################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BsaAI BseAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MfeI MluI MluNI MroI MscI MseI MunI MvnI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 54 GACGTC GACGT^C AgeI 278 ACCGGT A^CCGGT AlwI 480 GATCC GGATC(4/5) AlwNI 256 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 25 GTGCTC G[AT]GC[AT]^C BanII 195 GGGCTC G[GA]GC[CT]^C BbeI 115 GGCGCC GGCGC^C BbsI 51 GAAGAC GAAGAC(2/6) BglII 685 AGATCT A^GATCT BlpI 523 GCTGAGC GC^T[ACGT]AGC Bpu1102I 523 GCTGAGC GC^T[ACGT]AGC BpuAI 51 GAAGAC GAAGAC(2/6) BsaBI 241 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 278 ACCGGT [AT]^CCGG[AT] BseRI 355 GAGGAG GAGGAG(10/8) BsiHKAI 25 GTGCTC G[AT]GC[AT]^C BspMI 441 GCAGGT ACCTGC(4/8) BsrBI 79 GAGCGG CCGCTC(-3/-3) BsrI 659 CCAGT ACTGG(1/-1) CelII 523 GCTGAGC GC^T[ACGT]AGC DraII 361 GGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 643 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 186 CCACGG C^C[GA][CT]GG EcoNI 348 CCTCACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 361 GGGGCCT [GA]G^G[ACGT]CC[CT] HgaI 406 GACGC GACGC(5/10) HinfI 246 GAATC G^A[ACGT]TC KasI 115 GGCGCC G^GCGCC MaeIII 639 GTCAC ^GT[ACGT]AC MamI 241 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 39 CATGAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 516 GCCGGC GCC^GGC NarI 115 GGCGCC GG^CGCC NgoMIV 516 GCCGGC G^CCGGC NotI 217 GCGGCCGC GC^GGCCGC PflMI 186 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 278 ACCGGT A^CCGGT PstI 64 CTGCAG CTGCA^G PvuII 448 CAGCTG CAG^CTG SexAI 596 ACCTGGT A^CC[AT]GGT SfcI 64 CTGCAG C^T[GA][CT]AG SfoI 115 GGCGCC GGC^GCC SmlI 484 CTCAAG C^T[CT][GA]AG TfiI 246 GAATC G^A[AT]TC Tsp45I 639 GTCAC ^GT[GC]AC Van91I 186 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 54 GACGTC G[GA]^CG[CT]C 115 GGCGCC AfaI 204 GTAC GT^AC 489 GTAC AspI 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC 415 GACCGTGTC BcnI 90 CCCGG CC^[GC]GG 210 CCGGG BpmI 271 CTGGAG CTGGAG(16/14) 451 CTGGAG BsaHI 54 GACGTC G[GA]^CG[CT]C 115 GGCGCC BsaI 541 GAGACC GGTCTC(1/5) 583 GAGACC BsgI 390 GTGCAG GTGCAG(16/14) 445 GTGCAG BsiEI 218 CGGCCG CG[GA][CT]^CG 518 CGGCCG BstAPI 308 GCAAAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 507 GCAGGATGTGC BstYI 479 AGATCC [GA]^GATC[CT] 685 AGATCT EaeI 218 CGGCCG [CT]^GGCC[GA] 518 CGGCCG EagI 218 CGGCCG C^GGCCG 518 CGGCCG EarI 319 CTCTTC CTCTTC(1/4) 385 GAAGAG EclXI 218 CGGCCG C^GGCCG 518 CGGCCG Eco52I 218 CGGCCG C^GGCCG 518 CGGCCG EcoT14I 365 CCTTGG C^C[AT][AT]GG 427 CCAAGG FauI 174 GCGGG CCCGC(4/6) 370 GCGGG Hin1I 54 GACGTC G[GA]^CG[CT]C 115 GGCGCC HphI 233 TCACC GGTGA(8/7) 249 TCACC Ksp632I 319 CTCTTC CTCTTC(1/4) 385 GAAGAG MaeII 44 ACGT A^CGT 55 ACGT MflI 479 AGATCC [GA]^GATC[CT] 685 AGATCT NciI 90 CCCGG CC^[GC]GG 210 CCGGG NlaIII 39 CATG CATG^ 201 CATG PflFI 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC 415 GACCGTGTC RsaI 204 GTAC GT^AC 489 GTAC SfaNI 457 GATGC GCATC(5/9) 495 GATGC StyI 365 CCTTGG C^C[AT][AT]GG 427 CCAAGG Tth111I 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC 415 GACCGTGTC XhoII 479 AGATCC [GA]^GATC[CT] 685 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AluI AvaII BanI BbvI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI TseI TspRI