################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BanI BbeI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BpmI BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI HapII HgaI Hin1I HincII HindII HpaI HpaII HphI KasI KpnI KspI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcsI 193 GAATTT [GA]^AATT[CT] AlwNI 643 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 263 AGCGCT AGC^GCT ApoI 193 GAATTT [GA]^AATT[CT] AspHI 583 GAGCAC G[AT]GC[AT]^C AvaII 758 GGTCC G^G[AT]CC BalI 729 TGGCCA TGG^CCA BamHI 162 GGATCC G^GATCC BbrPI 441 CACGTG CAC^GTG BfaI 737 CTAG C^TAG BlpI 30 GCTCAGC GC^T[ACGT]AGC Bpu1102I 30 GCTCAGC GC^T[ACGT]AGC BsaAI 441 CACGTG [CT]AC^GT[GA] BsgI 42 CTGCAC GTGCAG(16/14) BsiHKAI 583 GAGCAC G[AT]GC[AT]^C BsmAI 509 GAGAC GTCTC(1/5) BsrDI 272 GCAATG GCAATG(2/0) BssSI 378 CACGAG CACGAG(-5/-1) BstAPI 261 GCAGCGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 498 CCAGCCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 30 GCTCAGC GC^T[ACGT]AGC EarI 749 CTCTTC CTCTTC(1/4) Eco47III 263 AGCGCT AGC^GCT HaeII 263 AGCGCT [GA]GCGC^[CT] HindIII 460 AAGCTT A^AGCTT Ksp632I 749 CTCTTC CTCTTC(1/4) MaeI 737 CTAG C^TAG MaeII 442 ACGT A^CGT MluNI 729 TGGCCA TGG^CCA MscI 729 TGGCCA TGG^CCA PmaCI 441 CACGTG CAC^GTG PmlI 441 CACGTG CAC^GTG PstI 665 CTGCAG CTGCA^G PvuII 714 CAGCTG CAG^CTG SfcI 665 CTGCAG C^T[GA][CT]AG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 16 CCGC CCGC(-3/-1) 720 GCGG AviII 269 TGCGCA TGC^GCA 483 TGCGCA BclI 2 TGATCA T^GATCA 710 TGATCA BseRI 746 CTCCTC GAGGAG(10/8) 169 GAGGAG BspMI 154 ACCTGC ACCTGC(4/8) 52 GCAGGT BsrI 512 ACTGG ACTGG(1/-1) 617 ACTGG BstYI 144 GGATCT [GA]^GATC[CT] 162 GGATCC DdeI 31 CTCAG C^T[ACGT]AG 599 CTGAG EaeI 337 CGGCCA [CT]^GGCC[GA] 729 TGGCCA FbaI 2 TGATCA T^GATCA 710 TGATCA FspI 269 TGCGCA TGC^GCA 483 TGCGCA HinfI 105 GACTC G^A[ACGT]TC 672 GACTC MflI 144 GGATCT [GA]^GATC[CT] 162 GGATCC MseI 374 TTAA T^TAA 544 TTAA MspA1I 16 CCGCTG C[AC]G^C[GT]G 714 CAGCTG PleI 105 GACTC GAGTC(4/5) 672 GACTC Tru9I 374 TTAA T^TAA 544 TTAA Tsp45I 433 GTGAC ^GT[GC]AC 670 GTGAC XhoII 144 GGATCT [GA]^GATC[CT] 162 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AluI AlwI BanII BbvI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HhaI HinP1I ItaI MaeIII MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI Tsp509I TspRI