################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccIII AciI AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AviII AvrII BalI BamHI BanII BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BlnI BpmI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HapII HincII HindII HindIII HpaI HpaII KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tru9I Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 142 CGCG CG^CG AcyI 82 GGCGCC G[GA]^CG[CT]C AluI 269 AGCT AG^CT AlwI 232 GATCC GGATC(4/5) AlwNI 99 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 150 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 97 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 180 CCCGAG C^[CT]CG[GA]G AvaII 164 GGACC G^G[AT]CC BanI 82 GGCGCC G^G[CT][GA]CC BbeI 82 GGCGCC GGCGC^C BbsI 94 GAAGAC GAAGAC(2/6) BbvI 107 GCTGC GCAGC(8/12) BglI 74 GCCCCTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 221 AGATCT A^GATCT BlpI 270 GCTGAGC GC^T[ACGT]AGC BmyI 241 GTGCCC G[AGT]GC[ACT]^C Bpu1102I 270 GCTGAGC GC^T[ACGT]AGC BpuAI 94 GAAGAC GAAGAC(2/6) BsaHI 82 GGCGCC G[GA]^CG[CT]C BsgI 17 CTGCAC GTGCAG(16/14) BsoBI 180 CCCGAG C^[CT]CG[GA]G Bsp1286I 241 GTGCCC G[AGT]GC[ACT]^C BsrI 161 ACTGG ACTGG(1/-1) BstUI 142 CGCG CG^CG BstYI 221 AGATCT [GA]^GATC[CT] CelII 270 GCTGAGC GC^T[ACGT]AGC DraII 72 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoNI 176 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 72 AGGCCCC [GA]G^G[ACGT]CC[CT] Fnu4HI 107 GCTGC GC^[ACGT]GC HgaI 101 GCGTC GACGC(5/10) Hin1I 82 GGCGCC G[GA]^CG[CT]C ItaI 107 GCTGC GC^[ACGT]GC KasI 82 GGCGCC G^GCGCC MaeII 119 ACGT A^CGT MaeIII 125 GTTAC ^GT[ACGT]AC MflI 221 AGATCT [GA]^GATC[CT] MvnI 142 CGCG CG^CG NarI 82 GGCGCC GG^CGCC NlaIII 90 CATG CATG^ PflFI 97 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 172 GACTC GAGTC(4/5) SfcI 10 CTACAG C^T[GA][CT]AG SfoI 82 GGCGCC GGC^GCC StuI 184 AGGCCT AGG^CCT TfiI 258 GATTC G^A[AT]TC TseI 107 GCTGC G^C[AT]GC Tth111I 97 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 221 AGATCT [GA]^GATC[CT] XmnI 150 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### Cac8I 4 GCTCGC GC[ACGT]^[ACGT]GC 70 GCAGGC Cfr13I 73 GGCCC G^G[ACGT]CC 164 GGACC DpnII 222 GATC ^GATC 232 GATC HaeII 51 GGCGCT [GA]GCGC^[CT] 82 GGCGCC HaeIII 73 GGCC GG^CC 185 GGCC HinfI 172 GACTC G^A[ACGT]TC 258 GATTC HphI 147 GGTGA GGTGA(8/7) 249 GGTGA MboI 222 GATC ^GATC 232 GATC MboII 94 GAAGA GAAGA(8/7) 252 GAAGA NdeII 222 GATC ^GATC 232 GATC NlaIV 73 GGCCCC GG[ACGT]^[ACGT]CC 82 GGCGCC Sau3AI 222 GATC ^GATC 232 GATC Sau96I 73 GGCCC G^G[ACGT]CC 164 GGACC ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### BsaJI BsiYI BslI BssKI BstNI CfoI DdeI EcoRII HhaI HinP1I MnlI MvaI MwoI ScrFI