(Circular) (Six-base) MAPSORT of: pFN21AE6438.seq Check: 3938 from: 1 to: 6103 pFN21AE6438 6103 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3913 278 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3446 1937 Size: 1509 4594 Acc65I G'GTAC_C Cuts at: 3424 3424 Size: 6103 AclI AA'CG_TT Cuts at: 1563 4230 4603 1563 Size: 2667 373 3063 Fragments arranged by size: 3063 2667 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2126 2255 2593 2846 3015 4166 5550 5818 Size: 494 129 338 253 169 1151 1384 268 Cuts at: 5818 1632 Size: 1917 Fragments arranged by size: 1917 1384 1151 494 338 268 253 169 129 AflII C'TTAA_G Cuts at: 820 1017 3274 820 Size: 197 2257 3649 Fragments arranged by size: 3649 2257 197 AflIII A'CryG_T Cuts at: 1203 5002 5979 1203 Size: 3799 977 1327 Fragments arranged by size: 3799 1327 977 AgeI A'CCGG_T Cuts at: 4934 4934 Size: 6103 AhdI GACnn_n'nnGTC Cuts at: 4832 4832 Size: 6103 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6103 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6103 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2537 2569 2537 Size: 32 6071 AlwNI CAG_nnn'CTG Cuts at: 2061 3846 5418 2061 Size: 1785 1572 2746 Fragments arranged by size: 2746 1785 1572 ApaLI G'TGCA_C Cuts at: 4160 5316 4160 Size: 1156 4947 ApoI r'AATT_y Cuts at: 1493 2382 2578 2935 2954 3412 3638 3790 5899 Size: 889 196 357 19 458 226 152 2109 Cuts at: 5899 1493 Size: 1697 Fragments arranged by size: 2109 1697 889 458 357 226 196 152 19 AseI AT'TA_AT Cuts at: 160 1032 4657 160 Size: 872 3625 1606 Fragments arranged by size: 3625 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 AvaI C'yCGr_G Cuts at: 1870 1943 3428 1870 Size: 73 1485 4545 Fragments arranged by size: 4545 1485 73 BamHI G'GATC_C Cuts at: 3433 3800 3433 Size: 367 5736 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3424 4879 618 Size: 325 217 627 1637 1455 1842 Fragments arranged by size: 1842 1637 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3422 1391 Size: 2031 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2599 3003 928 Size: 971 700 404 4028 Fragments arranged by size: 4028 971 700 404 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4294 4328 1903 Size: 34 2357 34 3678 Fragments arranged by size: 3678 2357 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3070 3995 5205 3070 Size: 925 1210 3968 Fragments arranged by size: 3968 1210 925 BclI T'GATC_A Cuts at: 1548 1548 Size: 6103 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4714 136 Size: 107 122 71 631 605 3042 1525 Fragments arranged by size: 3042 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2491 2725 6098 2491 Size: 234 3373 2496 Fragments arranged by size: 3373 2496 234 BlpI GC'TnA_GC Cuts at: 3520 3520 Size: 6103 Bme1580I G_kGCm'C Cuts at: 1790 4164 5320 1790 Size: 2374 1156 2573 Fragments arranged by size: 2573 2374 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6103 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3834 4792 442 Size: 416 53 83 399 24 2417 958 1753 Fragments arranged by size: 2417 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6103 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2318 3071 4763 1239 Size: 1079 753 1692 2579 Fragments arranged by size: 2579 1692 1079 753 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2750 3290 4228 5093 5391 2750 Size: 540 938 865 298 3462 Fragments arranged by size: 3462 938 865 540 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4766 882 Size: 617 3267 2219 Fragments arranged by size: 3267 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3355 3894 493 Size: 711 2151 539 2702 Fragments arranged by size: 2702 2151 711 539 BsaBI GATnn'nnATC Cuts at: 6097 6097 Size: 6103 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3910 4292 4927 275 Size: 53 83 186 936 2377 382 635 1451 Fragments arranged by size: 2377 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4536 4934 5208 5355 5766 4536 Size: 398 274 147 411 4873 Fragments arranged by size: 4873 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2537 2567 1178 Size: 30 165 30 1134 30 4714 Fragments arranged by size: 4714 1134 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6103 BseYI C'CCAG_C Cuts at: 3153 5306 5802 3153 Size: 2153 496 3454 Fragments arranged by size: 3454 2153 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3005 1163 Size: 573 1269 4261 Fragments arranged by size: 4261 1269 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3553 4314 5342 1540 Size: 275 186 1552 761 1028 2301 Fragments arranged by size: 2301 1552 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2231 4164 4249 5320 2231 Size: 1933 85 1071 3014 Fragments arranged by size: 3014 1933 1071 85 BsmI GAATG_Cn' Cuts at: 2354 3279 3614 3707 2354 Size: 925 335 93 4750 Fragments arranged by size: 4750 925 335 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2231 3422 4164 4249 5320 1391 Size: 399 441 1191 742 85 1071 2174 Fragments arranged by size: 2174 1191 1071 742 441 399 85 BspEI T'CCGG_A Cuts at: 5766 5766 Size: 6103 BspHI T'CATG_A Cuts at: 2187 2959 3229 3992 5722 2187 Size: 772 270 763 1730 2568 Fragments arranged by size: 2568 1730 772 763 270 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2343 2402 3022 3458 844 Size: 1499 59 620 436 3489 Fragments arranged by size: 3489 1499 620 436 59 BsrBI CCG'CTC Cuts at: 1394 1823 3990 1394 Size: 429 2167 3507 Fragments arranged by size: 3507 2167 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2741 4598 4772 65 Size: 1183 1493 1857 174 1396 Fragments arranged by size: 1857 1493 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4747 4934 1700 Size: 110 2937 187 2869 Fragments arranged by size: 2937 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6103 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6103 BssSI C'ACGA_G Cuts at: 4163 5175 4163 Size: 1012 5091 BstAPI GCAn_nnn'nTGC Cuts at: 794 2358 794 Size: 1564 4539 BstBI TT'CG_AA Cuts at: 4907 4907 Size: 6103 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6103 BstYI r'GATC_y Cuts at: 1972 2491 2725 3309 3433 3800 4185 4202 5654 Size: 519 234 584 124 367 385 17 1452 Cuts at: 5654 5740 5752 6098 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 584 519 385 367 346 234 124 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2869 513 Size: 552 1804 3747 Fragments arranged by size: 3747 1804 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5364 Fragments arranged by size: 5364 683 56 BtsI GCAGTG_nn' Cuts at: 2059 3619 4413 4433 2059 Size: 1560 794 20 3729 Fragments arranged by size: 3729 1560 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6103 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6068 DraI TTT'AAA Cuts at: 3394 3407 3394 Size: 13 6090 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5110 809 Size: 507 3794 1802 Fragments arranged by size: 3794 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3076 3550 4439 Size: 54 1425 50 275 18 1246 474 889 Cuts at: 4439 6068 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1246 889 474 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3550 1537 Size: 275 1738 4090 Fragments arranged by size: 4090 1738 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2102 2139 4033 1353 Size: 749 37 1894 3423 Fragments arranged by size: 3423 1894 749 37 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4690 5064 5210 387 Size: 1064 138 3101 374 146 1280 Fragments arranged by size: 3101 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2255 2318 2593 2846 3015 3071 Size: 393 494 129 63 275 253 169 56 Cuts at: 3071 4166 4763 5550 5818 1239 Size: 1095 597 787 268 1524 Fragments arranged by size: 1524 1095 787 597 494 393 275 268 253 169 129 63 56 EcoO109I rG'GnC_Cy Cuts at: 2263 3178 2263 Size: 915 5188 EcoRI G'AATT_C Cuts at: 2578 3412 3790 2578 Size: 834 378 4891 Fragments arranged by size: 4891 834 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2744 2744 Size: 6103 FspI TGC'GCA Cuts at: 2065 4609 2065 Size: 2544 3559 HaeII r_GCGC'y Cuts at: 1678 5250 1678 Size: 3572 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4744 4776 4818 4850 1373 Size: 32 135 32 3172 32 42 32 2626 Fragments arranged by size: 3172 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2565 3447 3693 669 Size: 988 281 627 882 246 3079 Fragments arranged by size: 3079 988 882 627 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6103 HpaI GTT'AAC Cuts at: 3693 3693 Size: 6103 KpnI G_GTAC'C Cuts at: 3428 3428 Size: 6103 MfeI C'AATT_G Cuts at: 2531 3702 2531 Size: 1171 4932 MluI A'CGCG_T Cuts at: 5979 5979 Size: 6103 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5217 5401 1305 Size: 3912 184 2007 Fragments arranged by size: 3912 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3078 10 Size: 54 1425 343 1246 3035 Fragments arranged by size: 3035 1425 1246 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4061 4420 4579 518 Size: 648 2895 359 159 2042 Fragments arranged by size: 2895 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2764 3519 4196 5344 5589 1606 Size: 1158 755 677 1148 245 2120 Fragments arranged by size: 2120 1158 1148 755 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5551 NdeI CA'TA_TG Cuts at: 387 387 Size: 6103 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6103 NotI GC'GGCC_GC Cuts at: 3550 3550 Size: 6103 NspI r_CATG'y Cuts at: 1132 1229 3461 5006 1132 Size: 97 2232 1545 2229 Fragments arranged by size: 2232 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6103 PciI A'CATG_T Cuts at: 5002 5002 Size: 6103 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6103 PmeI GTTT'AAAC Cuts at: 3407 3407 Size: 6103 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2537 2569 4172 4204 5685 5717 2537 Size: 32 1603 32 1481 32 2923 Fragments arranged by size: 2923 1603 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6103 PsiI TTA'TAA Cuts at: 3673 3673 Size: 6103 PstI C_TGCA'G Cuts at: 830 3455 830 Size: 2625 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6103 PvuII CAG'CTG Cuts at: 2764 2764 Size: 6103 SalI G'TCGA_C Cuts at: 1936 3445 1936 Size: 1509 4594 SbfI CC_TGCA'GG Cuts at: 3455 3455 Size: 6103 ScaI AGT'ACT Cuts at: 4351 6063 4351 Size: 1712 4391 SfcI C'TryA_G Cuts at: 826 1045 3371 3451 4586 5267 5458 826 Size: 219 2326 80 1135 681 191 1471 Fragments arranged by size: 2326 1471 1135 681 219 191 80 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6103 SmaI CCC'GGG Cuts at: 1872 3430 1872 Size: 1558 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2729 3274 3305 4207 5108 5370 Size: 197 926 786 545 31 902 901 262 Cuts at: 5370 820 Size: 1553 Fragments arranged by size: 1553 926 902 901 786 545 262 197 31 SnaBI TAC'GTA Cuts at: 493 3355 3894 493 Size: 2862 539 2702 Fragments arranged by size: 2862 2702 539 SpeI A'CTAG_T Cuts at: 152 152 Size: 6103 SphI G_CATG'C Cuts at: 1132 3461 1132 Size: 2329 3774 SspI AAT'ATT Cuts at: 5 52 3241 4027 5 Size: 47 3189 786 2081 Fragments arranged by size: 3189 2081 786 47 StuI AGG'CCT Cuts at: 1517 2877 1517 Size: 1360 4743 StyI C'CwwG_G Cuts at: 513 1065 3221 3542 513 Size: 552 2156 321 3074 Fragments arranged by size: 3074 2156 552 321 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2083 3145 4130 4147 4300 4485 4930 Size: 1255 503 1062 985 17 153 185 445 Cuts at: 4930 325 Size: 1498 Fragments arranged by size: 1498 1255 1062 985 503 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4349 6061 96 Size: 274 80 33 51 1443 2372 1712 138 Fragments arranged by size: 2372 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3294 3326 6063 6095 3294 Size: 32 2737 32 3302 Fragments arranged by size: 3302 2737 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6103 XbaI T'CTAG_A Cuts at: 3439 3439 Size: 6103 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6103 XmaI C'CCGG_G Cuts at: 1870 3428 1870 Size: 1558 4545 XmnI GAAnn'nnTTC Cuts at: 3241 4232 3241 Size: 991 5112 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3911 276 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI