(Circular) (Six-base) MAPSORT of: pFN21AE1372.seq Check: 9027 from: 1 to: 6439 pFN21AE1372 6439 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4249 278 Size: 53 83 186 936 2713 2468 Fragments arranged by size: 2713 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3782 1937 Size: 1845 4594 Acc65I G'GTAC_C Cuts at: 3760 3760 Size: 6439 AclI AA'CG_TT Cuts at: 1563 4566 4939 1563 Size: 3003 373 3063 Fragments arranged by size: 3063 3003 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2470 2557 2948 3015 3384 4502 5886 6154 Size: 838 87 391 67 369 1118 1384 268 Cuts at: 6154 1632 Size: 1917 Fragments arranged by size: 1917 1384 1118 838 391 369 268 87 67 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6242 AflIII A'CryG_T Cuts at: 1203 5338 6315 1203 Size: 4135 977 1327 Fragments arranged by size: 4135 1327 977 AgeI A'CCGG_T Cuts at: 5270 5270 Size: 6439 AhdI GACnn_n'nnGTC Cuts at: 5168 5168 Size: 6439 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6439 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6439 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2204 2236 2204 Size: 32 6407 AlwNI CAG_nnn'CTG Cuts at: 2239 3323 4182 5754 2239 Size: 1084 859 1572 2924 Fragments arranged by size: 2924 1572 1084 859 ApaI G_GGCC'C Cuts at: 3159 3538 3159 Size: 379 6060 ApaLI G'TGCA_C Cuts at: 4496 5652 4496 Size: 1156 5283 ApoI r'AATT_y Cuts at: 1493 2114 3431 3748 3974 4126 6235 1493 Size: 621 1317 317 226 152 2109 1697 Fragments arranged by size: 2109 1697 1317 621 317 226 152 AseI AT'TA_AT Cuts at: 160 1032 4993 160 Size: 872 3961 1606 Fragments arranged by size: 3961 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6439 AvaI C'yCGr_G Cuts at: 1870 1943 2423 3764 1870 Size: 73 480 1341 4545 Fragments arranged by size: 4545 1341 480 73 BamHI G'GATC_C Cuts at: 3502 3769 4136 3502 Size: 267 367 5805 Fragments arranged by size: 5805 367 267 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2524 2787 3298 3760 5215 Size: 325 217 627 737 263 511 462 1455 Cuts at: 5215 618 Size: 1842 Fragments arranged by size: 1842 1455 737 627 511 462 325 263 217 BanII G_rGCy'C Cuts at: 1391 2752 3003 3063 3135 3146 3159 3538 3758 Size: 1361 251 60 72 11 13 379 220 Cuts at: 3758 1391 Size: 4072 Fragments arranged by size: 4072 1361 379 251 220 72 60 13 11 BbeI G_GCGC'C Cuts at: 2791 2791 Size: 6439 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3318 928 Size: 971 1419 4049 Fragments arranged by size: 4049 1419 971 BbvCI CC'TCA_GC Cuts at: 2042 2042 Size: 6439 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4630 4664 1903 Size: 34 50 34 2609 34 3678 Fragments arranged by size: 3678 2609 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4331 5541 4331 Size: 1210 5229 BclI T'GATC_A Cuts at: 1548 1548 Size: 6439 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5050 136 Size: 107 122 71 631 605 3378 1525 Fragments arranged by size: 3378 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6434 6434 Size: 6439 BlpI GC'TnA_GC Cuts at: 3856 3856 Size: 6439 Bme1580I G_kGCm'C Cuts at: 1790 3159 3538 4500 5656 1790 Size: 1369 379 962 1156 2573 Fragments arranged by size: 2573 1369 1156 962 379 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6439 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2799 2998 4170 Size: 416 53 83 399 24 1382 199 1172 Cuts at: 4170 5128 442 Size: 958 1753 Fragments arranged by size: 1753 1382 1172 958 416 399 199 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6439 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2692 2692 Size: 6439 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2610 2756 3413 3640 5099 1239 Size: 1371 146 657 227 1459 2579 Fragments arranged by size: 2579 1459 1371 657 227 146 Bpu10I CC'TnA_GC Cuts at: 2042 3140 2042 Size: 1098 5341 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3607 4564 5429 5727 3607 Size: 957 865 298 4319 Fragments arranged by size: 4319 957 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2861 3677 5102 882 Size: 617 1362 816 1425 2219 Fragments arranged by size: 2219 1425 1362 816 617 BsaAI yAC'GTr Cuts at: 493 1204 4230 493 Size: 711 3026 2702 Fragments arranged by size: 3026 2702 711 BsaBI GATnn'nnATC Cuts at: 6433 6433 Size: 6439 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2788 4246 4628 5263 Size: 53 83 186 936 1255 1458 382 635 Cuts at: 5263 275 Size: 1451 Fragments arranged by size: 1458 1451 1255 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2744 4872 5270 5544 5691 6102 2744 Size: 2128 398 274 147 411 3081 Fragments arranged by size: 3081 2128 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2204 2234 1178 Size: 30 165 30 801 30 5383 Fragments arranged by size: 5383 801 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2681 2912 3002 1186 Size: 844 651 231 90 4623 Fragments arranged by size: 4623 844 651 231 90 BseYI C'CCAG_C Cuts at: 2084 2828 3046 3070 3134 3146 3242 3316 5642 Size: 744 218 24 64 12 96 74 2326 Cuts at: 5642 6138 2084 Size: 496 2385 Fragments arranged by size: 2385 2326 744 496 218 96 74 64 24 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2927 1163 Size: 573 1191 4675 Fragments arranged by size: 4675 1191 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3889 4650 5678 1540 Size: 275 186 1888 761 1028 2301 Fragments arranged by size: 2301 1888 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4500 4585 5656 4500 Size: 85 1071 5283 Fragments arranged by size: 5283 1071 85 BsmI GAATG_Cn' Cuts at: 3329 3950 4043 3329 Size: 621 93 5725 Fragments arranged by size: 5725 621 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2752 3003 3063 3135 3146 3159 3538 Size: 399 962 251 60 72 11 13 379 Cuts at: 3538 3758 4500 4585 5656 1391 Size: 220 742 85 1071 2174 Fragments arranged by size: 2174 1071 962 742 399 379 251 220 85 72 60 13 11 BspEI T'CCGG_A Cuts at: 2744 6102 2744 Size: 3358 3081 BspHI T'CATG_A Cuts at: 4328 6058 4328 Size: 1730 4709 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2177 2949 3794 844 Size: 1333 772 845 3489 Fragments arranged by size: 3489 1333 845 772 BsrBI CCG'CTC Cuts at: 1394 1823 4326 1394 Size: 429 2503 3507 Fragments arranged by size: 3507 2503 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2290 4934 5108 65 Size: 1183 1042 2644 174 1396 Fragments arranged by size: 2644 1396 1183 1042 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2322 5083 5270 1700 Size: 110 512 2761 187 2869 Fragments arranged by size: 2869 2761 512 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6439 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6439 BssSI C'ACGA_G Cuts at: 4499 5511 4499 Size: 1012 5427 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6439 BstBI TT'CG_AA Cuts at: 5243 5243 Size: 6439 BstEII G'GTnAC_C Cuts at: 1184 3338 3700 1184 Size: 2154 362 3923 Fragments arranged by size: 3923 2154 362 BstXI CCAn_nnnn'nTGG Cuts at: 2285 2285 Size: 6439 BstYI r'GATC_y Cuts at: 1972 2403 3117 3502 3769 4136 4521 4538 5990 Size: 431 714 385 267 367 385 17 1452 Cuts at: 5990 6076 6088 6434 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 714 431 385 385 367 346 267 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2189 3294 3344 3529 2189 Size: 1105 50 185 5099 Fragments arranged by size: 5099 1105 185 50 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5887 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5700 Fragments arranged by size: 5700 683 56 BtsI GCAGTG_nn' Cuts at: 2265 2395 3604 3955 4749 4769 2265 Size: 130 1209 351 794 20 3935 Fragments arranged by size: 3935 1209 794 351 130 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6439 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6404 DraI TTT'AAA Cuts at: 3743 3743 Size: 6439 DraIII CAC_nnn'GTG Cuts at: 3257 3257 Size: 6439 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5446 809 Size: 507 4130 1802 Fragments arranged by size: 4130 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3480 3886 4775 Size: 54 1425 50 275 18 1650 406 889 Cuts at: 4775 6404 8 Size: 1629 43 Fragments arranged by size: 1650 1629 1425 889 406 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3886 1537 Size: 275 2074 4090 Fragments arranged by size: 4090 2074 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2822 2991 3360 4369 1353 Size: 1469 169 369 1009 3423 Fragments arranged by size: 3423 1469 1009 369 169 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5026 5400 5546 387 Size: 1064 138 3437 374 146 1280 Fragments arranged by size: 3437 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2470 2557 2610 2756 2948 3015 3384 Size: 393 838 87 53 146 192 67 369 Cuts at: 3384 3413 3640 4502 5099 5886 6154 1239 Size: 29 227 862 597 787 268 1524 Fragments arranged by size: 1524 862 838 787 597 393 369 268 227 192 146 87 67 53 29 EcoNI CCTnn'n_nnAGG Cuts at: 3482 3482 Size: 6439 EcoO109I rG'GnC_Cy Cuts at: 3155 3534 3535 3155 Size: 379 1 6059 Fragments arranged by size: 6059 379 1 EcoRI G'AATT_C Cuts at: 3748 4126 3748 Size: 378 6061 FspI TGC'GCA Cuts at: 4945 4945 Size: 6439 HaeII r_GCGC'y Cuts at: 1678 2039 2791 5586 1678 Size: 361 752 2795 2531 Fragments arranged by size: 2795 2531 752 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3386 3418 3557 3589 5080 Size: 32 135 32 1814 32 139 32 1491 Cuts at: 5080 5112 5154 5186 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1814 1491 139 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3783 4029 669 Size: 988 281 1845 246 3079 Fragments arranged by size: 3079 1845 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6439 HpaI GTT'AAC Cuts at: 4029 4029 Size: 6439 KasI G'GCGC_C Cuts at: 2787 2787 Size: 6439 KpnI G_GTAC'C Cuts at: 3764 3764 Size: 6439 MfeI C'AATT_G Cuts at: 4038 4038 Size: 6439 MluI A'CGCG_T Cuts at: 6315 6315 Size: 6439 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2247 2355 5553 5737 1305 Size: 942 108 3198 184 2007 Fragments arranged by size: 3198 2007 942 184 108 MscI TGG'CCA Cuts at: 10 64 1489 1832 3482 10 Size: 54 1425 343 1650 2967 Fragments arranged by size: 2967 1650 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2283 4397 4756 4915 518 Size: 648 1117 2114 359 159 2042 Fragments arranged by size: 2114 2042 1117 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2776 3081 3114 3320 3594 3855 4532 5680 Size: 1170 305 33 206 274 261 677 1148 Cuts at: 5680 5925 1606 Size: 245 2120 Fragments arranged by size: 2120 1170 1148 677 305 274 261 245 206 33 NaeI GCC'GGC Cuts at: 2324 2324 Size: 6439 NarI GG'CG_CC Cuts at: 2788 2788 Size: 6439 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5887 NdeI CA'TA_TG Cuts at: 387 2473 387 Size: 2086 4353 NgoMIV G'CCGG_C Cuts at: 2322 2322 Size: 6439 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6439 NotI GC'GGCC_GC Cuts at: 3886 3886 Size: 6439 NspI r_CATG'y Cuts at: 1132 1229 3797 5342 1132 Size: 97 2568 1545 2229 Fragments arranged by size: 2568 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6439 PciI A'CATG_T Cuts at: 5338 5338 Size: 6439 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6439 PfoI T'CCnGG_A Cuts at: 2207 2207 Size: 6439 PmeI GTTT'AAAC Cuts at: 3743 3743 Size: 6439 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2204 2236 2990 3022 4508 4540 6021 6053 2204 Size: 32 754 32 1486 32 1481 32 2590 Fragments arranged by size: 2590 1486 1481 754 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2708 1613 Size: 1095 5344 PsiI TTA'TAA Cuts at: 4009 4009 Size: 6439 PspOMI G'GGCC_C Cuts at: 3155 3534 3155 Size: 379 6060 PstI C_TGCA'G Cuts at: 830 2991 3086 3129 3599 3729 3791 830 Size: 2161 95 43 470 130 62 3478 Fragments arranged by size: 3478 2161 470 130 95 62 43 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6439 PvuII CAG'CTG Cuts at: 2776 3081 3320 3594 2776 Size: 305 239 274 5621 Fragments arranged by size: 5621 305 274 239 SalI G'TCGA_C Cuts at: 1936 3781 1936 Size: 1845 4594 SapI GCTCTTCn'nnn_ Cuts at: 2822 2991 2822 Size: 169 6270 SbfI CC_TGCA'GG Cuts at: 3129 3791 3129 Size: 662 5777 ScaI AGT'ACT Cuts at: 4687 6399 4687 Size: 1712 4727 SexAI A'CCwGG_T Cuts at: 3696 3696 Size: 6439 SfcI C'TryA_G Cuts at: 826 1045 2227 2987 3082 3125 3595 3725 3787 Size: 219 1182 760 95 43 470 130 62 Cuts at: 3787 4922 5603 5794 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1182 1135 760 681 470 219 191 130 95 62 43 SfoI GGC'GCC Cuts at: 2789 2789 Size: 6439 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6439 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6439 SmaI CCC'GGG Cuts at: 1872 3766 1872 Size: 1894 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3586 4543 5444 5706 820 Size: 197 926 1643 957 901 262 1553 Fragments arranged by size: 1643 1553 957 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4230 493 Size: 3737 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6439 SphI G_CATG'C Cuts at: 1132 3797 1132 Size: 2665 3774 SspI AAT'ATT Cuts at: 5 52 4363 5 Size: 47 4311 2081 Fragments arranged by size: 4311 2081 47 StuI AGG'CCT Cuts at: 1517 2903 1517 Size: 1386 5053 StyI C'CwwG_G Cuts at: 513 1065 2265 3267 3421 3483 3878 513 Size: 552 1200 1002 154 62 395 3074 Fragments arranged by size: 3074 1200 1002 552 395 154 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2158 4466 4483 4636 4821 5266 325 Size: 1255 578 2308 17 153 185 445 1498 Fragments arranged by size: 2308 1498 1255 578 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4685 6397 96 Size: 274 80 33 51 1443 2708 1712 138 Fragments arranged by size: 2708 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6399 6431 6399 Size: 32 6407 Tth111I GACn'n_nGTC Cuts at: 1316 2239 3698 1316 Size: 923 1459 4057 Fragments arranged by size: 4057 1459 923 XbaI T'CTAG_A Cuts at: 3508 3775 3508 Size: 267 6172 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2449 3203 3546 2449 Size: 754 343 5342 Fragments arranged by size: 5342 754 343 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6439 XmaI C'CCGG_G Cuts at: 1870 3764 1870 Size: 1894 4545 XmnI GAAnn'nnTTC Cuts at: 4568 4568 Size: 6439 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4247 276 Size: 53 83 186 936 2713 2468 Fragments arranged by size: 2713 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI