################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI DraI DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HgaI HincII HindII HindIII HpaI HphI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboII MfeI MluI MluNI MroI MscI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AcyI 157 GGCGCC G[GA]^CG[CT]C AlwI 342 GGATC GGATC(4/5) AvaI 109 CCCGGG C^[CT]CG[GA]G AvaII 151 GGACC G^G[AT]CC BanI 157 GGCGCC G^G[CT][GA]CC BbeI 157 GGCGCC GGCGC^C BglI 33 GCCGTTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 165 GGGCAC G[AGT]GC[ACT]^C BpmI 184 CTGGAG CTGGAG(16/14) BsaHI 157 GGCGCC G[GA]^CG[CT]C BsiEI 70 CGGCCG CG[GA][CT]^CG BsiYI 73 CCGCCTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 73 CCGCCTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsoBI 109 CCCGGG C^[CT]CG[GA]G Bsp1286I 165 GGGCAC G[AGT]GC[ACT]^C BsrDI 358 CATTGC GCAATG(2/0) BsrFI 68 GCCGGC [GA]^CCGG[CT] BssHII 91 GCGCGC G^CGCGC BstF5I 48 CATCC GGATG(2/0) BstYI 342 GGATCT [GA]^GATC[CT] Cfr10I 68 GCCGGC [GA]^CCGG[CT] CpoI 150 CGGACCG CG^G[AT]CCG DpnII 343 GATC ^GATC DraII 271 AGGGCCT [GA]G^G[ACGT]CC[CT] EagI 70 CGGCCG C^GGCCG EclXI 70 CGGCCG C^GGCCG Eco52I 70 CGGCCG C^GGCCG EcoO109I 271 AGGGCCT [GA]G^G[ACGT]CC[CT] FokI 48 CATCC GGATG(9/13) HaeII 157 GGCGCC [GA]GCGC^[CT] Hin1I 157 GGCGCC G[GA]^CG[CT]C HinfI 244 GAATC G^A[ACGT]TC KasI 157 GGCGCC G^GCGCC MboI 343 GATC ^GATC MflI 342 GGATCT [GA]^GATC[CT] MseI 278 TTAA T^TAA MspA1I 147 CAGCGG C[AC]G^C[GT]G NaeI 68 GCCGGC GCC^GGC NarI 157 GGCGCC GG^CGCC NdeII 343 GATC ^GATC NgoMIV 68 GCCGGC G^CCGGC NlaIII 96 CATG CATG^ NspI 95 GCATGC [GA]CATG^[CT] PstI 239 CTGCAG CTGCA^G RsrII 150 CGGACCG CG^G[AT]CCG Sau3AI 343 GATC ^GATC SfaNI 47 GCATC GCATC(5/9) SfcI 239 CTGCAG C^T[GA][CT]AG SfoI 157 GGCGCC GGC^GCC SmaI 109 CCCGGG CCC^GGG SphI 95 GCATGC GCATG^C TfiI 244 GAATC G^A[AT]TC Tru9I 278 TTAA T^TAA XhoII 342 GGATCT [GA]^GATC[CT] XmaCI 109 CCCGGG C^CCGGG XmaI 109 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AfaI 87 GTAC GT^AC 326 GTAC AluI 188 AGCT AG^CT 253 AGCT BsrI 288 ACTGG ACTGG(1/-1) 338 ACTGG BstNI 161 CCTGG CC^[AT]GG 183 CCTGG DdeI 268 CTGAG C^T[ACGT]AG 293 CTCAG EaeI 70 CGGCCG [CT]^GGCC[GA] 143 CGGCCA EcoRII 161 CCTGG ^CC[AT]GG 183 CCTGG MvaI 161 CCTGG CC^[AT]GG 183 CCTGG NlaIV 30 GGGGCC GG[ACGT]^[ACGT]CC 157 GGCGCC RsaI 87 GTAC GT^AC 326 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AccII AciI BbvI BcnI BsaJI BssKI BstUI Cac8I CfoI Cfr13I Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspI MvnI MwoI NciI Sau96I ScrFI TseI TspRI