(Circular) (Six-base) MAPSORT of: pFN21AE1563.seq Check: 4612 from: 1 to: 6079 pFN21AE1563 6079 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2559 2559 Size: 6079 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3889 278 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2148 2775 3422 1937 Size: 211 627 647 4594 Fragments arranged by size: 4594 647 627 211 Acc65I G'GTAC_C Cuts at: 3400 3400 Size: 6079 AclI AA'CG_TT Cuts at: 1563 3230 4206 4579 1563 Size: 1667 976 373 3063 Fragments arranged by size: 3063 1667 976 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3096 4142 5526 5794 1632 Size: 1464 1046 1384 268 1917 Fragments arranged by size: 1917 1464 1384 1046 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5882 AflIII A'CryG_T Cuts at: 1203 4978 5955 1203 Size: 3775 977 1327 Fragments arranged by size: 3775 1327 977 AgeI A'CCGG_T Cuts at: 4910 4910 Size: 6079 AhdI GACnn_n'nnGTC Cuts at: 3213 4808 3213 Size: 1595 4484 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6079 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2894 1184 Size: 1710 4369 AlwNI CAG_nnn'CTG Cuts at: 2764 3007 3179 3203 3822 5394 2764 Size: 243 172 24 619 1572 3449 Fragments arranged by size: 3449 1572 619 243 172 24 ApaI G_GGCC'C Cuts at: 2429 2429 Size: 6079 ApaLI G'TGCA_C Cuts at: 4136 5292 4136 Size: 1156 4923 ApoI r'AATT_y Cuts at: 1493 3388 3614 3766 5875 1493 Size: 1895 226 152 2109 1697 Fragments arranged by size: 2109 1895 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4633 160 Size: 872 3601 1606 Fragments arranged by size: 3601 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 2588 2595 3404 1870 Size: 73 645 7 809 4545 Fragments arranged by size: 4545 809 645 73 7 BamHI G'GATC_C Cuts at: 3409 3776 3409 Size: 367 5712 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3400 4855 618 Size: 325 217 627 1613 1455 1842 Fragments arranged by size: 1842 1613 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2226 2429 3289 3398 1391 Size: 835 203 860 109 4072 Fragments arranged by size: 4072 860 835 203 109 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2017 928 Size: 971 118 4990 Fragments arranged by size: 4990 971 118 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2396 2430 4270 4304 1903 Size: 34 459 34 1840 34 3678 Fragments arranged by size: 3678 1840 459 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3971 5181 3971 Size: 1210 4869 BclI T'GATC_A Cuts at: 1548 1548 Size: 6079 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4690 136 Size: 107 122 71 631 605 3018 1525 Fragments arranged by size: 3018 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6074 6074 Size: 6079 BlpI GC'TnA_GC Cuts at: 3496 3496 Size: 6079 Bme1580I G_kGCm'C Cuts at: 1790 2429 4140 5296 1790 Size: 639 1711 1156 2573 Fragments arranged by size: 2573 1711 1156 639 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6079 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3810 4768 442 Size: 416 53 83 399 24 2393 958 1753 Fragments arranged by size: 2393 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6079 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3303 3303 Size: 6079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 4739 1239 Size: 974 2526 2579 Fragments arranged by size: 2579 2526 974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3249 4204 5069 5367 3249 Size: 955 865 298 3961 Fragments arranged by size: 3961 955 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2585 4742 882 Size: 617 1086 2157 2219 Fragments arranged by size: 2219 2157 1086 617 BsaAI yAC'GTr Cuts at: 493 1204 2188 3870 493 Size: 711 984 1682 2702 Fragments arranged by size: 2702 1682 984 711 BsaBI GATnn'nnATC Cuts at: 6073 6073 Size: 6079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3886 4268 4903 275 Size: 53 83 186 936 2353 382 635 1451 Fragments arranged by size: 2353 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2325 2607 4512 4910 5184 5331 5742 2325 Size: 282 1905 398 274 147 411 2662 Fragments arranged by size: 2662 1905 411 398 282 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2297 2327 2646 2676 1178 Size: 30 165 30 894 30 319 30 4581 Fragments arranged by size: 4581 894 319 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2333 2972 3295 1186 Size: 841 306 639 323 3970 Fragments arranged by size: 3970 841 639 323 306 BseYI C'CCAG_C Cuts at: 2977 3290 5282 5778 2977 Size: 313 1992 496 3278 Fragments arranged by size: 3278 1992 496 313 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3341 1163 Size: 573 1605 3901 Fragments arranged by size: 3901 1605 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3529 4290 5318 1540 Size: 275 186 1528 761 1028 2301 Fragments arranged by size: 2301 1528 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2700 3289 4140 4225 5296 2700 Size: 589 851 85 1071 3483 Fragments arranged by size: 3483 1071 851 589 85 BsmI GAATG_Cn' Cuts at: 2132 2370 2883 3590 3683 2132 Size: 238 513 707 93 4528 Fragments arranged by size: 4528 707 513 238 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2429 2700 3289 3398 4140 4225 Size: 399 436 203 271 589 109 742 85 Cuts at: 4225 5296 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 589 436 399 271 203 109 85 BspEI T'CCGG_A Cuts at: 2325 5742 2325 Size: 3417 2662 BspHI T'CATG_A Cuts at: 3968 5698 3968 Size: 1730 4349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2022 2559 3434 844 Size: 1178 537 875 3489 Fragments arranged by size: 3489 1178 875 537 BsrBI CCG'CTC Cuts at: 1394 1823 3966 1394 Size: 429 2143 3507 Fragments arranged by size: 3507 2143 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4574 4748 65 Size: 1183 3326 174 1396 Fragments arranged by size: 3326 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4723 4910 1700 Size: 110 2913 187 2869 Fragments arranged by size: 2913 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2109 96 Size: 2013 4066 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6079 BssSI C'ACGA_G Cuts at: 2219 4139 5151 2219 Size: 1920 1012 3147 Fragments arranged by size: 3147 1920 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6079 BstBI TT'CG_AA Cuts at: 4883 4883 Size: 6079 BstEII G'GTnAC_C Cuts at: 1184 2065 3116 1184 Size: 881 1051 4147 Fragments arranged by size: 4147 1051 881 BstXI CCAn_nnnn'nTGG Cuts at: 2568 2568 Size: 6079 BstYI r'GATC_y Cuts at: 1972 3409 3776 4161 4178 5630 5716 5728 6074 Size: 1437 367 385 17 1452 86 12 346 Cuts at: 6074 1972 Size: 1977 Fragments arranged by size: 1977 1452 1437 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2650 3121 3210 513 Size: 552 1585 471 89 3382 Fragments arranged by size: 3382 1585 552 471 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5340 Fragments arranged by size: 5340 683 56 BtsI GCAGTG_nn' Cuts at: 3595 4389 4409 3595 Size: 794 20 5265 Fragments arranged by size: 5265 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2454 2489 529 Size: 35 1890 35 4119 Fragments arranged by size: 4119 1890 35 35 DraI TTT'AAA Cuts at: 3383 3383 Size: 6079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5086 809 Size: 507 3770 1802 Fragments arranged by size: 3770 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2667 2766 3526 Size: 54 1425 50 275 18 837 99 760 Cuts at: 3526 4415 6044 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 837 760 275 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3526 1537 Size: 275 1714 4090 Fragments arranged by size: 4090 1714 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2051 2759 2949 4009 1353 Size: 698 708 190 1060 3423 Fragments arranged by size: 3423 1060 708 698 190 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2705 4666 5040 5186 387 Size: 1064 138 1116 1961 374 146 1280 Fragments arranged by size: 1961 1280 1116 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3287 3287 Size: 6079 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 3096 4142 4739 5526 5794 1239 Size: 393 581 883 1046 597 787 268 1524 Fragments arranged by size: 1524 1046 883 787 597 581 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3011 3011 Size: 6079 EcoO109I rG'GnC_Cy Cuts at: 2425 2537 3215 3371 2425 Size: 112 678 156 5133 Fragments arranged by size: 5133 678 156 112 EcoRI G'AATT_C Cuts at: 3388 3766 3388 Size: 378 5701 FspI TGC'GCA Cuts at: 4585 4585 Size: 6079 HaeII r_GCGC'y Cuts at: 1678 5226 1678 Size: 3548 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 3199 3231 4720 Size: 32 135 32 431 32 1164 32 1489 Cuts at: 4720 4752 4794 4826 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1489 1164 431 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3238 3423 3669 669 Size: 988 281 1300 185 246 3079 Fragments arranged by size: 3079 1300 988 281 246 185 HindIII A'AGCT_T Cuts at: 748 748 Size: 6079 HpaI GTT'AAC Cuts at: 3238 3669 3238 Size: 431 5648 KpnI G_GTAC'C Cuts at: 3404 3404 Size: 6079 MfeI C'AATT_G Cuts at: 3678 3678 Size: 6079 MluI A'CGCG_T Cuts at: 5955 5955 Size: 6079 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2283 2789 5193 5377 1305 Size: 978 506 2404 184 2007 Fragments arranged by size: 2404 2007 978 506 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2669 2768 10 Size: 54 1425 343 837 99 3321 Fragments arranged by size: 3321 1425 837 343 99 54 MslI CAynn'nnrTG Cuts at: 518 1166 2085 2791 4037 4396 4555 518 Size: 648 919 706 1246 359 159 2042 Fragments arranged by size: 2042 1246 919 706 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2299 2968 3361 3495 4172 5320 5565 1606 Size: 693 669 393 134 677 1148 245 2120 Fragments arranged by size: 2120 1148 693 677 669 393 245 134 NcoI C'CATG_G Cuts at: 513 1065 3121 513 Size: 552 2056 3471 Fragments arranged by size: 3471 2056 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6079 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6079 NotI GC'GGCC_GC Cuts at: 3526 3526 Size: 6079 NspI r_CATG'y Cuts at: 1132 1229 2260 3437 4982 1132 Size: 97 1031 1177 1545 2229 Fragments arranged by size: 2229 1545 1177 1031 97 PasI CC'CwG_GG Cuts at: 1348 2422 1348 Size: 1074 5005 PciI A'CATG_T Cuts at: 4978 4978 Size: 6079 PflMI CCAn_nnn'nTGG Cuts at: 1108 2764 3007 1108 Size: 1656 243 4180 Fragments arranged by size: 4180 1656 243 PfoI T'CCnGG_A Cuts at: 2358 3006 2358 Size: 648 5431 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4148 4180 5661 5693 4148 Size: 32 1481 32 4534 Fragments arranged by size: 4534 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3215 3215 Size: 6079 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6079 PsiI TTA'TAA Cuts at: 3649 3649 Size: 6079 PspOMI G'GGCC_C Cuts at: 2425 2425 Size: 6079 PstI C_TGCA'G Cuts at: 830 2298 3366 3431 830 Size: 1468 1068 65 3478 Fragments arranged by size: 3478 1468 1068 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6079 PvuII CAG'CTG Cuts at: 2299 3361 2299 Size: 1062 5017 SacI G_AGCT'C Cuts at: 3289 3289 Size: 6079 SalI G'TCGA_C Cuts at: 1936 3421 1936 Size: 1485 4594 SapI GCTCTTCn'nnn_ Cuts at: 2759 2759 Size: 6079 SbfI CC_TGCA'GG Cuts at: 3431 3431 Size: 6079 ScaI AGT'ACT Cuts at: 4327 6039 4327 Size: 1712 4367 SfcI C'TryA_G Cuts at: 826 1045 2149 2294 3362 3427 4562 5243 5434 Size: 219 1104 145 1068 65 1135 681 191 Cuts at: 5434 826 Size: 1471 Fragments arranged by size: 1471 1135 1104 1068 681 219 191 145 65 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6079 SmaI CCC'GGG Cuts at: 1872 2597 3406 1872 Size: 725 809 4545 Fragments arranged by size: 4545 809 725 SmlI C'TyrA_G Cuts at: 820 1017 1943 3264 4183 5084 5346 820 Size: 197 926 1321 919 901 262 1553 Fragments arranged by size: 1553 1321 926 919 901 262 197 SnaBI TAC'GTA Cuts at: 493 3870 493 Size: 3377 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6079 SphI G_CATG'C Cuts at: 1132 3437 1132 Size: 2305 3774 SspI AAT'ATT Cuts at: 5 52 4003 5 Size: 47 3951 2081 Fragments arranged by size: 3951 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6079 StyI C'CwwG_G Cuts at: 513 1065 2226 2769 2803 3121 3518 513 Size: 552 1161 543 34 318 397 3074 Fragments arranged by size: 3074 1161 552 543 397 318 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4106 4123 4276 4461 4906 325 Size: 1255 2526 17 153 185 445 1498 Fragments arranged by size: 2526 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2109 4325 6037 Size: 274 80 33 51 1443 132 2216 1712 Cuts at: 6037 96 Size: 138 Fragments arranged by size: 2216 1712 1443 274 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2297 2329 2646 2678 6039 6071 2297 Size: 32 317 32 3361 32 2305 Fragments arranged by size: 3361 2305 317 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6079 XbaI T'CTAG_A Cuts at: 3415 3415 Size: 6079 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6079 XmaI C'CCGG_G Cuts at: 1870 2595 3404 1870 Size: 725 809 4545 Fragments arranged by size: 4545 809 725 XmnI GAAnn'nnTTC Cuts at: 3015 4208 3015 Size: 1193 4886 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3887 276 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI