################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmFI BsoBI BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI BstYI Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FseI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MscI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccIII 663 TCCGGA T^CCGGA AciI 912 CCGC CCGC(-3/-1) AlwI 1503 GGATC GGATC(4/5) AspHI 116 GAGCAC G[AT]GC[AT]^C AviII 833 TGCGCA TGC^GCA BbsI 1270 GAAGAC GAAGAC(2/6) BcgI 978 CGAAACTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 58 GTATCC GTATCC(6/5) BclI 927 TGATCA T^GATCA BmyI 116 GAGCAC G[AGT]GC[ACT]^C BpmI 1276 CTGGAG CTGGAG(16/14) BpuAI 1270 GAAGAC GAAGAC(2/6) BsaAI 1250 TACGTG [CT]AC^GT[GA] BsaI 450 GAGACC GGTCTC(1/5) BsaJI 1415 CCATGG C^C[ACGT][ACGT]GG BseAI 663 TCCGGA T^CCGGA BsiHKAI 116 GAGCAC G[AT]GC[AT]^C BsmI 159 GAATGC GAATGC(1/-1) Bsp1286I 116 GAGCAC G[AGT]GC[ACT]^C Bsp1407I 528 TGTACA T^GTACA BspEI 663 TCCGGA T^CCGGA BsrGI 528 TGTACA T^GTACA Bst1107I 966 GTATAC GTA^TAC BstF5I 782 CATCC GGATG(2/0) BstZ17I 966 GTATAC GTA^TAC Cac8I 1052 GCTTGC GC[ACGT]^[ACGT]GC CfoI 834 GCGC GCG^C Cfr13I 152 GGGCC G^G[ACGT]CC DsaI 1415 CCATGG C^C[GA][CT]GG EaeI 631 CGGCCA [CT]^GGCC[GA] EarI 214 CTCTTC CTCTTC(1/4) EcoRI 1176 GAATTC G^AATTC EcoT14I 1415 CCATGG C^C[AT][AT]GG FauI 911 CCCGC CCCGC(4/6) FbaI 927 TGATCA T^GATCA FokI 782 CATCC GGATG(9/13) FspI 833 TGCGCA TGC^GCA HhaI 834 GCGC GCG^C HinP1I 834 GCGC G^CGC HindIII 36 AAGCTT A^AGCTT Ksp632I 214 CTCTTC CTCTTC(1/4) MroI 663 TCCGGA T^CCGGA MseI 44 TTAA T^TAA NcoI 1415 CCATGG C^CATGG NlaIV 1018 GGAACC GG[ACGT]^[ACGT]CC PleI 102 GACTC GAGTC(4/5) Sau96I 152 GGGCC G^G[ACGT]CC SfcI 812 CTGTAG C^T[GA][CT]AG SspBI 528 TGTACA T^GTACA SspI 267 AATATT AAT^ATT StyI 1415 CCATGG C^C[AT][AT]GG TaqI 840 TCGA T^CGA Tru9I 44 TTAA T^TAA TthHB8I 840 TCGA T^CGA XcmI 634 CCAAATCACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AccI 409 GTAGAC GT^[AC][GT]AC 966 GTATAC AfaI 529 GTAC GT^AC 1249 GTAC Asp700I 508 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 588 GAAGGATTTC BcnI 251 CCGGG CC^[GC]GG 694 CCCGG BsaWI 663 TCCGGA [AT]^CCGG[AT] 894 TCCGGT MaeII 1103 ACGT A^CGT 1251 ACGT MspA1I 747 CAGCTG C[AC]G^C[GT]G 1305 CAGCTG NciI 251 CCGGG CC^[GC]GG 694 CCCGG PvuII 747 CAGCTG CAG^CTG 1305 CAGCTG RsaI 529 GTAC GT^AC 1249 GTAC Tsp45I 1105 GTCAC ^GT[GC]AC 1184 GTGAC XmnI 508 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 588 GAAGGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AcsI AluI ApoI BbvI BfaI BsmAI BsrI BssKI BstNI DdeI DpnII EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII Sau3AI ScrFI SfaNI SmlI TfiI TseI Tsp509I TspRI