(Linear) (Six-base) MAPSORT of: KIEE4430.seq Check: 6451 from: 1 to: 1344 KIEE4430.orf With 182 enzymes: SgfI * March 22, 2012 18:00 .. AatII G_ACGT'C Cuts at: 0 971 1344 Size: 971 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 570 1232 1344 Size: 570 662 112 Fragments arranged by size: 662 570 112 AleI CACnn'nnGTG Cuts at: 0 626 1344 Size: 626 718 AlwNI CAG_nnn'CTG Cuts at: 0 109 712 910 964 1344 Size: 109 603 198 54 380 Fragments arranged by size: 603 380 198 109 54 ApaI G_GGCC'C Cuts at: 0 49 74 1344 Size: 49 25 1270 Fragments arranged by size: 1270 49 25 ApaLI G'TGCA_C Cuts at: 0 562 1344 Size: 562 782 ApoI r'AATT_y Cuts at: 0 535 1144 1344 Size: 535 609 200 Fragments arranged by size: 609 535 200 AvaI C'yCGr_G Cuts at: 0 31 164 1344 Size: 31 133 1180 Fragments arranged by size: 1180 133 31 AvrII C'CTAG_G Cuts at: 0 1102 1344 Size: 1102 242 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 276 309 1344 Size: 276 33 1035 Fragments arranged by size: 1035 276 33 BamHI G'GATC_C Cuts at: 0 655 1344 Size: 655 689 BanII G_rGCy'C Cuts at: 0 49 74 230 445 519 644 926 1048 Size: 49 25 156 215 74 125 282 122 Cuts at: 1048 1316 1344 Size: 268 28 Fragments arranged by size: 282 268 215 156 125 122 74 49 28 25 BbsI GAAGACnn'nnnn_ Cuts at: 0 1064 1344 Size: 1064 280 BclI T'GATC_A Cuts at: 0 113 464 1344 Size: 113 351 880 Fragments arranged by size: 880 351 113 BglI GCCn_nnn'nGGC Cuts at: 0 311 1333 1344 Size: 311 1022 11 Fragments arranged by size: 1022 311 11 BlpI GC'TnA_GC Cuts at: 0 98 518 1310 1344 Size: 98 420 792 34 Fragments arranged by size: 792 420 98 34 Bme1580I G_kGCm'C Cuts at: 0 49 74 566 1344 Size: 49 25 492 778 Fragments arranged by size: 778 492 49 25 BmrI ACTGGGnnnn_n' Cuts at: 0 320 620 780 1310 1344 Size: 320 300 160 530 34 Fragments arranged by size: 530 320 300 160 34 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 123 1344 Size: 123 1221 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 244 358 658 887 1080 1289 1344 Size: 178 66 114 300 229 193 209 55 Fragments arranged by size: 300 229 209 193 178 114 66 55 BsaI GGTCTCn'nnnn_ Cuts at: 0 804 1344 Size: 804 540 BsaHI Gr'CG_yC Cuts at: 0 849 968 1344 Size: 849 119 376 Fragments arranged by size: 849 376 119 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 586 616 1263 1293 1344 Size: 586 30 647 30 51 Fragments arranged by size: 647 586 51 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 811 814 823 826 960 1112 1271 1344 Size: 811 3 9 3 134 152 159 73 Fragments arranged by size: 811 159 152 134 73 9 3 3 BseYI C'CCAG_C Cuts at: 0 119 195 957 1344 Size: 119 76 762 387 Fragments arranged by size: 762 387 119 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1228 1344 Size: 1228 116 BsiHKAI G_wGCw'C Cuts at: 0 512 519 566 644 1344 Size: 512 7 47 78 700 Fragments arranged by size: 700 512 78 47 7 BsmBI CGTCTCn'nnnn_ Cuts at: 0 699 1344 Size: 699 645 Bsp1286I G_dGCh'C Cuts at: 0 49 74 230 445 512 519 566 644 Size: 49 25 156 215 67 7 47 78 Cuts at: 644 926 1048 1316 1344 Size: 282 122 268 28 Fragments arranged by size: 282 268 215 156 122 78 67 49 47 28 25 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 620 1344 Size: 620 724 BsrBI CCG'CTC Cuts at: 0 474 1344 Size: 474 870 BstXI CCAn_nnnn'nTGG Cuts at: 0 203 1344 Size: 203 1141 BstYI r'GATC_y Cuts at: 0 655 1344 Size: 655 689 Bsu36I CC'TnA_GG Cuts at: 0 405 713 1013 1344 Size: 405 308 300 331 Fragments arranged by size: 405 331 308 300 BtsI GCAGTG_nn' Cuts at: 0 1076 1344 Size: 1076 268 EaeI y'GGCC_r Cuts at: 0 1334 1344 Size: 1334 10 EarI CTCTTCn'nnn_ Cuts at: 0 832 1169 1344 Size: 832 337 175 Fragments arranged by size: 832 337 175 EcoICRI GAG'CTC Cuts at: 0 517 642 1344 Size: 517 125 702 Fragments arranged by size: 702 517 125 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 244 358 570 658 887 1080 1232 Size: 178 66 114 212 88 229 193 152 Cuts at: 1232 1289 1344 Size: 57 55 Fragments arranged by size: 229 212 193 178 152 114 88 66 57 55 EcoO109I rG'GnC_Cy Cuts at: 0 46 70 71 152 572 667 674 709 Size: 46 24 1 81 420 95 7 35 Cuts at: 709 936 1298 1344 Size: 227 362 46 Fragments arranged by size: 420 362 227 95 81 46 46 35 24 7 1 EcoRI G'AATT_C Cuts at: 0 535 1144 1344 Size: 535 609 200 Fragments arranged by size: 609 535 200 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 236 268 1344 Size: 236 32 1076 Fragments arranged by size: 1076 236 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 864 918 1344 Size: 864 54 426 Fragments arranged by size: 864 426 54 MscI TGG'CCA Cuts at: 0 1336 1344 Size: 1336 8 MslI CAynn'nnrTG Cuts at: 0 626 1344 Size: 626 718 MspA1I CmG'CkG Cuts at: 0 119 290 703 961 1309 1344 Size: 119 171 413 258 348 35 Fragments arranged by size: 413 348 258 171 119 35 NspI r_CATG'y Cuts at: 0 63 1344 Size: 63 1281 PasI CC'CwG_GG Cuts at: 0 203 1344 Size: 203 1141 PflMI CCAn_nnn'nTGG Cuts at: 0 92 1138 1344 Size: 92 1046 206 Fragments arranged by size: 1046 206 92 PfoI T'CCnGG_A Cuts at: 0 1148 1344 Size: 1148 196 PpuMI rG'GwC_Cy Cuts at: 0 152 572 667 1344 Size: 152 420 95 677 Fragments arranged by size: 677 420 152 95 PspOMI G'GGCC_C Cuts at: 0 45 70 1344 Size: 45 25 1274 Fragments arranged by size: 1274 45 25 PstI C_TGCA'G Cuts at: 0 708 1344 Size: 708 636 PvuII CAG'CTG Cuts at: 0 119 703 961 1309 1344 Size: 119 584 258 348 35 Fragments arranged by size: 584 348 258 119 35 SacI G_AGCT'C Cuts at: 0 519 644 1344 Size: 519 125 700 Fragments arranged by size: 700 519 125 SanDI GG'GwC_CC Cuts at: 0 152 667 1344 Size: 152 515 677 Fragments arranged by size: 677 515 152 SfcI C'TryA_G Cuts at: 0 308 360 576 704 734 940 1344 Size: 308 52 216 128 30 206 404 Fragments arranged by size: 404 308 216 206 128 52 30 StyI C'CwwG_G Cuts at: 0 659 926 1082 1102 1344 Size: 659 267 156 20 242 Fragments arranged by size: 659 267 242 156 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 70 696 1344 Size: 70 626 648 Fragments arranged by size: 648 626 70 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 401 433 586 618 1344 Size: 401 32 153 32 726 Fragments arranged by size: 726 401 153 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 333 633 1344 Size: 333 300 711 Fragments arranged by size: 711 333 300 ZraI GAC'GTC Cuts at: 0 969 1344 Size: 969 375 Enzymes that do cut: AatII AcuI AleI AlwNI ApaI ApaLI ApoI AvaI AvrII BaeI BamHI BanII BbsI BclI BglI BlpI Bme1580I BmrI BplI BpmI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BstXI BstYI Bsu36I BtsI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I MmeI MscI MslI MspA1I NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI SfcI StyI TaqII TstI XcmI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AscI AseI AsiSI BanI BbeI BbvCI BcgI BciVI BfrBI BglII BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI XmaI XmnI