################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AocI ApoI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbeI BbrPI BciVI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BsiWI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI EclXI Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FseI FspI HincII HindII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI Tru9I Tsp509I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 637 CGCG CG^CG AcyI 73 GGCGTC G[GA]^CG[CT]C AflIII 1232 ACACGT A^C[GA][CT]GT AgeI 1025 ACCGGT A^CCGGT AhdI 1228 GACTACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 664 GTGCAC G^TGCAC Aor51HI 946 AGCGCT AGC^GCT ApaI 849 GGGCCC GGGCC^C ApaLI 664 GTGCAC G^TGCAC AspEI 1228 GACTACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 207 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 196 GTCTTC GAAGAC(2/6) BcgI 185 GCAACAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 326 TGATCA T^GATCA BglII 578 AGATCT A^GATCT BpuAI 196 GTCTTC GAAGAC(2/6) BsaHI 73 GGCGTC G[GA]^CG[CT]C BsaWI 1025 ACCGGT [AT]^CCGG[AT] BsgI 1260 CTGCAC GTGCAG(16/14) BsiEI 369 CGACCG CG[GA][CT]^CG BsmBI 900 CGTCTC CGTCTC(1/5) BsmI 759 GAATGC GAATGC(1/-1) Bsp1407I 155 TGTACA T^GTACA BsrDI 360 CATTGC GCAATG(2/0) BsrFI 1025 ACCGGT [GA]^CCGG[CT] BsrGI 155 TGTACA T^GTACA BstUI 637 CGCG CG^CG BstXI 812 CCATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 1025 ACCGGT [GA]^CCGG[CT] Eam1105I 1228 GACTACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 946 AGCGCT AGC^GCT FbaI 326 TGATCA T^GATCA HaeII 946 AGCGCT [GA]GCGC^[CT] HgaI 74 GCGTC GACGC(5/10) Hin1I 73 GGCGTC G[GA]^CG[CT]C HindIII 1392 AAGCTT A^AGCTT MaeII 1234 ACGT A^CGT MvnI 637 CGCG CG^CG NcoI 395 CCATGG C^CATGG PflFI 207 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1025 ACCGGT A^CCGGT SapI 1071 GCTCTTC GCTCTTC(1/4) SexAI 134 ACCTGGT A^CC[AT]GGT SmaI 1482 CCCGGG CCC^GGG SmlI 1222 CTTGAG C^T[CT][GA]AG SphI 341 GCATGC GCATG^C SspBI 155 TGTACA T^GTACA TfiI 1341 GATTC G^A[AT]TC Tth111I 207 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1482 CCCGGG C^CCGGG XmaI 1482 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 445 GTCTAC GT^[AC][GT]AC 778 GTCTAC AlwI 278 GGATC GGATC(4/5) 1091 GGATC AlwNI 1144 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1531 CAGAGGCTG BalI 641 TGGCCA TGG^CCA 1397 TGGCCA BanI 545 GGCACC G^G[CT][GA]CC 829 GGTGCC BbvCI 18 CCTCAGC CCTCAGC(-5/-2) 180 CCTCAGC BpmI 723 CTCCAG CTGGAG(16/14) 913 CTGGAG BsmAI 916 GAGAC GTCTC(1/5) 901 GTCTC BspMI 320 ACCTGC ACCTGC(4/8) 1135 GCAGGT BstEII 147 GGTCACC G^GT[ACGT]ACC 840 GGTCACC BstPI 147 GGTCACC G^GT[ACGT]ACC 840 GGTCACC BstYI 278 GGATCT [GA]^GATC[CT] 578 AGATCT CfoI 947 GCGC GCG^C 1126 GCGC DsaI 395 CCATGG C^C[GA][CT]GG 503 CCGTGG EcoO65I 147 GGTCACC G^GT[ACGT]ACC 840 GGTCACC EcoT14I 395 CCATGG C^C[AT][AT]GG 1373 CCTTGG HhaI 947 GCGC GCG^C 1126 GCGC HinP1I 947 GCGC G^CGC 1126 GCGC MflI 278 GGATCT [GA]^GATC[CT] 578 AGATCT MluNI 641 TGGCCA TGG^CCA 1397 TGGCCA MscI 641 TGGCCA TGG^CCA 1397 TGGCCA MslI 609 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 813 CATCCTGGTG MspA1I 21 CAGCTG C[AC]G^C[GT]G 1185 CAGCTG NspI 341 GCATGC [GA]CATG^[CT] 1010 GCATGT PstI 24 CTGCAG CTGCA^G 1537 CTGCAG PvuII 21 CAGCTG CAG^CTG 1185 CAGCTG SacI 258 GAGCTC GAGCT^C 1284 GAGCTC SfcI 24 CTGCAG C^T[GA][CT]AG 1537 CTGCAG StyI 395 CCATGG C^C[AT][AT]GG 1373 CCTTGG XhoII 278 GGATCT [GA]^GATC[CT] 578 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AfaI AluI AspHI AvaI AvaII BanII BbvI BcnI BglI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp45I TspRI TthHB8I