(Linear) (Six-base) MAPSORT of: KIEE1457.seq Check: 9208 from: 1 to: 3807 KIEE1457.orf With 182 enzymes: SgfI * December 5, 2014 12:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 533 620 1001 1320 1764 2475 3807 Size: 533 87 381 319 444 711 1332 Fragments arranged by size: 1332 711 533 444 381 319 87 AccI GT'mk_AC Cuts at: 0 3611 3807 Size: 3611 196 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 292 901 1357 2174 2988 3682 3807 Size: 133 159 609 456 817 814 694 125 Fragments arranged by size: 817 814 694 609 456 159 133 125 AflIII A'CryG_T Cuts at: 0 2348 3490 3614 3807 Size: 2348 1142 124 193 Fragments arranged by size: 2348 1142 193 124 AhdI GACnn_n'nnGTC Cuts at: 0 2340 3807 Size: 2340 1467 AleI CACnn'nnGTG Cuts at: 0 13 3807 Size: 13 3794 AlwNI CAG_nnn'CTG Cuts at: 0 171 606 1335 1437 2304 2418 2819 3331 Size: 171 435 729 102 867 114 401 512 Cuts at: 3331 3535 3807 Size: 204 272 Fragments arranged by size: 867 729 512 435 401 272 204 171 114 102 ApaI G_GGCC'C Cuts at: 0 1259 1404 1986 3807 Size: 1259 145 582 1821 Fragments arranged by size: 1821 1259 582 145 ApaLI G'TGCA_C Cuts at: 0 520 805 3807 Size: 520 285 3002 Fragments arranged by size: 3002 520 285 ApoI r'AATT_y Cuts at: 0 276 897 3807 Size: 276 621 2910 Fragments arranged by size: 2910 621 276 AvaI C'yCGr_G Cuts at: 0 145 257 715 1397 2467 2568 3807 Size: 145 112 458 682 1070 101 1239 Fragments arranged by size: 1239 1070 682 458 145 112 101 BanI G'GyrC_C Cuts at: 0 711 1013 1210 2473 2823 3148 3807 Size: 711 302 197 1263 350 325 659 Fragments arranged by size: 1263 711 659 350 325 302 197 BanII G_rGCy'C Cuts at: 0 1259 1404 1986 2096 2129 2569 3807 Size: 1259 145 582 110 33 440 1238 Fragments arranged by size: 1259 1238 582 440 145 110 33 BbeI G_GCGC'C Cuts at: 0 3152 3807 Size: 3152 655 BbsI GAAGACnn'nnnn_ Cuts at: 0 1844 2162 2711 2796 2864 3359 3807 Size: 1844 318 549 85 68 495 448 Fragments arranged by size: 1844 549 495 448 318 85 68 BbvCI CC'TCA_GC Cuts at: 0 43 400 472 757 855 1024 2906 3807 Size: 43 357 72 285 98 169 1882 901 Fragments arranged by size: 1882 901 357 285 169 98 72 43 BglI GCCn_nnn'nGGC Cuts at: 0 253 2471 3346 3720 3807 Size: 253 2218 875 374 87 Fragments arranged by size: 2218 875 374 253 87 BglII A'GATC_T Cuts at: 0 1631 3807 Size: 1631 2176 BlpI GC'TnA_GC Cuts at: 0 871 3655 3807 Size: 871 2784 152 Fragments arranged by size: 2784 871 152 Bme1580I G_kGCm'C Cuts at: 0 358 524 716 809 1018 1259 1404 1907 Size: 358 166 192 93 209 241 145 503 Cuts at: 1907 1986 2476 3450 3807 Size: 79 490 974 357 Fragments arranged by size: 974 503 490 358 357 241 209 192 166 145 93 79 BmgBI CAC'GTC Cuts at: 0 681 1482 2349 3493 3807 Size: 681 801 867 1144 314 Fragments arranged by size: 1144 867 801 681 314 BmrI ACTGGGnnnn_n' Cuts at: 0 1635 2125 2829 3807 Size: 1635 490 704 978 Fragments arranged by size: 1635 978 704 490 BmtI G_CTAG'C Cuts at: 0 1207 3807 Size: 1207 2600 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3561 3807 Size: 3561 246 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 508 676 1006 1459 2233 2755 3067 Size: 388 120 168 330 453 774 522 312 Cuts at: 3067 3213 3801 3807 Size: 146 588 6 Fragments arranged by size: 774 588 522 453 388 330 312 168 146 120 6 Bpu10I CC'TnA_GC Cuts at: 0 43 400 472 757 855 1024 2906 3807 Size: 43 357 72 285 98 169 1882 901 Fragments arranged by size: 1882 901 357 285 169 98 72 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 351 1576 1894 2386 3247 3807 Size: 351 1225 318 492 861 560 Fragments arranged by size: 1225 861 560 492 351 318 BsaI GGTCTCn'nnnn_ Cuts at: 0 2578 3807 Size: 2578 1229 BsaAI yAC'GTr Cuts at: 0 810 1971 2148 3807 Size: 810 1161 177 1659 Fragments arranged by size: 1659 1161 810 177 BsaHI Gr'CG_yC Cuts at: 0 2597 3149 3807 Size: 2597 552 658 Fragments arranged by size: 2597 658 552 BsaWI w'CCGG_w Cuts at: 0 280 3500 3807 Size: 280 3220 307 Fragments arranged by size: 3220 307 280 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1600 1630 1777 1807 2224 2254 3211 3241 Size: 1600 30 147 30 417 30 957 30 Cuts at: 3241 3807 Size: 566 Fragments arranged by size: 1600 957 566 417 147 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 163 1396 1756 1777 2707 2782 2854 2857 Size: 163 1233 360 21 930 75 72 3 Cuts at: 2857 2881 2929 2944 3226 3427 3807 Size: 24 48 15 282 201 380 Fragments arranged by size: 1233 930 380 360 282 201 163 75 72 48 24 21 15 3 BseYI C'CCAG_C Cuts at: 0 582 623 736 1405 2406 3160 3807 Size: 582 41 113 669 1001 754 647 Fragments arranged by size: 1001 754 669 647 582 113 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 592 2101 2416 3183 3807 Size: 592 1509 315 767 624 Fragments arranged by size: 1509 767 624 592 315 BsiEI CG_ry'CG Cuts at: 0 2367 3715 3807 Size: 2367 1348 92 Fragments arranged by size: 2367 1348 92 BsiHKAI G_wGCw'C Cuts at: 0 524 542 622 809 1592 2096 3508 3807 Size: 524 18 80 187 783 504 1412 299 Fragments arranged by size: 1412 783 524 504 299 187 80 18 BsmI GAATG_Cn' Cuts at: 0 2291 3807 Size: 2291 1516 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3499 3807 Size: 3499 308 Bsp1286I G_dGCh'C Cuts at: 0 358 524 542 622 716 809 1018 1259 Size: 358 166 18 80 94 93 209 241 Cuts at: 1259 1404 1592 1907 1986 2096 2129 2476 2569 Size: 145 188 315 79 110 33 347 93 Cuts at: 2569 3450 3508 3807 Size: 881 58 299 Fragments arranged by size: 881 358 347 315 299 241 209 188 166 145 110 94 93 93 80 79 58 33 18 BspEI T'CCGG_A Cuts at: 0 280 3807 Size: 280 3527 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 149 533 620 650 1001 1320 1764 2475 Size: 149 384 87 30 351 319 444 711 Cuts at: 2475 2804 3384 3807 Size: 329 580 423 Fragments arranged by size: 711 580 444 423 384 351 329 319 149 87 30 BsrBI CCG'CTC Cuts at: 0 2049 3711 3807 Size: 2049 1662 96 Fragments arranged by size: 2049 1662 96 BsrDI GCAATG_nn' Cuts at: 0 702 2550 3750 3807 Size: 702 1848 1200 57 Fragments arranged by size: 1848 1200 702 57 BsrFI r'CCGG_y Cuts at: 0 2435 3719 3807 Size: 2435 1284 88 Fragments arranged by size: 2435 1284 88 BsrGI T'GTAC_A Cuts at: 0 725 2183 2324 3807 Size: 725 1458 141 1483 Fragments arranged by size: 1483 1458 725 141 BssHII G'CGCG_C Cuts at: 0 243 2388 3807 Size: 243 2145 1419 Fragments arranged by size: 2145 1419 243 BssSI C'ACGA_G Cuts at: 0 535 3807 Size: 535 3272 BstAPI GCAn_nnn'nTGC Cuts at: 0 606 1153 2304 3206 3807 Size: 606 547 1151 902 601 Fragments arranged by size: 1151 902 606 601 547 BstEII G'GTnAC_C Cuts at: 0 1056 3807 Size: 1056 2751 BstXI CCAn_nnnn'nTGG Cuts at: 0 654 909 2105 2667 3807 Size: 654 255 1196 562 1140 Fragments arranged by size: 1196 1140 654 562 255 BstYI r'GATC_y Cuts at: 0 684 1631 3173 3807 Size: 684 947 1542 634 Fragments arranged by size: 1542 947 684 634 Bsu36I CC'TnA_GG Cuts at: 0 930 3807 Size: 930 2877 BtgI C'CryG_G Cuts at: 0 358 1226 1554 2243 2450 2647 3807 Size: 358 868 328 689 207 197 1160 Fragments arranged by size: 1160 868 689 358 328 207 197 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 221 601 1766 3807 Size: 221 380 1165 2041 Fragments arranged by size: 2041 1165 380 221 BtsI GCAGTG_nn' Cuts at: 0 847 2071 2630 3536 3807 Size: 847 1224 559 906 271 Fragments arranged by size: 1224 906 847 559 271 DraIII CAC_nnn'GTG Cuts at: 0 1056 2119 3206 3807 Size: 1056 1063 1087 601 Fragments arranged by size: 1087 1063 1056 601 DrdI GACnn_nn'nnGTC Cuts at: 0 2800 3807 Size: 2800 1007 EaeI y'GGCC_r Cuts at: 0 155 2164 2364 2437 2669 2775 3600 3712 Size: 155 2009 200 73 232 106 825 112 Cuts at: 3712 3807 Size: 95 Fragments arranged by size: 2009 825 232 200 155 112 106 95 73 EagI C'GGCC_G Cuts at: 0 2364 3712 3807 Size: 2364 1348 95 Fragments arranged by size: 2364 1348 95 EarI CTCTTCn'nnn_ Cuts at: 0 442 953 2759 3037 3502 3807 Size: 442 511 1806 278 465 305 Fragments arranged by size: 1806 511 465 442 305 278 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2692 3807 Size: 2692 1115 EcoICRI GAG'CTC Cuts at: 0 2094 3807 Size: 2094 1713 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 292 388 508 676 901 1006 1357 Size: 133 159 96 120 168 225 105 351 Cuts at: 1357 1459 2174 2233 2755 2988 3067 3213 3682 Size: 102 715 59 522 233 79 146 469 Cuts at: 3682 3801 3807 Size: 119 6 Fragments arranged by size: 715 522 469 351 233 225 168 159 146 133 120 119 105 102 96 79 59 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 145 3807 Size: 145 3662 EcoO109I rG'GnC_Cy Cuts at: 0 1098 1263 1401 1434 1916 1982 3626 3798 Size: 1098 165 138 33 482 66 1644 172 Cuts at: 3798 3807 Size: 9 Fragments arranged by size: 1644 1098 482 172 165 138 66 33 9 EcoRI G'AATT_C Cuts at: 0 276 897 3807 Size: 276 621 2910 Fragments arranged by size: 2910 621 276 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 930 3288 3540 3807 Size: 930 2358 252 267 Fragments arranged by size: 2358 930 267 252 FspI TGC'GCA Cuts at: 0 1705 3807 Size: 1705 2102 HaeII r_GCGC'y Cuts at: 0 3152 3194 3788 3807 Size: 3152 42 594 19 Fragments arranged by size: 3152 594 42 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 301 333 1490 1522 1600 1632 2326 2358 Size: 301 32 1157 32 78 32 694 32 Cuts at: 2358 2556 2588 3807 Size: 198 32 1219 Fragments arranged by size: 1219 1157 694 301 198 78 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1698 2525 3807 Size: 1698 827 1282 Fragments arranged by size: 1698 1282 827 HindIII A'AGCT_T Cuts at: 0 1673 3807 Size: 1673 2134 KasI G'GCGC_C Cuts at: 0 3148 3807 Size: 3148 659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 926 1874 2369 3533 3572 3807 Size: 926 948 495 1164 39 235 Fragments arranged by size: 1164 948 926 495 235 39 MscI TGG'CCA Cuts at: 0 2777 3602 3807 Size: 2777 825 205 Fragments arranged by size: 2777 825 205 MslI CAynn'nnrTG Cuts at: 0 13 388 1858 3619 3807 Size: 13 375 1470 1761 188 Fragments arranged by size: 1761 1470 375 188 13 MspA1I CmG'CkG Cuts at: 0 47 345 360 1159 1197 1237 1243 1409 Size: 47 298 15 799 38 40 6 166 Cuts at: 1409 1491 1613 2649 2685 2905 3767 3807 Size: 82 122 1036 36 220 862 40 Fragments arranged by size: 1036 862 799 298 220 166 122 82 47 40 40 38 36 15 6 NaeI GCC'GGC Cuts at: 0 3721 3807 Size: 3721 86 NarI GG'CG_CC Cuts at: 0 3149 3807 Size: 3149 658 NgoMIV G'CCGG_C Cuts at: 0 3719 3807 Size: 3719 88 NheI G'CTAG_C Cuts at: 0 1203 3807 Size: 1203 2604 NotI GC'GGCC_GC Cuts at: 0 3712 3807 Size: 3712 95 NspI r_CATG'y Cuts at: 0 387 565 1347 3618 3693 3807 Size: 387 178 782 2271 75 114 Fragments arranged by size: 2271 782 387 178 114 75 PasI CC'CwG_GG Cuts at: 0 3037 3807 Size: 3037 770 PciI A'CATG_T Cuts at: 0 3614 3807 Size: 3614 193 PflMI CCAn_nnn'nTGG Cuts at: 0 1437 3807 Size: 1437 2370 PfoI T'CCnGG_A Cuts at: 0 323 1100 1736 2578 3807 Size: 323 777 636 842 1229 Fragments arranged by size: 1229 842 777 636 323 PmlI CAC'GTG Cuts at: 0 810 2148 3807 Size: 810 1338 1659 Fragments arranged by size: 1659 1338 810 PpuMI rG'GwC_Cy Cuts at: 0 1098 1263 1916 3626 3807 Size: 1098 165 653 1710 181 Fragments arranged by size: 1710 1098 653 181 165 PspOMI G'GGCC_C Cuts at: 0 1255 1400 1982 3807 Size: 1255 145 582 1825 Fragments arranged by size: 1825 1255 582 145 PspXI vC'TCGA_Gb Cuts at: 0 2467 3807 Size: 2467 1340 PstI C_TGCA'G Cuts at: 0 167 530 602 1012 1190 1236 1242 1248 Size: 167 363 72 410 178 46 6 6 Cuts at: 1248 1775 2300 2564 2815 3807 Size: 527 525 264 251 992 Fragments arranged by size: 992 527 525 410 363 264 251 178 167 72 46 6 6 PvuII CAG'CTG Cuts at: 0 345 1197 1237 1243 1491 2685 3807 Size: 345 852 40 6 248 1194 1122 Fragments arranged by size: 1194 1122 852 345 248 40 6 SacI G_AGCT'C Cuts at: 0 2096 3807 Size: 2096 1711 SacII CC_GC'GG Cuts at: 0 361 2650 3807 Size: 361 2289 1157 Fragments arranged by size: 2289 1157 361 SanDI GG'GwC_CC Cuts at: 0 3626 3807 Size: 3626 181 SapI GCTCTTCn'nnn_ Cuts at: 0 442 3807 Size: 442 3365 SbfI CC_TGCA'GG Cuts at: 0 1775 3807 Size: 1775 2032 SexAI A'CCwGG_T Cuts at: 0 1052 1124 3807 Size: 1052 72 2683 Fragments arranged by size: 2683 1052 72 SfcI C'TryA_G Cuts at: 0 163 526 598 1008 1186 1232 1238 1244 Size: 163 363 72 410 178 46 6 6 Cuts at: 1244 1771 2296 2560 2811 3384 3807 Size: 527 525 264 251 573 423 Fragments arranged by size: 573 527 525 423 410 363 264 251 178 163 72 46 6 6 SfoI GGC'GCC Cuts at: 0 3150 3807 Size: 3150 657 SmaI CCC'GGG Cuts at: 0 1399 2570 3807 Size: 1399 1171 1237 Fragments arranged by size: 1399 1237 1171 SmlI C'TyrA_G Cuts at: 0 330 1591 1909 2401 2467 3262 3807 Size: 330 1261 318 492 66 795 545 Fragments arranged by size: 1261 795 545 492 330 318 66 SnaBI TAC'GTA Cuts at: 0 1971 3807 Size: 1971 1836 SphI G_CATG'C Cuts at: 0 565 3807 Size: 565 3242 SrfI GCCC'GGGC Cuts at: 0 1399 3807 Size: 1399 2408 StuI AGG'CCT Cuts at: 0 3667 3807 Size: 3667 140 StyI C'CwwG_G Cuts at: 0 1259 1930 2009 2264 3698 3807 Size: 1259 671 79 255 1434 109 Fragments arranged by size: 1434 1259 671 255 109 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 637 646 792 1705 1967 2107 2811 3300 Size: 637 9 146 913 262 140 704 489 Cuts at: 3300 3423 3609 3807 Size: 123 186 198 Fragments arranged by size: 913 704 637 489 262 198 186 146 140 123 9 TatI w'GTAC_w Cuts at: 0 725 2183 2324 2951 3476 3807 Size: 725 1458 141 627 525 331 Fragments arranged by size: 1458 725 627 525 331 141 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1851 1883 2926 2958 3482 3514 3679 3711 Size: 1851 32 1043 32 524 32 165 32 Cuts at: 3711 3807 Size: 96 Fragments arranged by size: 1851 1043 524 165 96 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 384 981 1266 3807 Size: 384 597 285 2541 Fragments arranged by size: 2541 597 384 285 XhoI C'TCGA_G Cuts at: 0 2467 3807 Size: 2467 1340 XmaI C'CCGG_G Cuts at: 0 1397 2568 3807 Size: 1397 1171 1239 Fragments arranged by size: 1397 1239 1171 XmnI GAAnn'nnTTC Cuts at: 0 3427 3807 Size: 3427 380 Enzymes that do cut: AarI AccI AcuI AflIII AhdI AleI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacI SacII SanDI SapI SbfI SexAI SfcI SfoI SmaI SmlI SnaBI SphI SrfI StuI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BsaBI BsiWI BspHI BstBI BstZ17I ClaI CspCI DraI EcoRV FseI FspAI HpaI KpnI MfeI MluI NcoI NdeI NruI NsiI PacI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SalI ScaI SfiI SgfI SgrAI SpeI SspI SwaI Tth111I XbaI ZraI