(Circular) (Six-base) MAPSORT of: pFN21AE4401.seq Check: 9550 from: 1 to: 5956 pFN21AE4401 5956 bp With 182 enzymes: SgfI * March 28, 2012 13:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2718 3171 3299 1937 Size: 781 453 128 4594 Fragments arranged by size: 4594 781 453 128 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4019 5403 5671 1632 Size: 2387 1384 268 1917 Fragments arranged by size: 2387 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 4855 5832 1203 Size: 3652 977 1327 Fragments arranged by size: 3652 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 2565 1166 Size: 1399 4557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2492 1184 Size: 1308 4648 AlwNI CAG_nnn'CTG Cuts at: 2395 2857 3699 5271 2395 Size: 462 842 1572 3080 Fragments arranged by size: 3080 1572 842 462 ApaLI G'TGCA_C Cuts at: 4013 5169 4013 Size: 1156 4800 ApoI r'AATT_y Cuts at: 1493 3265 3491 3643 5752 1493 Size: 1772 226 152 2109 1697 Fragments arranged by size: 2109 1772 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 2940 3029 3281 1870 Size: 73 997 89 252 4545 Fragments arranged by size: 4545 997 252 89 73 BamHI G'GATC_C Cuts at: 2573 3286 3653 2573 Size: 713 367 4876 Fragments arranged by size: 4876 713 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2333 2663 3277 4732 618 Size: 325 217 627 546 330 614 1455 1842 Fragments arranged by size: 1842 1455 627 614 546 330 325 217 BanII G_rGCy'C Cuts at: 1391 3275 1391 Size: 1884 4072 BbeI G_GCGC'C Cuts at: 2337 2337 Size: 5956 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4985 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4147 4181 1903 Size: 34 2210 34 3678 Fragments arranged by size: 3678 2210 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3848 5058 3848 Size: 1210 4746 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2102 2123 2430 Size: 107 122 71 631 605 430 21 307 Cuts at: 2430 2852 3109 4567 136 Size: 422 257 1458 1525 Fragments arranged by size: 1525 1458 631 605 430 422 307 257 122 107 71 21 BglII A'GATC_T Cuts at: 2871 5951 2871 Size: 3080 2876 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 2299 3140 3151 4017 5173 1790 Size: 509 841 11 866 1156 2573 Fragments arranged by size: 2573 1156 866 841 509 11 BmgBI CAC'GTC Cuts at: 1318 2220 2320 2346 1318 Size: 902 100 26 4928 Fragments arranged by size: 4928 902 100 26 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2156 2602 3687 Size: 416 53 83 399 24 739 446 1085 Cuts at: 3687 4645 442 Size: 958 1753 Fragments arranged by size: 1753 1085 958 739 446 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2425 2579 2425 Size: 154 5802 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2029 2420 2779 2915 4616 1239 Size: 790 391 359 136 1701 2579 Fragments arranged by size: 2579 1701 790 391 359 136 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4081 4946 5244 4081 Size: 865 298 4793 Fragments arranged by size: 4793 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4619 882 Size: 617 3120 2219 Fragments arranged by size: 3120 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3747 493 Size: 711 2543 2702 Fragments arranged by size: 2702 2543 711 BsaBI GATnn'nnATC Cuts at: 5950 5950 Size: 5956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2334 3101 3168 3763 Size: 53 83 186 936 801 767 67 595 Cuts at: 3763 4145 4780 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 801 767 635 595 382 186 83 67 53 BsaWI w'CCGG_w Cuts at: 4389 4787 5061 5208 5619 4389 Size: 398 274 147 411 4726 Fragments arranged by size: 4726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2141 2171 1178 Size: 30 165 30 738 30 4963 Fragments arranged by size: 4963 738 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 2227 2291 2774 1186 Size: 937 104 64 483 4368 Fragments arranged by size: 4368 937 483 104 64 BseYI C'CCAG_C Cuts at: 2698 2839 2889 3249 5159 5655 2698 Size: 141 50 360 1910 496 2999 Fragments arranged by size: 2999 1910 496 360 141 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2186 2726 1163 Size: 573 450 540 4393 Fragments arranged by size: 4393 573 540 450 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3406 4167 5195 1540 Size: 275 186 1405 761 1028 2301 Fragments arranged by size: 2301 1405 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2237 2560 4017 4102 5173 2237 Size: 323 1457 85 1071 3020 Fragments arranged by size: 3020 1457 1071 323 85 BsmI GAATG_Cn' Cuts at: 3467 3560 3467 Size: 93 5863 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2299 2560 3140 3151 3275 4017 Size: 399 447 62 261 580 11 124 742 Cuts at: 4017 4102 5173 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 580 447 399 261 124 85 62 11 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2158 3311 844 Size: 1314 1153 3489 Fragments arranged by size: 3489 1314 1153 BsrBI CCG'CTC Cuts at: 1394 1823 2626 3843 1394 Size: 429 803 1217 3507 Fragments arranged by size: 3507 1217 803 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2611 2965 2977 4451 4625 65 Size: 1183 1363 354 12 1474 174 1396 Fragments arranged by size: 1474 1396 1363 1183 354 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 2520 2660 2846 4600 4787 1700 Size: 110 710 140 186 1754 187 2869 Fragments arranged by size: 2869 1754 710 187 186 140 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 4016 5028 4016 Size: 1012 4944 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5956 BstBI TT'CG_AA Cuts at: 4760 4760 Size: 5956 BstEII G'GTnAC_C Cuts at: 1184 3244 1184 Size: 2060 3896 BstXI CCAn_nnnn'nTGG Cuts at: 2462 3241 2462 Size: 779 5177 BstYI r'GATC_y Cuts at: 1972 2573 2871 3201 3286 3653 4038 4055 5507 Size: 601 298 330 85 367 385 17 1452 Cuts at: 5507 5593 5605 5951 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 601 385 367 346 330 298 86 85 17 12 Bsu36I CC'TnA_GG Cuts at: 2135 2291 2135 Size: 156 5800 BtgI C'CryG_G Cuts at: 513 1065 2004 2481 2528 2667 2686 2722 513 Size: 552 939 477 47 139 19 36 3747 Fragments arranged by size: 3747 939 552 477 139 47 36 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5217 Fragments arranged by size: 5217 683 56 BtsI GCAGTG_nn' Cuts at: 2067 3472 4266 4286 2067 Size: 1405 794 20 3737 Fragments arranged by size: 3737 1405 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4963 809 Size: 507 3647 1802 Fragments arranged by size: 3647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2023 2442 2464 Size: 54 1425 50 275 18 193 419 22 Cuts at: 2464 2525 2848 2911 3403 4292 5921 8 Size: 61 323 63 492 889 1629 43 Fragments arranged by size: 1629 1425 889 492 419 323 275 193 63 61 54 50 43 22 18 EagI C'GGCC_G Cuts at: 1537 1812 3403 1537 Size: 275 1591 4090 Fragments arranged by size: 4090 1591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3886 1353 Size: 2533 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4543 4917 5063 387 Size: 1064 138 2954 374 146 1280 Fragments arranged by size: 2954 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2029 2420 2779 2915 4019 4616 5403 Size: 393 397 391 359 136 1104 597 787 Cuts at: 5403 5671 1239 Size: 268 1524 Fragments arranged by size: 1524 1104 787 597 397 393 391 359 268 136 EcoO109I rG'GnC_Cy Cuts at: 2076 2635 2746 2854 2076 Size: 559 111 108 5178 Fragments arranged by size: 5178 559 111 108 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2558 2558 Size: 5956 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 2337 2979 5103 1678 Size: 659 642 2124 2531 Fragments arranged by size: 2531 2124 659 642 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2565 2597 3077 3109 4597 Size: 32 135 32 993 32 480 32 1488 Cuts at: 4597 4629 4671 4703 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1488 993 480 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3300 3546 669 Size: 988 281 1362 246 3079 Fragments arranged by size: 3079 1362 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KasI G'GCGC_C Cuts at: 2333 2333 Size: 5956 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2271 5070 5254 1305 Size: 966 2799 184 2007 Fragments arranged by size: 2799 2007 966 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2913 10 Size: 54 1425 343 1081 3053 Fragments arranged by size: 3053 1425 1081 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2411 2565 3914 4273 4432 518 Size: 648 1245 154 1349 359 159 2042 Fragments arranged by size: 2042 1349 1245 648 359 159 154 MspA1I CmG'CkG Cuts at: 1606 2155 2271 2698 2843 3063 3237 3372 4049 Size: 549 116 427 145 220 174 135 677 Cuts at: 4049 5197 5442 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 549 427 245 220 174 145 135 116 NaeI GCC'GGC Cuts at: 2848 2848 Size: 5956 NarI GG'CG_CC Cuts at: 2334 2334 Size: 5956 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4465 Fragments arranged by size: 4465 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NgoMIV G'CCGG_C Cuts at: 2846 2846 Size: 5956 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 3314 4859 1132 Size: 97 2085 1545 2229 Fragments arranged by size: 2229 2085 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5956 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 2857 1108 Size: 1749 4207 PfoI T'CCnGG_A Cuts at: 2619 2931 3192 2619 Size: 312 261 5383 Fragments arranged by size: 5383 312 261 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2076 2635 2746 2076 Size: 559 111 5286 Fragments arranged by size: 5286 559 111 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PspXI vC'TCGA_Gb Cuts at: 2940 2940 Size: 5956 PstI C_TGCA'G Cuts at: 830 2697 3308 830 Size: 1867 611 3478 Fragments arranged by size: 3478 1867 611 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 PvuII CAG'CTG Cuts at: 2271 2698 2843 2271 Size: 427 145 5384 Fragments arranged by size: 5384 427 145 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SanDI GG'GwC_CC Cuts at: 2076 2746 2076 Size: 670 5286 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 2906 4204 5916 2906 Size: 1298 1712 2946 Fragments arranged by size: 2946 1712 1298 SfcI C'TryA_G Cuts at: 826 1045 2693 3304 4439 5120 5311 826 Size: 219 1648 611 1135 681 191 1471 Fragments arranged by size: 1648 1471 1135 681 611 219 191 SfoI GGC'GCC Cuts at: 2335 2335 Size: 5956 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 3283 1872 Size: 1411 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2940 4060 4961 5223 820 Size: 197 926 997 1120 901 262 1553 Fragments arranged by size: 1553 1120 997 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 2678 1517 Size: 1161 4795 StyI C'CwwG_G Cuts at: 513 1065 2004 2052 3395 513 Size: 552 939 48 1343 3074 Fragments arranged by size: 3074 1343 939 552 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2402 2584 3983 4000 4153 4338 4783 Size: 1255 822 182 1399 17 153 185 445 Cuts at: 4783 325 Size: 1498 Fragments arranged by size: 1498 1399 1255 822 445 185 182 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2904 4202 5914 Size: 274 80 33 51 1443 927 1298 1712 Cuts at: 5914 96 Size: 138 Fragments arranged by size: 1712 1443 1298 927 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2204 2236 5916 5948 2204 Size: 32 3680 32 2212 Fragments arranged by size: 3680 2212 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5956 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2053 2053 Size: 5956 XhoI C'TCGA_G Cuts at: 1943 2940 1943 Size: 997 4959 XmaI C'CCGG_G Cuts at: 1870 3281 1870 Size: 1411 4545 XmnI GAAnn'nnTTC Cuts at: 4085 4085 Size: 5956 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI