI r'AATT_y Cuts at: 1493 3048 3161 3610 3836 3988 6097 1493 Size: 1555 113 449 226 152 2109 1697 Fragments arranged by size: 2109 1697 1555 449 226 152 113 AseI AT'TA_AT Cuts at: 160 1032 2220 4855 160 Size: 872 1188 2635 1606 Fragments arranged by size: 2635 1606 1188 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 AvaI C'yCGr_G Cuts at: 1870 1943 3626 1870 Size: 73 1683 4545 Fragments arranged by size: 4545 1683 73 BamHI G'GATC_C Cuts at: 3631 3998 3631 Size: 367 5934 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3056 3212 3385 3622 5077 Size: 325 217 627 1269 156 173 237 1455 Cuts at: 5077 618 Size: 1842 Fragments arranged by size: 1842 1455 1269 627 325 237 217 173 156 BanII G_rGCy'C Cuts at: 1391 2995 3520 3620 1391 Size: 1604 525 100 4072 Fragments arranged by size: 4072 1604 525 100 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3566 928 Size: 971 1667 3663 Fragments arranged by size: 3663 1667 971 BbvCI CC'TCA_GC Cuts at: 2010 2010 Size: 6301 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4492 4526 1903 Size: 34 50 34 2471 34 3678 Fragments arranged by size: 3678 2471 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2158 4193 5403 2158 Size: 2035 1210 3056 Fragments arranged by size: 3056 2035 1210 BclI T'GATC_A Cuts at: 1548 2505 1548 Size: 957 5344 BfrBI ATG'CAT Cuts at: 2232 2232 Size: 6301 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4912 136 Size: 107 122 71 631 605 3240 1525 Fragments arranged by size: 3240 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6296 6296 Size: 6301 BlpI GC'TnA_GC Cuts at: 3718 3718 Size: 6301 Bme1580I G_kGCm'C Cuts at: 1790 2202 3195 3215 3520 4362 5518 1790 Size: 412 993 20 305 842 1156 2573 Fragments arranged by size: 2573 1156 993 842 412 305 20 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6301 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2112 4032 4990 Size: 416 53 83 399 24 695 1920 958 Cuts at: 4990 442 Size: 1753 Fragments arranged by size: 1920 1753 958 695 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6301 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2500 3517 4961 1239 Size: 1261 1017 1444 2579 Fragments arranged by size: 2579 1444 1261 1017 Bpu10I CC'TnA_GC Cuts at: 2010 2152 2194 2010 Size: 142 42 6117 Fragments arranged by size: 6117 142 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2539 4426 5291 5589 2539 Size: 1887 865 298 3251 Fragments arranged by size: 3251 1887 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3081 4964 882 Size: 617 1582 1883 2219 Fragments arranged by size: 2219 1883 1582 617 BsaAI yAC'GTr Cuts at: 493 1204 4092 493 Size: 711 2888 2702 Fragments arranged by size: 2888 2702 711 BsaBI GATnn'nnATC Cuts at: 6295 6295 Size: 6301 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2563 4108 4490 5125 Size: 53 83 186 936 1030 1545 382 635 Cuts at: 5125 275 Size: 1451 Fragments arranged by size: 1545 1451 1030 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4734 5132 5406 5553 5964 4734 Size: 398 274 147 411 5071 Fragments arranged by size: 5071 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3336 3366 1178 Size: 30 165 30 1933 30 4113 Fragments arranged by size: 4113 1933 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6301 BseYI C'CCAG_C Cuts at: 2352 3513 5504 6000 2352 Size: 1161 1991 496 2653 Fragments arranged by size: 2653 1991 1161 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3023 1163 Size: 573 1287 4441 Fragments arranged by size: 4441 1287 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3751 4512 5540 1540 Size: 275 186 1750 761 1028 2301 Fragments arranged by size: 2301 1750 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4362 4447 5518 4362 Size: 85 1071 5145 Fragments arranged by size: 5145 1071 85 BsmI GAATG_Cn' Cuts at: 2454 3515 3812 3905 2454 Size: 1061 297 93 4850 Fragments arranged by size: 4850 1061 297 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2995 3195 3215 3520 3620 4362 Size: 399 412 793 200 20 305 100 742 Cuts at: 4362 4447 5518 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 793 742 412 399 305 200 100 85 20 BspEI T'CCGG_A Cuts at: 5964 5964 Size: 6301 BspHI T'CATG_A Cuts at: 4190 5920 4190 Size: 1730 4571 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3045 3656 844 Size: 2201 611 3489 Fragments arranged by size: 3489 2201 611 BsrBI CCG'CTC Cuts at: 1394 1823 4188 1394 Size: 429 2365 3507 Fragments arranged by size: 3507 2365 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2245 3135 4796 4970 65 Size: 1183 997 890 1661 174 1396 Fragments arranged by size: 1661 1396 1183 997 890 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4945 5132 1700 Size: 110 3135 187 2869 Fragments arranged by size: 3135 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6301 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6301 BssSI C'ACGA_G Cuts at: 2061 4361 5373 2061 Size: 2300 1012 2989 Fragments arranged by size: 2989 2300 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6301 BstBI TT'CG_AA Cuts at: 5105 5105 Size: 6301 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6301 BstXI CCAn_nnnn'nTGG Cuts at: 2945 2945 Size: 6301 BstYI r'GATC_y Cuts at: 1972 2318 2948 3631 3998 4383 4400 5852 5938 Size: 346 630 683 367 385 17 1452 86 Cuts at: 5938 5950 6296 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 683 630 385 367 346 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5749 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5562 Fragments arranged by size: 5562 683 56 BtsI GCAGTG_nn' Cuts at: 2414 3817 4611 4631 2414 Size: 1403 794 20 4084 Fragments arranged by size: 4084 1403 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6301 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2746 2781 529 Size: 35 2182 35 4049 Fragments arranged by size: 4049 2182 35 35 DraI TTT'AAA Cuts at: 2181 3605 2181 Size: 1424 4877 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5308 809 Size: 507 3992 1802 Fragments arranged by size: 3992 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3443 3748 4637 Size: 54 1425 50 275 18 1613 305 889 Cuts at: 4637 6266 8 Size: 1629 43 Fragments arranged by size: 1629 1613 1425 889 305 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3748 1537 Size: 275 1936 4090 Fragments arranged by size: 4090 1936 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4231 1353 Size: 2878 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4888 5262 5408 387 Size: 1064 138 3299 374 146 1280 Fragments arranged by size: 3299 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2280 2500 2823 3517 4364 4961 5748 Size: 393 648 220 323 694 847 597 787 Cuts at: 5748 6016 1239 Size: 268 1524 Fragments arranged by size: 1524 847 787 694 648 597 393 323 268 220 EcoRI G'AATT_C Cuts at: 3610 3988 3610 Size: 378 5923 FspI TGC'GCA Cuts at: 4807 4807 Size: 6301 HaeII r_GCGC'y Cuts at: 1678 5448 1678 Size: 3770 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3313 3345 4942 4974 5016 Size: 32 135 32 1741 32 1597 32 42 Cuts at: 5016 5048 1373 Size: 32 2626 Fragments arranged by size: 2626 1741 1597 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3645 3891 669 Size: 988 281 1707 246 3079 Fragments arranged by size: 3079 1707 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6301 HpaI GTT'AAC Cuts at: 3891 3891 Size: 6301 KpnI G_GTAC'C Cuts at: 3389 3626 3389 Size: 237 6064 MfeI C'AATT_G Cuts at: 3075 3365 3447 3900 3075 Size: 290 82 453 5476 Fragments arranged by size: 5476 453 290 82 MluI A'CGCG_T Cuts at: 6177 6177 Size: 6301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2145 2360 2590 5415 5599 1305 Size: 840 215 230 2825 184 2007 Fragments arranged by size: 2825 2007 840 230 215 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4479 Fragments arranged by size: 4479 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2105 2480 2943 4259 4618 4777 518 Size: 648 939 375 463 1316 359 159 2042 Fragments arranged by size: 2042 1316 939 648 463 375 359 159 MspA1I CmG'CkG Cuts at: 1606 2520 2814 3017 3717 4394 5542 5787 1606 Size: 914 294 203 700 677 1148 245 2120 Fragments arranged by size: 2120 1148 914 700 677 294 245 203 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5749 NdeI CA'TA_TG Cuts at: 387 387 Size: 6301 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6301 NotI GC'GGCC_GC Cuts at: 3748 3748 Size: 6301 NsiI A_TGCA'T Cuts at: 2234 2234 Size: 6301 NspI r_CATG'y Cuts at: 1132 1229 2232 3659 5204 1132 Size: 97 1003 1427 1545 2229 Fragments arranged by size: 2229 1545 1427 1003 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6301 PciI A'CATG_T Cuts at: 5200 5200 Size: 6301 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6301 PfoI T'CCnGG_A Cuts at: 2906 2906 Size: 6301 PmeI GTTT'AAAC Cuts at: 3605 3605 Size: 6301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4370 4402 5883 5915 4370 Size: 32 1481 32 4756 Fragments arranged by size: 4756 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6301 PsiI TTA'TAA Cuts at: 3871 3871 Size: 6301 PspOMI G'GGCC_C Cuts at: 3516 3516 Size: 6301 PstI C_TGCA'G Cuts at: 830 3653 830 Size: 2823 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6301 PvuII CAG'CTG Cuts at: 2520 2814 3017 2520 Size: 294 203 5804 Fragments arranged by size: 5804 294 203 SalI G'TCGA_C Cuts at: 1936 3643 1936 Size: 1707 4594 SbfI CC_TGCA'GG Cuts at: 3653 3653 Size: 6301 ScaI AGT'ACT Cuts at: 4549 6261 4549 Size: 1712 4589 SfcI C'TryA_G Cuts at: 826 1045 3295 3649 4784 5465 5656 826 Size: 219 2250 354 1135 681 191 1471 Fragments arranged by size: 2250 1471 1135 681 354 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6301 SmaI CCC'GGG Cuts at: 1872 3628 1872 Size: 1756 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2554 4405 5306 5568 820 Size: 197 926 611 1851 901 262 1553 Fragments arranged by size: 1851 1553 926 901 611 262 197 SnaBI TAC'GTA Cuts at: 493 4092 493 Size: 3599 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6301 SphI G_CATG'C Cuts at: 1132 3659 1132 Size: 2527 3774 SspI AAT'ATT Cuts at: 5 52 4225 5 Size: 47 4173 2081 Fragments arranged by size: 4173 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6301 StyI C'CwwG_G Cuts at: 513 1065 3061 3740 513 Size: 552 1996 679 3074 Fragments arranged by size: 3074 1996 679 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3074 4328 4345 4498 4683 5128 325 Size: 1255 1494 1254 17 153 185 445 1498 Fragments arranged by size: 1498 1494 1255 1254 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4547 6259 96 Size: 274 80 33 51 1443 2570 1712 138 Fragments arranged by size: 2570 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3336 3368 6261 6293 3336 Size: 32 2893 32 3344 Fragments arranged by size: 3344 2893 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6301 XbaI T'CTAG_A Cuts at: 3637 3637 Size: 6301 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3223 3223 Size: 6301 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6301 XmaI C'CCGG_G Cuts at: 1870 3626 1870 Size: 1756 4545 XmnI GAAnn'nnTTC Cuts at: 2789 4430 2789 Size: 1641 4660 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2564 4109 276 Size: 53 83 186 936 1030 1545 2468 Fragments arranged by size: 2468 1545 1030 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI