(Circular) (Six-base) MAPSORT of: pFN21AE2271.seq Check: 8823 from: 1 to: 6079 pFN21AE2271 6079 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2409 2409 Size: 6079 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3889 278 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3422 1937 Size: 1485 4594 Acc65I G'GTAC_C Cuts at: 3400 3400 Size: 6079 AclI AA'CG_TT Cuts at: 1563 4206 4579 1563 Size: 2643 373 3063 Fragments arranged by size: 3063 2643 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2390 2792 4142 5526 5794 1632 Size: 758 402 1350 1384 268 1917 Fragments arranged by size: 1917 1384 1350 758 402 268 AflII C'TTAA_G Cuts at: 820 1017 2300 820 Size: 197 1283 4599 Fragments arranged by size: 4599 1283 197 AflIII A'CryG_T Cuts at: 1203 3365 4978 5955 1203 Size: 2162 1613 977 1327 Fragments arranged by size: 2162 1613 1327 977 AgeI A'CCGG_T Cuts at: 3351 4910 3351 Size: 1559 4520 AhdI GACnn_n'nnGTC Cuts at: 4808 4808 Size: 6079 AleI CACnn'nnGTG Cuts at: 1166 3182 1166 Size: 2016 4063 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2579 1184 Size: 1395 4684 AlwNI CAG_nnn'CTG Cuts at: 2419 3115 3139 3822 5394 2419 Size: 696 24 683 1572 3104 Fragments arranged by size: 3104 1572 696 683 24 ApaLI G'TGCA_C Cuts at: 4136 5292 4136 Size: 1156 4923 ApoI r'AATT_y Cuts at: 1493 2977 3388 3614 3766 5875 1493 Size: 1484 411 226 152 2109 1697 Fragments arranged by size: 2109 1697 1484 411 226 152 AseI AT'TA_AT Cuts at: 160 1032 4633 160 Size: 872 3601 1606 Fragments arranged by size: 3601 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 3127 3404 1870 Size: 73 1184 277 4545 Fragments arranged by size: 4545 1184 277 73 BamHI G'GATC_C Cuts at: 3409 3776 3409 Size: 367 5712 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2543 3267 3273 3282 3400 Size: 325 217 627 756 724 6 9 118 Cuts at: 3400 4855 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 756 724 627 325 217 118 9 6 BanII G_rGCy'C Cuts at: 1391 2204 2441 2532 3398 1391 Size: 813 237 91 866 4072 Fragments arranged by size: 4072 866 813 237 91 BbeI G_GCGC'C Cuts at: 3286 3286 Size: 6079 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2316 2510 3338 3368 928 Size: 971 417 194 828 30 3639 Fragments arranged by size: 3639 971 828 417 194 30 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3039 3073 4270 4304 1903 Size: 34 1102 34 1197 34 3678 Fragments arranged by size: 3678 1197 1102 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2101 3971 5181 2101 Size: 1870 1210 2999 Fragments arranged by size: 2999 1870 1210 BclI T'GATC_A Cuts at: 1548 2286 1548 Size: 738 5341 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4690 136 Size: 107 122 71 631 605 3018 1525 Fragments arranged by size: 3018 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6074 6074 Size: 6079 BlpI GC'TnA_GC Cuts at: 3023 3496 3023 Size: 473 5606 Bme1580I G_kGCm'C Cuts at: 1790 3347 4140 5296 1790 Size: 1557 793 1156 2573 Fragments arranged by size: 2573 1557 1156 793 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6079 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3810 4768 442 Size: 416 53 83 399 24 2393 958 1753 Fragments arranged by size: 2393 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2612 1056 Size: 1556 4523 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2979 2979 Size: 6079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2516 2546 2611 2804 2954 3056 4739 1239 Size: 1277 30 65 193 150 102 1683 2579 Fragments arranged by size: 2579 1683 1277 193 150 102 65 30 Bpu10I CC'TnA_GC Cuts at: 2814 3012 2814 Size: 198 5881 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2233 4204 5069 5367 2233 Size: 1971 865 298 2945 Fragments arranged by size: 2945 1971 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3061 4742 882 Size: 617 1562 1681 2219 Fragments arranged by size: 2219 1681 1562 617 BsaAI yAC'GTr Cuts at: 493 1204 3870 493 Size: 711 2666 2702 Fragments arranged by size: 2702 2666 711 BsaBI GATnn'nnATC Cuts at: 6073 6073 Size: 6079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3216 3283 3886 4268 Size: 53 83 186 936 1683 67 603 382 Cuts at: 4268 4903 275 Size: 635 1451 Fragments arranged by size: 1683 1451 936 635 603 382 186 83 67 53 BsaWI w'CCGG_w Cuts at: 3351 4512 4910 5184 5331 5742 3351 Size: 1161 398 274 147 411 3688 Fragments arranged by size: 3688 1161 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2040 2070 1178 Size: 30 165 30 637 30 5187 Fragments arranged by size: 5187 637 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2366 2734 3308 1186 Size: 862 318 368 574 3957 Fragments arranged by size: 3957 862 574 368 318 BseYI C'CCAG_C Cuts at: 2062 2092 3208 5282 5778 2062 Size: 30 1116 2074 496 2363 Fragments arranged by size: 2363 2074 1116 496 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3206 1163 Size: 573 1470 4036 Fragments arranged by size: 4036 1470 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3529 4290 5318 1540 Size: 275 186 1528 761 1028 2301 Fragments arranged by size: 2301 1528 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2532 4140 4225 5296 2532 Size: 1608 85 1071 3315 Fragments arranged by size: 3315 1608 1071 85 BsmI GAATG_Cn' Cuts at: 3590 3683 3590 Size: 93 5986 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2204 2441 2532 3347 3398 4140 4225 Size: 399 414 237 91 815 51 742 85 Cuts at: 4225 5296 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 815 742 414 399 237 91 85 51 BspEI T'CCGG_A Cuts at: 5742 5742 Size: 6079 BspHI T'CATG_A Cuts at: 3968 5698 3968 Size: 1730 4349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2409 3344 3434 844 Size: 1565 935 90 3489 Fragments arranged by size: 3489 1565 935 90 BsrBI CCG'CTC Cuts at: 1394 1823 2842 3295 3966 1394 Size: 429 1019 453 671 3507 Fragments arranged by size: 3507 1019 671 453 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2471 4574 4748 65 Size: 1183 1223 2103 174 1396 Fragments arranged by size: 2103 1396 1223 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3221 3270 3351 4723 4910 1700 Size: 110 1411 49 81 1372 187 2869 Fragments arranged by size: 2869 1411 1372 187 110 81 49 BsrGI T'GTAC_A Cuts at: 96 2126 96 Size: 2030 4049 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6079 BssSI C'ACGA_G Cuts at: 4139 5151 4139 Size: 1012 5067 BstAPI GCAn_nnn'nTGC Cuts at: 794 3115 794 Size: 2321 3758 BstBI TT'CG_AA Cuts at: 4883 4883 Size: 6079 BstEII G'GTnAC_C Cuts at: 1184 2800 1184 Size: 1616 4463 BstXI CCAn_nnnn'nTGG Cuts at: 2540 2540 Size: 6079 BstYI r'GATC_y Cuts at: 1972 3409 3776 4161 4178 5630 5716 5728 6074 Size: 1437 367 385 17 1452 86 12 346 Cuts at: 6074 1972 Size: 1977 Fragments arranged by size: 1977 1452 1437 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3093 3093 Size: 6079 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5527 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5340 Fragments arranged by size: 5340 683 56 BtsI GCAGTG_nn' Cuts at: 2713 3116 3595 4389 4409 2713 Size: 403 479 794 20 4383 Fragments arranged by size: 4383 794 479 403 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6044 DraI TTT'AAA Cuts at: 3383 3383 Size: 6079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5086 809 Size: 507 3770 1802 Fragments arranged by size: 3770 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3526 4415 6044 Size: 54 1425 50 275 18 1696 889 1629 Cuts at: 6044 8 Size: 43 Fragments arranged by size: 1696 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3526 1537 Size: 275 1714 4090 Fragments arranged by size: 4090 1714 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2138 4009 1353 Size: 785 1871 3423 Fragments arranged by size: 3423 1871 785 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2744 4666 5040 5186 387 Size: 1064 138 1155 1922 374 146 1280 Fragments arranged by size: 1922 1280 1155 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2530 2530 Size: 6079 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2390 2516 2546 2611 2792 2804 2954 Size: 393 758 126 30 65 181 12 150 Cuts at: 2954 3056 4142 4739 5526 5794 1239 Size: 102 1086 597 787 268 1524 Fragments arranged by size: 1524 1086 787 758 597 393 268 181 150 126 102 65 30 12 EcoNI CCTnn'n_nnAGG Cuts at: 2405 2858 2405 Size: 453 5626 EcoO109I rG'GnC_Cy Cuts at: 2809 2999 3136 2809 Size: 190 137 5752 Fragments arranged by size: 5752 190 137 EcoRI G'AATT_C Cuts at: 3388 3766 3388 Size: 378 5701 FspI TGC'GCA Cuts at: 4585 4585 Size: 6079 HaeII r_GCGC'y Cuts at: 1678 2057 3286 5226 1678 Size: 379 1229 1940 2531 Fragments arranged by size: 2531 1940 1229 379 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3284 3316 4720 4752 4794 Size: 32 135 32 1712 32 1404 32 42 Cuts at: 4794 4826 1373 Size: 32 2626 Fragments arranged by size: 2626 1712 1404 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3423 3669 669 Size: 988 281 1485 246 3079 Fragments arranged by size: 3079 1485 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6079 HpaI GTT'AAC Cuts at: 3669 3669 Size: 6079 KasI G'GCGC_C Cuts at: 3282 3282 Size: 6079 KpnI G_GTAC'C Cuts at: 3404 3404 Size: 6079 MfeI C'AATT_G Cuts at: 2360 3678 2360 Size: 1318 4761 MluI A'CGCG_T Cuts at: 5955 5955 Size: 6079 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2196 3174 5193 5377 1305 Size: 891 978 2019 184 2007 Fragments arranged by size: 2019 2007 978 891 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4257 Fragments arranged by size: 4257 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2603 3182 4037 4396 4555 518 Size: 648 1437 579 855 359 159 2042 Fragments arranged by size: 2042 1437 855 648 579 359 159 MspA1I CmG'CkG Cuts at: 1606 2524 3495 4172 5320 5565 1606 Size: 918 971 677 1148 245 2120 Fragments arranged by size: 2120 1148 971 918 677 245 NaeI GCC'GGC Cuts at: 3223 3272 3223 Size: 49 6030 NarI GG'CG_CC Cuts at: 3283 3283 Size: 6079 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5527 NdeI CA'TA_TG Cuts at: 387 387 Size: 6079 NgoMIV G'CCGG_C Cuts at: 3221 3270 3221 Size: 49 6030 NheI G'CTAG_C Cuts at: 1052 2608 1052 Size: 1556 4523 NotI GC'GGCC_GC Cuts at: 3526 3526 Size: 6079 NspI r_CATG'y Cuts at: 1132 1229 2556 3369 3437 4982 1132 Size: 97 1327 813 68 1545 2229 Fragments arranged by size: 2229 1545 1327 813 97 68 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6079 PciI A'CATG_T Cuts at: 3365 4978 3365 Size: 1613 4466 PflMI CCAn_nnn'nTGG Cuts at: 1108 2754 1108 Size: 1646 4433 PfoI T'CCnGG_A Cuts at: 2532 2532 Size: 6079 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4148 4180 5661 5693 4148 Size: 32 1481 32 4534 Fragments arranged by size: 4534 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2809 2999 3136 2809 Size: 190 137 5752 Fragments arranged by size: 5752 190 137 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6079 PsiI TTA'TAA Cuts at: 3649 3649 Size: 6079 PstI C_TGCA'G Cuts at: 830 3431 830 Size: 2601 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6079 PvuII CAG'CTG Cuts at: 2524 2524 Size: 6079 SacI G_AGCT'C Cuts at: 2532 2532 Size: 6079 SalI G'TCGA_C Cuts at: 1936 3421 1936 Size: 1485 4594 SanDI GG'GwC_CC Cuts at: 2999 2999 Size: 6079 SapI GCTCTTCn'nnn_ Cuts at: 2138 2138 Size: 6079 SbfI CC_TGCA'GG Cuts at: 3431 3431 Size: 6079 ScaI AGT'ACT Cuts at: 4327 6039 4327 Size: 1712 4367 SexAI A'CCwGG_T Cuts at: 2943 2943 Size: 6079 SfcI C'TryA_G Cuts at: 826 1045 3427 4562 5243 5434 826 Size: 219 2382 1135 681 191 1471 Fragments arranged by size: 2382 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 3284 3284 Size: 6079 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6079 SmaI CCC'GGG Cuts at: 1872 3406 1872 Size: 1534 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2248 2300 4183 5084 5346 820 Size: 197 926 305 52 1883 901 262 1553 Fragments arranged by size: 1883 1553 926 901 305 262 197 52 SnaBI TAC'GTA Cuts at: 493 3870 493 Size: 3377 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6079 SphI G_CATG'C Cuts at: 1132 3437 1132 Size: 2305 3774 SspI AAT'ATT Cuts at: 5 52 4003 5 Size: 47 3951 2081 Fragments arranged by size: 3951 2081 47 StuI AGG'CCT Cuts at: 1517 2624 2864 1517 Size: 1107 240 4732 Fragments arranged by size: 4732 1107 240 StyI C'CwwG_G Cuts at: 513 1065 2269 2430 2477 3518 513 Size: 552 1204 161 47 1041 3074 Fragments arranged by size: 3074 1204 1041 552 161 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2411 3070 4106 4123 4276 4461 4906 Size: 1255 831 659 1036 17 153 185 445 Cuts at: 4906 325 Size: 1498 Fragments arranged by size: 1498 1255 1036 831 659 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2126 4325 6037 Size: 274 80 33 51 1443 149 2199 1712 Cuts at: 6037 96 Size: 138 Fragments arranged by size: 2199 1712 1443 274 149 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2070 2102 6039 6071 2070 Size: 32 3937 32 2078 Fragments arranged by size: 3937 2078 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6079 XbaI T'CTAG_A Cuts at: 3415 3415 Size: 6079 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6079 XmaI C'CCGG_G Cuts at: 1870 3404 1870 Size: 1534 4545 XmnI GAAnn'nnTTC Cuts at: 4208 4208 Size: 6079 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3887 276 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SfiI SrfI SwaI XcmI