(Circular) (Six-base) MAPSORT of: pFN21AE4430.seq Check: 9235 from: 1 to: 6049 pFN21AE4430 6049 bp With 182 enzymes: SgfI * March 28, 2012 13:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2976 3859 278 Size: 53 83 186 936 1440 883 2468 Fragments arranged by size: 2468 1440 936 883 186 83 53 AccI GT'mk_AC Cuts at: 1937 3392 1937 Size: 1455 4594 Acc65I G'GTAC_C Cuts at: 3370 3370 Size: 6049 AclI AA'CG_TT Cuts at: 1563 4176 4549 1563 Size: 2613 373 3063 Fragments arranged by size: 3063 2613 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2575 3237 4112 5496 5764 1632 Size: 943 662 875 1384 268 1917 Fragments arranged by size: 1917 1384 943 875 662 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5852 AflIII A'CryG_T Cuts at: 1203 4948 5925 1203 Size: 3745 977 1327 Fragments arranged by size: 3745 1327 977 AgeI A'CCGG_T Cuts at: 4880 4880 Size: 6049 AhdI GACnn_n'nnGTC Cuts at: 4778 4778 Size: 6049 AleI CACnn'nnGTG Cuts at: 1166 2631 1166 Size: 1465 4584 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6049 AlwNI CAG_nnn'CTG Cuts at: 2114 2717 2915 2969 3792 5364 2114 Size: 603 198 54 823 1572 2799 Fragments arranged by size: 2799 1572 823 603 198 54 ApaI G_GGCC'C Cuts at: 2054 2079 2054 Size: 25 6024 ApaLI G'TGCA_C Cuts at: 2567 4106 5262 2567 Size: 1539 1156 3354 Fragments arranged by size: 3354 1539 1156 ApoI r'AATT_y Cuts at: 1493 2540 3149 3358 3584 3736 5845 1493 Size: 1047 609 209 226 152 2109 1697 Fragments arranged by size: 2109 1697 1047 609 226 209 152 AseI AT'TA_AT Cuts at: 160 1032 4603 160 Size: 872 3571 1606 Fragments arranged by size: 3571 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2169 3374 1870 Size: 73 93 133 1205 4545 Fragments arranged by size: 4545 1205 133 93 73 AvrII C'CTAG_G Cuts at: 3107 3107 Size: 6049 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2281 2314 2281 Size: 33 6016 BamHI G'GATC_C Cuts at: 2660 3379 3746 2660 Size: 719 367 4963 Fragments arranged by size: 4963 719 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3370 4825 618 Size: 325 217 627 1583 1455 1842 Fragments arranged by size: 1842 1583 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2054 2079 2235 2450 2524 2649 2931 3053 Size: 663 25 156 215 74 125 282 122 Cuts at: 3053 3321 3368 1391 Size: 268 47 4072 Fragments arranged by size: 4072 663 282 268 215 156 125 122 74 47 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3069 928 Size: 971 1170 3908 Fragments arranged by size: 3908 1170 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4240 4274 1903 Size: 34 2303 34 3678 Fragments arranged by size: 3678 2303 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3941 5151 3941 Size: 1210 4839 BclI T'GATC_A Cuts at: 1548 2118 2469 1548 Size: 570 351 5128 Fragments arranged by size: 5128 570 351 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2316 3338 4660 Size: 107 122 71 631 605 644 1022 1322 Cuts at: 4660 136 Size: 1525 Fragments arranged by size: 1525 1322 1022 644 631 605 122 107 71 BglII A'GATC_T Cuts at: 6044 6044 Size: 6049 BlpI GC'TnA_GC Cuts at: 2103 2523 3315 3466 2103 Size: 420 792 151 4686 Fragments arranged by size: 4686 792 420 151 Bme1580I G_kGCm'C Cuts at: 1790 2054 2079 2571 4110 5266 1790 Size: 264 25 492 1539 1156 2573 Fragments arranged by size: 2573 1539 1156 492 264 25 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6049 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2325 2625 2785 Size: 416 53 83 399 24 908 300 160 Cuts at: 2785 3315 3780 4738 442 Size: 530 465 958 1753 Fragments arranged by size: 1753 958 908 530 465 416 399 300 160 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6049 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2128 2128 Size: 6049 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 2249 2363 2663 2892 3085 3294 4709 Size: 944 66 114 300 229 193 209 1415 Cuts at: 4709 1239 Size: 2579 Fragments arranged by size: 2579 1415 944 300 229 209 193 114 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4174 5039 5337 4174 Size: 865 298 4886 Fragments arranged by size: 4886 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2809 4712 882 Size: 617 1310 1903 2219 Fragments arranged by size: 2219 1903 1310 617 BsaAI yAC'GTr Cuts at: 493 1204 3840 493 Size: 711 2636 2702 Fragments arranged by size: 2702 2636 711 BsaBI GATnn'nnATC Cuts at: 6043 6043 Size: 6049 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2854 2973 3856 4238 Size: 53 83 186 936 1321 119 883 382 Cuts at: 4238 4873 275 Size: 635 1451 Fragments arranged by size: 1451 1321 936 883 635 382 186 119 83 53 BsaWI w'CCGG_w Cuts at: 4482 4880 5154 5301 5712 4482 Size: 398 274 147 411 4819 Fragments arranged by size: 4819 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2591 2621 3268 3298 1178 Size: 30 165 30 1188 30 647 30 3929 Fragments arranged by size: 3929 1188 647 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2816 2819 2828 2831 2965 3117 3276 1186 Size: 1630 3 9 3 134 152 159 3959 Fragments arranged by size: 3959 1630 159 152 134 9 3 3 BseYI C'CCAG_C Cuts at: 2124 2200 2962 5252 5748 2124 Size: 76 762 2290 496 2425 Fragments arranged by size: 2425 2290 762 496 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3233 1163 Size: 573 1497 3979 Fragments arranged by size: 3979 1497 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3499 4260 5288 1540 Size: 275 186 1498 761 1028 2301 Fragments arranged by size: 2301 1498 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2517 2524 2571 2649 4110 4195 5266 2517 Size: 7 47 78 1461 85 1071 3300 Fragments arranged by size: 3300 1461 1071 85 78 47 7 BsmI GAATG_Cn' Cuts at: 3560 3653 3560 Size: 93 5956 BsmBI CGTCTCn'nnnn_ Cuts at: 2704 2704 Size: 6049 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2079 2235 2450 2517 2524 2571 Size: 399 264 25 156 215 67 7 47 Cuts at: 2571 2649 2931 3053 3321 3368 4110 4195 5266 Size: 78 282 122 268 47 742 85 1071 Cuts at: 5266 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 282 268 264 215 156 122 85 78 67 47 47 25 7 BspEI T'CCGG_A Cuts at: 5712 5712 Size: 6049 BspHI T'CATG_A Cuts at: 3938 5668 3938 Size: 1730 4319 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2625 3404 844 Size: 1781 779 3489 Fragments arranged by size: 3489 1781 779 BsrBI CCG'CTC Cuts at: 1394 1823 2479 3936 1394 Size: 429 656 1457 3507 Fragments arranged by size: 3507 1457 656 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4544 4718 65 Size: 1183 3296 174 1396 Fragments arranged by size: 3296 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4693 4880 1700 Size: 110 2883 187 2869 Fragments arranged by size: 2883 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6049 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6049 BssSI C'ACGA_G Cuts at: 4109 5121 4109 Size: 1012 5037 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6049 BstBI TT'CG_AA Cuts at: 4853 4853 Size: 6049 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6049 BstXI CCAn_nnnn'nTGG Cuts at: 2208 2208 Size: 6049 BstYI r'GATC_y Cuts at: 1972 2660 3379 3746 4131 4148 5600 5686 5698 Size: 688 719 367 385 17 1452 86 12 Cuts at: 5698 6044 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 719 688 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2410 2718 3018 2410 Size: 308 300 5441 Fragments arranged by size: 5441 308 300 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5497 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5310 Fragments arranged by size: 5310 683 56 BtsI GCAGTG_nn' Cuts at: 3081 3565 4359 4379 3081 Size: 484 794 20 4751 Fragments arranged by size: 4751 794 484 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6049 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6014 DraI TTT'AAA Cuts at: 3353 3353 Size: 6049 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5056 809 Size: 507 3740 1802 Fragments arranged by size: 3740 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3339 3496 4385 Size: 54 1425 50 275 18 1509 157 889 Cuts at: 4385 6014 8 Size: 1629 43 Fragments arranged by size: 1629 1509 1425 889 275 157 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3496 1537 Size: 275 1684 4090 Fragments arranged by size: 4090 1684 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2837 3174 3979 1353 Size: 1484 337 805 3423 Fragments arranged by size: 3423 1484 805 337 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4636 5010 5156 387 Size: 1064 138 3047 374 146 1280 Fragments arranged by size: 3047 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2522 2647 2522 Size: 125 5924 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2249 2363 2575 2663 2892 3085 Size: 393 551 66 114 212 88 229 193 Cuts at: 3085 3237 3294 4112 4709 5496 5764 1239 Size: 152 57 818 597 787 268 1524 Fragments arranged by size: 1524 818 787 597 551 393 268 229 212 193 152 114 88 66 57 EcoO109I rG'GnC_Cy Cuts at: 2051 2075 2076 2157 2577 2672 2679 2714 2941 Size: 24 1 81 420 95 7 35 227 Cuts at: 2941 3303 2051 Size: 362 4797 Fragments arranged by size: 4797 420 362 227 95 81 35 24 7 1 EcoRI G'AATT_C Cuts at: 2540 3149 3358 3736 2540 Size: 609 209 378 4853 Fragments arranged by size: 4853 609 378 209 FspI TGC'GCA Cuts at: 4555 4555 Size: 6049 HaeII r_GCGC'y Cuts at: 1678 5196 1678 Size: 3518 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2241 2273 4690 4722 4764 Size: 32 135 32 669 32 2417 32 42 Cuts at: 4764 4796 1373 Size: 32 2626 Fragments arranged by size: 2626 2417 669 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3393 3639 669 Size: 988 281 1455 246 3079 Fragments arranged by size: 3079 1455 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6049 HpaI GTT'AAC Cuts at: 3639 3639 Size: 6049 KpnI G_GTAC'C Cuts at: 3374 3374 Size: 6049 MfeI C'AATT_G Cuts at: 3648 3648 Size: 6049 MluI A'CGCG_T Cuts at: 5925 5925 Size: 6049 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2869 2923 5163 5347 1305 Size: 1564 54 2240 184 2007 Fragments arranged by size: 2240 2007 1564 184 54 MscI TGG'CCA Cuts at: 10 64 1489 1832 3341 10 Size: 54 1425 343 1509 2718 Fragments arranged by size: 2718 1509 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2631 4007 4366 4525 518 Size: 648 1465 1376 359 159 2042 Fragments arranged by size: 2042 1465 1376 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2124 2295 2708 2966 3314 3465 4142 5290 Size: 518 171 413 258 348 151 677 1148 Cuts at: 5290 5535 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 518 413 348 258 245 171 151 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5497 NdeI CA'TA_TG Cuts at: 387 387 Size: 6049 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6049 NotI GC'GGCC_GC Cuts at: 3496 3496 Size: 6049 NspI r_CATG'y Cuts at: 1132 1229 2068 3407 4952 1132 Size: 97 839 1339 1545 2229 Fragments arranged by size: 2229 1545 1339 839 97 PasI CC'CwG_GG Cuts at: 1348 2208 1348 Size: 860 5189 PciI A'CATG_T Cuts at: 4948 4948 Size: 6049 PflMI CCAn_nnn'nTGG Cuts at: 1108 2097 3143 1108 Size: 989 1046 4014 Fragments arranged by size: 4014 1046 989 PfoI T'CCnGG_A Cuts at: 3153 3153 Size: 6049 PmeI GTTT'AAAC Cuts at: 3353 3353 Size: 6049 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4118 4150 5631 5663 4118 Size: 32 1481 32 4504 Fragments arranged by size: 4504 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2157 2577 2672 2157 Size: 420 95 5534 Fragments arranged by size: 5534 420 95 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6049 PsiI TTA'TAA Cuts at: 3619 3619 Size: 6049 PspOMI G'GGCC_C Cuts at: 2050 2075 2050 Size: 25 6024 PstI C_TGCA'G Cuts at: 830 2713 3401 830 Size: 1883 688 3478 Fragments arranged by size: 3478 1883 688 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6049 PvuII CAG'CTG Cuts at: 2124 2708 2966 3314 2124 Size: 584 258 348 4859 Fragments arranged by size: 4859 584 348 258 SacI G_AGCT'C Cuts at: 2524 2649 2524 Size: 125 5924 SalI G'TCGA_C Cuts at: 1936 3391 1936 Size: 1455 4594 SanDI GG'GwC_CC Cuts at: 2157 2672 2157 Size: 515 5534 SbfI CC_TGCA'GG Cuts at: 3401 3401 Size: 6049 ScaI AGT'ACT Cuts at: 4297 6009 4297 Size: 1712 4337 SfcI C'TryA_G Cuts at: 826 1045 2313 2365 2581 2709 2739 2945 3397 Size: 219 1268 52 216 128 30 206 452 Cuts at: 3397 4532 5213 5404 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1268 1135 681 452 219 216 206 191 128 52 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6049 SmaI CCC'GGG Cuts at: 1872 3376 1872 Size: 1504 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4153 5054 5316 820 Size: 197 926 2210 901 262 1553 Fragments arranged by size: 2210 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3840 493 Size: 3347 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6049 SphI G_CATG'C Cuts at: 1132 3407 1132 Size: 2275 3774 SspI AAT'ATT Cuts at: 5 52 3973 5 Size: 47 3921 2081 Fragments arranged by size: 3921 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6049 StyI C'CwwG_G Cuts at: 513 1065 2664 2931 3087 3107 3488 513 Size: 552 1599 267 156 20 381 3074 Fragments arranged by size: 3074 1599 552 381 267 156 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2075 2701 4076 4093 4246 4431 4876 Size: 1255 495 626 1375 17 153 185 445 Cuts at: 4876 325 Size: 1498 Fragments arranged by size: 1498 1375 1255 626 495 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4295 6007 96 Size: 274 80 33 51 1443 2318 1712 138 Fragments arranged by size: 2318 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2406 2438 2591 2623 6009 6041 2406 Size: 32 153 32 3386 32 2414 Fragments arranged by size: 3386 2414 153 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6049 XbaI T'CTAG_A Cuts at: 3385 3385 Size: 6049 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2338 2638 2338 Size: 300 5749 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6049 XmaI C'CCGG_G Cuts at: 1870 3374 1870 Size: 1504 4545 XmnI GAAnn'nnTTC Cuts at: 4178 4178 Size: 6049 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2974 3857 276 Size: 53 83 186 936 1440 883 2468 Fragments arranged by size: 2468 1440 936 883 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI