(Circular) (Six-base) MAPSORT of: pFN21AE1351.seq Check: 6406 from: 1 to: 6616 pFN21AE1351 6616 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4426 278 Size: 53 83 186 936 2890 2468 Fragments arranged by size: 2890 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3959 1937 Size: 2022 4594 Acc65I G'GTAC_C Cuts at: 2467 3937 2467 Size: 1470 5146 AclI AA'CG_TT Cuts at: 1563 4743 5116 1563 Size: 3180 373 3063 Fragments arranged by size: 3180 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2924 3550 4679 6063 6331 1632 Size: 1292 626 1129 1384 268 1917 Fragments arranged by size: 1917 1384 1292 1129 626 268 AflII C'TTAA_G Cuts at: 820 1017 2863 820 Size: 197 1846 4573 Fragments arranged by size: 4573 1846 197 AflIII A'CryG_T Cuts at: 1203 5515 6492 1203 Size: 4312 977 1327 Fragments arranged by size: 4312 1327 977 AgeI A'CCGG_T Cuts at: 5447 5447 Size: 6616 AhdI GACnn_n'nnGTC Cuts at: 5345 5345 Size: 6616 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6616 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6616 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2721 2753 2777 2809 2721 Size: 32 24 32 6528 Fragments arranged by size: 6528 32 32 24 AlwNI CAG_nnn'CTG Cuts at: 2335 2947 3085 4359 5931 2335 Size: 612 138 1274 1572 3020 Fragments arranged by size: 3020 1572 1274 612 138 ApaLI G'TGCA_C Cuts at: 4673 5829 4673 Size: 1156 5460 ApoI r'AATT_y Cuts at: 1493 2338 3620 3925 4151 4303 6412 1493 Size: 845 1282 305 226 152 2109 1697 Fragments arranged by size: 2109 1697 1282 845 305 226 152 AseI AT'TA_AT Cuts at: 160 1032 5170 160 Size: 872 4138 1606 Fragments arranged by size: 4138 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6616 AvaI C'yCGr_G Cuts at: 1870 1943 3398 3941 1870 Size: 73 1455 543 4545 Fragments arranged by size: 4545 1455 543 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3060 3093 3060 Size: 33 6583 BamHI G'GATC_C Cuts at: 3946 4313 3946 Size: 367 6249 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2467 2479 2807 3937 5392 Size: 325 217 627 680 12 328 1130 1455 Cuts at: 5392 618 Size: 1842 Fragments arranged by size: 1842 1455 1130 680 627 328 325 217 12 BanII G_rGCy'C Cuts at: 1391 2679 3320 3364 3935 1391 Size: 1288 641 44 571 4072 Fragments arranged by size: 4072 1288 641 571 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2131 2351 2925 928 Size: 971 232 220 574 4619 Fragments arranged by size: 4619 971 574 232 220 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4807 4841 1903 Size: 34 2870 34 3678 Fragments arranged by size: 3678 2870 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3061 3713 4508 5718 3061 Size: 652 795 1210 3959 Fragments arranged by size: 3959 1210 795 652 BclI T'GATC_A Cuts at: 1548 1548 Size: 6616 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3146 5227 Size: 107 122 71 631 605 337 1137 2081 Cuts at: 5227 136 Size: 1525 Fragments arranged by size: 2081 1525 1137 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6611 6611 Size: 6616 BlpI GC'TnA_GC Cuts at: 4033 4033 Size: 6616 Bme1580I G_kGCm'C Cuts at: 1790 4677 5833 1790 Size: 2887 1156 2573 Fragments arranged by size: 2887 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6616 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3487 4347 5305 Size: 416 53 83 399 24 2070 860 958 Cuts at: 5305 442 Size: 1753 Fragments arranged by size: 2070 1753 958 860 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6616 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2854 3736 2854 Size: 882 5734 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3264 5276 1239 Size: 2025 2012 2579 Fragments arranged by size: 2579 2025 2012 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2762 4741 5606 5904 2762 Size: 1979 865 298 3474 Fragments arranged by size: 3474 1979 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5279 882 Size: 617 3780 2219 Fragments arranged by size: 3780 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3782 4407 493 Size: 711 2578 625 2702 Fragments arranged by size: 2702 2578 711 625 BsaBI GATnn'nnATC Cuts at: 6610 6610 Size: 6616 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4423 4805 5440 275 Size: 53 83 186 936 2890 382 635 1451 Fragments arranged by size: 2890 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2580 5049 5447 5721 5868 6279 2580 Size: 2469 398 274 147 411 2917 Fragments arranged by size: 2917 2469 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2543 2573 2777 2807 3706 Size: 30 165 30 1140 30 204 30 899 Cuts at: 3706 3736 1178 Size: 30 4058 Fragments arranged by size: 4058 1140 899 204 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2549 2564 2765 3074 1186 Size: 1363 15 201 309 4728 Fragments arranged by size: 4728 1363 309 201 15 BseYI C'CCAG_C Cuts at: 3094 3363 3549 5819 6315 3094 Size: 269 186 2270 496 3395 Fragments arranged by size: 3395 2270 496 269 186 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2810 3506 3528 1163 Size: 573 1074 696 22 4251 Fragments arranged by size: 4251 1074 696 573 22 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2022 3154 4066 4827 5855 1540 Size: 275 186 21 1132 912 761 1028 2301 Fragments arranged by size: 2301 1132 1028 912 761 275 186 21 BsiHKAI G_wGCw'C Cuts at: 4677 4762 5833 4677 Size: 85 1071 5460 Fragments arranged by size: 5460 1071 85 BsmI GAATG_Cn' Cuts at: 2518 4127 4220 2518 Size: 1609 93 4914 Fragments arranged by size: 4914 1609 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2679 3320 3364 3935 4677 4762 5833 Size: 399 889 641 44 571 742 85 1071 Cuts at: 5833 1391 Size: 2174 Fragments arranged by size: 2174 1071 889 742 641 571 399 85 44 BspEI T'CCGG_A Cuts at: 2580 6279 2580 Size: 3699 2917 BspHI T'CATG_A Cuts at: 4505 6235 4505 Size: 1730 4886 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2185 3971 844 Size: 1341 1786 3489 Fragments arranged by size: 3489 1786 1341 BsrBI CCG'CTC Cuts at: 1394 1823 3897 4503 1394 Size: 429 2074 606 3507 Fragments arranged by size: 3507 2074 606 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5111 5285 65 Size: 1183 3863 174 1396 Fragments arranged by size: 3863 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3153 3659 5260 5447 1700 Size: 110 1343 506 1601 187 2869 Fragments arranged by size: 2869 1601 1343 506 187 110 BsrGI T'GTAC_A Cuts at: 96 3444 96 Size: 3348 3268 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6616 BssSI C'ACGA_G Cuts at: 4676 5688 4676 Size: 1012 5604 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6616 BstBI TT'CG_AA Cuts at: 5420 5420 Size: 6616 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6616 BstYI r'GATC_y Cuts at: 1972 3583 3946 4313 4698 4715 6167 6253 6265 Size: 1611 363 367 385 17 1452 86 12 Cuts at: 6265 6611 1972 Size: 346 1977 Fragments arranged by size: 1977 1611 1452 385 367 363 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3486 3486 Size: 6616 BtgI C'CryG_G Cuts at: 513 1065 2004 2267 3195 513 Size: 552 939 263 928 3934 Fragments arranged by size: 3934 939 928 552 263 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2027 3829 487 Size: 683 56 801 1802 3274 Fragments arranged by size: 3274 1802 801 683 56 BtsI GCAGTG_nn' Cuts at: 2619 4132 4926 4946 2619 Size: 1513 794 20 4289 Fragments arranged by size: 4289 1513 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6616 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3177 3212 529 Size: 35 2613 35 3933 Fragments arranged by size: 3933 2613 35 35 DraI TTT'AAA Cuts at: 3920 3920 Size: 6616 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3299 5623 809 Size: 507 1983 2324 1802 Fragments arranged by size: 2324 1983 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2319 2406 Size: 54 1425 50 275 18 273 216 87 Cuts at: 2406 2437 4063 4952 6581 8 Size: 31 1626 889 1629 43 Fragments arranged by size: 1629 1626 1425 889 275 273 216 87 54 50 43 31 18 EagI C'GGCC_G Cuts at: 1537 1812 4063 1537 Size: 275 2251 4090 Fragments arranged by size: 4090 2251 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2452 3051 3436 3758 4546 1353 Size: 1099 599 385 322 788 3423 Fragments arranged by size: 3423 1099 788 599 385 322 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3172 5203 5577 5723 387 Size: 1064 138 1583 2031 374 146 1280 Fragments arranged by size: 2031 1583 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2924 3264 3550 4679 5276 6063 6331 Size: 393 1292 340 286 1129 597 787 268 Cuts at: 6331 1239 Size: 1524 Fragments arranged by size: 1524 1292 1129 787 597 393 340 286 268 EcoNI CCTnn'n_nnAGG Cuts at: 2384 2384 Size: 6616 EcoO109I rG'GnC_Cy Cuts at: 2059 2488 2992 3110 2059 Size: 429 504 118 5565 Fragments arranged by size: 5565 504 429 118 EcoRI G'AATT_C Cuts at: 3925 4303 3925 Size: 378 6238 FspI TGC'GCA Cuts at: 5122 5122 Size: 6616 HaeII r_GCGC'y Cuts at: 1678 5763 1678 Size: 4085 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2627 2659 2941 2973 3691 Size: 32 135 32 1055 32 282 32 718 Cuts at: 3691 3706 3723 3738 5257 5289 5331 5363 1373 Size: 15 17 15 1519 32 42 32 2626 Fragments arranged by size: 2626 1519 1055 718 282 135 42 32 32 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 3960 4206 669 Size: 988 281 2022 246 3079 Fragments arranged by size: 3079 2022 988 281 246 HindIII A'AGCT_T Cuts at: 748 2258 748 Size: 1510 5106 HpaI GTT'AAC Cuts at: 4206 4206 Size: 6616 KpnI G_GTAC'C Cuts at: 2471 3941 2471 Size: 1470 5146 MfeI C'AATT_G Cuts at: 4215 4215 Size: 6616 MluI A'CGCG_T Cuts at: 6492 6492 Size: 6616 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2763 5730 5914 1305 Size: 1458 2967 184 2007 Fragments arranged by size: 2967 2007 1458 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2105 2408 2439 10 Size: 54 1425 343 273 303 31 4187 Fragments arranged by size: 4187 1425 343 303 273 54 31 MslI CAynn'nnrTG Cuts at: 518 1166 3800 4574 4933 5092 518 Size: 648 2634 774 359 159 2042 Fragments arranged by size: 2634 2042 774 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2052 4032 4709 5857 6102 1606 Size: 446 1980 677 1148 245 2120 Fragments arranged by size: 2120 1980 1148 677 446 245 NaeI GCC'GGC Cuts at: 3661 3661 Size: 6616 NcoI C'CATG_G Cuts at: 513 1065 2004 2267 513 Size: 552 939 263 4862 Fragments arranged by size: 4862 939 552 263 NdeI CA'TA_TG Cuts at: 387 2974 387 Size: 2587 4029 NgoMIV G'CCGG_C Cuts at: 3659 3659 Size: 6616 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6616 NotI GC'GGCC_GC Cuts at: 4063 4063 Size: 6616 NspI r_CATG'y Cuts at: 1132 1229 2134 3974 5519 1132 Size: 97 905 1840 1545 2229 Fragments arranged by size: 2229 1840 1545 905 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6616 PciI A'CATG_T Cuts at: 5515 5515 Size: 6616 PflMI CCAn_nnn'nTGG Cuts at: 1108 2457 2854 1108 Size: 1349 397 4870 Fragments arranged by size: 4870 1349 397 PmeI GTTT'AAAC Cuts at: 3920 3920 Size: 6616 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2777 2809 2816 2848 4685 4717 6198 6230 2777 Size: 32 7 32 1837 32 1481 32 3163 Fragments arranged by size: 3163 1837 1481 32 32 32 32 7 PpuMI rG'GwC_Cy Cuts at: 2059 2488 2992 3110 2059 Size: 429 504 118 5565 Fragments arranged by size: 5565 504 429 118 PshAI GACnn'nnGTC Cuts at: 1613 3248 1613 Size: 1635 4981 PsiI TTA'TAA Cuts at: 4186 4186 Size: 6616 PstI C_TGCA'G Cuts at: 830 2931 3968 830 Size: 2101 1037 3478 Fragments arranged by size: 3478 2101 1037 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6616 SalI G'TCGA_C Cuts at: 1936 3958 1936 Size: 2022 4594 SanDI GG'GwC_CC Cuts at: 3110 3110 Size: 6616 SbfI CC_TGCA'GG Cuts at: 3968 3968 Size: 6616 ScaI AGT'ACT Cuts at: 3645 4864 6576 3645 Size: 1219 1712 3685 Fragments arranged by size: 3685 1712 1219 SfcI C'TryA_G Cuts at: 826 1045 2596 2927 3463 3964 5099 5780 5971 Size: 219 1551 331 536 501 1135 681 191 Cuts at: 5971 826 Size: 1471 Fragments arranged by size: 1551 1471 1135 681 536 501 331 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6616 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6616 SmaI CCC'GGG Cuts at: 1872 3400 3943 1872 Size: 1528 543 4545 Fragments arranged by size: 4545 1528 543 SmlI C'TyrA_G Cuts at: 820 1017 1943 2777 2863 4720 5621 5883 820 Size: 197 926 834 86 1857 901 262 1553 Fragments arranged by size: 1857 1553 926 901 834 262 197 86 SnaBI TAC'GTA Cuts at: 493 4407 493 Size: 3914 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6616 SphI G_CATG'C Cuts at: 1132 2134 3974 1132 Size: 1002 1840 3774 Fragments arranged by size: 3774 1840 1002 SspI AAT'ATT Cuts at: 5 52 4540 5 Size: 47 4488 2081 Fragments arranged by size: 4488 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6616 StyI C'CwwG_G Cuts at: 513 1065 2004 2267 2472 2907 3615 4055 513 Size: 552 939 263 205 435 708 440 3074 Fragments arranged by size: 3074 939 708 552 440 435 263 205 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3060 3262 4643 4660 4813 4998 5443 Size: 1255 1480 202 1381 17 153 185 445 Cuts at: 5443 325 Size: 1498 Fragments arranged by size: 1498 1480 1381 1255 445 202 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2538 3405 3444 Size: 274 80 33 51 1443 561 867 39 Cuts at: 3444 3643 4862 6574 96 Size: 199 1219 1712 138 Fragments arranged by size: 1712 1443 1219 867 561 274 199 138 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2543 2575 2607 3102 3134 3225 3257 6576 6608 Size: 32 32 495 32 91 32 3319 32 Cuts at: 6608 2543 Size: 2551 Fragments arranged by size: 3319 2551 495 91 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6616 XbaI T'CTAG_A Cuts at: 3952 3952 Size: 6616 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2432 2011 Size: 421 6195 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6616 XmaI C'CCGG_G Cuts at: 1870 3398 3941 1870 Size: 1528 543 4545 Fragments arranged by size: 4545 1528 543 XmnI GAAnn'nnTTC Cuts at: 2260 2831 4745 2260 Size: 571 1914 4131 Fragments arranged by size: 4131 1914 571 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4424 276 Size: 53 83 186 936 2890 2468 Fragments arranged by size: 2890 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI