(Circular) (Six-base) MAPSORT of: pFN21AE9459.seq Check: 6445 from: 1 to: 6292 pFN21AE9459 6292 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3131 3131 Size: 6292 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4102 278 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2451 2784 3635 1937 Size: 514 333 851 4594 Fragments arranged by size: 4594 851 514 333 Acc65I G'GTAC_C Cuts at: 3613 3613 Size: 6292 AclI AA'CG_TT Cuts at: 1563 4419 4792 1563 Size: 2856 373 3063 Fragments arranged by size: 3063 2856 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2188 2733 3064 3172 3384 4355 5739 6007 Size: 556 545 331 108 212 971 1384 268 Cuts at: 6007 1632 Size: 1917 Fragments arranged by size: 1917 1384 971 556 545 331 268 212 108 AfeI AGC'GCT Cuts at: 2953 2953 Size: 6292 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6095 AflIII A'CryG_T Cuts at: 1203 3237 5191 6168 1203 Size: 2034 1954 977 1327 Fragments arranged by size: 2034 1954 1327 977 AgeI A'CCGG_T Cuts at: 3030 5123 3030 Size: 2093 4199 AhdI GACnn_n'nnGTC Cuts at: 3238 5021 3238 Size: 1783 4509 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6292 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6292 AlwNI CAG_nnn'CTG Cuts at: 3154 3541 4035 5607 3154 Size: 387 494 1572 3839 Fragments arranged by size: 3839 1572 494 387 ApaI G_GGCC'C Cuts at: 2858 2858 Size: 6292 ApaLI G'TGCA_C Cuts at: 2669 4349 5505 2669 Size: 1680 1156 3456 Fragments arranged by size: 3456 1680 1156 ApoI r'AATT_y Cuts at: 1493 3601 3827 3979 6088 1493 Size: 2108 226 152 2109 1697 Fragments arranged by size: 2109 2108 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4846 160 Size: 872 3814 1606 Fragments arranged by size: 3814 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 AvaI C'yCGr_G Cuts at: 1870 1943 2357 2911 3487 3617 1870 Size: 73 414 554 576 130 4545 Fragments arranged by size: 4545 576 554 414 130 73 BamHI G'GATC_C Cuts at: 3622 3989 3622 Size: 367 5925 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2550 2834 3613 5068 618 Size: 325 217 627 763 284 779 1455 1842 Fragments arranged by size: 1842 1455 779 763 627 325 284 217 BanII G_rGCy'C Cuts at: 1391 2267 2858 3293 3611 1391 Size: 876 591 435 318 4072 Fragments arranged by size: 4072 876 591 435 318 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2194 928 Size: 971 295 5026 Fragments arranged by size: 5026 971 295 BbvCI CC'TCA_GC Cuts at: 2024 2186 2024 Size: 162 6130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2179 2213 4483 4517 1903 Size: 34 242 34 2270 34 3678 Fragments arranged by size: 3678 2270 242 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4184 5394 4184 Size: 1210 5082 BclI T'GATC_A Cuts at: 1548 2331 1548 Size: 783 5509 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2882 3157 3416 Size: 107 122 71 631 605 1210 275 259 Cuts at: 3416 3513 4903 136 Size: 97 1390 1525 Fragments arranged by size: 1525 1390 1210 631 605 275 259 122 107 97 71 BglII A'GATC_T Cuts at: 2583 6287 2583 Size: 3704 2588 BlpI GC'TnA_GC Cuts at: 3709 3709 Size: 6292 Bme1580I G_kGCm'C Cuts at: 1790 2596 2673 2858 3162 4353 5509 1790 Size: 806 77 185 304 1191 1156 2573 Fragments arranged by size: 2573 1191 1156 806 304 185 77 BmgBI CAC'GTC Cuts at: 1318 3240 1318 Size: 1922 4370 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2086 3020 4023 Size: 416 53 83 399 24 669 934 1003 Cuts at: 4023 4981 442 Size: 958 1753 Fragments arranged by size: 1753 1003 958 934 669 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6292 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2713 2939 4952 1239 Size: 1474 226 2013 2579 Fragments arranged by size: 2579 2013 1474 226 Bpu10I CC'TnA_GC Cuts at: 2024 2186 2024 Size: 162 6130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3248 4417 5282 5580 3248 Size: 1169 865 298 3960 Fragments arranged by size: 3960 1169 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4955 882 Size: 617 3456 2219 Fragments arranged by size: 3456 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4083 493 Size: 711 2879 2702 Fragments arranged by size: 2879 2702 711 BsaBI GATnn'nnATC Cuts at: 6286 6286 Size: 6292 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2079 4099 4481 5116 Size: 53 83 186 936 546 2020 382 635 Cuts at: 5116 275 Size: 1451 Fragments arranged by size: 2020 1451 936 635 546 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3030 4725 5123 5397 5544 5955 3030 Size: 1695 398 274 147 411 3367 Fragments arranged by size: 3367 1695 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6067 Fragments arranged by size: 6067 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2008 2072 2075 2168 2309 2711 2734 3461 Size: 822 64 3 93 141 402 23 727 Cuts at: 3461 1186 Size: 4017 Fragments arranged by size: 4017 822 727 402 141 93 64 23 3 BseYI C'CCAG_C Cuts at: 5495 5991 5495 Size: 496 5796 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3250 1163 Size: 573 1514 4205 Fragments arranged by size: 4205 1514 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2377 3742 4503 5531 1540 Size: 275 186 376 1365 761 1028 2301 Fragments arranged by size: 2301 1365 1028 761 376 275 186 BsiHKAI G_wGCw'C Cuts at: 2267 2673 3293 4353 4438 5509 2267 Size: 406 620 1060 85 1071 3050 Fragments arranged by size: 3050 1071 1060 620 406 85 BsmI GAATG_Cn' Cuts at: 2770 3803 3896 2770 Size: 1033 93 5166 Fragments arranged by size: 5166 1033 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2911 2911 Size: 6292 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2267 2596 2673 2858 3162 3293 3611 Size: 399 477 329 77 185 304 131 318 Cuts at: 3611 4353 4438 5509 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 477 399 329 318 304 185 131 85 77 BspEI T'CCGG_A Cuts at: 5955 5955 Size: 6292 BspHI T'CATG_A Cuts at: 4181 5911 4181 Size: 1730 4562 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2334 3131 3647 844 Size: 1490 797 516 3489 Fragments arranged by size: 3489 1490 797 516 BsrBI CCG'CTC Cuts at: 1394 1823 4179 1394 Size: 429 2356 3507 Fragments arranged by size: 3507 2356 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2364 4787 4961 65 Size: 1183 1116 2423 174 1396 Fragments arranged by size: 2423 1396 1183 1116 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3030 4936 5123 1700 Size: 110 1220 1906 187 2869 Fragments arranged by size: 2869 1906 1220 187 110 BsrGI T'GTAC_A Cuts at: 96 2160 96 Size: 2064 4228 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6292 BssSI C'ACGA_G Cuts at: 4352 5364 4352 Size: 1012 5280 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6292 BstBI TT'CG_AA Cuts at: 5096 5096 Size: 6292 BstEII G'GTnAC_C Cuts at: 1184 2152 2845 1184 Size: 968 693 4631 Fragments arranged by size: 4631 968 693 BstXI CCAn_nnnn'nTGG Cuts at: 2824 2824 Size: 6292 BstYI r'GATC_y Cuts at: 1972 2283 2583 3622 3989 4374 4391 5843 5929 Size: 311 300 1039 367 385 17 1452 86 Cuts at: 5929 5941 6287 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1039 385 367 346 311 300 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2400 2508 513 Size: 552 1335 108 4297 Fragments arranged by size: 4297 1335 552 108 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2083 2857 487 Size: 683 56 857 774 3922 Fragments arranged by size: 3922 857 774 683 56 BtsI GCAGTG_nn' Cuts at: 3126 3262 3808 4602 4622 3126 Size: 136 546 794 20 4796 Fragments arranged by size: 4796 794 546 136 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6292 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6257 DraI TTT'AAA Cuts at: 3596 3596 Size: 6292 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5299 809 Size: 507 3983 1802 Fragments arranged by size: 3983 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2646 2790 3402 Size: 54 1425 50 275 18 816 144 612 Cuts at: 3402 3739 4628 6257 8 Size: 337 889 1629 43 Fragments arranged by size: 1629 1425 889 816 612 337 275 144 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3739 1537 Size: 275 1927 4090 Fragments arranged by size: 4090 1927 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2603 2752 3083 4222 1353 Size: 1250 149 331 1139 3423 Fragments arranged by size: 3423 1250 1139 331 149 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4879 5253 5399 387 Size: 1064 138 3290 374 146 1280 Fragments arranged by size: 3290 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2265 3291 2265 Size: 1026 5266 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2188 2713 2733 2939 3064 3172 3384 Size: 393 556 525 20 206 125 108 212 Cuts at: 3384 4355 4952 5739 6007 1239 Size: 971 597 787 268 1524 Fragments arranged by size: 1524 971 787 597 556 525 393 268 212 206 125 108 20 EcoO109I rG'GnC_Cy Cuts at: 2854 2855 2884 2854 Size: 1 29 6262 Fragments arranged by size: 6262 29 1 EcoRI G'AATT_C Cuts at: 3601 3979 3601 Size: 378 5914 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2218 2218 Size: 6292 FspI TGC'GCA Cuts at: 4798 4798 Size: 6292 HaeII r_GCGC'y Cuts at: 1678 2955 5439 1678 Size: 1277 2484 2531 Fragments arranged by size: 2531 2484 1277 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3224 3256 4933 4965 5007 Size: 32 135 32 1652 32 1677 32 42 Cuts at: 5007 5039 1373 Size: 32 2626 Fragments arranged by size: 2626 1677 1652 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3636 3882 669 Size: 988 281 1698 246 3079 Fragments arranged by size: 3079 1698 988 281 246 HindIII A'AGCT_T Cuts at: 748 3397 748 Size: 2649 3643 HpaI GTT'AAC Cuts at: 3882 3882 Size: 6292 KpnI G_GTAC'C Cuts at: 3617 3617 Size: 6292 MfeI C'AATT_G Cuts at: 3891 3891 Size: 6292 MluI A'CGCG_T Cuts at: 6168 6168 Size: 6292 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2244 3114 5406 5590 1305 Size: 939 870 2292 184 2007 Fragments arranged by size: 2292 2007 939 870 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2648 3404 10 Size: 54 1425 343 816 756 2898 Fragments arranged by size: 2898 1425 816 756 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2618 2822 4250 4609 4768 518 Size: 648 1452 204 1428 359 159 2042 Fragments arranged by size: 2042 1452 1428 648 359 204 159 MspA1I CmG'CkG Cuts at: 1606 2028 3192 3708 4385 5533 5778 1606 Size: 422 1164 516 677 1148 245 2120 Fragments arranged by size: 2120 1164 1148 677 516 422 245 NcoI C'CATG_G Cuts at: 513 1065 2400 513 Size: 552 1335 4405 Fragments arranged by size: 4405 1335 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6292 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6292 NotI GC'GGCC_GC Cuts at: 3739 3739 Size: 6292 NspI r_CATG'y Cuts at: 1132 1229 2350 3019 3650 5195 1132 Size: 97 1121 669 631 1545 2229 Fragments arranged by size: 2229 1545 1121 669 631 97 PasI CC'CwG_GG Cuts at: 1348 3154 1348 Size: 1806 4486 PciI A'CATG_T Cuts at: 5191 5191 Size: 6292 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6292 PmeI GTTT'AAAC Cuts at: 3596 3596 Size: 6292 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4361 4393 5874 5906 4361 Size: 32 1481 32 4747 Fragments arranged by size: 4747 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6292 PsiI TTA'TAA Cuts at: 3862 3862 Size: 6292 PspOMI G'GGCC_C Cuts at: 2854 2854 Size: 6292 PstI C_TGCA'G Cuts at: 830 2033 3546 3644 830 Size: 1203 1513 98 3478 Fragments arranged by size: 3478 1513 1203 98 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6292 PvuII CAG'CTG Cuts at: 2028 3192 2028 Size: 1164 5128 SacI G_AGCT'C Cuts at: 2267 3293 2267 Size: 1026 5266 SalI G'TCGA_C Cuts at: 1936 3634 1936 Size: 1698 4594 SapI GCTCTTCn'nnn_ Cuts at: 3083 3083 Size: 6292 SbfI CC_TGCA'GG Cuts at: 3644 3644 Size: 6292 ScaI AGT'ACT Cuts at: 4540 6252 4540 Size: 1712 4580 SexAI A'CCwGG_T Cuts at: 2139 2139 Size: 6292 SfcI C'TryA_G Cuts at: 826 1045 2029 3542 3640 4775 5456 5647 826 Size: 219 984 1513 98 1135 681 191 1471 Fragments arranged by size: 1513 1471 1135 984 681 219 191 98 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6292 SmaI CCC'GGG Cuts at: 1872 3489 3619 1872 Size: 1617 130 4545 Fragments arranged by size: 4545 1617 130 SmlI C'TyrA_G Cuts at: 820 1017 1943 3227 4396 5297 5559 820 Size: 197 926 1284 1169 901 262 1553 Fragments arranged by size: 1553 1284 1169 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4083 493 Size: 3590 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6292 SphI G_CATG'C Cuts at: 1132 2350 3650 1132 Size: 1218 1300 3774 Fragments arranged by size: 3774 1300 1218 SspI AAT'ATT Cuts at: 5 52 4216 5 Size: 47 4164 2081 Fragments arranged by size: 4164 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6292 StyI C'CwwG_G Cuts at: 513 1065 2400 3378 3731 513 Size: 552 1335 978 353 3074 Fragments arranged by size: 3074 1335 978 552 353 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2105 3292 4319 4336 4489 4674 5119 Size: 1255 525 1187 1027 17 153 185 445 Cuts at: 5119 325 Size: 1498 Fragments arranged by size: 1498 1255 1187 1027 525 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2160 3367 4538 Size: 274 80 33 51 1443 183 1207 1171 Cuts at: 4538 6250 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1207 1171 274 183 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2275 2307 3468 3500 6252 6284 2275 Size: 32 1161 32 2752 32 2283 Fragments arranged by size: 2752 2283 1161 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2215 1316 Size: 899 5393 XbaI T'CTAG_A Cuts at: 3628 3628 Size: 6292 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6292 XmaI C'CCGG_G Cuts at: 1870 3487 3617 1870 Size: 1617 130 4545 Fragments arranged by size: 4545 1617 130 XmnI GAAnn'nnTTC Cuts at: 4421 4421 Size: 6292 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4100 276 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI