(Circular) (Six-base) MAPSORT of: pFN21AE6440.seq Check: 2286 from: 1 to: 6430 pFN21AE6440 6430 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2258 3231 2258 Size: 973 5457 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4240 278 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3773 1937 Size: 1836 4594 Acc65I G'GTAC_C Cuts at: 3751 3751 Size: 6430 AclI AA'CG_TT Cuts at: 1563 4557 4930 1563 Size: 2994 373 3063 Fragments arranged by size: 3063 2994 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2045 3065 3224 3408 4493 5877 6145 1632 Size: 413 1020 159 184 1085 1384 268 1917 Fragments arranged by size: 1917 1384 1085 1020 413 268 184 159 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6233 AflIII A'CryG_T Cuts at: 1203 5329 6306 1203 Size: 4126 977 1327 Fragments arranged by size: 4126 1327 977 AgeI A'CCGG_T Cuts at: 5261 5261 Size: 6430 AhdI GACnn_n'nnGTC Cuts at: 2432 5159 2432 Size: 2727 3703 AleI CACnn'nnGTG Cuts at: 1166 2124 1166 Size: 958 5472 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2237 1184 Size: 1053 5377 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3668 3700 3668 Size: 32 6398 AlwNI CAG_nnn'CTG Cuts at: 3202 3378 4173 5745 3202 Size: 176 795 1572 3887 Fragments arranged by size: 3887 1572 795 176 ApaLI G'TGCA_C Cuts at: 4487 5643 4487 Size: 1156 5274 ApoI r'AATT_y Cuts at: 1493 2405 2744 3050 3214 3479 3739 3965 4117 Size: 912 339 306 164 265 260 226 152 Cuts at: 4117 6226 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 912 339 306 265 260 226 164 152 AseI AT'TA_AT Cuts at: 160 1032 4984 160 Size: 872 3952 1606 Fragments arranged by size: 3952 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 AvaI C'yCGr_G Cuts at: 1870 1943 3755 1870 Size: 73 1812 4545 Fragments arranged by size: 4545 1812 73 BamHI G'GATC_C Cuts at: 3760 4127 3760 Size: 367 6063 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 3751 5206 618 Size: 325 217 627 248 1716 1455 1842 Fragments arranged by size: 1842 1716 1455 627 325 248 217 BanII G_rGCy'C Cuts at: 1391 2803 3579 3749 1391 Size: 1412 776 170 4072 Fragments arranged by size: 4072 1412 776 170 BbeI G_GCGC'C Cuts at: 2039 2039 Size: 6430 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3387 928 Size: 971 1488 3971 Fragments arranged by size: 3971 1488 971 BbvCI CC'TCA_GC Cuts at: 2068 2068 Size: 6430 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4621 4655 1903 Size: 34 50 34 2600 34 3678 Fragments arranged by size: 3678 2600 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4322 5532 4322 Size: 1210 5220 BclI T'GATC_A Cuts at: 1548 1548 Size: 6430 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5041 136 Size: 107 122 71 631 605 3369 1525 Fragments arranged by size: 3369 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2505 6425 2505 Size: 3920 2510 BlpI GC'TnA_GC Cuts at: 2297 3847 2297 Size: 1550 4880 Bme1580I G_kGCm'C Cuts at: 1790 2121 3729 4491 5647 1790 Size: 331 1608 762 1156 2573 Fragments arranged by size: 2573 1608 1156 762 331 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6430 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2677 3143 4161 Size: 416 53 83 399 24 1260 466 1018 Cuts at: 4161 5119 442 Size: 958 1753 Fragments arranged by size: 1753 1260 1018 958 466 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6430 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3460 3460 Size: 6430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2061 2357 3350 3635 5090 1239 Size: 822 296 993 285 1455 2579 Fragments arranged by size: 2579 1455 993 822 296 285 Bpu10I CC'TnA_GC Cuts at: 2068 2068 Size: 6430 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2154 4555 5420 5718 2154 Size: 2401 865 298 2866 Fragments arranged by size: 2866 2401 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3708 5093 882 Size: 617 2209 1385 2219 Fragments arranged by size: 2219 2209 1385 617 BsaAI yAC'GTr Cuts at: 493 1204 4221 493 Size: 711 3017 2702 Fragments arranged by size: 3017 2702 711 BsaBI GATnn'nnATC Cuts at: 2676 6424 2676 Size: 3748 2682 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2036 4237 4619 5254 Size: 53 83 186 936 503 2201 382 635 Cuts at: 5254 275 Size: 1451 Fragments arranged by size: 2201 1451 936 635 503 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4863 5261 5535 5682 6093 4863 Size: 398 274 147 411 5200 Fragments arranged by size: 5200 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2195 2225 2605 2635 3668 Size: 30 165 30 792 30 380 30 1033 Cuts at: 3668 3698 1178 Size: 30 3910 Fragments arranged by size: 3910 1033 792 380 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6430 BseYI C'CCAG_C Cuts at: 2102 2782 2795 3154 5633 6129 2102 Size: 680 13 359 2479 496 2403 Fragments arranged by size: 2479 2403 680 496 359 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2233 3665 1163 Size: 573 497 1432 3928 Fragments arranged by size: 3928 1432 573 497 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3880 4641 5669 1540 Size: 275 186 1879 761 1028 2301 Fragments arranged by size: 2301 1879 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4491 4576 5647 4491 Size: 85 1071 5274 Fragments arranged by size: 5274 1071 85 BsmI GAATG_Cn' Cuts at: 2317 3941 4034 2317 Size: 1624 93 4713 Fragments arranged by size: 4713 1624 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2803 3579 3729 3749 4491 4576 Size: 399 331 682 776 150 20 742 85 Cuts at: 4576 5647 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 776 742 682 399 331 150 85 20 BspEI T'CCGG_A Cuts at: 6093 6093 Size: 6430 BspHI T'CATG_A Cuts at: 4319 6049 4319 Size: 1730 4700 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2229 2258 3231 3785 844 Size: 1385 29 973 554 3489 Fragments arranged by size: 3489 1385 973 554 29 BsrBI CCG'CTC Cuts at: 1394 1823 4317 1394 Size: 429 2494 3507 Fragments arranged by size: 3507 2494 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4925 5099 65 Size: 1183 3677 174 1396 Fragments arranged by size: 3677 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5074 5261 1700 Size: 110 3264 187 2869 Fragments arranged by size: 3264 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6430 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6430 BssSI C'ACGA_G Cuts at: 4490 5502 4490 Size: 1012 5418 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6430 BstBI TT'CG_AA Cuts at: 5234 5234 Size: 6430 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6430 BstXI CCAn_nnnn'nTGG Cuts at: 2909 3604 2909 Size: 695 5735 BstYI r'GATC_y Cuts at: 1972 2505 3760 4127 4512 4529 5981 6067 6079 Size: 533 1255 367 385 17 1452 86 12 Cuts at: 6079 6425 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1255 533 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3382 3579 513 Size: 552 2317 197 3364 Fragments arranged by size: 3364 2317 552 197 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5691 Fragments arranged by size: 5691 683 56 BtsI GCAGTG_nn' Cuts at: 2588 3139 3946 4740 4760 2588 Size: 551 807 794 20 4258 Fragments arranged by size: 4258 807 794 551 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6430 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6395 DraI TTT'AAA Cuts at: 3734 3734 Size: 6430 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5437 809 Size: 507 4121 1802 Fragments arranged by size: 4121 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2359 2499 3310 Size: 54 1425 50 275 18 529 140 811 Cuts at: 3310 3877 4766 6395 8 Size: 567 889 1629 43 Fragments arranged by size: 1629 1425 889 811 567 529 275 140 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3877 1537 Size: 275 2065 4090 Fragments arranged by size: 4090 2065 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2064 2391 3134 3539 4360 1353 Size: 711 327 743 405 821 3423 Fragments arranged by size: 3423 821 743 711 405 327 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3567 3638 5017 5391 5537 387 Size: 1064 138 1978 71 1379 374 146 1280 Fragments arranged by size: 1978 1379 1280 1064 374 146 138 71 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2061 2357 3065 3224 3350 3408 Size: 393 413 16 296 708 159 126 58 Cuts at: 3408 3635 4493 5090 5877 6145 1239 Size: 227 858 597 787 268 1524 Fragments arranged by size: 1524 858 787 708 597 413 393 296 268 227 159 126 58 16 EcoNI CCTnn'n_nnAGG Cuts at: 3671 3671 Size: 6430 EcoRI G'AATT_C Cuts at: 3739 4117 3739 Size: 378 6052 EcoRV GAT'ATC Cuts at: 3299 3299 Size: 6430 FspI TGC'GCA Cuts at: 3339 4936 3339 Size: 1597 4833 HaeII r_GCGC'y Cuts at: 1678 2039 5577 1678 Size: 361 3538 2531 Fragments arranged by size: 3538 2531 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2078 2110 2268 2300 2418 Size: 32 135 32 506 32 158 32 118 Cuts at: 2418 2450 5071 5103 5145 5177 1373 Size: 32 2621 32 42 32 2626 Fragments arranged by size: 2626 2621 506 158 135 118 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3774 4020 669 Size: 988 281 1836 246 3079 Fragments arranged by size: 3079 1836 988 281 246 HindIII A'AGCT_T Cuts at: 748 3061 748 Size: 2313 4117 HpaI GTT'AAC Cuts at: 4020 4020 Size: 6430 KasI G'GCGC_C Cuts at: 2035 2035 Size: 6430 KpnI G_GTAC'C Cuts at: 3755 3755 Size: 6430 MfeI C'AATT_G Cuts at: 4029 4029 Size: 6430 MluI A'CGCG_T Cuts at: 6306 6306 Size: 6430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2920 3063 3210 3423 3471 5544 5728 1305 Size: 1615 143 147 213 48 2073 184 2007 Fragments arranged by size: 2073 2007 1615 213 184 147 143 48 MscI TGG'CCA Cuts at: 10 64 1489 1832 2361 2501 10 Size: 54 1425 343 529 140 3939 Fragments arranged by size: 3939 1425 529 343 140 54 MslI CAynn'nnrTG Cuts at: 518 1166 2124 4388 4747 4906 518 Size: 648 958 2264 359 159 2042 Fragments arranged by size: 2264 2042 958 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2795 3846 4523 5671 5916 1606 Size: 1189 1051 677 1148 245 2120 Fragments arranged by size: 2120 1189 1148 1051 677 245 NarI GG'CG_CC Cuts at: 2036 2036 Size: 6430 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5878 NdeI CA'TA_TG Cuts at: 387 2734 387 Size: 2347 4083 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6430 NotI GC'GGCC_GC Cuts at: 3877 3877 Size: 6430 NspI r_CATG'y Cuts at: 1132 1229 3788 5333 1132 Size: 97 2559 1545 2229 Fragments arranged by size: 2559 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2766 1348 Size: 1418 5012 PciI A'CATG_T Cuts at: 5329 5329 Size: 6430 PflMI CCAn_nnn'nTGG Cuts at: 1108 2723 3667 1108 Size: 1615 944 3871 Fragments arranged by size: 3871 1615 944 PmeI GTTT'AAAC Cuts at: 3734 3734 Size: 6430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3668 3700 4499 4531 6012 6044 3668 Size: 32 799 32 1481 32 4054 Fragments arranged by size: 4054 1481 799 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6430 PsiI TTA'TAA Cuts at: 4000 4000 Size: 6430 PstI C_TGCA'G Cuts at: 830 3134 3782 830 Size: 2304 648 3478 Fragments arranged by size: 3478 2304 648 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6430 PvuII CAG'CTG Cuts at: 2795 2795 Size: 6430 SalI G'TCGA_C Cuts at: 1936 3772 1936 Size: 1836 4594 SbfI CC_TGCA'GG Cuts at: 3782 3782 Size: 6430 ScaI AGT'ACT Cuts at: 4678 6390 4678 Size: 1712 4718 SfcI C'TryA_G Cuts at: 826 1045 2414 2916 3130 3778 4913 5594 5785 Size: 219 1369 502 214 648 1135 681 191 Cuts at: 5785 826 Size: 1471 Fragments arranged by size: 1471 1369 1135 681 648 502 219 214 191 SfoI GGC'GCC Cuts at: 2037 2037 Size: 6430 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6430 SmaI CCC'GGG Cuts at: 1872 3757 1872 Size: 1885 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2169 4534 5435 5697 820 Size: 197 926 226 2365 901 262 1553 Fragments arranged by size: 2365 1553 926 901 262 226 197 SnaBI TAC'GTA Cuts at: 493 4221 493 Size: 3728 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6430 SphI G_CATG'C Cuts at: 1132 3788 1132 Size: 2656 3774 SspI AAT'ATT Cuts at: 5 52 2459 3013 4354 5 Size: 47 2407 554 1341 2081 Fragments arranged by size: 2407 2081 1341 554 47 StuI AGG'CCT Cuts at: 1517 2195 1517 Size: 678 5752 StyI C'CwwG_G Cuts at: 513 1065 2391 3718 3869 513 Size: 552 1326 1327 151 3074 Fragments arranged by size: 3074 1327 1326 552 151 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4457 4474 4627 4812 5257 325 Size: 1255 2877 17 153 185 445 1498 Fragments arranged by size: 2877 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3345 4676 6388 Size: 274 80 33 51 1443 1368 1331 1712 Cuts at: 6388 96 Size: 138 Fragments arranged by size: 1712 1443 1368 1331 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2195 2227 6390 6422 2195 Size: 32 4163 32 2203 Fragments arranged by size: 4163 2203 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3149 1316 Size: 1833 4597 XbaI T'CTAG_A Cuts at: 2462 3068 3766 2462 Size: 606 698 5126 Fragments arranged by size: 5126 698 606 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3227 3227 Size: 6430 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6430 XmaI C'CCGG_G Cuts at: 1870 3755 1870 Size: 1885 4545 XmnI GAAnn'nnTTC Cuts at: 2470 2744 2855 3683 4559 2470 Size: 274 111 828 876 4341 Fragments arranged by size: 4341 876 828 274 111 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4238 276 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI