(Circular) (Six-base) MAPSORT of: pFN21AE1469.seq Check: 6034 from: 1 to: 4984 pFN21AE1469 4984 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2794 278 Size: 53 83 186 936 1258 2468 Fragments arranged by size: 2468 1258 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2327 1937 Size: 390 4594 Acc65I G'GTAC_C Cuts at: 2305 2305 Size: 4984 AclI AA'CG_TT Cuts at: 1563 3111 3484 1563 Size: 1548 373 3063 Fragments arranged by size: 3063 1548 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2197 3047 4431 4699 1632 Size: 565 850 1384 268 1917 Fragments arranged by size: 1917 1384 850 565 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4787 AflIII A'CryG_T Cuts at: 1203 3883 4860 1203 Size: 2680 977 1327 Fragments arranged by size: 2680 1327 977 AgeI A'CCGG_T Cuts at: 3815 3815 Size: 4984 AhdI GACnn_n'nnGTC Cuts at: 3713 3713 Size: 4984 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4984 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4984 AlwNI CAG_nnn'CTG Cuts at: 2109 2727 4299 2109 Size: 618 1572 2794 Fragments arranged by size: 2794 1572 618 ApaLI G'TGCA_C Cuts at: 3041 4197 3041 Size: 1156 3828 ApoI r'AATT_y Cuts at: 1493 2293 2519 2671 4780 1493 Size: 800 226 152 2109 1697 Fragments arranged by size: 2109 1697 800 226 152 AseI AT'TA_AT Cuts at: 160 1032 3538 160 Size: 872 2506 1606 Fragments arranged by size: 2506 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4984 AvaI C'yCGr_G Cuts at: 1870 1943 2185 2309 1870 Size: 73 242 124 4545 Fragments arranged by size: 4545 242 124 73 BamHI G'GATC_C Cuts at: 2314 2681 2314 Size: 367 4617 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2087 2305 3760 618 Size: 325 217 627 300 218 1455 1842 Fragments arranged by size: 1842 1455 627 325 300 218 217 BanII G_rGCy'C Cuts at: 1391 2303 1391 Size: 912 4072 BbeI G_GCGC'C Cuts at: 2091 2091 Size: 4984 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2106 928 Size: 971 207 3806 Fragments arranged by size: 3806 971 207 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3175 3209 1903 Size: 34 1238 34 3678 Fragments arranged by size: 3678 1238 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2876 4086 2876 Size: 1210 3774 BclI T'GATC_A Cuts at: 1548 1548 Size: 4984 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2085 3595 136 Size: 107 122 71 631 605 413 1510 1525 Fragments arranged by size: 1525 1510 631 605 413 122 107 71 BglII A'GATC_T Cuts at: 2226 4979 2226 Size: 2753 2231 BlpI GC'TnA_GC Cuts at: 2276 2401 2276 Size: 125 4859 Bme1580I G_kGCm'C Cuts at: 1790 2250 3045 4201 1790 Size: 460 795 1156 2573 Fragments arranged by size: 2573 1156 795 460 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4984 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2715 3673 442 Size: 416 53 83 399 24 1298 958 1753 Fragments arranged by size: 1753 1298 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4984 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3644 1239 Size: 2405 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3109 3974 4272 3109 Size: 865 298 3821 Fragments arranged by size: 3821 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3647 882 Size: 617 2148 2219 Fragments arranged by size: 2219 2148 617 BsaAI yAC'GTr Cuts at: 493 1204 2775 493 Size: 711 1571 2702 Fragments arranged by size: 2702 1571 711 BsaBI GATnn'nnATC Cuts at: 4978 4978 Size: 4984 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2088 2791 3173 3808 Size: 53 83 186 936 555 703 382 635 Cuts at: 3808 275 Size: 1451 Fragments arranged by size: 1451 936 703 635 555 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3417 3815 4089 4236 4647 3417 Size: 398 274 147 411 3754 Fragments arranged by size: 3754 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4759 Fragments arranged by size: 4759 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4984 BseYI C'CCAG_C Cuts at: 4187 4683 4187 Size: 496 4488 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2007 1163 Size: 573 271 4140 Fragments arranged by size: 4140 573 271 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2434 3195 4223 1540 Size: 275 186 433 761 1028 2301 Fragments arranged by size: 2301 1028 761 433 275 186 BsiHKAI G_wGCw'C Cuts at: 3045 3130 4201 3045 Size: 85 1071 3828 Fragments arranged by size: 3828 1071 85 BsmI GAATG_Cn' Cuts at: 2495 2588 2495 Size: 93 4891 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2250 2303 3045 3130 4201 1391 Size: 399 460 53 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 460 399 85 53 BspEI T'CCGG_A Cuts at: 4647 4647 Size: 4984 BspHI T'CATG_A Cuts at: 2873 4603 2873 Size: 1730 3254 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2339 844 Size: 1495 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2871 1394 Size: 429 1048 3507 Fragments arranged by size: 3507 1048 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3479 3653 65 Size: 1183 2231 174 1396 Fragments arranged by size: 2231 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3628 3815 1700 Size: 110 1818 187 2869 Fragments arranged by size: 2869 1818 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4984 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4984 BssSI C'ACGA_G Cuts at: 3044 4056 3044 Size: 1012 3972 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4984 BstBI TT'CG_AA Cuts at: 3788 3788 Size: 4984 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4984 BstYI r'GATC_y Cuts at: 1972 2226 2314 2681 3066 3083 4535 4621 4633 Size: 254 88 367 385 17 1452 86 12 Cuts at: 4633 4979 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 