(Circular) (Six-base) MAPSORT of: pFN21AE4446.seq Check: 280 from: 1 to: 6088 pFN21AE4446 6088 bp With 182 enzymes: SgfI * October 12, 2018 18:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3431 1937 Size: 1494 4594 Acc65I G'GTAC_C Cuts at: 3409 3409 Size: 6088 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4151 5535 5803 1632 Size: 2519 1384 268 1917 Fragments arranged by size: 2519 1917 1384 268 AfeI AGC'GCT Cuts at: 3110 3110 Size: 6088 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 3007 3080 4987 5964 1203 Size: 1804 73 1907 977 1327 Fragments arranged by size: 1907 1804 1327 977 73 AgeI A'CCGG_T Cuts at: 4919 4919 Size: 6088 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6088 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2711 1184 Size: 1527 4561 AlwNI CAG_nnn'CTG Cuts at: 2305 2804 3239 3831 5403 2305 Size: 499 435 592 1572 2990 Fragments arranged by size: 2990 1572 592 499 435 ApaLI G'TGCA_C Cuts at: 4145 5301 4145 Size: 1156 4932 ApoI r'AATT_y Cuts at: 1493 2510 2744 3397 3623 3775 5884 1493 Size: 1017 234 653 226 152 2109 1697 Fragments arranged by size: 2109 1697 1017 653 234 226 152 AseI AT'TA_AT Cuts at: 160 1032 2088 3381 4642 160 Size: 872 1056 1293 1261 1606 Fragments arranged by size: 1606 1293 1261 1056 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 3413 1870 Size: 73 1470 4545 Fragments arranged by size: 4545 1470 73 BamHI G'GATC_C Cuts at: 3418 3785 3418 Size: 367 5721 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2311 2516 3409 4864 618 Size: 325 217 627 524 205 893 1455 1842 Fragments arranged by size: 1842 1455 893 627 524 325 217 205 BanII G_rGCy'C Cuts at: 1391 2829 2912 3407 1391 Size: 1438 83 495 4072 Fragments arranged by size: 4072 1438 495 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2243 928 Size: 971 344 4773 Fragments arranged by size: 4773 971 344 BbvCI CC'TCA_GC Cuts at: 2673 2673 Size: 6088 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2212 2246 4279 4313 1903 Size: 34 275 34 2033 34 3678 Fragments arranged by size: 3678 2033 275 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3980 5190 3980 Size: 1210 4878 BclI T'GATC_A Cuts at: 1548 1548 Size: 6088 BfrBI ATG'CAT Cuts at: 3130 3130 Size: 6088 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3244 4699 136 Size: 107 122 71 631 605 1572 1455 1525 Fragments arranged by size: 1572 1525 1455 631 605 122 107 71 BglII A'GATC_T Cuts at: 6083 6083 Size: 6088 BlpI GC'TnA_GC Cuts at: 2823 3505 2823 Size: 682 5406 Bme1580I G_kGCm'C Cuts at: 1790 4149 5305 1790 Size: 2359 1156 2573 Fragments arranged by size: 2573 2359 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6088 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3819 4777 442 Size: 416 53 83 399 24 2402 958 1753 Fragments arranged by size: 2402 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2277 1056 Size: 1221 4867 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2301 2826 4748 1239 Size: 1062 525 1922 2579 Fragments arranged by size: 2579 1922 1062 525 Bpu10I CC'TnA_GC Cuts at: 2673 2673 Size: 6088 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4213 5078 5376 4213 Size: 865 298 4925 Fragments arranged by size: 4925 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2615 3025 4751 882 Size: 617 1116 410 1726 2219 Fragments arranged by size: 2219 1726 1116 617 410 BsaAI yAC'GTr Cuts at: 493 1204 3879 493 Size: 711 2675 2702 Fragments arranged by size: 2702 2675 711 BsaBI GATnn'nnATC Cuts at: 6082 6082 Size: 6088 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2967 3895 4277 4912 Size: 53 83 186 936 1434 928 382 635 Cuts at: 4912 275 Size: 1451 Fragments arranged by size: 1451 1434 936 928 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4521 4919 5193 5340 5751 4521 Size: 398 274 147 411 4858 Fragments arranged by size: 4858 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5863 Fragments arranged by size: 5863 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6088 BseYI C'CCAG_C Cuts at: 2773 5291 5787 2773 Size: 2518 496 3074 Fragments arranged by size: 3074 2518 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2318 2651 1163 Size: 573 582 333 4600 Fragments arranged by size: 4600 582 573 333 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3097 3538 4299 5327 1540 Size: 275 186 1096 441 761 1028 2301 Fragments arranged by size: 2301 1096 1028 761 441 275 186 BsiHKAI G_wGCw'C Cuts at: 2440 4149 4234 5305 2440 Size: 1709 85 1071 3223 Fragments arranged by size: 3223 1709 1071 85 BsmI GAATG_Cn' Cuts at: 3599 3692 3599 Size: 93 5995 BsmBI CGTCTCn'nnnn_ Cuts at: 2504 2504 Size: 6088 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2440 2829 2912 3407 4149 4234 5305 Size: 399 650 389 83 495 742 85 1071 Cuts at: 5305 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 650 495 399 389 85 83 BspEI T'CCGG_A Cuts at: 5751 5751 Size: 6088 BspHI T'CATG_A Cuts at: 3977 5707 3977 Size: 1730 4358 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3126 3443 844 Size: 2282 317 3489 Fragments arranged by size: 3489 2282 317 BsrBI CCG'CTC Cuts at: 1394 1823 3975 1394 Size: 429 2152 3507 Fragments arranged by size: 3507 2152 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2874 4583 4757 65 Size: 1183 1626 1709 174 1396 Fragments arranged by size: 1709 1626 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2759 4732 4919 1700 Size: 110 949 1973 187 2869 Fragments arranged by size: 2869 1973 949 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6088 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6088 BssSI C'ACGA_G Cuts at: 2184 4148 5160 2184 Size: 1964 1012 3112 Fragments arranged by size: 3112 1964 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3253 3365 794 Size: 2459 112 3517 Fragments arranged by size: 3517 2459 112 BstBI TT'CG_AA Cuts at: 4892 4892 Size: 6088 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6088 BstYI r'GATC_y Cuts at: 1972 2723 3418 3785 4170 4187 5639 5725 5737 Size: 751 695 367 385 17 1452 86 12 Cuts at: 5737 6083 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 751 695 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5536 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5349 Fragments arranged by size: 5349 683 56 BtsI GCAGTG_nn' Cuts at: 3604 4398 4418 3604 Size: 794 20 5274 Fragments arranged by size: 5274 794 20 ClaI AT'CG_AT Cuts at: 1554 2571 1554 Size: 1017 5071 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6053 DraI TTT'AAA Cuts at: 3392 3392 Size: 6088 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5095 809 Size: 507 3779 1802 Fragments arranged by size: 3779 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3535 4424 6053 Size: 54 1425 50 275 18 1705 889 1629 Cuts at: 6053 8 Size: 43 Fragments arranged by size: 1705 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3535 1537 Size: 275 1723 4090 Fragments arranged by size: 4090 1723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2019 2745 4018 1353 Size: 666 726 1273 3423 Fragments arranged by size: 3423 1273 726 666 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4675 5049 5195 387 Size: 1064 138 3086 374 146 1280 Fragments arranged by size: 3086 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2301 2826 4151 4748 5535 5803 1239 Size: 393 669 525 1325 597 787 268 1524 Fragments arranged by size: 1524 1325 787 669 597 525 393 268 EcoO109I rG'GnC_Cy Cuts at: 3236 3236 Size: 6088 EcoRI G'AATT_C Cuts at: 3397 3775 3397 Size: 378 5710 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2137 2137 Size: 6088 FspI TGC'GCA Cuts at: 4594 4594 Size: 6088 HaeII r_GCGC'y Cuts at: 1678 3112 3168 5235 1678 Size: 1434 56 2067 2531 Fragments arranged by size: 2531 2067 1434 56 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2799 2831 4729 4761 4803 Size: 32 135 32 1227 32 1898 32 42 Cuts at: 4803 4835 1373 Size: 32 2626 Fragments arranged by size: 2626 1898 1227 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3432 3678 669 Size: 988 281 1494 246 3079 Fragments arranged by size: 3079 1494 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6088 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KpnI G_GTAC'C Cuts at: 3413 3413 Size: 6088 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2760 5202 5386 1305 Size: 1455 2442 184 2007 Fragments arranged by size: 2442 2007 1455 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4266 Fragments arranged by size: 4266 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3006 3135 4046 4405 4564 518 Size: 648 1840 129 911 359 159 2042 Fragments arranged by size: 2042 1840 911 648 359 159 129 MspA1I CmG'CkG Cuts at: 1606 2652 3504 4181 5329 5574 1606 Size: 1046 852 677 1148 245 2120 Fragments arranged by size: 2120 1148 1046 852 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5536 NdeI CA'TA_TG Cuts at: 387 3068 387 Size: 2681 3407 NheI G'CTAG_C Cuts at: 1052 2273 1052 Size: 1221 4867 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NsiI A_TGCA'T Cuts at: 3132 3132 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 2692 3011 3084 3134 3446 4991 1132 Size: 97 1463 319 73 50 312 1545 2229 Fragments arranged by size: 2229 1545 1463 319 312 97 73 50 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6088 PciI A'CATG_T Cuts at: 3007 3080 4987 3007 Size: 73 1907 4108 Fragments arranged by size: 4108 1907 73 PflMI CCAn_nnn'nTGG Cuts at: 1108 3069 1108 Size: 1961 4127 PfoI T'CCnGG_A Cuts at: 2719 2719 Size: 6088 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6088 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PstI C_TGCA'G Cuts at: 830 3440 830 Size: 2610 3478 PvuI CG_AT'CG Cuts at: 2001 3097 2001 Size: 1096 4992 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SbfI CC_TGCA'GG Cuts at: 3440 3440 Size: 6088 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SfcI C'TryA_G Cuts at: 826 1045 3436 4571 5252 5443 826 Size: 219 2391 1135 681 191 1471 Fragments arranged by size: 2391 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 2759 1700 Size: 1059 5029 SmaI CCC'GGG Cuts at: 1872 3415 1872 Size: 1543 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4192 5093 5355 820 Size: 197 926 2249 901 262 1553 Fragments arranged by size: 2249 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3879 493 Size: 3386 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 3446 1132 Size: 2314 3774 SspI AAT'ATT Cuts at: 5 52 4012 5 Size: 47 3960 2081 Fragments arranged by size: 3960 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6088 StyI C'CwwG_G Cuts at: 513 1065 2064 3527 513 Size: 552 999 1463 3074 Fragments arranged by size: 3074 1463 999 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3125 4115 4132 4285 4470 4915 325 Size: 1255 1545 990 17 153 185 445 1498 Fragments arranged by size: 1545 1498 1255 990 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4334 6046 96 Size: 274 80 33 51 1443 2357 1712 138 Fragments arranged by size: 2357 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6048 6080 6048 Size: 32 6056 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6088 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2071 2452 2071 Size: 381 5707 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6088 XmaI C'CCGG_G Cuts at: 1870 3413 1870 Size: 1543 4545 XmnI GAAnn'nnTTC Cuts at: 4217 4217 Size: 6088 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI