(Circular) (Six-base) MAPSORT of: pFN21AE2622.seq Check: 9062 from: 1 to: 5752 pFN21AE2622 5752 bp With 182 enzymes: SgfI * May 9, 2016 12:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3562 278 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3095 1937 Size: 1158 4594 Acc65I G'GTAC_C Cuts at: 3073 3073 Size: 5752 AclI AA'CG_TT Cuts at: 1563 3879 4252 1563 Size: 2316 373 3063 Fragments arranged by size: 3063 2316 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2662 2721 3815 5199 5467 1632 Size: 1030 59 1094 1384 268 1917 Fragments arranged by size: 1917 1384 1094 1030 268 59 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5555 AflIII A'CryG_T Cuts at: 1203 4651 5628 1203 Size: 3448 977 1327 Fragments arranged by size: 3448 1327 977 AgeI A'CCGG_T Cuts at: 4583 4583 Size: 5752 AhdI GACnn_n'nnGTC Cuts at: 4481 4481 Size: 5752 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5752 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5752 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2873 2905 2873 Size: 32 5720 AlwNI CAG_nnn'CTG Cuts at: 2282 2921 2960 3495 5067 2282 Size: 639 39 535 1572 2967 Fragments arranged by size: 2967 1572 639 535 39 ApaI G_GGCC'C Cuts at: 2013 2013 Size: 5752 ApaLI G'TGCA_C Cuts at: 3809 4965 3809 Size: 1156 4596 ApoI r'AATT_y Cuts at: 1493 3061 3287 3439 5548 1493 Size: 1568 226 152 2109 1697 Fragments arranged by size: 2109 1697 1568 226 152 AseI AT'TA_AT Cuts at: 160 1032 4306 160 Size: 872 3274 1606 Fragments arranged by size: 3274 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 AvaI C'yCGr_G Cuts at: 1870 1943 3077 1870 Size: 73 1134 4545 Fragments arranged by size: 4545 1134 73 BamHI G'GATC_C Cuts at: 2357 2888 3082 3449 2357 Size: 531 194 367 4660 Fragments arranged by size: 4660 531 367 194 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3073 4528 618 Size: 325 217 627 1286 1455 1842 Fragments arranged by size: 1842 1455 1286 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2058 3071 1391 Size: 622 45 1013 4072 Fragments arranged by size: 4072 1013 622 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4781 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3943 3977 1903 Size: 34 2006 34 3678 Fragments arranged by size: 3678 2006 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3644 4854 3644 Size: 1210 4542 BclI T'GATC_A Cuts at: 1548 2949 1548 Size: 1401 4351 BfrBI ATG'CAT Cuts at: 2318 2318 Size: 5752 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3041 4363 136 Size: 107 122 71 631 605 1369 1322 1525 Fragments arranged by size: 1525 1369 1322 631 605 122 107 71 BglII A'GATC_T Cuts at: 5747 5747 Size: 5752 BlpI GC'TnA_GC Cuts at: 2106 3169 2106 Size: 1063 4689 Bme1580I G_kGCm'C Cuts at: 1790 2013 3813 4969 1790 Size: 223 1800 1156 2573 Fragments arranged by size: 2573 1800 1156 223 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5752 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2681 3483 4441 Size: 416 53 83 399 24 1264 802 958 Cuts at: 4441 442 Size: 1753 Fragments arranged by size: 1753 1264 958 802 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5752 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2437 4412 1239 Size: 1198 1975 2579 Fragments arranged by size: 2579 1975 1198 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3877 4742 5040 3877 Size: 865 298 4589 Fragments arranged by size: 4589 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4415 882 Size: 617 2916 2219 Fragments arranged by size: 2916 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3543 493 Size: 711 2339 2702 Fragments arranged by size: 2702 2339 711 BsaBI GATnn'nnATC Cuts at: 5746 5746 Size: 5752 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3559 3941 4576 275 Size: 53 83 186 936 2026 382 635 1451 Fragments arranged by size: 2026 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2428 4185 4583 4857 5004 5415 2428 Size: 1757 398 274 147 411 2765 Fragments arranged by size: 2765 1757 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5527 Fragments arranged by size: 5527 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5752 BseYI C'CCAG_C Cuts at: 2662 4955 5451 2662 Size: 2293 496 2963 Fragments arranged by size: 2963 2293 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5179 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3202 3963 4991 1540 Size: 275 186 1201 761 1028 2301 Fragments arranged by size: 2301 1201 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3813 3898 4969 3813 Size: 85 1071 4596 Fragments arranged by size: 4596 1071 85 BsmI GAATG_Cn' Cuts at: 3263 3356 3263 Size: 93 5659 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2058 3071 3813 3898 4969 1391 Size: 399 223 45 1013 742 85 1071 2174 Fragments arranged by size: 2174 1071 1013 742 399 223 85 45 BspEI T'CCGG_A Cuts at: 2428 5415 2428 Size: 2987 2765 BspHI T'CATG_A Cuts at: 3018 3641 5371 3018 Size: 623 1730 3399 Fragments arranged by size: 3399 1730 623 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3107 844 Size: 2263 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3639 1394 Size: 429 1816 3507 Fragments arranged by size: 3507 1816 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4247 4421 65 Size: 1183 2999 174 1396 Fragments arranged by size: 2999 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4396 4583 1700 Size: 110 2586 187 2869 Fragments arranged by size: 2869 2586 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5752 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5752 BssSI C'ACGA_G Cuts at: 2190 3812 4824 2190 Size: 1622 1012 3118 Fragments arranged by size: 3118 1622 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5752 BstBI TT'CG_AA Cuts at: 4556 4556 Size: 5752 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5752 BstXI CCAn_nnnn'nTGG Cuts at: 2473 2473 Size: 5752 BstYI r'GATC_y Cuts at: 1972 2357 2888 3082 3449 3834 3851 5303 5389 Size: 385 531 194 367 385 17 1452 86 Cuts at: 5389 5401 5747 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 531 385 385 367 346 194 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2472 513 Size: 552 939 468 3793 Fragments arranged by size: 3793 939 552 468 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5013 Fragments arranged by size: 5013 683 56 BtsI GCAGTG_nn' Cuts at: 2100 3268 4062 4082 2100 Size: 1168 794 20 3770 Fragments arranged by size: 3770 1168 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5752 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5717 DraI TTT'AAA Cuts at: 3056 3056 Size: 5752 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4759 809 Size: 507 3443 1802 Fragments arranged by size: 3443 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2410 2923 3033 Size: 54 1425 50 275 18 580 513 110 Cuts at: 3033 3199 4088 5717 8 Size: 166 889 1629 43 Fragments arranged by size: 1629 1425 889 580 513 275 166 110 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3199 1537 Size: 275 1387 4090 Fragments arranged by size: 4090 1387 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3682 1353 Size: 2329 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4339 4713 4859 387 Size: 1064 138 2750 374 146 1280 Fragments arranged by size: 2750 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2437 2662 2721 3815 4412 5199 5467 Size: 393 805 225 59 1094 597 787 268 Cuts at: 5467 1239 Size: 1524 Fragments arranged by size: 1524 1094 805 787 597 393 268 225 59 EcoO109I rG'GnC_Cy Cuts at: 2009 2185 2279 2598 2636 2009 Size: 176 94 319 38 5125 Fragments arranged by size: 5125 319 176 94 38 EcoRI G'AATT_C Cuts at: 3061 3439 3061 Size: 378 5374 FspI TGC'GCA Cuts at: 4258 4258 Size: 5752 HaeII r_GCGC'y Cuts at: 1678 4899 1678 Size: 3221 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2025 2057 2342 2374 2590 Size: 32 135 32 453 32 285 32 216 Cuts at: 2590 2622 2994 3026 4393 4425 4467 4499 1373 Size: 32 372 32 1367 32 42 32 2626 Fragments arranged by size: 2626 1367 453 372 285 216 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3096 3342 669 Size: 988 281 1158 246 3079 Fragments arranged by size: 3079 1158 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5752 HpaI GTT'AAC Cuts at: 3342 3342 Size: 5752 KpnI G_GTAC'C Cuts at: 3077 3077 Size: 5752 MfeI C'AATT_G Cuts at: 3351 3351 Size: 5752 MluI A'CGCG_T Cuts at: 5628 5628 Size: 5752 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2490 4866 5050 1305 Size: 1185 2376 184 2007 Fragments arranged by size: 2376 2007 1185 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2412 3035 10 Size: 54 1425 343 580 623 2727 Fragments arranged by size: 2727 1425 623 580 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3710 4069 4228 518 Size: 648 2544 359 159 2042 Fragments arranged by size: 2544 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2581 2886 3168 3845 4993 5238 1606 Size: 975 305 282 677 1148 245 2120 Fragments arranged by size: 2120 1148 975 677 305 282 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4261 Fragments arranged by size: 4261 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5752 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5752 NotI GC'GGCC_GC Cuts at: 3199 3199 Size: 5752 NsiI A_TGCA'T Cuts at: 2320 2320 Size: 5752 NspI r_CATG'y Cuts at: 1132 1229 3110 4655 1132 Size: 97 1881 1545 2229 Fragments arranged by size: 2229 1881 1545 97 PasI CC'CwG_GG Cuts at: 1348 2276 1348 Size: 928 4824 PciI A'CATG_T Cuts at: 4651 4651 Size: 5752 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5752 PmeI GTTT'AAAC Cuts at: 3056 3056 Size: 5752 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3821 3853 5334 5366 3821 Size: 32 1481 32 4207 Fragments arranged by size: 4207 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2185 2598 2636 2185 Size: 413 38 5301 Fragments arranged by size: 5301 413 38 PshAI GACnn'nnGTC Cuts at: 1613 2939 1613 Size: 1326 4426 PsiI TTA'TAA Cuts at: 3322 3322 Size: 5752 PspOMI G'GGCC_C Cuts at: 2009 2009 Size: 5752 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2513 2545 2513 Size: 32 5720 PstI C_TGCA'G Cuts at: 830 2033 2143 2580 3104 830 Size: 1203 110 437 524 3478 Fragments arranged by size: 3478 1203 524 437 110 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5752 PvuII CAG'CTG Cuts at: 2581 2581 Size: 5752 SalI G'TCGA_C Cuts at: 1936 3094 1936 Size: 1158 4594 SbfI CC_TGCA'GG Cuts at: 3104 3104 Size: 5752 ScaI AGT'ACT Cuts at: 3030 4000 5712 3030 Size: 970 1712 3070 Fragments arranged by size: 3070 1712 970 SfcI C'TryA_G Cuts at: 826 1045 2029 2139 2576 3100 4235 4916 5107 Size: 219 984 110 437 524 1135 681 191 Cuts at: 5107 826 Size: 1471 Fragments arranged by size: 1471 1135 984 681 524 437 219 191 110 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5752 SmaI CCC'GGG Cuts at: 1872 3079 1872 Size: 1207 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3856 4757 5019 820 Size: 197 926 1913 901 262 1553 Fragments arranged by size: 1913 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3543 493 Size: 3050 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5752 SphI G_CATG'C Cuts at: 1132 3110 1132 Size: 1978 3774 SspI AAT'ATT Cuts at: 5 52 3676 5 Size: 47 3624 2081 Fragments arranged by size: 3624 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5752 StyI C'CwwG_G Cuts at: 513 1065 2004 3191 513 Size: 552 939 1187 3074 Fragments arranged by size: 3074 1187 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3779 3796 3949 4134 4579 325 Size: 1255 2199 17 153 185 445 1498 Fragments arranged by size: 2199 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2971 3028 3998 Size: 274 80 33 51 1443 994 57 970 Cuts at: 3998 5710 96 Size: 1712 138 Fragments arranged by size: 1712 1443 994 970 274 138 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5712 5744 5712 Size: 32 5720 Tth111I GACn'n_nGTC Cuts at: 1316 2687 1316 Size: 1371 4381 XbaI T'CTAG_A Cuts at: 3088 3088 Size: 5752 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2189 2189 Size: 5752 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5752 XmaI C'CCGG_G Cuts at: 1870 3077 1870 Size: 1207 4545 XmnI GAAnn'nnTTC Cuts at: 3881 3881 Size: 5752 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3560 276 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI