(Linear) (Six-base) MAPSORT of: KIEE1490.seq Check: 6798 from: 1 to: 2010 KIEE1490.orf With 182 enzymes: SgfI * July 15, 2010 18:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 20 2010 Size: 20 1990 AccI GT'mk_AC Cuts at: 0 1964 2010 Size: 1964 46 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 1182 1505 1708 2010 Size: 151 1031 323 203 302 Fragments arranged by size: 1031 323 302 203 151 AfeI AGC'GCT Cuts at: 0 947 1089 1628 1928 2010 Size: 947 142 539 300 82 Fragments arranged by size: 947 539 300 142 82 AflIII A'CryG_T Cuts at: 0 665 1798 2010 Size: 665 1133 212 Fragments arranged by size: 1133 665 212 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1253 1285 2010 Size: 1253 32 725 Fragments arranged by size: 1253 725 32 ApaI G_GGCC'C Cuts at: 0 1388 2010 Size: 1388 622 ApoI r'AATT_y Cuts at: 0 937 2010 Size: 937 1073 AvaI C'yCGr_G Cuts at: 0 583 603 970 1006 1380 1822 2010 Size: 583 20 367 36 374 442 188 Fragments arranged by size: 583 442 374 367 188 36 20 BamHI G'GATC_C Cuts at: 0 1644 1826 2010 Size: 1644 182 184 Fragments arranged by size: 1644 184 182 BanI G'GyrC_C Cuts at: 0 1208 2010 Size: 1208 802 BanII G_rGCy'C Cuts at: 0 86 899 1007 1085 1174 1388 1463 2010 Size: 86 813 108 78 89 214 75 547 Fragments arranged by size: 813 547 214 108 89 86 78 75 BbsI GAAGACnn'nnnn_ Cuts at: 0 1493 2010 Size: 1493 517 BbvCI CC'TCA_GC Cuts at: 0 1696 2010 Size: 1696 314 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1387 1421 1803 1837 2010 Size: 1387 34 382 34 173 Fragments arranged by size: 1387 382 173 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 595 1383 1568 2010 Size: 595 788 185 442 Fragments arranged by size: 788 595 442 185 BglII A'GATC_T Cuts at: 0 1431 1511 2010 Size: 1431 80 499 Fragments arranged by size: 1431 499 80 BlpI GC'TnA_GC Cuts at: 0 154 1216 2010 Size: 154 1062 794 Fragments arranged by size: 1062 794 154 Bme1580I G_kGCm'C Cuts at: 0 1388 1919 2010 Size: 1388 531 91 Fragments arranged by size: 1388 531 91 BmgBI CAC'GTC Cuts at: 0 1801 2010 Size: 1801 209 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1032 2010 Size: 1032 978 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 385 780 1272 1343 1536 1867 1960 2010 Size: 385 395 492 71 193 331 93 50 Fragments arranged by size: 492 395 385 331 193 93 71 50 Bpu10I CC'TnA_GC Cuts at: 0 149 863 1079 1630 1696 2010 Size: 149 714 216 551 66 314 Fragments arranged by size: 714 551 314 216 149 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1081 2010 Size: 1081 929 BsaI GGTCTCn'nnnn_ Cuts at: 0 1404 2010 Size: 1404 606 BsaAI yAC'GTr Cuts at: 0 1914 2010 Size: 1914 96 BsaBI GATnn'nnATC Cuts at: 0 1777 2010 Size: 1777 233 BsaWI w'CCGG_w Cuts at: 0 44 1727 2010 Size: 44 1683 283 Fragments arranged by size: 1683 283 44 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1253 1283 1867 1897 2010 Size: 1253 30 584 30 113 Fragments arranged by size: 1253 584 113 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 879 987 1888 2010 Size: 879 108 901 122 Fragments arranged by size: 901 879 122 108 BseYI C'CCAG_C Cuts at: 0 85 143 399 821 975 1084 1153 1237 Size: 85 58 256 422 154 109 69 84 Cuts at: 1237 1303 1456 1558 1623 1649 2010 Size: 66 153 102 65 26 361 Fragments arranged by size: 422 361 256 154 153 109 102 85 84 69 66 65 58 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 215 2010 Size: 215 1795 BsiEI CG_ry'CG Cuts at: 0 931 935 1187 1454 2010 Size: 931 4 252 267 556 Fragments arranged by size: 931 556 267 252 4 BsiHKAI G_wGCw'C Cuts at: 0 164 1174 2010 Size: 164 1010 836 Fragments arranged by size: 1010 836 164 BsmI GAATG_Cn' Cuts at: 0 559 2010 Size: 559 1451 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1936 2010 Size: 1936 74 Bsp1286I G_dGCh'C Cuts at: 0 86 164 899 1007 1085 1174 1388 1463 Size: 86 78 735 108 78 89 214 75 Cuts at: 1463 1919 2010 Size: 456 91 Fragments arranged by size: 735 456 214 108 91 89 86 78 78 75 BspEI T'CCGG_A Cuts at: 0 44 1727 2010 Size: 44 1683 283 Fragments arranged by size: 1683 283 44 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 20 101 1488 2010 Size: 20 81 1387 522 Fragments arranged by size: 1387 522 81 20 BsrBI CCG'CTC Cuts at: 0 748 1215 1448 2010 Size: 748 467 233 562 Fragments arranged by size: 748 562 467 233 BsrDI GCAATG_nn' Cuts at: 0 1875 2010 Size: 1875 135 BsrFI r'CCGG_y Cuts at: 0 486 589 930 1192 2010 Size: 486 103 341 262 818 Fragments arranged by size: 818 486 341 262 103 BstXI CCAn_nnnn'nTGG Cuts at: 0 1347 2010 Size: 1347 663 BstYI r'GATC_y Cuts at: 0 1431 1511 1644 1826 1841 2010 Size: 1431 80 133 182 15 169 Fragments arranged by size: 1431 182 169 133 80 15 Bsu36I CC'TnA_GG Cuts at: 0 221 257 293 329 437 692 2010 Size: 221 36 36 36 108 255 1318 Fragments arranged by size: 1318 255 221 108 36 36 36 BtgI C'CryG_G Cuts at: 0 23 925 1187 1388 1670 2010 Size: 23 902 262 201 282 340 Fragments arranged by size: 902 340 282 262 201 23 BtsI GCAGTG_nn' Cuts at: 0 203 1174 2010 Size: 203 971 836 Fragments arranged by size: 971 836 203 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1643 1678 2010 Size: 1643 35 332 Fragments arranged by size: 1643 332 35 EaeI y'GGCC_r Cuts at: 0 928 932 1184 1190 1451 2010 Size: 928 4 252 6 261 559 Fragments arranged by size: 928 559 261 252 6 4 EagI C'GGCC_G Cuts at: 0 928 932 1184 1451 2010 Size: 928 4 252 267 559 Fragments arranged by size: 928 559 267 252 4 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1335 2010 Size: 1335 675 EcoICRI GAG'CTC Cuts at: 0 1172 2010 Size: 1172 838 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 385 780 1182 1272 1343 1505 1536 Size: 151 234 395 402 90 71 162 31 Cuts at: 1536 1708 1867 1960 2010 Size: 172 159 93 50 Fragments arranged by size: 402 395 234 172 162 159 151 93 90 71 50 31 EcoO109I rG'GnC_Cy Cuts at: 0 174 450 608 873 884 992 1384 1442 Size: 174 276 158 265 11 108 392 58 Cuts at: 1442 1500 1890 2010 Size: 58 390 120 Fragments arranged by size: 392 390 276 265 174 158 120 108 58 58 11 EcoRI G'AATT_C Cuts at: 0 937 2010 Size: 937 1073 FseI GG_CCGG'CC Cuts at: 0 934 2010 Size: 934 1076 FspI TGC'GCA Cuts at: 0 650 2010 Size: 650 1360 HaeII r_GCGC'y Cuts at: 0 803 949 1050 1091 1585 1630 1930 2010 Size: 803 146 101 41 494 45 300 80 Fragments arranged by size: 803 494 300 146 101 80 45 41 HincII GTy'rAC Cuts at: 0 1965 2010 Size: 1965 45 MslI CAynn'nnrTG Cuts at: 0 1771 2010 Size: 1771 239 MspA1I CmG'CkG Cuts at: 0 180 399 831 927 1237 1250 1374 1456 Size: 180 219 432 96 310 13 124 82 Cuts at: 1456 1695 2010 Size: 239 315 Fragments arranged by size: 432 315 310 239 219 180 124 96 82 13 NaeI GCC'GGC Cuts at: 0 488 932 1194 2010 Size: 488 444 262 816 Fragments arranged by size: 816 488 444 262 NcoI C'CATG_G Cuts at: 0 23 1670 2010 Size: 23 1647 340 Fragments arranged by size: 1647 340 23 NgoMIV G'CCGG_C Cuts at: 0 486 930 1192 2010 Size: 486 444 262 818 Fragments arranged by size: 818 486 444 262 PasI CC'CwG_GG Cuts at: 0 1222 2001 2010 Size: 1222 779 9 Fragments arranged by size: 1222 779 9 PfoI T'CCnGG_A Cuts at: 0 1469 1844 2010 Size: 1469 375 166 Fragments arranged by size: 1469 375 166 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 994 1026 1253 1285 1708 1740 2010 Size: 994 32 227 32 423 32 270 Fragments arranged by size: 994 423 270 227 32 32 32 PpuMI rG'GwC_Cy Cuts at: 0 608 873 884 992 1442 1500 1890 2010 Size: 608 265 11 108 450 58 390 120 Fragments arranged by size: 608 450 390 265 120 108 58 11 PspOMI G'GGCC_C Cuts at: 0 1384 2010 Size: 1384 626 PstI C_TGCA'G Cuts at: 0 21 434 506 1040 1422 2010 Size: 21 413 72 534 382 588 Fragments arranged by size: 588 534 413 382 72 21 PvuII CAG'CTG Cuts at: 0 399 1695 2010 Size: 399 1296 315 Fragments arranged by size: 1296 399 315 SacI G_AGCT'C Cuts at: 0 1174 2010 Size: 1174 836 SacII CC_GC'GG Cuts at: 0 928 2010 Size: 928 1082 SalI G'TCGA_C Cuts at: 0 1963 2010 Size: 1963 47 SanDI GG'GwC_CC Cuts at: 0 1890 2010 Size: 1890 120 SfcI C'TryA_G Cuts at: 0 17 430 502 1036 1418 2010 Size: 17 413 72 534 382 592 Fragments arranged by size: 592 534 413 382 72 17 SmaI CCC'GGG Cuts at: 0 1382 2010 Size: 1382 628 SmlI C'TyrA_G Cuts at: 0 1096 2010 Size: 1096 914 StuI AGG'CCT Cuts at: 0 70 463 817 1265 1295 1326 2010 Size: 70 393 354 448 30 31 684 Fragments arranged by size: 684 448 393 354 70 31 30 StyI C'CwwG_G Cuts at: 0 23 418 1565 1670 2010 Size: 23 395 1147 105 340 Fragments arranged by size: 1147 395 340 105 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1490 1683 2010 Size: 1490 193 327 Fragments arranged by size: 1490 327 193 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 399 431 1867 1899 2010 Size: 399 32 1436 32 111 Fragments arranged by size: 1436 399 111 32 32 XmaI C'CCGG_G Cuts at: 0 1380 2010 Size: 1380 630 XmnI GAAnn'nnTTC Cuts at: 0 136 1735 2010 Size: 136 1599 275 Fragments arranged by size: 1599 275 136 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AloI ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI EagI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII HincII MslI MspA1I NaeI NcoI NgoMIV PasI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SacII SalI SanDI SfcI SmaI SmlI StuI StyI TaqII TstI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AhdI AleI AlfI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BmrI BmtI BsaHI BsiWI BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EarI EcoNI EcoRV FalI FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI XhoI ZraI