(Linear) (Six-base) MAPSORT of: KIEE6440.seq Check: 8079 from: 1 to: 1725 KIEE6440.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 253 1226 1725 Size: 253 973 499 Fragments arranged by size: 973 499 253 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 40 1060 1219 1403 1725 Size: 40 1020 159 184 322 Fragments arranged by size: 1020 322 184 159 40 AhdI GACnn_n'nnGTC Cuts at: 0 427 1725 Size: 427 1298 AleI CACnn'nnGTG Cuts at: 0 119 1725 Size: 119 1606 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 232 1725 Size: 232 1493 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1663 1695 1725 Size: 1663 32 30 Fragments arranged by size: 1663 32 30 AlwNI CAG_nnn'CTG Cuts at: 0 1197 1373 1725 Size: 1197 176 352 Fragments arranged by size: 1197 352 176 ApoI r'AATT_y Cuts at: 0 400 739 1045 1209 1474 1725 Size: 400 339 306 164 265 251 Fragments arranged by size: 400 339 306 265 251 164 BanI G'GyrC_C Cuts at: 0 30 1725 Size: 30 1695 BanII G_rGCy'C Cuts at: 0 798 1574 1725 Size: 798 776 151 Fragments arranged by size: 798 776 151 BbeI G_GCGC'C Cuts at: 0 34 1725 Size: 34 1691 BbsI GAAGACnn'nnnn_ Cuts at: 0 1382 1725 Size: 1382 343 BbvCI CC'TCA_GC Cuts at: 0 63 1725 Size: 63 1662 BglII A'GATC_T Cuts at: 0 500 1725 Size: 500 1225 BlpI GC'TnA_GC Cuts at: 0 292 1725 Size: 292 1433 Bme1580I G_kGCm'C Cuts at: 0 116 1724 1725 Size: 116 1608 1 Fragments arranged by size: 1608 116 1 BmrI ACTGGGnnnn_n' Cuts at: 0 672 1138 1725 Size: 672 466 587 Fragments arranged by size: 672 587 466 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1455 1725 Size: 1455 270 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 56 352 1345 1630 1725 Size: 56 296 993 285 95 Fragments arranged by size: 993 296 285 95 56 Bpu10I CC'TnA_GC Cuts at: 0 63 1725 Size: 63 1662 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 149 1725 Size: 149 1576 BsaI GGTCTCn'nnnn_ Cuts at: 0 1703 1725 Size: 1703 22 BsaBI GATnn'nnATC Cuts at: 0 671 1725 Size: 671 1054 BsaHI Gr'CG_yC Cuts at: 0 31 1725 Size: 31 1694 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 190 220 600 630 1663 1693 1725 Size: 190 30 380 30 1033 30 32 Fragments arranged by size: 1033 380 190 32 30 30 30 BseYI C'CCAG_C Cuts at: 0 97 777 790 1149 1725 Size: 97 680 13 359 576 Fragments arranged by size: 680 576 359 97 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 228 1660 1725 Size: 228 1432 65 Fragments arranged by size: 1432 228 65 BsmI GAATG_Cn' Cuts at: 0 312 1725 Size: 312 1413 Bsp1286I G_dGCh'C Cuts at: 0 116 798 1574 1724 1725 Size: 116 682 776 150 1 Fragments arranged by size: 776 682 150 116 1 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 224 253 1226 1725 Size: 224 29 973 499 Fragments arranged by size: 973 499 224 29 BstXI CCAn_nnnn'nTGG Cuts at: 0 904 1599 1725 Size: 904 695 126 Fragments arranged by size: 904 695 126 BstYI r'GATC_y Cuts at: 0 500 1725 Size: 500 1225 BtgI C'CryG_G Cuts at: 0 1377 1574 1725 Size: 1377 197 151 Fragments arranged by size: 1377 197 151 BtsI GCAGTG_nn' Cuts at: 0 583 1134 1725 Size: 583 551 591 Fragments arranged by size: 591 583 551 EaeI y'GGCC_r Cuts at: 0 354 494 1305 1725 Size: 354 140 811 420 Fragments arranged by size: 811 420 354 140 EarI CTCTTCn'nnn_ Cuts at: 0 59 386 1129 1534 1725 Size: 59 327 743 405 191 Fragments arranged by size: 743 405 327 191 59 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1562 1633 1725 Size: 1562 71 92 Fragments arranged by size: 1562 92 71 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 40 56 352 1060 1219 1345 1403 1630 Size: 40 16 296 708 159 126 58 227 Cuts at: 1630 1725 Size: 95 Fragments arranged by size: 708 296 227 159 126 95 58 40 16 EcoNI CCTnn'n_nnAGG Cuts at: 0 1666 1725 Size: 1666 59 EcoRV GAT'ATC Cuts at: 0 1294 1725 Size: 1294 431 FspI TGC'GCA Cuts at: 0 1334 1725 Size: 1334 391 HaeII r_GCGC'y Cuts at: 0 34 1725 Size: 34 1691 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 73 105 263 295 413 445 1725 Size: 73 32 158 32 118 32 1280 Fragments arranged by size: 1280 158 118 73 32 32 32 HindIII A'AGCT_T Cuts at: 0 1056 1725 Size: 1056 669 KasI G'GCGC_C Cuts at: 0 30 1725 Size: 30 1695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 915 1058 1205 1418 1466 1725 Size: 915 143 147 213 48 259 Fragments arranged by size: 915 259 213 147 143 48 MscI TGG'CCA Cuts at: 0 356 496 1725 Size: 356 140 1229 Fragments arranged by size: 1229 356 140 MslI CAynn'nnrTG Cuts at: 0 119 1725 Size: 119 1606 MspA1I CmG'CkG Cuts at: 0 790 1725 Size: 790 935 NarI GG'CG_CC Cuts at: 0 31 1725 Size: 31 1694 NdeI CA'TA_TG Cuts at: 0 729 1725 Size: 729 996 PasI CC'CwG_GG Cuts at: 0 761 1725 Size: 761 964 PflMI CCAn_nnn'nTGG Cuts at: 0 718 1662 1725 Size: 718 944 63 Fragments arranged by size: 944 718 63 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1663 1695 1725 Size: 1663 32 30 Fragments arranged by size: 1663 32 30 PstI C_TGCA'G Cuts at: 0 1129 1725 Size: 1129 596 PvuII CAG'CTG Cuts at: 0 790 1725 Size: 790 935 SfcI C'TryA_G Cuts at: 0 409 911 1125 1725 Size: 409 502 214 600 Fragments arranged by size: 600 502 409 214 SfoI GGC'GCC Cuts at: 0 32 1725 Size: 32 1693 SmlI C'TyrA_G Cuts at: 0 164 1725 Size: 164 1561 SspI AAT'ATT Cuts at: 0 454 1008 1725 Size: 454 554 717 Fragments arranged by size: 717 554 454 StuI AGG'CCT Cuts at: 0 190 1725 Size: 190 1535 StyI C'CwwG_G Cuts at: 0 386 1713 1725 Size: 386 1327 12 Fragments arranged by size: 1327 386 12 TatI w'GTAC_w Cuts at: 0 1340 1725 Size: 1340 385 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 190 222 1725 Size: 190 32 1503 Fragments arranged by size: 1503 190 32 Tth111I GACn'n_nGTC Cuts at: 0 1144 1725 Size: 1144 581 XbaI T'CTAG_A Cuts at: 0 457 1063 1725 Size: 457 606 662 Fragments arranged by size: 662 606 457 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1222 1725 Size: 1222 503 XmnI GAAnn'nnTTC Cuts at: 0 465 739 850 1678 1725 Size: 465 274 111 828 47 Fragments arranged by size: 828 465 274 111 47 Enzymes that do cut: AarI AcuI AhdI AleI AlfI AloI AlwNI ApoI BanI BanII BbeI BbsI BbvCI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseYI BsgI BsmI Bsp1286I BspMI BstXI BstYI BtgI BtsI EaeI EarI EciI Eco57MI EcoNI EcoRV FspI HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I NarI NdeI PasI PflMI PpiI PstI PvuII SfcI SfoI SmlI SspI StuI StyI TatI TstI Tth111I XbaI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglI BmgBI BmtI BsaAI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoO109I EcoRI FalI FseI FspAI HincII HpaI KpnI MfeI MluI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TaqII XhoI XmaI ZraI