################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspHI AvaI AvrII BamHI BbrPI BcgI BciVI BclI BfrI BglI BlnI BsaAI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoT22I FbaI FseI HincII HindII HpaI HphI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcyI 30 GGCGCC G[GA]^CG[CT]C AhdI 422 GACAAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 422 GACAAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1141 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 183 GGTCC G^G[AT]CC AviII 1332 TGCGCA TGC^GCA BanI 30 GGCGCC G^G[CT][GA]CC BbeI 30 GGCGCC GGCGC^C BbsI 1389 GTCTTC GAAGAC(2/6) BbvCI 62 CCTCAGC CCTCAGC(-5/-2) BcnI 486 CCCGG CC^[GC]GG BglII 500 AGATCT A^GATCT BlpI 291 GCTTAGC GC^T[ACGT]AGC Bpu1102I 291 GCTTAGC GC^T[ACGT]AGC BpuAI 1389 GTCTTC GAAGAC(2/6) BsaBI 667 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 30 GGCGCC G[GA]^CG[CT]C BsaI 1709 GAGACC GGTCTC(1/5) BsmI 306 GAATGC GAATGC(1/-1) BstYI 500 AGATCT [GA]^GATC[CT] CelII 291 GCTTAGC GC^T[ACGT]AGC Eam1105I 422 GACAAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1662 CCTGGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1292 GATATC GAT^ATC FspI 1332 TGCGCA TGC^GCA HaeII 30 GGCGCC [GA]GCGC^[CT] HapII 487 CCGG C^CGG HgaI 829 GCGTC GACGC(5/10) Hin1I 30 GGCGCC G[GA]^CG[CT]C HindIII 1056 AAGCTT A^AGCTT HpaII 487 CCGG C^CGG KasI 30 GGCGCC G^GCGCC MamI 667 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 500 AGATCT [GA]^GATC[CT] MslI 115 CACACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 788 CAGCTG C[AC]G^C[GT]G MspI 487 CCGG C^CGG NarI 30 GGCGCC GG^CGCC NciI 486 CCCGG CC^[GC]GG NdeI 728 CATATG CA^TATG PflFI 1141 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 1125 CTGCAG CTGCA^G PvuII 788 CAGCTG CAG^CTG SfoI 30 GGCGCC GGC^GCC SmlI 164 CTCAAG C^T[CT][GA]AG StuI 188 AGGCCT AGG^CCT Tth111I 1141 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1215 CCACCTGCCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 500 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 639 GTAC GT^AC 1341 GTAC AlwNI 1192 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG 1368 CAGCCCCTG BalI 354 TGGCCA TGG^CCA 494 TGGCCA BanII 794 GGGCTC G[GA]GC[CT]^C 1570 GAGCCC BfaI 458 CTAG C^TAG 1064 CTAG BsgI 207 GTGCAG GTGCAG(16/14) 1639 GTGCAG BstXI 897 CCAACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1592 CCAACGAGGTGG DsaI 1377 CCACGG C^C[GA][CT]GG 1574 CCGTGG EcoT14I 386 CCAAGG C^C[AT][AT]GG 1713 CCAAGG MaeI 458 CTAG C^TAG 1064 CTAG MaeII 1184 ACGT A^CGT 1402 ACGT MaeIII 323 GTCAC ^GT[ACGT]AC 1139 GTGAC MluNI 354 TGGCCA TGG^CCA 494 TGGCCA MscI 354 TGGCCA TGG^CCA 494 TGGCCA PflMI 712 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1656 CCAAATCCTGG RsaI 639 GTAC GT^AC 1341 GTAC SfaNI 1024 GCATC GCATC(5/9) 1203 GCATC SspI 452 AATATT AAT^ATT 1006 AATATT StyI 386 CCAAGG C^C[AT][AT]GG 1713 CCAAGG Tsp45I 323 GTCAC ^GT[GC]AC 1139 GTGAC Van91I 712 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1656 CCAAATCCTGG XbaI 457 TCTAGA T^CTAGA 1063 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI Asp700I BbvI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI Ksp632I MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XmnI