(Linear) (Six-base) MAPSORT of: KIEE1560.seq Check: 6971 from: 1 to: 738 KIEE1560.orf With 182 enzymes: SgfI * July 15, 2010 18:42 .. AccI GT'mk_AC Cuts at: 0 683 738 Size: 683 55 AgeI A'CCGG_T Cuts at: 0 586 738 Size: 586 152 AleI CACnn'nnGTG Cuts at: 0 367 738 Size: 367 371 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 509 541 738 Size: 509 32 197 Fragments arranged by size: 509 197 32 AlwNI CAG_nnn'CTG Cuts at: 0 120 738 Size: 120 618 ApaI G_GGCC'C Cuts at: 0 228 614 738 Size: 228 386 124 Fragments arranged by size: 386 228 124 BamHI G'GATC_C Cuts at: 0 567 738 Size: 567 171 BanII G_rGCy'C Cuts at: 0 228 614 738 Size: 228 386 124 Fragments arranged by size: 386 228 124 BbsI GAAGACnn'nnnn_ Cuts at: 0 583 738 Size: 583 155 BglI GCCn_nnn'nGGC Cuts at: 0 381 738 Size: 381 357 Bme1580I G_kGCm'C Cuts at: 0 228 614 738 Size: 228 386 124 Fragments arranged by size: 386 228 124 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 361 659 738 Size: 361 298 79 Fragments arranged by size: 361 298 79 BsaI GGTCTCn'nnnn_ Cuts at: 0 699 738 Size: 699 39 BsaWI w'CCGG_w Cuts at: 0 586 738 Size: 586 152 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 4 70 738 Size: 4 66 668 Fragments arranged by size: 668 66 4 BseYI C'CCAG_C Cuts at: 0 46 254 437 738 Size: 46 208 183 301 Fragments arranged by size: 301 208 183 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 444 517 738 Size: 444 73 221 Fragments arranged by size: 444 221 73 BsiHKAI G_wGCw'C Cuts at: 0 460 738 Size: 460 278 BsmBI CGTCTCn'nnnn_ Cuts at: 0 526 738 Size: 526 212 Bsp1286I G_dGCh'C Cuts at: 0 228 460 614 738 Size: 228 232 154 124 Fragments arranged by size: 232 228 154 124 BsrBI CCG'CTC Cuts at: 0 249 738 Size: 249 489 BsrFI r'CCGG_y Cuts at: 0 586 738 Size: 586 152 BssSI C'ACGA_G Cuts at: 0 616 738 Size: 616 122 BstYI r'GATC_y Cuts at: 0 567 738 Size: 567 171 BtgI C'CryG_G Cuts at: 0 528 738 Size: 528 210 BtsI GCAGTG_nn' Cuts at: 0 189 432 472 738 Size: 189 243 40 266 Fragments arranged by size: 266 243 189 40 EarI CTCTTCn'nnn_ Cuts at: 0 124 133 529 738 Size: 124 9 396 209 Fragments arranged by size: 396 209 124 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 361 659 738 Size: 361 298 79 Fragments arranged by size: 361 298 79 EcoO109I rG'GnC_Cy Cuts at: 0 224 610 611 738 Size: 224 386 1 127 Fragments arranged by size: 386 224 127 1 HaeII r_GCGC'y Cuts at: 0 735 738 Size: 735 3 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 171 203 671 703 738 Size: 171 32 468 32 35 Fragments arranged by size: 468 171 35 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 115 341 738 Size: 115 226 397 Fragments arranged by size: 397 226 115 MslI CAynn'nnrTG Cuts at: 0 157 367 738 Size: 157 210 371 Fragments arranged by size: 371 210 157 MspA1I CmG'CkG Cuts at: 0 184 339 367 420 738 Size: 184 155 28 53 318 Fragments arranged by size: 318 184 155 53 28 NcoI C'CATG_G Cuts at: 0 528 738 Size: 528 210 PasI CC'CwG_GG Cuts at: 0 40 738 Size: 40 698 PfoI T'CCnGG_A Cuts at: 0 675 738 Size: 675 63 PspOMI G'GGCC_C Cuts at: 0 224 610 738 Size: 224 386 128 Fragments arranged by size: 386 224 128 PstI C_TGCA'G Cuts at: 0 338 479 738 Size: 338 141 259 Fragments arranged by size: 338 259 141 PvuII CAG'CTG Cuts at: 0 184 339 367 420 738 Size: 184 155 28 53 318 Fragments arranged by size: 318 184 155 53 28 SfcI C'TryA_G Cuts at: 0 334 475 738 Size: 334 141 263 Fragments arranged by size: 334 263 141 StyI C'CwwG_G Cuts at: 0 218 528 636 738 Size: 218 310 108 102 Fragments arranged by size: 310 218 108 102 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 129 161 496 528 738 Size: 129 32 335 32 210 Fragments arranged by size: 335 210 129 32 32 Enzymes that do cut: AccI AgeI AleI AloI AlwNI ApaI BamHI BanII BbsI BglI Bme1580I BpmI BsaI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BssSI BstYI BtgI BtsI EarI Eco57MI EcoO109I HaeII Hin4I MmeI MslI MspA1I NcoI PasI PfoI PspOMI PstI PvuII SfcI StyI TstI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AflIII AhdI AlfI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI