(Circular) (Six-base) MAPSORT of: pFN21AE3641.seq Check: 1616 from: 1 to: 6214 pFN21AE3641 6214 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2536 2536 Size: 6214 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4024 278 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3557 1937 Size: 1620 4594 Acc65I G'GTAC_C Cuts at: 3535 3535 Size: 6214 AclI AA'CG_TT Cuts at: 1563 4341 4714 1563 Size: 2778 373 3063 Fragments arranged by size: 3063 2778 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2033 4277 5661 5929 1632 Size: 401 2244 1384 268 1917 Fragments arranged by size: 2244 1917 1384 401 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6017 AflIII A'CryG_T Cuts at: 1203 5113 6090 1203 Size: 3910 977 1327 Fragments arranged by size: 3910 1327 977 AgeI A'CCGG_T Cuts at: 2039 5045 2039 Size: 3006 3208 AhdI GACnn_n'nnGTC Cuts at: 2940 3253 4943 2940 Size: 313 1690 4211 Fragments arranged by size: 4211 1690 313 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6214 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6214 AlwNI CAG_nnn'CTG Cuts at: 2455 2487 2560 2641 2701 2722 2782 2905 2932 Size: 32 73 81 60 21 60 123 27 Cuts at: 2932 3123 3467 3957 5529 2455 Size: 191 344 490 1572 3140 Fragments arranged by size: 3140 1572 490 344 191 123 81 73 60 60 32 27 21 ApaI G_GGCC'C Cuts at: 2083 2097 2131 3242 2083 Size: 14 34 1111 5055 Fragments arranged by size: 5055 1111 34 14 ApaLI G'TGCA_C Cuts at: 2549 2830 4271 5427 2549 Size: 281 1441 1156 3336 Fragments arranged by size: 3336 1441 1156 281 ApoI r'AATT_y Cuts at: 1493 2767 3152 3523 3749 3901 6010 1493 Size: 1274 385 371 226 152 2109 1697 Fragments arranged by size: 2109 1697 1274 385 371 226 152 AseI AT'TA_AT Cuts at: 160 1032 4768 160 Size: 872 3736 1606 Fragments arranged by size: 3736 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 AvaI C'yCGr_G Cuts at: 1870 1943 2241 3326 3539 1870 Size: 73 298 1085 213 4545 Fragments arranged by size: 4545 1085 298 213 73 BamHI G'GATC_C Cuts at: 3505 3544 3911 3505 Size: 39 367 5808 Fragments arranged by size: 5808 367 39 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2254 2518 3306 3535 4990 Size: 325 217 627 467 264 788 229 1455 Cuts at: 4990 618 Size: 1842 Fragments arranged by size: 1842 1455 788 627 467 325 264 229 217 BanII G_rGCy'C Cuts at: 1391 2083 2097 2131 2478 3242 3394 3533 1391 Size: 692 14 34 347 764 152 139 4072 Fragments arranged by size: 4072 764 692 347 152 139 34 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2216 928 Size: 971 317 4926 Fragments arranged by size: 4926 971 317 BbvCI CC'TCA_GC Cuts at: 2391 2564 2391 Size: 173 6041 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2824 2858 4405 4439 1903 Size: 34 887 34 1547 34 3678 Fragments arranged by size: 3678 1547 887 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4106 5316 4106 Size: 1210 5004 BclI T'GATC_A Cuts at: 1548 1548 Size: 6214 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2333 2446 2501 Size: 107 122 71 631 605 661 113 55 Cuts at: 2501 4825 136 Size: 2324 1525 Fragments arranged by size: 2324 1525 661 631 605 122 113 107 71 55 BglII A'GATC_T Cuts at: 6209 6209 Size: 6214 BlpI GC'TnA_GC Cuts at: 3631 3631 Size: 6214 Bme1580I G_kGCm'C Cuts at: 1790 2083 2097 2131 2191 2523 2553 2764 2834 Size: 293 14 34 60 332 30 211 70 Cuts at: 2834 3242 3382 4275 5431 1790 Size: 408 140 893 1156 2573 Fragments arranged by size: 2573 1156 893 408 332 293 211 140 70 60 34 30 14 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6214 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2998 3448 3945 Size: 416 53 83 399 24 1581 450 497 Cuts at: 3945 4903 442 Size: 958 1753 Fragments arranged by size: 1753 1581 958 497 450 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2883 2998 4874 1239 Size: 1644 115 1876 2579 Fragments arranged by size: 2579 1876 1644 115 Bpu10I CC'TnA_GC Cuts at: 2391 2564 2894 2391 Size: 173 330 5711 Fragments arranged by size: 5711 330 173 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2639 4339 5204 5502 2639 Size: 1700 865 298 3351 Fragments arranged by size: 3351 1700 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3326 4877 882 Size: 617 1827 1551 2219 Fragments arranged by size: 2219 1827 1551 617 BsaAI yAC'GTr Cuts at: 493 1204 3196 4005 493 Size: 711 1992 809 2702 Fragments arranged by size: 2702 1992 809 711 BsaBI GATnn'nnATC Cuts at: 6208 6208 Size: 6214 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3189 4021 4403 5038 Size: 53 83 186 936 1656 832 382 635 Cuts at: 5038 275 Size: 1451 Fragments arranged by size: 1656 1451 936 832 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2039 4647 5045 5319 5466 5877 2039 Size: 2608 398 274 147 411 2376 Fragments arranged by size: 2608 2376 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5989 Fragments arranged by size: 5989 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 2486 2528 3344 1186 Size: 1000 300 42 816 4056 Fragments arranged by size: 4056 1000 816 300 42 BseYI C'CCAG_C Cuts at: 2050 2103 2223 2809 2845 5417 5913 2050 Size: 53 120 586 36 2572 496 2351 Fragments arranged by size: 2572 2351 586 496 120 53 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3329 1163 Size: 573 1593 4048 Fragments arranged by size: 4048 1593 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3664 4425 5453 1540 Size: 275 186 1663 761 1028 2301 Fragments arranged by size: 2301 1663 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2478 2553 2834 4275 4360 5431 2478 Size: 75 281 1441 85 1071 3261 Fragments arranged by size: 3261 1441 1071 281 85 75 BsmI GAATG_Cn' Cuts at: 2619 3179 3725 3818 2619 Size: 560 546 93 5015 Fragments arranged by size: 5015 560 546 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2097 2131 2191 2478 2523 2553 Size: 399 293 14 34 60 287 45 30 Cuts at: 2553 2764 2834 3242 3382 3394 3533 4275 4360 Size: 211 70 408 140 12 139 742 85 Cuts at: 4360 5431 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 408 399 293 287 211 140 139 85 70 60 45 34 30 14 12 BspEI T'CCGG_A Cuts at: 5877 5877 Size: 6214 BspHI T'CATG_A Cuts at: 4103 5833 4103 Size: 1730 4484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2536 2649 2913 3569 844 Size: 1692 113 264 656 3489 Fragments arranged by size: 3489 1692 656 264 113 BsrBI CCG'CTC Cuts at: 1394 1823 2238 2413 4101 1394 Size: 429 415 175 1688 3507 Fragments arranged by size: 3507 1688 429 415 175 BsrDI GCAATG_nn' Cuts at: 65 1248 4709 4883 65 Size: 1183 3461 174 1396 Fragments arranged by size: 3461 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2039 3309 3470 4858 5045 1700 Size: 110 229 1270 161 1388 187 2869 Fragments arranged by size: 2869 1388 1270 229 187 161 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6214 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6214 BssSI C'ACGA_G Cuts at: 4274 5286 4274 Size: 1012 5202 BstAPI GCAn_nnn'nTGC Cuts at: 794 2701 2710 3467 794 Size: 1907 9 757 3541 Fragments arranged by size: 3541 1907 757 9 BstBI TT'CG_AA Cuts at: 5018 5018 Size: 6214 BstEII G'GTnAC_C Cuts at: 1184 3025 1184 Size: 1841 4373 BstXI CCAn_nnnn'nTGG Cuts at: 2063 2063 Size: 6214 BstYI r'GATC_y Cuts at: 1972 2592 3505 3544 3911 4296 4313 5765 5851 Size: 620 913 39 367 385 17 1452 86 Cuts at: 5851 5863 6209 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 913 620 385 367 346 86 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 2676 2814 513 Size: 552 1611 138 3913 Fragments arranged by size: 3913 1611 552 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2643 3382 487 Size: 683 56 1417 739 3319 Fragments arranged by size: 3319 1417 739 683 56 BtsI GCAGTG_nn' Cuts at: 3210 3465 3730 4524 4544 3210 Size: 255 265 794 20 4880 Fragments arranged by size: 4880 794 265 255 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6214 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6179 DraI TTT'AAA Cuts at: 3518 3518 Size: 6214 DraIII CAC_nnn'GTG Cuts at: 3025 3025 Size: 6214 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5221 809 Size: 507 3905 1802 Fragments arranged by size: 3905 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2280 2325 2583 Size: 54 1425 50 275 18 450 45 258 Cuts at: 2583 3661 4550 6179 8 Size: 1078 889 1629 43 Fragments arranged by size: 1629 1425 1078 889 450 275 258 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3661 1537 Size: 275 1849 4090 Fragments arranged by size: 4090 1849 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2463 3419 4144 1353 Size: 1110 956 725 3423 Fragments arranged by size: 3423 1110 956 725 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2408 4801 5175 5321 387 Size: 1064 138 819 2393 374 146 1280 Fragments arranged by size: 2393 1280 1064 819 374 146 138 EcoICRI GAG'CTC Cuts at: 2476 2476 Size: 6214 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2883 2998 4277 4874 5661 5929 Size: 393 401 850 115 1279 597 787 268 Cuts at: 5929 1239 Size: 1524 Fragments arranged by size: 1524 1279 850 787 597 401 393 268 115 EcoNI CCTnn'n_nnAGG Cuts at: 2157 2168 2897 3416 2157 Size: 11 729 519 4955 Fragments arranged by size: 4955 729 519 11 EcoO109I rG'GnC_Cy Cuts at: 2023 2079 2080 2087 2094 2557 2806 2869 2890 Size: 56 1 7 7 463 249 63 21 Cuts at: 2890 2902 3145 3238 3239 3283 3409 2023 Size: 12 243 93 1 44 126 4828 Fragments arranged by size: 4828 463 249 243 126 93 63 56 44 21 12 7 7 1 1 EcoRI G'AATT_C Cuts at: 3523 3901 3523 Size: 378 5836 FspI TGC'GCA Cuts at: 4720 4720 Size: 6214 HaeII r_GCGC'y Cuts at: 1678 2430 5361 1678 Size: 752 2931 2531 Fragments arranged by size: 2931 2531 752 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2926 2958 3111 3143 3239 Size: 32 135 32 1354 32 153 32 96 Cuts at: 3239 3271 3381 3413 4855 4887 4929 4961 1373 Size: 32 110 32 1442 32 42 32 2626 Fragments arranged by size: 2626 1442 1354 153 135 110 96 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3558 3804 669 Size: 988 281 1620 246 3079 Fragments arranged by size: 3079 1620 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6214 HpaI GTT'AAC Cuts at: 3804 3804 Size: 6214 KpnI G_GTAC'C Cuts at: 3539 3539 Size: 6214 MfeI C'AATT_G Cuts at: 2538 3813 2538 Size: 1275 4939 MluI A'CGCG_T Cuts at: 6090 6090 Size: 6214 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3210 5328 5512 1305 Size: 1905 2118 184 2007 Fragments arranged by size: 2118 2007 1905 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2282 10 Size: 54 1425 343 450 3942 Fragments arranged by size: 3942 1425 450 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3089 3293 4172 4531 4690 518 Size: 648 1923 204 879 359 159 2042 Fragments arranged by size: 2042 1923 879 648 359 204 159 MspA1I CmG'CkG Cuts at: 1606 2050 2103 2227 2395 2445 2531 2678 2779 Size: 444 53 124 168 50 86 147 101 Cuts at: 2779 3107 3630 4307 5455 5700 1606 Size: 328 523 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 523 444 328 245 168 147 124 101 86 53 50 NaeI GCC'GGC Cuts at: 3472 3472 Size: 6214 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5662 NdeI CA'TA_TG Cuts at: 387 387 Size: 6214 NgoMIV G'CCGG_C Cuts at: 3470 3470 Size: 6214 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6214 NotI GC'GGCC_GC Cuts at: 3661 3661 Size: 6214 NspI r_CATG'y Cuts at: 1132 1229 3572 5117 1132 Size: 97 2343 1545 2229 Fragments arranged by size: 2343 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2083 3235 1348 Size: 735 1152 4327 Fragments arranged by size: 4327 1152 735 PciI A'CATG_T Cuts at: 5113 5113 Size: 6214 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6214 PfoI T'CCnGG_A Cuts at: 3417 3417 Size: 6214 PmeI GTTT'AAAC Cuts at: 3518 3518 Size: 6214 PmlI CAC'GTG Cuts at: 3196 3196 Size: 6214 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4283 4315 5796 5828 4283 Size: 32 1481 32 4669 Fragments arranged by size: 4669 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2806 2869 2902 3145 3283 3409 2806 Size: 63 33 243 138 126 5611 Fragments arranged by size: 5611 243 138 126 63 33 PshAI GACnn'nnGTC Cuts at: 1613 2742 1613 Size: 1129 5085 PsiI TTA'TAA Cuts at: 3784 3784 Size: 6214 PspOMI G'GGCC_C Cuts at: 2079 2093 2127 3238 2079 Size: 14 34 1111 5055 Fragments arranged by size: 5055 1111 34 14 PstI C_TGCA'G Cuts at: 830 2367 2400 2706 3106 3135 3407 3566 830 Size: 1537 33 306 400 29 272 159 3478 Fragments arranged by size: 3478 1537 400 306 272 159 33 29 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6214 PvuII CAG'CTG Cuts at: 2050 2227 2395 2779 3107 2050 Size: 177 168 384 328 5157 Fragments arranged by size: 5157 384 328 177 168 SacI G_AGCT'C Cuts at: 2478 2478 Size: 6214 SacII CC_GC'GG Cuts at: 2679 2679 Size: 6214 SalI G'TCGA_C Cuts at: 1936 3556 1936 Size: 1620 4594 SanDI GG'GwC_CC Cuts at: 2806 2869 2806 Size: 63 6151 SbfI CC_TGCA'GG Cuts at: 2706 3407 3566 2706 Size: 701 159 5354 Fragments arranged by size: 5354 701 159 ScaI AGT'ACT Cuts at: 2322 4462 6174 2322 Size: 2140 1712 2362 Fragments arranged by size: 2362 2140 1712 SexAI A'CCwGG_T Cuts at: 3021 3021 Size: 6214 SfcI C'TryA_G Cuts at: 826 1045 2363 2396 2702 3102 3131 3403 3562 Size: 219 1318 33 306 400 29 272 159 Cuts at: 3562 4697 5378 5569 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1318 1135 681 400 306 272 219 191 159 33 29 SfiI GGCCn_nnn'nGGCC Cuts at: 2501 2501 Size: 6214 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6214 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6214 SmaI CCC'GGG Cuts at: 1872 3541 1872 Size: 1669 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2241 2654 4318 5219 5481 820 Size: 197 926 298 413 1664 901 262 1553 Fragments arranged by size: 1664 1553 926 901 413 298 262 197 SnaBI TAC'GTA Cuts at: 493 4005 493 Size: 3512 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6214 SphI G_CATG'C Cuts at: 1132 3572 1132 Size: 2440 3774 SspI AAT'ATT Cuts at: 5 52 4138 5 Size: 47 4086 2081 Fragments arranged by size: 4086 2081 47 StuI AGG'CCT Cuts at: 1517 2163 2708 1517 Size: 646 545 5023 Fragments arranged by size: 5023 646 545 StyI C'CwwG_G Cuts at: 513 1065 2496 2508 3174 3653 513 Size: 552 1431 12 666 479 3074 Fragments arranged by size: 3074 1431 666 552 479 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2156 3248 3490 4241 4258 4411 4596 Size: 1255 576 1092 242 751 17 153 185 Cuts at: 4596 5041 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1092 751 576 445 242 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2320 4460 6172 Size: 274 80 33 51 1443 343 2140 1712 Cuts at: 6172 96 Size: 138 Fragments arranged by size: 2140 1712 1443 343 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2286 2318 6174 6206 2286 Size: 32 3856 32 2294 Fragments arranged by size: 3856 2294 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3125 1316 Size: 1809 4405 XbaI T'CTAG_A Cuts at: 3550 3550 Size: 6214 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2150 2150 Size: 6214 XhoI C'TCGA_G Cuts at: 1943 2241 1943 Size: 298 5916 XmaI C'CCGG_G Cuts at: 1870 3539 1870 Size: 1669 4545 XmnI GAAnn'nnTTC Cuts at: 4343 4343 Size: 6214 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4022 276 Size: 53 83 186 936 2488 2468 Fragments arranged by size: 2488 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SapI SfoI SrfI SwaI