(Circular) (Six-base) MAPSORT of: pFN21AE1490.seq Check: 8841 from: 1 to: 6715 pFN21AE1490 6715 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2025 2025 Size: 6715 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4525 278 Size: 53 83 186 936 2989 2468 Fragments arranged by size: 2989 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3969 4058 1937 Size: 2032 89 4594 Fragments arranged by size: 4594 2032 89 Acc65I G'GTAC_C Cuts at: 4036 4036 Size: 6715 AclI AA'CG_TT Cuts at: 1563 4842 5215 1563 Size: 3279 373 3063 Fragments arranged by size: 3279 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2156 3187 3510 3713 4778 6162 6430 1632 Size: 524 1031 323 203 1065 1384 268 1917 Fragments arranged by size: 1917 1384 1065 1031 524 323 268 203 AfeI AGC'GCT Cuts at: 2952 3094 3633 3933 2952 Size: 142 539 300 5734 Fragments arranged by size: 5734 539 300 142 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6518 AflIII A'CryG_T Cuts at: 1203 2670 3803 5614 6591 1203 Size: 1467 1133 1811 977 1327 Fragments arranged by size: 1811 1467 1327 1133 977 AgeI A'CCGG_T Cuts at: 5546 5546 Size: 6715 AhdI GACnn_n'nnGTC Cuts at: 5444 5444 Size: 6715 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6715 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6715 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3258 3290 3258 Size: 32 6683 AlwNI CAG_nnn'CTG Cuts at: 4458 6030 4458 Size: 1572 5143 ApaI G_GGCC'C Cuts at: 3393 3393 Size: 6715 ApaLI G'TGCA_C Cuts at: 4772 5928 4772 Size: 1156 5559 ApoI r'AATT_y Cuts at: 1493 2942 4024 4250 4402 6511 1493 Size: 1449 1082 226 152 2109 1697 Fragments arranged by size: 2109 1697 1449 1082 226 152 AseI AT'TA_AT Cuts at: 160 1032 5269 160 Size: 872 4237 1606 Fragments arranged by size: 4237 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6715 AvaI C'yCGr_G Cuts at: 1870 1943 2588 2608 2975 3011 3385 3827 4040 Size: 73 645 20 367 36 374 442 213 Cuts at: 4040 1870 Size: 4545 Fragments arranged by size: 4545 645 442 374 367 213 73 36 20 BamHI G'GATC_C Cuts at: 3649 3831 4045 4412 3649 Size: 182 214 367 5952 Fragments arranged by size: 5952 367 214 182 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3213 4036 5491 618 Size: 325 217 627 1426 823 1455 1842 Fragments arranged by size: 1842 1455 1426 823 627 325 217 BanII G_rGCy'C Cuts at: 1391 2091 2904 3012 3090 3179 3393 3468 4034 Size: 700 813 108 78 89 214 75 566 Cuts at: 4034 1391 Size: 4072 Fragments arranged by size: 4072 813 700 566 214 108 89 78 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3498 928 Size: 971 1599 4145 Fragments arranged by size: 4145 1599 971 BbvCI CC'TCA_GC Cuts at: 3701 3701 Size: 6715 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3392 3426 3808 3842 4906 4940 1903 Size: 34 1455 34 382 34 1064 34 3678 Fragments arranged by size: 3678 1455 1064 382 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4607 5817 4607 Size: 1210 5505 BclI T'GATC_A Cuts at: 1548 1548 Size: 6715 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2600 3388 3573 Size: 107 122 71 631 605 928 788 185 Cuts at: 3573 5326 136 Size: 1753 1525 Fragments arranged by size: 1753 1525 928 788 631 605 185 122 107 71 BglII A'GATC_T Cuts at: 3436 3516 6710 3436 Size: 80 3194 3441 Fragments arranged by size: 3441 3194 80 BlpI GC'TnA_GC Cuts at: 2159 3221 4132 2159 Size: 1062 911 4742 Fragments arranged by size: 4742 1062 911 Bme1580I G_kGCm'C Cuts at: 1790 3393 3924 4776 5932 1790 Size: 1603 531 852 1156 2573 Fragments arranged by size: 2573 1603 1156 852 531 BmgBI CAC'GTC Cuts at: 1318 3806 1318 Size: 2488 4227 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4446 5404 442 Size: 416 53 83 399 24 3029 958 1753 Fragments arranged by size: 3029 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6715 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3037 3037 Size: 6715 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2390 2785 3277 3348 3541 3872 3965 5375 Size: 1151 395 492 71 193 331 93 1410 Cuts at: 5375 1239 Size: 2579 Fragments arranged by size: 2579 1410 1151 492 395 331 193 93 71 Bpu10I CC'TnA_GC Cuts at: 2154 2868 3084 3635 3701 2154 Size: 714 216 551 66 5168 Fragments arranged by size: 5168 714 551 216 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3086 4840 5705 6003 3086 Size: 1754 865 298 3798 Fragments arranged by size: 3798 1754 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3409 5378 882 Size: 617 1910 1969 2219 Fragments arranged by size: 2219 1969 1910 617 BsaAI yAC'GTr Cuts at: 493 1204 3919 4506 493 Size: 711 2715 587 2702 Fragments arranged by size: 2715 2702 711 587 BsaBI GATnn'nnATC Cuts at: 3782 6709 3782 Size: 2927 3788 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4522 4904 5539 275 Size: 53 83 186 936 2989 382 635 1451 Fragments arranged by size: 2989 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2049 3732 5148 5546 5820 5967 6378 2049 Size: 1683 1416 398 274 147 411 2386 Fragments arranged by size: 2386 1683 1416 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3258 3288 3872 3902 1178 Size: 30 165 30 1855 30 584 30 3991 Fragments arranged by size: 3991 1855 584 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2884 2992 3893 1186 Size: 1698 108 901 4008 Fragments arranged by size: 4008 1698 901 108 BseYI C'CCAG_C Cuts at: 2090 2148 2404 2826 2980 3089 3158 3242 3308 Size: 58 256 422 154 109 69 84 66 Cuts at: 3308 3461 3563 3628 3654 5918 6414 2090 Size: 153 102 65 26 2264 496 2391 Fragments arranged by size: 2391 2264 496 422 256 154 153 109 102 84 69 66 65 58 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2220 1163 Size: 573 484 5658 Fragments arranged by size: 5658 573 484 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2936 2940 3192 3459 4165 4926 Size: 275 186 935 4 252 267 706 761 Cuts at: 4926 5954 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 935 761 706 275 267 252 186 4 BsiHKAI G_wGCw'C Cuts at: 2169 3179 4776 4861 5932 2169 Size: 1010 1597 85 1071 2952 Fragments arranged by size: 2952 1597 1071 1010 85 BsmI GAATG_Cn' Cuts at: 2564 4226 4319 2564 Size: 1662 93 4960 Fragments arranged by size: 4960 1662 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3941 3941 Size: 6715 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2169 2904 3012 3090 3179 3393 Size: 399 301 78 735 108 78 89 214 Cuts at: 3393 3468 3924 4034 4776 4861 5932 1391 Size: 75 456 110 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 735 456 399 301 214 110 108 89 85 78 78 75 BspEI T'CCGG_A Cuts at: 2049 3732 6378 2049 Size: 1683 2646 2386 Fragments arranged by size: 2646 2386 1683 BspHI T'CATG_A Cuts at: 4604 6334 4604 Size: 1730 4985 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2025 2106 3493 4070 844 Size: 1181 81 1387 577 3489 Fragments arranged by size: 3489 1387 1181 577 81 BsrBI CCG'CTC Cuts at: 1394 1823 2753 3220 3453 4602 1394 Size: 429 930 467 233 1149 3507 Fragments arranged by size: 3507 1149 930 467 429 233 BsrDI GCAATG_nn' Cuts at: 65 1248 3880 5210 5384 65 Size: 1183 2632 1330 174 1396 Fragments arranged by size: 2632 1396 1330 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2491 2594 2935 3197 5359 5546 1700 Size: 110 681 103 341 262 2162 187 2869 Fragments arranged by size: 2869 2162 681 341 262 187 110 103 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6715 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6715 BssSI C'ACGA_G Cuts at: 4775 5787 4775 Size: 1012 5703 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6715 BstBI TT'CG_AA Cuts at: 5519 5519 Size: 6715 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6715 BstXI CCAn_nnnn'nTGG Cuts at: 3352 3352 Size: 6715 BstYI r'GATC_y Cuts at: 1972 3436 3516 3649 3831 3846 4045 4412 4797 Size: 1464 80 133 182 15 199 367 385 Cuts at: 4797 4814 6266 6352 6364 6710 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1464 1452 385 367 346 199 182 133 86 80 17 15 12 Bsu36I CC'TnA_GG Cuts at: 2226 2262 2298 2334 2442 2697 2226 Size: 36 36 36 108 255 6244 Fragments arranged by size: 6244 255 108 36 36 36 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 2930 3192 3393 3675 513 Size: 552 939 24 902 262 201 282 3553 Fragments arranged by size: 3553 939 902 552 282 262 201 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5976 Fragments arranged by size: 5976 683 56 BtsI GCAGTG_nn' Cuts at: 2208 3179 4231 5025 5045 2208 Size: 971 1052 794 20 3878 Fragments arranged by size: 3878 1052 971 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6715 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3648 3683 529 Size: 35 3084 35 3561 Fragments arranged by size: 3561 3084 35 35 DraI TTT'AAA Cuts at: 4019 4019 Size: 6715 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5722 809 Size: 507 4406 1802 Fragments arranged by size: 4406 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2933 2937 3189 Size: 54 1425 50 275 18 1103 4 252 Cuts at: 3189 3195 3456 4162 5051 6680 8 Size: 6 261 706 889 1629 43 Fragments arranged by size: 1629 1425 1103 889 706 275 261 252 54 50 43 18 6 4 EagI C'GGCC_G Cuts at: 1537 1812 2933 2937 3189 3456 4162 1537 Size: 275 1121 4 252 267 706 4090 Fragments arranged by size: 4090 1121 706 275 267 252 4 EarI CTCTTCn'nnn_ Cuts at: 1353 4645 1353 Size: 3292 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3340 5302 5676 5822 387 Size: 1064 138 1751 1962 374 146 1280 Fragments arranged by size: 1962 1751 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3177 3177 Size: 6715 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2390 2785 3187 3277 3348 3510 Size: 393 524 234 395 402 90 71 162 Cuts at: 3510 3541 3713 3872 3965 4778 5375 6162 6430 Size: 31 172 159 93 813 597 787 268 Cuts at: 6430 1239 Size: 1524 Fragments arranged by size: 1524 813 787 597 524 402 395 393 268 234 172 162 159 93 90 71 31 EcoO109I rG'GnC_Cy Cuts at: 2179 2455 2613 2878 2889 2997 3389 3447 3505 Size: 276 158 265 11 108 392 58 58 Cuts at: 3505 3895 2179 Size: 390 4999 Fragments arranged by size: 4999 392 390 276 265 158 108 58 58 11 EcoRI G'AATT_C Cuts at: 2942 4024 4402 2942 Size: 1082 378 5255 Fragments arranged by size: 5255 1082 378 FseI GG_CCGG'CC Cuts at: 2939 2939 Size: 6715 FspI TGC'GCA Cuts at: 2655 5221 2655 Size: 2566 4149 HaeII r_GCGC'y Cuts at: 1678 2808 2954 3055 3096 3590 3635 3935 5862 Size: 1130 146 101 41 494 45 300 1927 Cuts at: 5862 1678 Size: 2531 Fragments arranged by size: 2531 1927 1130 494 300 146 101 45 41 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5356 5388 5430 5462 1373 Size: 32 135 32 3784 32 42 32 2626 Fragments arranged by size: 3784 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3970 4059 4305 669 Size: 988 281 2032 89 246 3079 Fragments arranged by size: 3079 2032 988 281 246 89 HindIII A'AGCT_T Cuts at: 748 748 Size: 6715 HpaI GTT'AAC Cuts at: 4305 4305 Size: 6715 KpnI G_GTAC'C Cuts at: 4040 4040 Size: 6715 MfeI C'AATT_G Cuts at: 4314 4314 Size: 6715 MluI A'CGCG_T Cuts at: 6591 6591 Size: 6715 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5829 6013 1305 Size: 4524 184 2007 Fragments arranged by size: 4524 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4893 Fragments arranged by size: 4893 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3776 4673 5032 5191 518 Size: 648 2610 897 359 159 2042 Fragments arranged by size: 2610 2042 897 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2185 2404 2836 2932 3242 3255 3379 3461 Size: 579 219 432 96 310 13 124 82 Cuts at: 3461 3700 4131 4808 5956 6201 1606 Size: 239 431 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 579 432 431 310 245 239 219 124 96 82 13 NaeI GCC'GGC Cuts at: 2493 2937 3199 2493 Size: 444 262 6009 Fragments arranged by size: 6009 444 262 NcoI C'CATG_G Cuts at: 513 1065 2004 2028 3675 513 Size: 552 939 24 1647 3553 Fragments arranged by size: 3553 1647 939 552 24 NdeI CA'TA_TG Cuts at: 387 387 Size: 6715 NgoMIV G'CCGG_C Cuts at: 2491 2935 3197 2491 Size: 444 262 6009 Fragments arranged by size: 6009 444 262 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6715 NotI GC'GGCC_GC Cuts at: 4162 4162 Size: 6715 NspI r_CATG'y Cuts at: 1132 1229 4073 5618 1132 Size: 97 2844 1545 2229 Fragments arranged by size: 2844 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3227 4006 1348 Size: 1879 779 4057 Fragments arranged by size: 4057 1879 779 PciI A'CATG_T Cuts at: 5614 5614 Size: 6715 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6715 PfoI T'CCnGG_A Cuts at: 3474 3849 3474 Size: 375 6340 PmeI GTTT'AAAC Cuts at: 4019 4019 Size: 6715 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2999 3031 3258 3290 3713 3745 4784 4816 6297 Size: 32 227 32 423 32 1039 32 1481 Cuts at: 6297 6329 2999 Size: 32 3385 Fragments arranged by size: 3385 1481 1039 423 227 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2613 2878 2889 2997 3447 3505 3895 2613 Size: 265 11 108 450 58 390 5433 Fragments arranged by size: 5433 450 390 265 108 58 11 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6715 PsiI TTA'TAA Cuts at: 4285 4285 Size: 6715 PspOMI G'GGCC_C Cuts at: 3389 3389 Size: 6715 PstI C_TGCA'G Cuts at: 830 2026 2439 2511 3045 3427 4067 830 Size: 1196 413 72 534 382 640 3478 Fragments arranged by size: 3478 1196 640 534 413 382 72 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6715 PvuII CAG'CTG Cuts at: 2404 3700 2404 Size: 1296 5419 SacI G_AGCT'C Cuts at: 3179 3179 Size: 6715 SacII CC_GC'GG Cuts at: 2933 2933 Size: 6715 SalI G'TCGA_C Cuts at: 1936 3968 4057 1936 Size: 2032 89 4594 Fragments arranged by size: 4594 2032 89 SanDI GG'GwC_CC Cuts at: 3895 3895 Size: 6715 SbfI CC_TGCA'GG Cuts at: 4067 4067 Size: 6715 ScaI AGT'ACT Cuts at: 4963 6675 4963 Size: 1712 5003 SfcI C'TryA_G Cuts at: 826 1045 2022 2435 2507 3041 3423 4063 5198 Size: 219 977 413 72 534 382 640 1135 Cuts at: 5198 5879 6070 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 977 681 640 534 413 382 219 191 72 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6715 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6715 SmaI CCC'GGG Cuts at: 1872 3387 4042 1872 Size: 1515 655 4545 Fragments arranged by size: 4545 1515 655 SmlI C'TyrA_G Cuts at: 820 1017 1943 3101 4819 5720 5982 820 Size: 197 926 1158 1718 901 262 1553 Fragments arranged by size: 1718 1553 1158 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4506 493 Size: 4013 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6715 SphI G_CATG'C Cuts at: 1132 4073 1132 Size: 2941 3774 SspI AAT'ATT Cuts at: 5 52 4639 5 Size: 47 4587 2081 Fragments arranged by size: 4587 2081 47 StuI AGG'CCT Cuts at: 1517 2075 2468 2822 3270 3300 3331 1517 Size: 558 393 354 448 30 31 4901 Fragments arranged by size: 4901 558 448 393 354 31 30 StyI C'CwwG_G Cuts at: 513 1065 2004 2028 2423 3570 3675 4154 513 Size: 552 939 24 395 1147 105 479 3074 Fragments arranged by size: 3074 1147 939 552 479 395 105 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3495 3688 4742 4759 4912 5097 5542 Size: 1255 1915 193 1054 17 153 185 445 Cuts at: 5542 325 Size: 1498 Fragments arranged by size: 1915 1498 1255 1054 445 193 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4961 6673 96 Size: 274 80 33 51 1443 2984 1712 138 Fragments arranged by size: 2984 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2404 2436 3872 3904 6675 6707 2404 Size: 32 1436 32 2771 32 2412 Fragments arranged by size: 2771 2412 1436 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6715 XbaI T'CTAG_A Cuts at: 4051 4051 Size: 6715 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6715 XmaI C'CCGG_G Cuts at: 1870 3385 4040 1870 Size: 1515 655 4545 Fragments arranged by size: 4545 1515 655 XmnI GAAnn'nnTTC Cuts at: 2141 3740 4844 2141 Size: 1599 1104 4012 Fragments arranged by size: 4012 1599 1104 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4523 276 Size: 53 83 186 936 2989 2468 Fragments arranged by size: 2989 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI XcmI