################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanII BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccII 236 CGCG CG^CG BalI 906 TGGCCA TGG^CCA BamHI 568 GGATCC G^GATCC BbeI 328 GGCGCC GGCGC^C BglII 866 AGATCT A^GATCT BspMI 162 GCAGGT ACCTGC(4/8) BsrBI 619 GAGCGG CCGCTC(-3/-3) BstEII 1239 GGTGACC G^GT[ACGT]ACC BstPI 1239 GGTGACC G^GT[ACGT]ACC BstUI 236 CGCG CG^CG EcoO65I 1239 GGTGACC G^GT[ACGT]ACC EcoT14I 47 CCAAGG C^C[AT][AT]GG HphI 1239 GGTGA GGTGA(8/7) KasI 328 GGCGCC G^GCGCC MluNI 906 TGGCCA TGG^CCA MscI 906 TGGCCA TGG^CCA MvnI 236 CGCG CG^CG NaeI 841 GCCGGC GCC^GGC NarI 328 GGCGCC GG^CGCC NgoMIV 841 GCCGGC G^CCGGC PaeR7I 935 CTCGAG C^TCGAG PflMI 846 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 688 CTGCAG CTGCA^G ScaI 899 AGTACT AGT^ACT SfcI 688 CTGCAG C^T[GA][CT]AG SfoI 328 GGCGCC GGC^GCC SmlI 935 CTCGAG C^T[CT][GA]AG StuI 671 AGGCCT AGG^CCT StyI 47 CCAAGG C^C[AT][AT]GG TaqI 936 TCGA T^CGA TthHB8I 936 TCGA T^CGA Van91I 846 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 41 CCAGACCCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 935 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AccI 712 GTCTAC GT^[AC][GT]AC 1165 GTCTAC AlwNI 385 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 847 CAGGCCCTG AocI 129 CCTCAGG CC^T[ACGT]AGG 285 CCTCAGG AspHI 228 GTGCTC G[AT]GC[AT]^C 551 GAGCAC AvaI 935 CTCGAG C^[CT]CG[GA]G 1024 CCCGAG BanI 328 GGCGCC G^G[CT][GA]CC 658 GGTGCC BsgI 160 GTGCAG GTGCAG(16/14) 700 GTGCAG BsiHKAI 228 GTGCTC G[AT]GC[AT]^C 551 GAGCAC BsoBI 935 CTCGAG C^[CT]CG[GA]G 1024 CCCGAG BstXI 450 CCAAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1229 CCAGCGGCATGG Bsu36I 129 CCTCAGG CC^T[ACGT]AGG 285 CCTCAGG Eco81I 129 CCTCAGG CC^T[ACGT]AGG 285 CCTCAGG FauI 67 GCGGG CCCGC(4/6) 325 GCGGG HaeII 328 GGCGCC [GA]GCGC^[CT] 970 GGCGCT MslI 402 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 556 CACCTCTGTG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AvaII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrDI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI XhoII