Restriction map for
pFN21AB4961
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AclI
AfaI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BcnI BfaI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BsaAI BsaBI BsaI BseRI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmI Bsp1286I Bsp1407I BspDI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DdeI DraII DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HhaI HinP1I HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeIII MamI MfeI MluI MluNI
MscI MslI MspA1I MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tsp45I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 242 TCCGGA T^CCGGA
AcyI 271 GACGCC G[GA]^CG[CT]C
AflIII 147 ACGTGT A^C[GA][CT]GT
AlwI 178 GGATC GGATC(4/5)
AvaII 169 GGACC G^G[AT]CC
BbvI 490 GCAGC GCAGC(8/12)
BclI 26 TGATCA T^GATCA
BsaHI 271 GACGCC G[GA]^CG[CT]C
BsaWI 242 TCCGGA [AT]^CCGG[AT]
BseAI 242 TCCGGA T^CCGGA
BsgI 488 GTGCAG GTGCAG(16/14)
BsmBI 269 GAGACG CGTCTC(1/5)
BspEI 242 TCCGGA T^CCGGA
BspHI 542 TCATGA T^CATGA
BsrBI 235 GAGCGG CCGCTC(-3/-3)
BssKI 36 CCTGG ^CC[ACGT]GG
BstNI 36 CCTGG CC^[AT]GG
BstYI 178 GGATCT [GA]^GATC[CT]
DraI 196 TTTAAA TTT^AAA
DsaI 275 CCATGG C^C[GA][CT]GG
EcoRII 36 CCTGG ^CC[AT]GG
EcoT14I 275 CCATGG C^C[AT][AT]GG
FauI 226 GCGGG CCCGC(4/6)
FbaI 26 TGATCA T^GATCA
Fnu4HI 490 GCAGC GC^[ACGT]GC
HaeIII 39 GGCC GG^CC
HapII 243 CCGG C^CGG
HgaI 271 GACGC GACGC(5/10)
Hin1I 271 GACGCC G[GA]^CG[CT]C
HincII 308 GTCAAC GT[CT]^[GA]AC
HindII 308 GTCAAC GT[CT]^[GA]AC
HpaII 243 CCGG C^CGG
ItaI 490 GCAGC GC^[ACGT]GC
MaeII 147 ACGT A^CGT
MflI 178 GGATCT [GA]^GATC[CT]
MroI 242 TCCGGA T^CCGGA
MspI 243 CCGG C^CGG
MvaI 36 CCTGG CC^[AT]GG
MwoI 204 GCATCTTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 275 CCATGG C^CATGG
NlaIV 239 GGTTCC GG[ACGT]^[ACGT]CC
PleI 246 GAGTC GAGTC(4/5)
RcaI 542 TCATGA T^CATGA
ScrFI 36 CCTGG CC^[ACGT]GG
SfaNI 204 GCATC GCATC(5/9)
StyI 275 CCATGG C^C[AT][AT]GG
TfiI 49 GATTC G^A[AT]TC
TseI 490 GCAGC G^C[AT]GC
XhoII 178 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 226 GCGG CCGC(-3/-1)
237 GCGG
AlwNI 330 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
491 CAGCAACTG
AseI 288 ATTAAT AT^TAAT
387 ATTAAT
AsnI 288 ATTAAT AT^TAAT
387 ATTAAT
AvaI 43 CTCGGG C^[CT]CG[GA]G
443 CTCGGG
BbsI 63 GAAGAC GAAGAC(2/6)
570 GAAGAC
BpuAI 63 GAAGAC GAAGAC(2/6)
570 GAAGAC
BsaJI 42 CCTCGG C^C[ACGT][ACGT]GG
275 CCATGG
BsmFI 218 GGGAC GGGAC(10/14)
228 GGGAC
BsoBI 43 CTCGGG C^[CT]CG[GA]G
443 CTCGGG
BstF5I 338 GGATG GGATG(2/0)
618 GGATG
Cac8I 351 GCAAGC GC[ACGT]^[ACGT]GC
438 GCAAGC
Cfr13I 39 GGCCC G^G[ACGT]CC
169 GGACC
FokI 338 GGATG GGATG(9/13)
618 GGATG
HinfI 49 GATTC G^A[ACGT]TC
246 GAGTC
HphI 251 TCACC GGTGA(8/7)
302 TCACC
NlaIII 276 CATG CATG^
543 CATG
PshBI 288 ATTAAT AT^TAAT
387 ATTAAT
Sau96I 39 GGCCC G^G[ACGT]CC
169 GGACC
SfcI 119 CTACAG C^T[GA][CT]AG
518 CTGTAG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AcsI AluI ApoI BsmAI BsrI
DpnII MboI MboII MnlI MseI
NdeII Sau3AI Tru9I Tsp509I TspRI