################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AgeI AhdI Alw44I AlwNI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvrII BalI BbeI BbrPI BciVI BclI BfaI BfrI BlnI BsaHI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I EarI EcoNI EcoO65I EcoRV EcoT22I FbaI HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I MaeI MfeI MluI MluNI MscI MseI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 1963 GTCGAC GT^[AC][GT]AC AcsI 937 GAATTC [GA]^AATT[CT] ApaI 1384 GGGCCC GGGCC^C ApoI 937 GAATTC [GA]^AATT[CT] AviII 648 TGCGCA TGC^GCA BanI 1208 GGCACC G^G[CT][GA]CC BbsI 1486 GAAGAC GAAGAC(2/6) BbvCI 1695 GCTGAGG CCTCAGC(-5/-2) BpuAI 1486 GAAGAC GAAGAC(2/6) BsaAI 1912 TACGTG [CT]AC^GT[GA] BsaBI 1773 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1410 GAGACC GGTCTC(1/5) BsgI 194 GTGCAG GTGCAG(16/14) BsmBI 1942 GAGACG CGTCTC(1/5) BsmI 559 GCATTC GAATGC(1/-1) BsrDI 1868 GCAATG GCAATG(2/0) BsrI 363 ACTGG ACTGG(1/-1) BstXI 1340 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EcoRI 937 GAATTC G^AATTC FseI 929 GGCCGGCC GGCCGG^CC FspI 648 TGCGCA TGC^GCA HincII 1963 GTCGAC GT[CT]^[GA]AC HindII 1963 GTCGAC GT[CT]^[GA]AC KspI 925 CCGCGG CCGC^GG MamI 1773 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 1767 CACGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG SacI 1170 GAGCTC GAGCT^C SacII 925 CCGCGG CCGC^GG SalI 1963 GTCGAC G^TCGAC SmaI 1380 CCCGGG CCC^GGG SmlI 1096 CTCAAG C^T[CT][GA]AG Tsp509I 938 AATT ^AATT XmaCI 1380 CCCGGG C^CCGGG XmaI 1380 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccIII 44 TCCGGA T^CCGGA 1727 TCCGGA AflIII 665 ACGTGT A^C[GA][CT]GT 1798 ACACGT Asp700I 132 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1731 GAAGAAGTTC AspHI 160 GAGCAC G[AT]GC[AT]^C 1170 GAGCTC BamHI 1644 GGATCC G^GATCC 1826 GGATCC BcgI 1398 GCAGAGCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1814 GCAAGCACCTCG BglII 1431 AGATCT A^GATCT 1511 AGATCT BlpI 153 GCTAAGC GC^T[ACGT]AGC 1215 GCTCAGC Bpu1102I 153 GCTAAGC GC^T[ACGT]AGC 1215 GCTCAGC BsaWI 44 TCCGGA [AT]^CCGG[AT] 1727 TCCGGA BseAI 44 TCCGGA T^CCGGA 1727 TCCGGA BsiHKAI 160 GAGCAC G[AT]GC[AT]^C 1170 GAGCTC BspEI 44 TCCGGA T^CCGGA 1727 TCCGGA CelII 153 GCTAAGC GC^T[ACGT]AGC 1215 GCTCAGC MroI 44 TCCGGA T^CCGGA 1727 TCCGGA NcoI 23 CCATGG C^CATGG 1670 CCATGG NlaIII 24 CATG CATG^ 1671 CATG PvuII 397 CAGCTG CAG^CTG 1693 CAGCTG XmnI 132 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1731 GAAGAAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AocI Aor51HI AvaI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII