(Linear) (Six-base) MAPSORT of: KIEE4401.seq Check: 4017 from: 1 to: 1251 KIEE4401.orf With 182 enzymes: SgfI * March 22, 2012 17:54 .. AccI GT'mk_AC Cuts at: 0 713 1166 1251 Size: 713 453 85 Fragments arranged by size: 713 453 85 AleI CACnn'nnGTG Cuts at: 0 560 1251 Size: 560 691 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 487 1251 Size: 487 764 AlwNI CAG_nnn'CTG Cuts at: 0 390 852 1251 Size: 390 462 399 Fragments arranged by size: 462 399 390 AvaI C'yCGr_G Cuts at: 0 935 1024 1251 Size: 935 89 227 Fragments arranged by size: 935 227 89 BamHI G'GATC_C Cuts at: 0 568 1251 Size: 568 683 BanI G'GyrC_C Cuts at: 0 328 658 1251 Size: 328 330 593 Fragments arranged by size: 593 330 328 BbeI G_GCGC'C Cuts at: 0 332 1251 Size: 332 919 BglI GCCn_nnn'nGGC Cuts at: 0 97 118 425 847 1104 1251 Size: 97 21 307 422 257 147 Fragments arranged by size: 422 307 257 147 97 21 BglII A'GATC_T Cuts at: 0 866 1251 Size: 866 385 Bme1580I G_kGCm'C Cuts at: 0 294 1135 1146 1251 Size: 294 841 11 105 Fragments arranged by size: 841 294 105 11 BmgBI CAC'GTC Cuts at: 0 215 315 341 1251 Size: 215 100 26 910 Fragments arranged by size: 910 215 100 26 BmrI ACTGGGnnnn_n' Cuts at: 0 151 597 1251 Size: 151 446 654 Fragments arranged by size: 654 446 151 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 420 574 1251 Size: 420 154 677 Fragments arranged by size: 677 420 154 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 24 415 774 910 1251 Size: 24 391 359 136 341 Fragments arranged by size: 391 359 341 136 24 BsaHI Gr'CG_yC Cuts at: 0 329 1096 1163 1251 Size: 329 767 67 88 Fragments arranged by size: 767 329 88 67 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 136 166 1251 Size: 136 30 1085 Fragments arranged by size: 1085 136 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 118 222 286 769 1251 Size: 118 104 64 483 482 Fragments arranged by size: 483 482 118 104 64 BseYI C'CCAG_C Cuts at: 0 693 834 884 1244 1251 Size: 693 141 50 360 7 Fragments arranged by size: 693 360 141 50 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 181 721 1251 Size: 181 540 530 Fragments arranged by size: 540 530 181 BsiHKAI G_wGCw'C Cuts at: 0 232 555 1251 Size: 232 323 696 Fragments arranged by size: 696 323 232 Bsp1286I G_dGCh'C Cuts at: 0 232 294 555 1135 1146 1251 Size: 232 62 261 580 11 105 Fragments arranged by size: 580 261 232 105 62 11 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 153 1251 Size: 153 1098 BsrBI CCG'CTC Cuts at: 0 621 1251 Size: 621 630 BsrDI GCAATG_nn' Cuts at: 0 606 960 972 1251 Size: 606 354 12 279 Fragments arranged by size: 606 354 279 12 BsrFI r'CCGG_y Cuts at: 0 515 655 841 1251 Size: 515 140 186 410 Fragments arranged by size: 515 410 186 140 BstEII G'GTnAC_C Cuts at: 0 1239 1251 Size: 1239 12 BstXI CCAn_nnnn'nTGG Cuts at: 0 457 1236 1251 Size: 457 779 15 Fragments arranged by size: 779 457 15 BstYI r'GATC_y Cuts at: 0 568 866 1196 1251 Size: 568 298 330 55 Fragments arranged by size: 568 330 298 55 Bsu36I CC'TnA_GG Cuts at: 0 130 286 1251 Size: 130 156 965 Fragments arranged by size: 965 156 130 BtgI C'CryG_G Cuts at: 0 476 523 662 681 717 1251 Size: 476 47 139 19 36 534 Fragments arranged by size: 534 476 139 47 36 19 BtsI GCAGTG_nn' Cuts at: 0 62 1251 Size: 62 1189 EaeI y'GGCC_r Cuts at: 0 18 437 459 520 843 906 1251 Size: 18 419 22 61 323 63 345 Fragments arranged by size: 419 345 323 63 61 22 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 24 415 774 910 1251 Size: 24 391 359 136 341 Fragments arranged by size: 391 359 341 136 24 EcoO109I rG'GnC_Cy Cuts at: 0 71 630 741 849 1251 Size: 71 559 111 108 402 Fragments arranged by size: 559 402 111 108 71 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 553 1251 Size: 553 698 HaeII r_GCGC'y Cuts at: 0 332 974 1251 Size: 332 642 277 Fragments arranged by size: 642 332 277 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 560 592 1072 1104 1251 Size: 560 32 480 32 147 Fragments arranged by size: 560 480 147 32 32 KasI G'GCGC_C Cuts at: 0 328 1251 Size: 328 923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 266 1251 Size: 266 985 MscI TGG'CCA Cuts at: 0 908 1251 Size: 908 343 MslI CAynn'nnrTG Cuts at: 0 406 560 1251 Size: 406 154 691 Fragments arranged by size: 691 406 154 MspA1I CmG'CkG Cuts at: 0 150 266 693 838 1058 1232 1251 Size: 150 116 427 145 220 174 19 Fragments arranged by size: 427 220 174 150 145 116 19 NaeI GCC'GGC Cuts at: 0 843 1251 Size: 843 408 NarI GG'CG_CC Cuts at: 0 329 1251 Size: 329 922 NgoMIV G'CCGG_C Cuts at: 0 841 1251 Size: 841 410 PflMI CCAn_nnn'nTGG Cuts at: 0 852 1251 Size: 852 399 PfoI T'CCnGG_A Cuts at: 0 614 926 1187 1251 Size: 614 312 261 64 Fragments arranged by size: 614 312 261 64 PpuMI rG'GwC_Cy Cuts at: 0 71 630 741 1251 Size: 71 559 111 510 Fragments arranged by size: 559 510 111 71 PspXI vC'TCGA_Gb Cuts at: 0 935 1251 Size: 935 316 PstI C_TGCA'G Cuts at: 0 692 1251 Size: 692 559 PvuII CAG'CTG Cuts at: 0 266 693 838 1251 Size: 266 427 145 413 Fragments arranged by size: 427 413 266 145 SanDI GG'GwC_CC Cuts at: 0 71 741 1251 Size: 71 670 510 Fragments arranged by size: 670 510 71 ScaI AGT'ACT Cuts at: 0 901 1251 Size: 901 350 SfcI C'TryA_G Cuts at: 0 688 1251 Size: 688 563 SfoI GGC'GCC Cuts at: 0 330 1251 Size: 330 921 SmlI C'TyrA_G Cuts at: 0 935 1251 Size: 935 316 StuI AGG'CCT Cuts at: 0 673 1251 Size: 673 578 StyI C'CwwG_G Cuts at: 0 47 1251 Size: 47 1204 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 397 579 1251 Size: 397 182 672 Fragments arranged by size: 672 397 182 TatI w'GTAC_w Cuts at: 0 899 1251 Size: 899 352 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 199 231 1251 Size: 199 32 1020 Fragments arranged by size: 1020 199 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 48 1251 Size: 48 1203 XhoI C'TCGA_G Cuts at: 0 935 1251 Size: 935 316 Enzymes that do cut: AccI AleI AlfI AlwNI AvaI BamHI BanI BbeI BglI BglII Bme1580I BmgBI BmrI BplI BpmI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI Eco57MI EcoO109I FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV PflMI PfoI PpuMI PspXI PstI PvuII SanDI ScaI SfcI SfoI SmlI StuI StyI TaqII TatI TstI XcmI XhoI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BlpI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XmaI XmnI ZraI