254 88 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3493 Fragments arranged by size: 3493 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4245 Fragments arranged by size: 4245 683 56 BtsI GCAGTG_nn' Cuts at: 2500 3294 3314 2500 Size: 794 20 4170 Fragments arranged by size: 4170 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4984 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4949 DraI TTT'AAA Cuts at: 2288 2288 Size: 4984 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3991 809 Size: 507 2675 1802 Fragments arranged by size: 2675 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2431 3320 4949 Size: 54 1425 50 275 18 601 889 1629 Cuts at: 4949 8 Size: 43 Fragments arranged by size: 1629 1425 889 601 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2431 1537 Size: 275 619 4090 Fragments arranged by size: 4090 619 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2914 1353 Size: 1561 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3571 3945 4091 387 Size: 1064 138 1982 374 146 1280 Fragments arranged by size: 1982 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2197 3047 3644 4431 4699 1239 Size: 393 565 850 597 787 268 1524 Fragments arranged by size: 1524 850 787 597 565 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2185 2185 Size: 4984 EcoO109I rG'GnC_Cy Cuts at: 2078 2078 Size: 4984 EcoRI G'AATT_C Cuts at: 2293 2671 2293 Size: 378 4606 FspI TGC'GCA Cuts at: 3490 3490 Size: 4984 HaeII r_GCGC'y Cuts at: 1678 2060 2091 4131 1678 Size: 382 31 2040 2531 Fragments arranged by size: 2531 2040 382 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3625 3657 3699 3731 1373 Size: 32 135 32 2053 32 42 32 2626 Fragments arranged by size: 2626 2053 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2328 2574 669 Size: 988 281 390 246 3079 Fragments arranged by size: 3079 988 390 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4984 HpaI GTT'AAC Cuts at: 2574 2574 Size: 4984 KasI G'GCGC_C Cuts at: 2087 2087 Size: 4984 KpnI G_GTAC'C Cuts at: 2309 2309 Size: 4984 MfeI C'AATT_G Cuts at: 2583 2583 Size: 4984 MluI A'CGCG_T Cuts at: 4860 4860 Size: 4984 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4098 4282 1305 Size: 2793 184 2007 Fragments arranged by size: 2793 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3162 Fragments arranged by size: 3162 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2942 3301 3460 518 Size: 648 1776 359 159 2042 Fragments arranged by size: 2042 1776 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2400 3077 4225 4470 1606 Size: 794 677 1148 245 2120 Fragments arranged by size: 2120 1148 794 677 245 NarI GG'CG_CC Cuts at: 2088 2088 Size: 4984 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3493 Fragments arranged by size: 3493 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4984 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4984 NotI GC'GGCC_GC Cuts at: 2431 2431 Size: 4984 NspI r_CATG'y Cuts at: 1132 1229 2342 3887 1132 Size: 97 1113 1545 2229 Fragments arranged by size: 2229 1545 1113 97 PasI CC'CwG_GG Cuts at: 1348 2250 1348 Size: 902 4082 PciI A'CATG_T Cuts at: 3883 3883 Size: 4984 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4984 PmeI GTTT'AAAC Cuts at: 2288 2288 Size: 4984 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3053 3085 4566 4598 3053 Size: 32 1481 32 3439 Fragments arranged by size: 3439 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4984 PsiI TTA'TAA Cuts at: 2554 2554 Size: 4984 PstI C_TGCA'G Cuts at: 830 2336 830 Size: 1506 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4984 SalI G'TCGA_C Cuts at: 1936 2326 1936 Size: 390 4594 SbfI CC_TGCA'GG Cuts at: 2336 2336 Size: 4984 ScaI AGT'ACT Cuts at: 3232 4944 3232 Size: 1712 3272 SfcI C'TryA_G Cuts at: 826 1045 2015 2332 3467 4148 4339 826 Size: 219 970 317 1135 681 191 1471 Fragments arranged by size: 1471 1135 970 681 317 219 191 SfoI GGC'GCC Cuts at: 2089 2089 Size: 4984 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4984 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4984 SmaI CCC'GGG Cuts at: 1872 2311 1872 Size: 439 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3088 3989 4251 820 Size: 197 926 1145 901 262 1553 Fragments arranged by size: 1553 1145 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2775 493 Size: 2282 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4984 SphI G_CATG'C Cuts at: 1132 2342 1132 Size: 1210 3774 SspI AAT'ATT Cuts at: 5 52 2908 5 Size: 47 2856 2081 Fragments arranged by size: 2856 2081 47 StuI AGG'CCT Cuts at: 1517 2191 1517 Size: 674 4310 StyI C'CwwG_G Cuts at: 513 1065 2004 2423 513 Size: 552 939 419 3074 Fragments arranged by size: 3074 939 552 419 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2242 3011 3028 3181 3366 3811 325 Size: 1255 662 769 17 153 185 445 1498 Fragments arranged by size: 1498 1255 769 662 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3230 4942 96 Size: 274 80 33 51 1443 1253 1712 138 Fragments arranged by size: 1712 1443 1253 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4944 4976 4944 Size: 32 4952 Tth111I GACn'n_nGTC Cuts at: 1316 2105 1316 Size: 789 4195 XbaI T'CTAG_A Cuts at: 2320 2320 Size: 4984 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4984 XmaI C'CCGG_G Cuts at: 1870 2309 1870 Size: 439 4545 XmnI GAAnn'nnTTC Cuts at: 2159 3113 2159 Size: 954 4030 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2792 276 Size: 53 83 186 936 1258 2468 Fragments arranged by size: 2468 1258 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI