(Linear) (Six-base) MAPSORT of: KIEE0955.seq Check: 2496 from: 1 to: 1449 KIEE0955.orf With 182 enzymes: SgfI * March 22, 2012 15:49 .. AccI GT'mk_AC Cuts at: 0 1414 1449 Size: 1414 35 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1257 1449 Size: 1257 192 AfeI AGC'GCT Cuts at: 0 337 1449 Size: 337 1112 AflIII A'CryG_T Cuts at: 0 671 1449 Size: 671 778 AhdI GACnn_n'nnGTC Cuts at: 0 1410 1449 Size: 1410 39 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 448 480 1048 1080 1277 1309 1449 Size: 448 32 568 32 197 32 140 Fragments arranged by size: 568 448 197 140 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 747 1449 Size: 747 702 ApaI G_GGCC'C Cuts at: 0 861 1449 Size: 861 588 AvaI C'yCGr_G Cuts at: 0 39 186 265 595 1019 1449 Size: 39 147 79 330 424 430 Fragments arranged by size: 430 424 330 147 79 39 BanI G'GyrC_C Cuts at: 0 133 1449 Size: 133 1316 BanII G_rGCy'C Cuts at: 0 40 74 193 824 861 869 938 1449 Size: 40 34 119 631 37 8 69 511 Fragments arranged by size: 631 511 119 69 40 37 34 8 BglI GCCn_nnn'nGGC Cuts at: 0 35 110 131 1449 Size: 35 75 21 1318 Fragments arranged by size: 1318 75 35 21 Bme1580I G_kGCm'C Cuts at: 0 596 861 1351 1449 Size: 596 265 490 98 Fragments arranged by size: 596 490 265 98 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 436 525 1171 1449 Size: 436 89 646 278 Fragments arranged by size: 646 436 278 89 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 760 1152 1353 1449 Size: 760 392 201 96 Fragments arranged by size: 760 392 201 96 BsaI GGTCTCn'nnnn_ Cuts at: 0 412 1449 Size: 412 1037 BsaHI Gr'CG_yC Cuts at: 0 176 1449 Size: 176 1273 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 691 721 1048 1078 1374 1404 1449 Size: 691 30 327 30 296 30 45 Fragments arranged by size: 691 327 296 45 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 104 236 928 1316 1434 1449 Size: 104 132 692 388 118 15 Fragments arranged by size: 692 388 132 118 104 15 BseYI C'CCAG_C Cuts at: 0 276 1005 1232 1341 1449 Size: 276 729 227 109 108 Fragments arranged by size: 729 276 227 109 108 BsiEI CG_ry'CG Cuts at: 0 1418 1449 Size: 1418 31 BsiHKAI G_wGCw'C Cuts at: 0 193 869 938 1449 Size: 193 676 69 511 Fragments arranged by size: 676 511 193 69 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1307 1449 Size: 1307 142 Bsp1286I G_dGCh'C Cuts at: 0 40 74 193 596 824 861 869 938 Size: 40 34 119 403 228 37 8 69 Cuts at: 938 1351 1449 Size: 413 98 Fragments arranged by size: 413 403 228 119 98 69 40 37 34 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 755 1449 Size: 755 694 BsrBI CCG'CTC Cuts at: 0 5 1449 Size: 5 1444 BsrDI GCAATG_nn' Cuts at: 0 384 1449 Size: 384 1065 BsrFI r'CCGG_y Cuts at: 0 136 1449 Size: 136 1313 BsrGI T'GTAC_A Cuts at: 0 668 1449 Size: 668 781 BstAPI GCAn_nnn'nTGC Cuts at: 0 489 1449 Size: 489 960 BstEII G'GTnAC_C Cuts at: 0 297 1449 Size: 297 1152 BtgI C'CryG_G Cuts at: 0 164 1449 Size: 164 1285 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1113 1233 1449 Size: 1113 120 216 Fragments arranged by size: 1113 216 120 BtsI GCAGTG_nn' Cuts at: 0 504 764 801 1449 Size: 504 260 37 648 Fragments arranged by size: 648 504 260 37 ClaI AT'CG_AT Cuts at: 0 902 1449 Size: 902 547 DrdI GACnn_nn'nnGTC Cuts at: 0 769 1449 Size: 769 680 EarI CTCTTCn'nnn_ Cuts at: 0 688 1270 1449 Size: 688 582 179 Fragments arranged by size: 688 582 179 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 95 101 158 233 1449 Size: 95 6 57 75 1216 Fragments arranged by size: 1216 95 75 57 6 EcoICRI GAG'CTC Cuts at: 0 191 867 936 1449 Size: 191 676 69 513 Fragments arranged by size: 676 513 191 69 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 436 525 1171 1257 1449 Size: 436 89 646 86 192 Fragments arranged by size: 646 436 192 89 86 EcoNI CCTnn'n_nnAGG Cuts at: 0 20 1449 Size: 20 1429 EcoO109I rG'GnC_Cy Cuts at: 0 182 235 858 1337 1449 Size: 182 53 623 479 112 Fragments arranged by size: 623 479 182 112 53 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 580 1449 Size: 580 869 FspI TGC'GCA Cuts at: 0 1196 1449 Size: 1196 253 FspAI rTGC'GCAy Cuts at: 0 1196 1449 Size: 1196 253 HaeII r_GCGC'y Cuts at: 0 339 1449 Size: 339 1110 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 877 895 909 927 1065 1097 1396 1428 Size: 877 18 14 18 138 32 299 32 Cuts at: 1428 1449 Size: 21 Fragments arranged by size: 877 299 138 32 32 21 18 18 14 HincII GTy'rAC Cuts at: 0 641 1415 1449 Size: 641 774 34 Fragments arranged by size: 774 641 34 HindIII A'AGCT_T Cuts at: 0 1376 1449 Size: 1376 73 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1320 1417 1449 Size: 1320 97 32 Fragments arranged by size: 1320 97 32 MslI CAynn'nnrTG Cuts at: 0 367 1000 1449 Size: 367 633 449 Fragments arranged by size: 633 449 367 MspA1I CmG'CkG Cuts at: 0 215 230 290 916 984 1236 1345 1449 Size: 215 15 60 626 68 252 109 104 Fragments arranged by size: 626 252 215 109 104 68 60 15 NcoI C'CATG_G Cuts at: 0 164 1449 Size: 164 1285 NspI r_CATG'y Cuts at: 0 675 1449 Size: 675 774 PasI CC'CwG_GG Cuts at: 0 31 711 961 1449 Size: 31 680 250 488 Fragments arranged by size: 680 488 250 31 PciI A'CATG_T Cuts at: 0 671 1449 Size: 671 778 PflMI CCAn_nnn'nTGG Cuts at: 0 348 987 1449 Size: 348 639 462 Fragments arranged by size: 639 462 348 PfoI T'CCnGG_A Cuts at: 0 452 1449 Size: 452 997 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1048 1080 1449 Size: 1048 32 369 Fragments arranged by size: 1048 369 32 PpuMI rG'GwC_Cy Cuts at: 0 1337 1449 Size: 1337 112 PshAI GACnn'nnGTC Cuts at: 0 304 1449 Size: 304 1145 PspOMI G'GGCC_C Cuts at: 0 857 1449 Size: 857 592 PspXI vC'TCGA_Gb Cuts at: 0 186 1449 Size: 186 1263 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1033 1065 1449 Size: 1033 32 384 Fragments arranged by size: 1033 384 32 PstI C_TGCA'G Cuts at: 0 485 1449 Size: 485 964 PvuII CAG'CTG Cuts at: 0 916 984 1236 1449 Size: 916 68 252 213 Fragments arranged by size: 916 252 213 68 SacI G_AGCT'C Cuts at: 0 193 869 938 1449 Size: 193 676 69 511 Fragments arranged by size: 676 511 193 69 SalI G'TCGA_C Cuts at: 0 1413 1449 Size: 1413 36 SapI GCTCTTCn'nnn_ Cuts at: 0 688 1449 Size: 688 761 SfcI C'TryA_G Cuts at: 0 481 1449 Size: 481 968 SgrAI Cr'CCGG_yG Cuts at: 0 136 1449 Size: 136 1313 SmaI CCC'GGG Cuts at: 0 267 1021 1449 Size: 267 754 428 Fragments arranged by size: 754 428 267 SmlI C'TyrA_G Cuts at: 0 186 775 1167 1332 1449 Size: 186 589 392 165 117 Fragments arranged by size: 589 392 186 165 117 SpeI A'CTAG_T Cuts at: 0 429 1449 Size: 429 1020 SspI AAT'ATT Cuts at: 0 513 1449 Size: 513 936 StuI AGG'CCT Cuts at: 0 1189 1449 Size: 1189 260 StyI C'CwwG_G Cuts at: 0 164 324 629 1449 Size: 164 160 305 820 Fragments arranged by size: 820 305 164 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 157 1432 1449 Size: 157 1275 17 Fragments arranged by size: 1275 157 17 TatI w'GTAC_w Cuts at: 0 668 924 1154 1449 Size: 668 256 230 295 Fragments arranged by size: 668 295 256 230 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1321 1353 1449 Size: 1321 32 96 Fragments arranged by size: 1321 96 32 XhoI C'TCGA_G Cuts at: 0 186 1449 Size: 186 1263 XmaI C'CCGG_G Cuts at: 0 265 1019 1449 Size: 265 754 430 Fragments arranged by size: 754 430 265 Enzymes that do cut: AccI AcuI AfeI AflIII AhdI AloI AlwNI ApaI AvaI BanI BanII BglI Bme1580I BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BtgI BtgZI BtsI ClaI DrdI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HincII HindIII MmeI MslI MspA1I NcoI NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII SacI SalI SapI SfcI SgrAI SmaI SmlI SpeI SspI StuI StyI TaqII TatI TstI XhoI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsgI BsiWI BsmI BspEI BspHI BssHII BssSI BstBI BstXI BstYI BstZ17I Bsu36I CspCI DraI DraIII EaeI EagI EcoRI EcoRV FseI HpaI KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PsiI PvuI RsrII SacII SanDI SbfI ScaI SexAI SfiI SfoI SgfI SnaBI SphI SrfI SwaI Tth111I XbaI XcmI XmnI ZraI