################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AcsI AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsiEI BsiWI BsmBI BsmFI BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HindIII KasI KpnI KspI MamI MfeI MflI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 320 TCCGGA T^CCGGA AclI 1224 AACGTT AA^CGTT AhdI 1203 GACCGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 600 GATCC GGATC(4/5) ApaI 420 GGGCCC GGGCC^C Asp700I 1006 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1203 GACCGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbsI 19 GTCTTC GAAGAC(2/6) BcgI 402 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 442 CTAG C^TAG BpmI 187 CTGGAG CTGGAG(16/14) BpuAI 19 GTCTTC GAAGAC(2/6) BsaAI 181 TACGTG [CT]AC^GT[GA] BsaI 586 GAGACC GGTCTC(1/5) BseAI 320 TCCGGA T^CCGGA BsgI 1315 GTGCAG GTGCAG(16/14) Bsp1407I 104 TGTACA T^GTACA BspEI 320 TCCGGA T^CCGGA BsrGI 104 TGTACA T^GTACA BssSI 214 CACGAG CACGAG(-5/-1) BstXI 556 CCAGACAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Eam1105I 1203 GACCGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1002 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 99 GACGC GACGC(5/10) HincII 1231 GTTAAC GT[CT]^[GA]AC HindII 1231 GTTAAC GT[CT]^[GA]AC HpaI 1231 GTTAAC GTT^AAC MaeI 442 CTAG C^TAG MroI 320 TCCGGA T^CCGGA NcoI 1116 CCATGG C^CATGG NspI 251 ACATGC [GA]CATG^[CT] PleI 1301 GAGTC GAGTC(4/5) PpuMI 1209 GGGTCCT [GA]G^G[AT]CC[CT] Psp1406I 1224 AACGTT AA^CGTT SacI 1280 GAGCTC GAGCT^C SapI 747 GCTCTTC GCTCTTC(1/4) SmaI 590 CCCGGG CCC^GGG SmlI 1259 CTCAAG C^T[CT][GA]AG SspBI 104 TGTACA T^GTACA TfiI 209 GAATC G^A[AT]TC XmaCI 590 CCCGGG C^CCGGG XmaI 590 CCCGGG C^CCGGG XmnI 1006 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 142 GTCTAC GT^[AC][GT]AC 769 GTCTAC AfaI 105 GTAC GT^AC 525 GTAC AspHI 691 GAGCAC G[AT]GC[AT]^C 1280 GAGCTC AvaI 583 CCCGAG C^[CT]CG[GA]G 590 CCCGGG BalI 662 TGGCCA TGG^CCA 761 TGGCCA BsaWI 320 TCCGGA [AT]^CCGG[AT] 602 TCCGGT BsiHKAI 691 GAGCAC G[AT]GC[AT]^C 1280 GAGCTC BsoBI 583 CCCGAG C^[CT]CG[GA]G 590 CCCGGG BspMI 8 ACCTGC ACCTGC(4/8) 545 ACCTGC BsrI 563 ACTGG ACTGG(1/-1) 996 CCAGT BstEII 60 GGTGACC G^GT[ACGT]ACC 1111 GGTGACC BstPI 60 GGTGACC G^GT[ACGT]ACC 1111 GGTGACC EaeI 662 TGGCCA [CT]^GGCC[GA] 761 TGGCCA EcoO65I 60 GGTGACC G^GT[ACGT]ACC 1111 GGTGACC FauI 138 GCGGG CCCGC(4/6) 963 GCGGG HinfI 209 GAATC G^A[ACGT]TC 1301 GAGTC MaeII 182 ACGT A^CGT 1225 ACGT MluNI 662 TGGCCA TGG^CCA 761 TGGCCA MscI 662 TGGCCA TGG^CCA 761 TGGCCA MslI 76 CACCGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 782 CACATGGATG PflMI 753 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 996 CCAGTTCCTGG PstI 289 CTGCAG CTGCA^G 1357 CTGCAG PvuII 292 CAGCTG CAG^CTG 1354 CAGCTG RsaI 105 GTAC GT^AC 525 GTAC TspRI 1083 CAGTG CA[GC]TG[ACGT][ACGT]^ 1155 CACTG Van91I 753 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 996 CCAGTTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AccII AciI AluI AlwNI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfcI StyI Tru9I TseI Tsp45I Tsp509I