################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BglII BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AatII 967 GACGTC GACGT^C Alw44I 562 GTGCAC G^TGCAC ApaLI 562 GTGCAC G^TGCAC AvrII 1102 CCTAGG C^CTAGG BalI 1334 TGGCCA TGG^CCA BamHI 655 GGATCC G^GATCC BbsI 1057 GAAGAC GAAGAC(2/6) BcnI 1238 CCCGG CC^[GC]GG BfaI 1103 CTAG C^TAG BlnI 1102 CCTAGG C^CTAGG BpuAI 1057 GAAGAC GAAGAC(2/6) BsaI 798 GGTCTC GGTCTC(1/5) BsgI 1243 CTGCAC GTGCAG(16/14) BsmBI 693 CGTCTC CGTCTC(1/5) BspMI 611 ACCTGC ACCTGC(4/8) BsrBI 472 GAGCGG CCGCTC(-3/-3) BstXI 196 CCAGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 655 GGATCC [GA]^GATC[CT] EaeI 1334 TGGCCA [CT]^GGCC[GA] FauI 474 GCGGG CCCGC(4/6) HapII 1239 CCGG C^CGG HgaI 849 GCGTC GACGC(5/10) HpaII 1239 CCGG C^CGG MaeI 1103 CTAG C^TAG MflI 655 GGATCC [GA]^GATC[CT] MluNI 1334 TGGCCA TGG^CCA MscI 1334 TGGCCA TGG^CCA MslI 622 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1239 CCGG C^CGG NciI 1238 CCCGG CC^[GC]GG NspI 59 ACATGC [GA]CATG^[CT] PstI 704 CTGCAG CTGCA^G TfiI 245 GATTC G^A[AT]TC XhoII 655 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 288 CCGC CCGC(-3/-1) 474 GCGG AcsI 535 GAATTC [GA]^AATT[CT] 1144 GAATTC AcyI 848 GGCGTC G[GA]^CG[CT]C 967 GACGTC AfaI 171 GTAC GT^AC 778 GTAC ApaI 45 GGGCCC GGGCC^C 70 GGGCCC ApoI 535 GAATTC [GA]^AATT[CT] 1144 GAATTC AvaI 31 CTCGGG C^[CT]CG[GA]G 164 CTCGGG BclI 113 TGATCA T^GATCA 464 TGATCA BglI 305 GCCCTGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1327 GCCACTCTGGC BsaHI 848 GGCGTC G[GA]^CG[CT]C 967 GACGTC BsoBI 31 CTCGGG C^[CT]CG[GA]G 164 CTCGGG EarI 826 CTCTTC CTCTTC(1/4) 1174 GAAGAG EcoRI 535 GAATTC G^AATTC 1144 GAATTC FbaI 113 TGATCA T^GATCA 464 TGATCA Hin1I 848 GGCGTC G[GA]^CG[CT]C 967 GACGTC Ksp632I 826 CTCTTC CTCTTC(1/4) 1174 GAAGAG PflMI 86 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1132 CCACTGTCTGG RsaI 171 GTAC GT^AC 778 GTAC SacI 515 GAGCTC GAGCT^C 640 GAGCTC SfaNI 495 GCATC GCATC(5/9) 1207 GCATC Tsp509I 536 AATT ^AATT 1145 AATT Van91I 86 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1132 CCACTGTCTGG XcmI 326 CCAGTGACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 626 CCAGTGACCCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AluI AlwI AlwNI AocI AspHI AvaII BanII BbvI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Bsu36I Cac8I CelII Cfr13I DdeI DpnII DraII Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI PvuII Sau3AI Sau96I ScrFI SfcI StyI TseI Tsp45I TspRI