(Circular) (Six-base) MAPSORT of: pFN21AE5612.seq Check: 9857 from: 1 to: 6238 pFN21AE5612 6238 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4048 278 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2415 2972 3581 1937 Size: 478 557 609 4594 Fragments arranged by size: 4594 609 557 478 Acc65I G'GTAC_C Cuts at: 3559 3559 Size: 6238 AclI AA'CG_TT Cuts at: 1563 4365 4738 1563 Size: 2802 373 3063 Fragments arranged by size: 3063 2802 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4301 5685 5953 1632 Size: 2669 1384 268 1917 Fragments arranged by size: 2669 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6041 AflIII A'CryG_T Cuts at: 1203 5137 6114 1203 Size: 3934 977 1327 Fragments arranged by size: 3934 1327 977 AgeI A'CCGG_T Cuts at: 5069 5069 Size: 6238 AhdI GACnn_n'nnGTC Cuts at: 4967 4967 Size: 6238 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6238 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6238 AlwNI CAG_nnn'CTG Cuts at: 3981 5553 3981 Size: 1572 4666 ApaLI G'TGCA_C Cuts at: 4295 5451 4295 Size: 1156 5082 ApoI r'AATT_y Cuts at: 1493 2018 2113 2177 2192 2576 2945 3181 3299 Size: 525 95 64 15 384 369 236 118 Cuts at: 3299 3441 3547 3773 3925 6034 1493 Size: 142 106 226 152 2109 1697 Fragments arranged by size: 2109 1697 525 384 369 236 226 152 142 118 106 95 64 15 AseI AT'TA_AT Cuts at: 160 1032 4792 160 Size: 872 3760 1606 Fragments arranged by size: 3760 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 AvaI C'yCGr_G Cuts at: 1870 1943 3563 1870 Size: 73 1620 4545 Fragments arranged by size: 4545 1620 73 BamHI G'GATC_C Cuts at: 3568 3935 3568 Size: 367 5871 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3559 5014 618 Size: 325 217 627 1772 1455 1842 Fragments arranged by size: 1842 1772 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3557 1391 Size: 2166 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3282 928 Size: 971 1383 3884 Fragments arranged by size: 3884 1383 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2972 3006 4429 4463 1903 Size: 34 1035 34 1423 34 3678 Fragments arranged by size: 3678 1423 1035 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2074 4130 5340 2074 Size: 2056 1210 2972 Fragments arranged by size: 2972 2056 1210 BclI T'GATC_A Cuts at: 1548 2932 1548 Size: 1384 4854 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4849 136 Size: 107 122 71 631 605 3177 1525 Fragments arranged by size: 3177 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6233 6233 Size: 6238 BlpI GC'TnA_GC Cuts at: 3655 3655 Size: 6238 Bme1580I G_kGCm'C Cuts at: 1790 4299 5455 1790 Size: 2509 1156 2573 Fragments arranged by size: 2573 2509 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6238 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2452 3969 4927 Size: 416 53 83 399 24 1035 1517 958 Cuts at: 4927 442 Size: 1753 Fragments arranged by size: 1753 1517 1035 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6238 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3302 4898 1239 Size: 2063 1596 2579 Fragments arranged by size: 2579 2063 1596 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2940 3251 3346 4363 5228 5526 2940 Size: 311 95 1017 865 298 3652 Fragments arranged by size: 3652 1017 865 311 298 95 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2449 4901 882 Size: 617 950 2452 2219 Fragments arranged by size: 2452 2219 950 617 BsaAI yAC'GTr Cuts at: 493 1204 3257 4029 493 Size: 711 2053 772 2702 Fragments arranged by size: 2702 2053 772 711 BsaBI GATnn'nnATC Cuts at: 6232 6232 Size: 6238 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4045 4427 5062 275 Size: 53 83 186 936 2512 382 635 1451 Fragments arranged by size: 2512 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2668 2899 4671 5069 5343 5490 5901 2668 Size: 231 1772 398 274 147 411 3005 Fragments arranged by size: 3005 1772 411 398 274 231 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2316 2346 1178 Size: 30 165 30 913 30 5070 Fragments arranged by size: 5070 913 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6238 BseYI C'CCAG_C Cuts at: 5441 5937 5441 Size: 496 5742 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5665 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3688 4449 5477 1540 Size: 275 186 1687 761 1028 2301 Fragments arranged by size: 2301 1687 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2125 4299 4384 5455 2125 Size: 2174 85 1071 2908 Fragments arranged by size: 2908 2174 1071 85 BsmI GAATG_Cn' Cuts at: 2170 3749 3842 2170 Size: 1579 93 4566 Fragments arranged by size: 4566 1579 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2125 3557 4299 4384 5455 1391 Size: 399 335 1432 742 85 1071 2174 Fragments arranged by size: 2174 1432 1071 742 399 335 85 BspEI T'CCGG_A Cuts at: 2668 5901 2668 Size: 3233 3005 BspHI T'CATG_A Cuts at: 4127 5857 4127 Size: 1730 4508 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3593 844 Size: 2749 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4125 1394 Size: 429 2302 3507 Fragments arranged by size: 3507 2302 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4733 4907 65 Size: 1183 3485 174 1396 Fragments arranged by size: 3485 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4882 5069 1700 Size: 110 3072 187 2869 Fragments arranged by size: 3072 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2533 96 Size: 2437 3801 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6238 BssSI C'ACGA_G Cuts at: 4298 5310 4298 Size: 1012 5226 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6238 BstBI TT'CG_AA Cuts at: 5042 5042 Size: 6238 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6238 BstYI r'GATC_y Cuts at: 1972 3568 3935 4320 4337 5789 5875 5887 6233 Size: 1596 367 385 17 1452 86 12 346 Cuts at: 6233 1972 Size: 1977 Fragments arranged by size: 1977 1596 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2973 2973 Size: 6238 BtgI C'CryG_G Cuts at: 513 1065 2004 3420 513 Size: 552 939 1416 3331 Fragments arranged by size: 3331 1416 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5499 Fragments arranged by size: 5499 683 56 BtsI GCAGTG_nn' Cuts at: 2772 3754 4548 4568 2772 Size: 982 794 20 4442 Fragments arranged by size: 4442 982 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6238 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6203 DraI TTT'AAA Cuts at: 3542 3542 Size: 6238 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5245 809 Size: 507 3929 1802 Fragments arranged by size: 3929 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2636 3685 4574 Size: 54 1425 50 275 18 806 1049 889 Cuts at: 4574 6203 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1049 889 806 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3685 1537 Size: 275 1873 4090 Fragments arranged by size: 4090 1873 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2225 4168 1353 Size: 872 1943 3423 Fragments arranged by size: 3423 1943 872 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4825 5199 5345 387 Size: 1064 138 3236 374 146 1280 Fragments arranged by size: 3236 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3302 4301 4898 5685 5953 1239 Size: 393 1670 999 597 787 268 1524 Fragments arranged by size: 1670 1524 999 787 597 393 268 EcoRI G'AATT_C Cuts at: 3181 3547 3925 3181 Size: 366 378 5494 Fragments arranged by size: 5494 378 366 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2047 2377 2981 2047 Size: 330 604 5304 Fragments arranged by size: 5304 604 330 FspI TGC'GCA Cuts at: 2840 4744 2840 Size: 1904 4334 HaeII r_GCGC'y Cuts at: 1678 5385 1678 Size: 3707 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4879 4911 4953 4985 1373 Size: 32 135 32 3307 32 42 32 2626 Fragments arranged by size: 3307 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3582 3828 669 Size: 988 281 1644 246 3079 Fragments arranged by size: 3079 1644 988 281 246 HindIII A'AGCT_T Cuts at: 748 2041 748 Size: 1293 4945 HpaI GTT'AAC Cuts at: 3828 3828 Size: 6238 KpnI G_GTAC'C Cuts at: 3563 3563 Size: 6238 MfeI C'AATT_G Cuts at: 3837 3837 Size: 6238 MluI A'CGCG_T Cuts at: 6114 6114 Size: 6238 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5352 5536 1305 Size: 4047 184 2007 Fragments arranged by size: 4047 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4416 Fragments arranged by size: 4416 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4196 4555 4714 518 Size: 648 843 2187 359 159 2042 Fragments arranged by size: 2187 2042 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2754 3312 3654 4331 5479 5724 1606 Size: 1148 558 342 677 1148 245 2120 Fragments arranged by size: 2120 1148 1148 677 558 342 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3420 513 Size: 552 939 1416 3331 Fragments arranged by size: 3331 1416 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6238 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6238 NotI GC'GGCC_GC Cuts at: 3685 3685 Size: 6238 NspI r_CATG'y Cuts at: 1132 1229 3596 5141 1132 Size: 97 2367 1545 2229 Fragments arranged by size: 2367 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6238 PciI A'CATG_T Cuts at: 5137 5137 Size: 6238 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6238 PmeI GTTT'AAAC Cuts at: 3542 3542 Size: 6238 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4307 4339 5820 5852 4307 Size: 32 1481 32 4693 Fragments arranged by size: 4693 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6238 PsiI TTA'TAA Cuts at: 3808 3808 Size: 6238 PstI C_TGCA'G Cuts at: 830 3590 830 Size: 2760 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6238 PvuII CAG'CTG Cuts at: 2754 3312 2754 Size: 558 5680 SalI G'TCGA_C Cuts at: 1936 3580 1936 Size: 1644 4594 SbfI CC_TGCA'GG Cuts at: 3590 3590 Size: 6238 ScaI AGT'ACT Cuts at: 4486 6198 4486 Size: 1712 4526 SfcI C'TryA_G Cuts at: 826 1045 2817 3586 4721 5402 5593 826 Size: 219 1772 769 1135 681 191 1471 Fragments arranged by size: 1772 1471 1135 769 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6238 SmaI CCC'GGG Cuts at: 1872 3565 1872 Size: 1693 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2955 3230 3325 4342 5243 5505 Size: 197 926 1012 275 95 1017 901 262 Cuts at: 5505 820 Size: 1553 Fragments arranged by size: 1553 1017 1012 926 901 275 262 197 95 SnaBI TAC'GTA Cuts at: 493 4029 493 Size: 3536 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6238 SphI G_CATG'C Cuts at: 1132 3596 1132 Size: 2464 3774 SspI AAT'ATT Cuts at: 5 52 2274 4162 5 Size: 47 2222 1888 2081 Fragments arranged by size: 2222 2081 1888 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6238 StyI C'CwwG_G Cuts at: 513 1065 2004 3420 3677 513 Size: 552 939 1416 257 3074 Fragments arranged by size: 3074 1416 939 552 257 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2987 4265 4282 4435 4620 5065 325 Size: 1255 1407 1278 17 153 185 445 1498 Fragments arranged by size: 1498 1407 1278 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2533 4484 6196 Size: 274 80 33 51 1443 556 1951 1712 Cuts at: 6196 96 Size: 138 Fragments arranged by size: 1951 1712 1443 556 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2316 2348 3172 3204 6198 6230 2316 Size: 32 824 32 2994 32 2324 Fragments arranged by size: 2994 2324 824 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6238 XbaI T'CTAG_A Cuts at: 3574 3574 Size: 6238 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2646 2646 Size: 6238 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6238 XmaI C'CCGG_G Cuts at: 1870 3563 1870 Size: 1693 4545 XmnI GAAnn'nnTTC Cuts at: 2517 2597 4367 2517 Size: 80 1770 4388 Fragments arranged by size: 4388 1770 80 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4046 276 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI