(Circular) (Six-base) MAPSORT of: pFN21AE0955.seq Check: 9396 from: 1 to: 6154 pFN21AE0955 6154 bp With 182 enzymes: SgfI * March 28, 2012 12:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3964 278 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3419 3497 1937 Size: 1482 78 4594 Fragments arranged by size: 4594 1482 78 Acc65I G'GTAC_C Cuts at: 3475 3475 Size: 6154 AclI AA'CG_TT Cuts at: 1563 4281 4654 1563 Size: 2718 373 3063 Fragments arranged by size: 3063 2718 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3262 4217 5601 5869 1632 Size: 1630 955 1384 268 1917 Fragments arranged by size: 1917 1630 1384 955 268 AfeI AGC'GCT Cuts at: 2342 2342 Size: 6154 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5957 AflIII A'CryG_T Cuts at: 1203 2676 5053 6030 1203 Size: 1473 2377 977 1327 Fragments arranged by size: 2377 1473 1327 977 AgeI A'CCGG_T Cuts at: 4985 4985 Size: 6154 AhdI GACnn_n'nnGTC Cuts at: 3415 4883 3415 Size: 1468 4686 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6154 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6154 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2453 2485 3053 3085 3282 3314 2453 Size: 32 568 32 197 32 5293 Fragments arranged by size: 5293 568 197 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2752 3897 5469 2752 Size: 1145 1572 3437 Fragments arranged by size: 3437 1572 1145 ApaI G_GGCC'C Cuts at: 2866 2866 Size: 6154 ApaLI G'TGCA_C Cuts at: 4211 5367 4211 Size: 1156 4998 ApoI r'AATT_y Cuts at: 1493 3463 3689 3841 5950 1493 Size: 1970 226 152 2109 1697 Fragments arranged by size: 2109 1970 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4708 160 Size: 872 3676 1606 Fragments arranged by size: 3676 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 AvaI C'yCGr_G Cuts at: 1870 1943 2044 2191 2270 2600 3024 3479 1870 Size: 73 101 147 79 330 424 455 4545 Fragments arranged by size: 4545 455 424 330 147 101 79 73 BamHI G'GATC_C Cuts at: 3484 3851 3484 Size: 367 5787 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2138 3475 4930 618 Size: 325 217 627 351 1337 1455 1842 Fragments arranged by size: 1842 1455 1337 627 351 325 217 BanII G_rGCy'C Cuts at: 1391 2045 2079 2198 2829 2866 2874 2943 3473 Size: 654 34 119 631 37 8 69 530 Cuts at: 3473 1391 Size: 4072 Fragments arranged by size: 4072 654 631 530 119 69 37 34 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5183 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4345 4379 1903 Size: 34 2408 34 3678 Fragments arranged by size: 3678 2408 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4046 5256 4046 Size: 1210 4944 BclI T'GATC_A Cuts at: 1548 1548 Size: 6154 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2040 2115 2136 Size: 107 122 71 631 605 368 75 21 Cuts at: 2136 4765 136 Size: 2629 1525 Fragments arranged by size: 2629 1525 631 605 368 122 107 75 71 21 BglII A'GATC_T Cuts at: 6149 6149 Size: 6154 BlpI GC'TnA_GC Cuts at: 3571 3571 Size: 6154 Bme1580I G_kGCm'C Cuts at: 1790 2601 2866 3356 4215 5371 1790 Size: 811 265 490 859 1156 2573 Fragments arranged by size: 2573 1156 859 811 490 265 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6154 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3885 4843 442 Size: 416 53 83 399 24 2468 958 1753 Fragments arranged by size: 2468 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6154 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2441 2530 3176 4814 1239 Size: 1202 89 646 1638 2579 Fragments arranged by size: 2579 1638 1202 646 89 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2765 3157 3358 4279 5144 5442 2765 Size: 392 201 921 865 298 3477 Fragments arranged by size: 3477 921 865 392 298 201 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2417 4817 882 Size: 617 918 2400 2219 Fragments arranged by size: 2400 2219 918 617 BsaAI yAC'GTr Cuts at: 493 1204 3945 493 Size: 711 2741 2702 Fragments arranged by size: 2741 2702 711 BsaBI GATnn'nnATC Cuts at: 6148 6148 Size: 6154 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2181 3961 4343 4978 Size: 53 83 186 936 648 1780 382 635 Cuts at: 4978 275 Size: 1451 Fragments arranged by size: 1780 1451 936 648 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4587 4985 5259 5406 5817 4587 Size: 398 274 147 411 4924 Fragments arranged by size: 4924 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2696 2726 3053 3083 3379 Size: 30 165 30 1293 30 327 30 296 Cuts at: 3379 3409 1178 Size: 30 3923 Fragments arranged by size: 3923 1293 327 296 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2109 2241 2933 3321 3439 1186 Size: 923 132 692 388 118 3901 Fragments arranged by size: 3901 923 692 388 132 118 BseYI C'CCAG_C Cuts at: 2281 3010 3237 3346 5357 5853 2281 Size: 729 227 109 2011 496 2582 Fragments arranged by size: 2582 2011 729 496 227 109 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5581 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3423 3604 4365 5393 1540 Size: 275 186 1422 181 761 1028 2301 Fragments arranged by size: 2301 1422 1028 761 275 186 181 BsiHKAI G_wGCw'C Cuts at: 2198 2874 2943 4215 4300 5371 2198 Size: 676 69 1272 85 1071 2981 Fragments arranged by size: 2981 1272 1071 676 85 69 BsmI GAATG_Cn' Cuts at: 3665 3758 3665 Size: 93 6061 BsmBI CGTCTCn'nnnn_ Cuts at: 3312 3312 Size: 6154 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2079 2198 2601 2829 2866 2874 Size: 399 255 34 119 403 228 37 8 Cuts at: 2874 2943 3356 3473 4215 4300 5371 1391 Size: 69 413 117 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 413 403 399 255 228 119 117 85 69 37 34 8 BspEI T'CCGG_A Cuts at: 5817 5817 Size: 6154 BspHI T'CATG_A Cuts at: 4043 5773 4043 Size: 1730 4424 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2760 3509 844 Size: 1916 749 3489 Fragments arranged by size: 3489 1916 749 BsrBI CCG'CTC Cuts at: 1394 1823 2010 4041 1394 Size: 429 187 2031 3507 Fragments arranged by size: 3507 2031 429 187 BsrDI GCAATG_nn' Cuts at: 65 1248 2389 4649 4823 65 Size: 1183 1141 2260 174 1396 Fragments arranged by size: 2260 1396 1183 1141 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2141 4798 4985 1700 Size: 110 331 2657 187 2869 Fragments arranged by size: 2869 2657 331 187 110 BsrGI T'GTAC_A Cuts at: 96 2673 96 Size: 2577 3577 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6154 BssSI C'ACGA_G Cuts at: 4214 5226 4214 Size: 1012 5142 BstAPI GCAn_nnn'nTGC Cuts at: 794 2494 794 Size: 1700 4454 BstBI TT'CG_AA Cuts at: 4958 4958 Size: 6154 BstEII G'GTnAC_C Cuts at: 1184 2302 1184 Size: 1118 5036 BstYI r'GATC_y Cuts at: 1972 3484 3851 4236 4253 5705 5791 5803 6149 Size: 1512 367 385 17 1452 86 12 346 Cuts at: 6149 1972 Size: 1977 Fragments arranged by size: 1977 1512 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2169 513 Size: 552 1104 4498 Fragments arranged by size: 4498 1104 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3118 3238 487 Size: 683 56 1892 120 3403 Fragments arranged by size: 3403 1892 683 120 56 BtsI GCAGTG_nn' Cuts at: 2509 2769 2806 3670 4464 4484 2509 Size: 260 37 864 794 20 4179 Fragments arranged by size: 4179 864 794 260 37 20 ClaI AT'CG_AT Cuts at: 1554 2907 1554 Size: 1353 4801 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6119 DraI TTT'AAA Cuts at: 3458 3458 Size: 6154 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2774 5161 809 Size: 507 1458 2387 1802 Fragments arranged by size: 2387 1802 1458 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3601 4490 6119 Size: 54 1425 50 275 18 1771 889 1629 Cuts at: 6119 8 Size: 43 Fragments arranged by size: 1771 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3601 1537 Size: 275 1789 4090 Fragments arranged by size: 4090 1789 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2693 3275 4084 1353 Size: 1340 582 809 3423 Fragments arranged by size: 3423 1340 809 582 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2100 2106 2163 2238 4741 5115 Size: 1064 138 511 6 57 75 2503 374 Cuts at: 5115 5261 387 Size: 146 1280 Fragments arranged by size: 2503 1280 1064 511 374 146 138 75 57 6 EcoICRI GAG'CTC Cuts at: 2196 2872 2941 2196 Size: 676 69 5409 Fragments arranged by size: 5409 676 69 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2441 2530 3176 3262 4217 4814 5601 Size: 393 809 89 646 86 955 597 787 Cuts at: 5601 5869 1239 Size: 268 1524 Fragments arranged by size: 1524 955 809 787 646 597 393 268 89 86 EcoNI CCTnn'n_nnAGG Cuts at: 2025 2025 Size: 6154 EcoO109I rG'GnC_Cy Cuts at: 2187 2240 2863 3342 2187 Size: 53 623 479 4999 Fragments arranged by size: 4999 623 479 53 EcoRI G'AATT_C Cuts at: 3463 3841 3463 Size: 378 5776 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2585 2585 Size: 6154 FspI TGC'GCA Cuts at: 3201 4660 3201 Size: 1459 4695 FspAI rTGC'GCAy Cuts at: 3201 3201 Size: 6154 HaeII r_GCGC'y Cuts at: 1678 2344 5301 1678 Size: 666 2957 2531 Fragments arranged by size: 2957 2531 666 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2882 2900 2914 2932 3070 Size: 32 135 32 1310 18 14 18 138 Cuts at: 3070 3102 3401 3433 4795 4827 4869 4901 1373 Size: 32 299 32 1362 32 42 32 2626 Fragments arranged by size: 2626 1362 1310 299 138 135 42 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 2646 3420 3498 3744 669 Size: 988 281 708 774 78 246 3079 Fragments arranged by size: 3079 988 774 708 281 246 78 HindIII A'AGCT_T Cuts at: 748 3381 748 Size: 2633 3521 HpaI GTT'AAC Cuts at: 3744 3744 Size: 6154 KpnI G_GTAC'C Cuts at: 3479 3479 Size: 6154 MfeI C'AATT_G Cuts at: 3753 3753 Size: 6154 MluI A'CGCG_T Cuts at: 6030 6030 Size: 6154 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3325 3422 5268 5452 1305 Size: 2020 97 1846 184 2007 Fragments arranged by size: 2020 2007 1846 184 97 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4332 Fragments arranged by size: 4332 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2372 3005 4112 4471 4630 518 Size: 648 1206 633 1107 359 159 2042 Fragments arranged by size: 2042 1206 1107 648 633 359 159 MspA1I CmG'CkG Cuts at: 1606 2220 2235 2295 2921 2989 3241 3350 3570 Size: 614 15 60 626 68 252 109 220 Cuts at: 3570 4247 5395 5640 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 626 614 252 245 220 109 68 60 15 NcoI C'CATG_G Cuts at: 513 1065 2169 513 Size: 552 1104 4498 Fragments arranged by size: 4498 1104 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6154 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6154 NotI GC'GGCC_GC Cuts at: 3601 3601 Size: 6154 NspI r_CATG'y Cuts at: 1132 1229 2680 3512 5057 1132 Size: 97 1451 832 1545 2229 Fragments arranged by size: 2229 1545 1451 832 97 PasI CC'CwG_GG Cuts at: 1348 2036 2716 2966 1348 Size: 688 680 250 4536 Fragments arranged by size: 4536 688 680 250 PciI A'CATG_T Cuts at: 2676 5053 2676 Size: 2377 3777 PflMI CCAn_nnn'nTGG Cuts at: 1108 2353 2992 1108 Size: 1245 639 4270 Fragments arranged by size: 4270 1245 639 PfoI T'CCnGG_A Cuts at: 2457 2457 Size: 6154 PmeI GTTT'AAAC Cuts at: 3458 3458 Size: 6154 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3053 3085 4223 4255 5736 5768 3053 Size: 32 1138 32 1481 32 3439 Fragments arranged by size: 3439 1481 1138 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3342 3342 Size: 6154 PshAI GACnn'nnGTC Cuts at: 1613 2309 1613 Size: 696 5458 PsiI TTA'TAA Cuts at: 3724 3724 Size: 6154 PspOMI G'GGCC_C Cuts at: 2862 2862 Size: 6154 PspXI vC'TCGA_Gb Cuts at: 2191 2191 Size: 6154 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3038 3070 3038 Size: 32 6122 PstI C_TGCA'G Cuts at: 830 2490 3506 830 Size: 1660 1016 3478 Fragments arranged by size: 3478 1660 1016 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6154 PvuII CAG'CTG Cuts at: 2921 2989 3241 2921 Size: 68 252 5834 Fragments arranged by size: 5834 252 68 SacI G_AGCT'C Cuts at: 2198 2874 2943 2198 Size: 676 69 5409 Fragments arranged by size: 5409 676 69 SalI G'TCGA_C Cuts at: 1936 3418 3496 1936 Size: 1482 78 4594 Fragments arranged by size: 4594 1482 78 SapI GCTCTTCn'nnn_ Cuts at: 2693 2693 Size: 6154 SbfI CC_TGCA'GG Cuts at: 3506 3506 Size: 6154 ScaI AGT'ACT Cuts at: 4402 6114 4402 Size: 1712 4442 SfcI C'TryA_G Cuts at: 826 1045 2486 3502 4637 5318 5509 826 Size: 219 1441 1016 1135 681 191 1471 Fragments arranged by size: 1471 1441 1135 1016 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 SgrAI Cr'CCGG_yG Cuts at: 1700 2141 1700 Size: 441 5713 SmaI CCC'GGG Cuts at: 1872 2272 3026 3481 1872 Size: 400 754 455 4545 Fragments arranged by size: 4545 754 455 400 SmlI C'TyrA_G Cuts at: 820 1017 1943 2191 2780 3172 3337 4258 5159 Size: 197 926 248 589 392 165 921 901 Cuts at: 5159 5421 820 Size: 262 1553 Fragments arranged by size: 1553 926 921 901 589 392 262 248 197 165 SnaBI TAC'GTA Cuts at: 493 3945 493 Size: 3452 2702 SpeI A'CTAG_T Cuts at: 152 2434 152 Size: 2282 3872 SphI G_CATG'C Cuts at: 1132 3512 1132 Size: 2380 3774 SspI AAT'ATT Cuts at: 5 52 2518 4078 5 Size: 47 2466 1560 2081 Fragments arranged by size: 2466 2081 1560 47 StuI AGG'CCT Cuts at: 1517 3194 1517 Size: 1677 4477 StyI C'CwwG_G Cuts at: 513 1065 2169 2329 2634 3593 513 Size: 552 1104 160 305 959 3074 Fragments arranged by size: 3074 1104 959 552 305 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2162 3437 4181 4198 4351 4536 4981 Size: 1255 582 1275 744 17 153 185 445 Cuts at: 4981 325 Size: 1498 Fragments arranged by size: 1498 1275 1255 744 582 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2673 2929 3159 Size: 274 80 33 51 1443 696 256 230 Cuts at: 3159 4400 6112 96 Size: 1241 1712 138 Fragments arranged by size: 1712 1443 1241 696 274 256 230 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3326 3358 6114 6146 3326 Size: 32 2756 32 3334 Fragments arranged by size: 3334 2756 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6154 XbaI T'CTAG_A Cuts at: 3490 3490 Size: 6154 XhoI C'TCGA_G Cuts at: 1943 2191 1943 Size: 248 5906 XmaI C'CCGG_G Cuts at: 1870 2270 3024 3479 1870 Size: 400 754 455 4545 Fragments arranged by size: 4545 754 455 400 XmnI GAAnn'nnTTC Cuts at: 4283 4283 Size: 6154 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3962 276 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI