################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FbaI FseI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MluI MroI MslI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AfaI 1006 GTAC GT^AC AflII 1269 CTTAAG C^TTAAG AlwI 1305 GATCC GGATC(4/5) AlwNI 51 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 1232 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 222 GAGCAC G[AT]GC[AT]^C AvaII 404 GGTCC G^G[AT]CC AviII 58 TGCGCA TGC^GCA BalI 1071 TGGCCA TGG^CCA BbvI 615 GCAGC GCAGC(8/12) BciVI 1054 GTATCC GTATCC(6/5) BfaI 398 CTAG C^TAG BfrI 1269 CTTAAG C^TTAAG BmyI 222 GAGCAC G[AGT]GC[ACT]^C BsaAI 1348 TACGTA [CT]AC^GT[GA] BsgI 1015 CTGCAC GTGCAG(16/14) BsiHKAI 222 GAGCAC G[AT]GC[AT]^C BsmAI 778 GTCTC GTCTC(1/5) BsmFI 737 GGGAC GGGAC(10/14) Bsp1286I 222 GAGCAC G[AGT]GC[ACT]^C BsrDI 729 GCAATG GCAATG(2/0) BstAPI 347 GCAGGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 59 GCGC GCG^C DraI 1387 TTTAAA TTT^AAA DsaI 864 CCACGG C^C[GA][CT]GG EaeI 1071 TGGCCA [CT]^GGCC[GA] EcoRI 573 GAATTC G^AATTC EcoT14I 1216 CCAAGG C^C[AT][AT]GG FspI 58 TGCGCA TGC^GCA HhaI 59 GCGC GCG^C HinP1I 59 GCGC G^CGC HincII 558 GTTGAC GT[CT]^[GA]AC HindII 558 GTTGAC GT[CT]^[GA]AC MaeI 398 CTAG C^TAG MfeI 526 CAATTG C^AATTG MluNI 1071 TGGCCA TGG^CCA MscI 1071 TGGCCA TGG^CCA MspA1I 757 CAGCTG C[AC]G^C[GT]G MunI 526 CAATTG C^AATTG NlaIV 1173 GGCCCC GG[ACGT]^[ACGT]CC PleI 319 GAGTC GAGTC(4/5) PvuII 757 CAGCTG CAG^CTG RsaI 1006 GTAC GT^AC SfcI 1366 CTACAG C^T[GA][CT]AG SnaBI 1348 TACGTA TAC^GTA SspI 1234 AATATT AAT^ATT StuI 870 AGGCCT AGG^CCT StyI 1216 CCAAGG C^C[AT][AT]GG TseI 615 GCAGC G^C[AT]GC Tsp45I 53 GTCAC ^GT[GC]AC XmnI 1232 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 7 CCGC CCGC(-3/-1) 1112 CCGC BbsI 991 GAAGAC GAAGAC(2/6) 601 GTCTTC BcnI 315 CCGGG CC^[GC]GG 1091 CCCGG BglII 486 AGATCT A^GATCT 720 AGATCT BpmI 1081 CTCCAG CTGGAG(16/14) 292 CTGGAG BpuAI 991 GAAGAC GAAGAC(2/6) 601 GTCTTC BsiYI 534 CCAAATGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 859 CCTGACCACGG BslI 534 CCAAATGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 859 CCTGACCACGG BsmI 343 GAATGC GAATGC(1/-1) 1274 GCATTC BstF5I 543 GGATG GGATG(2/0) 567 GGATG DraII 257 AGGCCCT [GA]G^G[ACGT]CC[CT] 1172 AGGCCCC EarI 102 GAAGAG CTCTTC(1/4) 139 GAAGAG EcoO109I 257 AGGCCCT [GA]G^G[ACGT]CC[CT] 1172 AGGCCCC Fnu4HI 6 GCCGC GC^[ACGT]GC 615 GCAGC FokI 543 GGATG GGATG(9/13) 567 GGATG HapII 315 CCGG C^CGG 1092 CCGG HpaII 315 CCGG C^CGG 1092 CCGG ItaI 6 GCCGC GC^[ACGT]GC 615 GCAGC Ksp632I 102 GAAGAG CTCTTC(1/4) 139 GAAGAG MaeII 599 ACGT A^CGT 1349 ACGT MaeIII 53 GTCAC ^GT[ACGT]AC 888 GTTAC MspI 315 CCGG C^CGG 1092 CCGG NciI 315 CCGGG CC^[GC]GG 1091 CCCGG ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AcsI AluI ApoI BsaJI BspHI BspMI BsrI BssKI BstNI BstYI Cfr13I DdeI DpnII EcoRII HaeIII HinfI HphI MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII RcaI Sau3AI Sau96I ScrFI SfaNI SmlI TfiI Tru9I Tsp509I TspRI XhoII